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9 results on '"Han, Jieyun"'

7. Caffeine Content and Related Gene Expression: Novel Insight into Caffeine Metabolism in CamelliaPlants Containing Low, Normal, and High Caffeine Concentrations

Author

Zhu, Biying, Chen, Lin-Bo, Lu, Mengqian, Zhang, Jing, Han, Jieyun, Deng, Wei-Wei, and Zhang, Zheng-Zhu

Abstract

Caffeine is a crucial secondary metabolic product in tea plants. Although the presence of caffeine in tea plants has been identified, the molecular mechanisms regulating relevant caffeine metabolism remain unclear. For the elucidation of the caffeine biosynthesis and catabolism in Camelliaplants, fresh, germinated leaves from four Camelliaplants with low (2), normal (1), and high (1) caffeine concentrations, namely, low-caffeine tea 1 (LCT1, Camellia crassicolumna), low-caffeine tea 2 (LCT2, C. crassicolumna), Shuchazao (SCZ, C. sinensis), and Yunkang 43 (YK43, C. sinensis) were used in this research. Transcriptome and purine alkaloids analyses of these Camellialeaves were performed using RNA-Seq and liquid chromatography–mass spectrometry (LC–MS). Moreover, 15N-caffeine tracing was performed to determine the metabolic fate of caffeine in leaves of these plants. Caffeine content was correlated with related gene expression levels, and a quantitative real-time (qRT) PCR analysis of specific genes showed a consistent tendency with the obtained transcriptomic analysis. On the basis of the results of stable isotope-labeled tracer experiments, we discovered a degradation pathway of caffeine to theobromine. These findings could assist researchers in understanding the caffeine-related mechanisms in Camelliaplants containing low, normal, and high caffeine content and be applied to caffeine regulation and breeding improvement in future research.

Published
2019
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9. Uncovering tea-specific secondary metabolism using transcriptomic and metabolomic analyses in grafts of <italic>Camellia sinensis</italic> and <italic>C</italic>. <italic>oleifera</italic>.

Author

Deng, Wei-Wei, Han, Jieyun, Fan, Yanbing, Tai, Yuling, Zhu, Biying, Lu, Mengqian, Wang, Rangjian, Wan, Xiaochun, and Zhang, Zheng-Zhu

Subjects
PLANT metabolites, FLAVONOIDS, GENE expression in plants, METABOLOMICS, GENETIC transcription in plants
Abstract

Camellia sinensis (L.) Kuntze and Camellia oleifera C. Abel (Theaceae) are closely related perennial woody shrubs, but the accumulation of metabolites and gene expression patterns are quite different between these two species. In order to understand the mechanisms behind the accumulation and biosynthesis of tea-specific secondary metabolites and the key genes that regulate their target pathways, 1-year-old clone cuttings of C. sinensis and C. oleifera were grafted in both directions, and self-grafted C. sinensis were used as controls. The transcriptomes and metabolomes of leaves and roots from the grafts were analyzed. We found that 1375 unigenes were up-regulated in the leaves of the CS-CO grafts (C. sinensis scion, C. oleifera stock), while 2437 unigenes were down-regulated. OPLS-DA models established for 7230 and 3223 mass spectra peaks were obtained in the positive and negative modes by LC-MS detection. Association analysis of the secondary metabolism pathways was performed, and the relative gene expressions of 14 genes from the transcriptome screening were verified by qRT-PCR. Among the differential metabolites screened and identified, we found that the relative levels of theanine and caffeine decreased significantly, and that many of the genes in these metabolic pathways were also down-regulated. In contrast, the levels of flavonoids apparently increased, and the expression of related genes in the flavonoid biosynthetic pathway were mostly up-regulated. [ABSTRACT FROM AUTHOR]

Published
2018
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