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4. Cross-biome soil viruses as an important reservoir of virulence genes

Author

Bi, L, Han, L-L, Du, S, Yu, D-T, He, J-Z, Zhang, L-M, Hu, H-W, Bi, L, Han, L-L, Du, S, Yu, D-T, He, J-Z, Zhang, L-M, and Hu, H-W

Abstract

Viruses can significantly influence the composition and functions of their host communities and enhance host pathogenicity via the transport of virus-encoded virulence genes. However, the contribution of viral communities to the dissemination of virulence genes across various biomes across a large scale is largely unknown. Here, we constructed 29,283 soil viral contigs (SVCs) from viral size fraction metagenomes and public databases. A total of 1310 virulence genes were identified from 1164 SVCs in a wide variety of soil biomes, including grassland, agricultural and forest soils. The virulence gene gmd was the most abundant one, followed by csrA, evpJ, and pblA. A great proportion of viruses encoding virulence genes were uncharacterized. Virus-host linkage analysis revealed that most viruses were linked to only one bacterial genus, whereas several SVCs were associated with more than one bacterial genus and even two bacterial phyla, suggesting the potential risk of spreading virulence genes across different bacterial communities via viruses. Altogether, we provided new evidence for the prevalence of virulence genes in soil viruses across biomes, which advanced our understanding of the potential role of soil viruses in driving the pathogenesis of their hosts in terrestrial ecosystems.

Published
2023

5. The effect of small molecule inhibitor NSC348884 on nucleophosmin 1-mutated acute myeloid leukemia cells.

Author

ZHU, J.-F., HAN, L.-L., MA, Y., WANG, Y.-X., and ZHANG, F.

Abstract

OBJECTIVE: Nucleophosmin 1 (NPM1) is a common shuttling protein. Mutation in the NPM1 gene is the most frequent gene alteration in acute myeloid leukemia (AML). This study aims to explore the inhibitory effects of small molecule NSC348884 on wild-type and NPM1-mutated AML cells. MATERIALS AND METHODS: Immunofluorescence was used to determine the intracellular localization of NPM1 protein in wild-type (OCIAML2) and NPM1-mutated (OCI-AML3) AML cell lines. The oligomerization state of NPM1 was assessed by Western blot analysis, and the inhibitory effect of NSC348884 on the proliferation of AML cells was evaluated by Cell-counting kit-8 (CCK-8). Flow cytometry was used to detect the proapoptotic effect of NSC348884 on AML cells. RESULTS: Western blot results showed a significant reduction in the levels of the oligomeric NPM1 protein after the treatment with NSC348884. NSC348884 had an inhibitory effect on the proliferation of both wild-type and NPM1-mutant AML cells. The inhibitory effect on OCI-AML3 cells was stronger, compared to OCI-AML2 cells. Flow cytometry showed that NSC348884 could significantly induce AML cell apoptosis and had a stronger proapoptotic effect on OCI-AML3 cells. CONCLUSIONS: NSC348884 had inhibitory and proapoptotic effects on both wild-type and NPM1-mutated AML cells. The effect of NSC348884 on AML cells, carrying NPM1 mutation was significantly stronger. [ABSTRACT FROM AUTHOR]

Published
2023

6. Distribution Characteristics of Soil Viruses Under Different Precipitation Gradients on the Qinghai-Tibet Plateau

Author

Cao, M-M, Liu, S-Y, Bi, L, Chen, S-J, Wu, H-Y, Ge, Y, Han, B, Zhang, L-M, He, J-Z, Han, L-L, Cao, M-M, Liu, S-Y, Bi, L, Chen, S-J, Wu, H-Y, Ge, Y, Han, B, Zhang, L-M, He, J-Z, and Han, L-L

Abstract

Viruses are extremely abundant in the soil environment and have potential roles in impacting on microbial population, evolution, and nutrient biogeochemical cycles. However, how environment and climate changes affect soil viruses is still poorly understood. Here, a metagenomic approach was used to investigate the distribution, diversity, and potential biogeochemical impacts of DNA viruses in 12 grassland soils under three precipitation gradients on the Qinghai-Tibet Plateau, which is one of the most sensitive areas to climate change. A total of 557 viral operational taxonomic units were obtained, spanning 152 viral families from the 30 metagenomes. Both virus-like particles (VLPs) and microbial abundance increased with average annual precipitation. A significant positive correlation of VLP counts was observed with soil water content, total carbon, total nitrogen, soil organic matter, and total phosphorus. Among these biological and abiotic factors, SWC mainly contributed to the variability in VLP abundance. The order Caudovirales (70.1% of the identified viral order) was the predominant viral type in soils from the Qinghai-Tibet Plateau, with the Siphoviridae family being the most abundant. Remarkably, abundant auxiliary carbohydrate-active enzyme (CAZyme) genes represented by glycoside hydrolases were identified, indicating that soil viruses may play a potential role in the carbon cycle on the Qinghai-Tibet Plateau. There were more diverse hosts and abundant CAZyme genes in soil with moderate precipitation. Our study provides a strong evidence that changes in precipitation impact not only viral abundance and virus-host interactions in soil but also the viral functional potential, especially carbon cycling.

Published
2022

7. Distribution of soil viruses across China and their potential role in phosphorous metabolism

Author

Han, L-L, Yu, D-T, Bi, L, Du, S, Silveira, C, Guemes, AGC, Zhang, L-M, He, J-Z, Rohwer, F, Han, L-L, Yu, D-T, Bi, L, Du, S, Silveira, C, Guemes, AGC, Zhang, L-M, He, J-Z, and Rohwer, F

Abstract

BACKGROUND: Viruses are the most abundant biological entities on the planet and drive biogeochemical cycling on a global scale. Our understanding of biogeography of soil viruses and their ecological functions lags significantly behind that of Bacteria and Fungi. Here, a viromic approach was used to investigate the distribution and ecological functions of viruses from 19 soils across China. RESULTS: Soil viral community were clustered more significantly by geographical location than type of soil (agricultural and natural). Three clusters of viral communities were identified from North, Southeast and Southwest regions; these clusters differentiated using taxonomic composition and were mainly driven by geographic location and climate factors. A total of 972 viral populations (vOTUs) were detected spanning 23 viral families from the 19 viromes. Phylogenetic analyses of the phoH gene showed a remarkable diversity and the distribution of viral phoH genes was more dependent on the environment. Notably, five proteins involved in phosphorus (P) metabolism-related nucleotide synthesis functions, including dUTPase, MazG, PhoH, Thymidylate synthase complementing protein (Thy1), and Ribonucleoside reductase (RNR), were mainly identified in agricultural soils. CONCLUSIONS: The present work revealed that soil viral communities were distributed across China according to geographical location and climate factors. In addition, P metabolism genes encoded by these viruses probably drive the synthesis of nucleotides for their own genomes inside bacterial hosts, thereby affecting P cycling in the soil ecosystems.

Published
2022

10. Host selection shapes crop microbiome assembly and network complexity

Author

Xiong, C, Zhu, Y-G, Wang, J-T, Singh, B, Han, L-L, Shen, J-P, Li, P-P, Wang, G-B, Wu, C-F, Ge, A-H, Zhang, L-M, He, J-Z, Xiong, C, Zhu, Y-G, Wang, J-T, Singh, B, Han, L-L, Shen, J-P, Li, P-P, Wang, G-B, Wu, C-F, Ge, A-H, Zhang, L-M, and He, J-Z

Abstract

Plant microbiomes are essential to host health and productivity but the ecological processes that govern crop microbiome assembly are not fully known. Here we examined bacterial communities across 684 samples from soils (rhizosphere and bulk soil) and multiple compartment niches (rhizoplane, root endosphere, phylloplane, and leaf endosphere) in maize (Zea mays)-wheat (Triticum aestivum)/barley (Hordeum vulgare) rotation system under different fertilization practices at two contrasting sites. Our results demonstrate that microbiome assembly along the soil-plant continuum is shaped predominantly by compartment niche and host species rather than by site or fertilization practice. From soils to epiphytes to endophytes, host selection pressure sequentially increased and bacterial diversity and network complexity consequently reduced, with the strongest host effect in leaf endosphere. Source tracking indicates that crop microbiome is mainly derived from soils and gradually enriched and filtered at different plant compartment niches. Moreover, crop microbiomes were dominated by a few dominant taxa (c. 0.5% of bacterial phylotypes), with bacilli identified as the important biomarker taxa for wheat and barley and Methylobacteriaceae for maize. Our work provides comprehensive empirical evidence on host selection, potential sources and enrichment processes for crop microbiome assembly, and has important implications for future crop management and manipulation of crop microbiome for sustainable agriculture.

Published
2021

11. Rare taxa maintain the stability of crop mycobiomes and ecosystem functions

Author

Xiong, C, He, J-Z, Singh, BK, Zhu, Y-G, Wang, J-T, Li, P-P, Zhang, Q-B, Han, L-L, Shen, J-P, Ge, A-H, Wu, C-F, Zhang, L-M, Xiong, C, He, J-Z, Singh, BK, Zhu, Y-G, Wang, J-T, Li, P-P, Zhang, Q-B, Han, L-L, Shen, J-P, Ge, A-H, Wu, C-F, and Zhang, L-M

Abstract

Plants harbour highly diverse mycobiomes which sustain essential functions for host health and productivity. However, ecological processes that govern the plant–mycobiome assembly, interactions and their impact on ecosystem functions remain poorly known. Here we characterized the ecological role and community assembly of both abundant and rare fungal taxa along the soil–plant continuums (rhizosphere, phyllosphere and endosphere) in the maize–wheat/barley rotation system under different fertilization practices at two contrasting sites. Our results indicate that mycobiome assembly is shaped predominantly by compartment niche and host species rather than by environmental factors. Moreover, crop-associated fungal communities are dominated by few abundant taxa mainly belonging to Sordariomycetes and Dothideomycetes, while the majority of diversity within mycobiomes are represented by rare taxa. For plant compartments, the abundant sub-community is mainly determined by stochastic processes. In contrast, the rare sub-community is more sensitive to host selection and mainly governed by deterministic processes. Furthermore, our results demonstrate that rare taxa play an important role in fungal co-occurrence network and ecosystem functioning like crop yield and soil enzyme activities. These results significantly advance our understanding of crop mycobiome assembly and highlight the key role of rare taxa in sustaining the stability of crop mycobiomes and ecosystem functions.

Published
2021

12. Generalist Taxa Shape Fungal Community Structure in Cropping Ecosystems

Author

Wang, J-T, Shen, J-P, Zhang, L-M, Singh, BK, Delgado-Baquerizo, M, Hu, H-W, Han, L-L, Wei, W-X, Fang, Y-T, He, J-Z, Wang, J-T, Shen, J-P, Zhang, L-M, Singh, BK, Delgado-Baquerizo, M, Hu, H-W, Han, L-L, Wei, W-X, Fang, Y-T, and He, J-Z

Abstract

Fungi regulate nutrient cycling, decomposition, symbiosis, and pathogenicity in cropland soils. However, the relative importance of generalist and specialist taxa in structuring soil fungal community remains largely unresolved. We hypothesized that generalist fungi, which are adaptable to various environmental conditions, could potentially dominate the community and become the basis for fungal coexisting networks in cropping systems. In this study, we identified the generalist and habitat specialist fungi in cropland soils across a 2,200 kms environmental gradient, including three bioclimatic regions (subtropical, warm temperate, and temperate). A few fungal taxa in our database were classified as generalist taxa (~1%). These generalists accounted for >35% of the relative abundance of all fungal populations, and most of them are Ascomycota and potentially pathotrophic. Compared to the specialist taxa (5-17% of all phylotypes in three regions), generalists had a higher degree of connectivity and were often identified as hub within the network. Structural equation modeling provided further evidence that after accounting for spatial and climatic/edaphic factors, generalists had larger contributions to the fungal coexistence pattern than habitat specialists. Taken together, our study provided evidence that generalist taxa are crucial components for fungal community structure. The knowledge of generalists can provide important implication for understanding the ecological preference of fungal groups in cropland systems.

Published
2021

13. Unveiling anneal hardening in dilute Al-doped AlxCoCrFeMnNi (x=0, 0.1) high-entropy alloys

Author

Cheng, Q., Xu, X. D., Xie, P., Han, L. L., He, J. Y., Li, Xiaoqing, Zhang, J., Li, Z. T., Li, Y. P., Liu, B., Nieh, T. G., Chen, M. W., Chen, J. H., Cheng, Q., Xu, X. D., Xie, P., Han, L. L., He, J. Y., Li, Xiaoqing, Zhang, J., Li, Z. T., Li, Y. P., Liu, B., Nieh, T. G., Chen, M. W., and Chen, J. H.

Abstract

Anneal hardening has been one of the approaches to improve mechanical properties of solid solution alloys with the face-centered cubic (FCC) structure, whereby a considerable strengthening can be attained by annealing of cold-worked alloys below the recrystallization temperature (T-rx). Microscopically, this hardening effect has been ascribed to several mechanisms, i.e. solute segregation to defects (dislocation and stacking fault) and short-range chemical ordering, etc. However, none of these mechanisms can well explain the anneal hardening recently observed in phase-pure and coarse-grained FCC-structured high-entropy alloys (HEAs). Here we report the observations, using high-resolution electron channeling contrast imaging and transmission electron microscopy, of profuse and stable dislocation substructures in a cold-rolled CoCrFeMnNi HEA subject to an annealing below T-rx. The dislocation substructures are observed to be thermally stable up to T-rx, which could arise from the chemical complexity of the high-entropy system where certain elemental diffusion retardation occurs. The microstructure feature is markedly different from that of conventional dilute solid solution alloys, in which dislocation substructures gradually vanish by recovery during annealing, leading to a strength drop. Furthermore, dilute addition of 2 at.% Al leads to a reduction in both microhardness and yield strength of the cold-rolled and subsequently annealed (<= 500 degrees C) HEA. This Al induced softening effect, could be associated with the anisotropic formation of dislocation substructure, resulting from enhanced dislocation planar slip due to glide plane softening effect. These findings suggest that the strength of HEAs can be tailored through the anneal hardening effect from dislocation substructure strengthening., QC 20220309

Published
2021
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16. Diversity and potential biogeochemical impacts of viruses in bulk and rhizosphere soils

Author

Bi, L, Yu, D-T, Du, S, Zhang, L-M, Zhang, L-Y, Wu, C-F, Xiong, C, Han, L-L, He, J-Z, Bi, L, Yu, D-T, Du, S, Zhang, L-M, Zhang, L-Y, Wu, C-F, Xiong, C, Han, L-L, and He, J-Z

Abstract

Viruses can affect microbial dynamics, metabolism and biogeochemical cycles in aquatic ecosystems. However, viral diversity and functions in agricultural soils are poorly known, especially in the rhizosphere. We used virome analysis of eight rhizosphere and bulk soils to study viral diversity and potential biogeochemical impacts in an agro-ecosystem. The order Caudovirales was the predominant viral type in agricultural soils, with Siphoviridae being the most abundant family. Phylogenetic analysis of the terminase large subunit of Caudovirales identified high viral diversity and three novel groups. Viral community composition differed significantly between bulk and rhizosphere soils. Soil pH was the main environmental driver of the viral community structure. Remarkably, abundant auxiliary carbohydrate-active enzyme (CAZyme) genes were detected in viromes, including glycoside hydrolases, carbohydrate esterases and carbohydrate-binding modules. These results demonstrate that virus-encoded putative auxiliary metabolic genes or metabolic genes that may change bacterial metabolism and indirectly contribute to biogeochemical cycling, especially carbon cycling, in agricultural soil.

Published
2020

17. Protist communities are more sensitive to nitrogen fertilization than other microorganisms in diverse agricultural soils

Author

Zhao, Z-B, He, J-Z, Geisen, S, Han, L-L, Wang, J-T, Shen, J-P, Wei, W-X, Fang, Y-T, Li, P-P, Zhang, L-M, Zhao, Z-B, He, J-Z, Geisen, S, Han, L-L, Wang, J-T, Shen, J-P, Wei, W-X, Fang, Y-T, Li, P-P, and Zhang, L-M

Abstract

BACKGROUND: Agricultural food production is at the base of food and fodder, with fertilization having fundamentally and continuously increased crop yield over the last decades. The performance of crops is intimately tied to their microbiome as they together form holobionts. The importance of the microbiome for plant performance is, however, notoriously ignored in agricultural systems as fertilization disconnects the dependency of plants for often plant-beneficial microbial processes. Moreover, we lack a holistic understanding of how fertilization regimes affect the soil microbiome. Here, we examined the effect of a 2-year fertilization regime (no nitrogen fertilization control, nitrogen fertilization, and nitrogen fertilization plus straw amendment) on entire soil microbiomes (bacteria, fungi, and protist) in three common agricultural soil types cropped with maize in two seasons. RESULTS: We found that the application of nitrogen fertilizers more strongly affected protist than bacterial and fungal communities. Nitrogen fertilization indirectly reduced protist diversity through changing abiotic properties and bacterial and fungal communities which differed between soil types and sampling seasons. Nitrogen fertilizer plus straw amendment had greater effects on soil physicochemical properties and microbiome diversity than nitrogen addition alone. Moreover, nitrogen fertilization, even more together with straw, increased soil microbiome network complexity, suggesting that the application of nitrogen fertilizers tightened soil microbiomes interactions. CONCLUSIONS: Together, our results suggest that protists are the most susceptible microbiome component to the application of nitrogen fertilizers. As protist communities also exhibit the strongest seasonal dynamics, they serve as the most sensitive bioindicators of soil changes. Changes in protist communities might have long-term effects if some of the key protist hubs that govern microbiome complexities as top microbiome

Published
2019

18. Tevatron Run II combination of the effective leptonic electroweak mixing angle

Author

Aaltonen, T., Abazov, V. M., Abbott, B., Acharya, B. S., Adams, M., Adams, T., Agnew, J. P., Alexeev, G. D., Alkhazov, G., Alton, A., Amerio, S., Amidei, D., Anastassov, A., Annovi, A., Antos, J., Apollinari, G., Appel, J. A., Arisawa, T., Artikov, A., Asaadi, J., Ashmanskas, W., Askew, A., Atkins, S., Auerbach, B., Augsten, K. K., Aurisano, A., Aushev, V., Aushev, Y., Avila, C., Azfar, F., Badaud, F., Badgett, W., Bae, T., Bagby, L., Baldin, B., Bandurin, D. V., Banerjee, S., Barbaro-Galtieri, A., Barberis, E., Baringer, P., Barnes, V. E., Barnett, B. A., Barria, P., Bartlett, J. F., Bartos, P., Bassler, U., Bauce, M., Bazterra, V., Bean, A., Bedeschi, F., Begalli, M., Behari, S., Bellantoni, L., Bellettini, G., Bellinger, J., Benjamin, D., Beretvas, A., Beri, S. B., Bernardi, G., Bernhard, R., Bertram, I., Besancon, M., Beuselinck, R., Bhat, P. C., Bhatia, S., Bhatnagar, V., Bhatti, A., Bland, K. R., Blazey, G., Blessing, S., Bloom, K., Blumenfeld, B., Bocci, A., Bodek, A., Boehnlein, A., Boline, D., Boos, E. E., Borisov, G., Bonoletto, D., Borysova, M., Boudreau, J., Boveia, A., Brandt, A., Brandt, O., Brigliadori, L., Brochmann, M., Brock, R., Bromberg, C., Bross, A., Brown, D., Brucken, E., Bu, X. B., Budagov, J. J., Budd, H. S., Buehler, M., Buescher, V., Bunichev, V., Burdin, S., Burkett, K., Busetto, G., Bussey, P., Buszello, Claus P., Butti, P., Buzatu, A., Calamba, A., Camacho-Perez, E., Camarda, S., Campanelli, M., Canelli, F., Carls, B., Carlsmith, D., Carosi, R., Carrillo, S., Casal, B., Casarsa, M., Casey, B. C. K., Castilla-Valdez, H., Castro, A., Catastini, P., Caughron, S., Cauz, D., Cavaliere, V., Cerri, A., Cerrito, L., Chakrabarti, S., Chan, K. M., Chandra, A., Chapon, E., Chen, G., Chen, Y. C., Chertok, M., Chiarelli, G., Chlachidze, G., Cho, K., Cho, S. W., Choi, S., Chokheli, D., Choudhary, B., Cihangir, S., Claes, D., Clark, A., Clarke, C., Clutter, J., Convery, M. M. E., Conway, J., Cooke, M. M., Cooper, W. E., Corbo, M., Corcoran, M., Cordelli, M., Couderc, F., Cousinou, M. -C, Cox, C. A., Cox, D. J., Cremonesi, M., Cruz, D., Cuevas, J., Culbertson, R., Cuth, J., Cutts, D., Das, A., d'Ascenzo, N., Datta, M., Davies, G., de Barbaro, P., de Jong, S. J., De La Cruz-Burelo, E., Deliot, F., Demina, R., Demortier, L., Deninno, M., Denisov, D., Denisov, S. P., D'Errico, M., Desai, S., Deterre, C., DeVaughan, K., Devoto, F., Di Canto, A., Di Ruzza, B., Diehl, H. T., Diesburg, M., Ding, P. F., Dittmann, J. R., Dominguez, A., Donati, S., D'Onofrio, M., Dorigo, M., Driutti, A., Drutskoy, A., Dubey, A., Dudko, L. V., Duperrin, A., Dutt, S., Eads, M., Ebina, K., Edgar, R., Edmunds, D., Elagin, A., Ellison, J., Elvira, V. D., Enari, Y., Erbacher, R., Errede, S., Esham, B., Evans, H., Evdokimov, A., Evdokimov, V. N., Farrington, S., Faure, A., Feng, L., Ferbel, T., Ramos, J. P. Fernandez, Fiedler, F., Field, R., Filthaut, F., Fisher, W., Fisk, H. E., Flanagan, G., Forrest, R., Fortner, M., Fox, H., Franc, J. J., Franklin, M., Freeman, J. C., Frisch, H. H., Fuess, S., Funakoshi, Y. Y., Galloni, C., Garbincius, P. H., Garcia-Bellido, A., Garcia-Gonzalez, J. A., Garfinkel, A. F., Garosi, P., Gavrilov, V., Geng, W., Gerber, C. E., Gerberich, H., Gerchtein, E., Gershtein, Y., Giagu, S., Giakoumopoulou, V., Gibson, K., Ginsburg, C. M., Ginther, G., Giokaris, N., Giromini, P., Glagolev, V., Glenzinski, D., Gogota, O., Gold, M., Goldin, D., Golossanov, A., Golovanov, G., Gomez, G., Gomez-Ceballos, G., Goncharov, M., Lopez, O. Gonzalez, Gorelov, I., Goshaw, A. T., Goulianos, K., Gramellini, E., Grannis, P. D., Greder, S., Greenlee, H., Grenier, G., Gris, Ph., Grivaz, J. -F, Grohscjean, A., Grosso-Pilcher, C., Gruenendahl, S., Gruenewald, M. W., Guillemin, T., da Costa, J. Guimaraes, Gutierrez, G., Gutierrez, P., Hahn, S. R., Haley, J., Han, J. Y., Han, L. L., Happacher, F., Hara, K., Harder, K., Hare, M., Harel, A., Harr, R. F., Harrington-Taber, T., Hatakeyama, K., Hauptman, J. M., Hays, C., Hays, J., Head, T., Hebbeker, T., Hedin, D., Hegab, H., Heinrich, J., Heinson, A. P., Heintz, U., Hensel, C., Heredia-De La Cruz, I., Hendon, M., Herner, K., Hesketh, G., Hildreth, M. D., Hirosky, R., Huang, T., Hobbs, J. D., Hocker, A., Hoeneisen, B., Hogan, J., Hohlfeld, M., Holzbauer, J. L., Hong, Z., Hopkins, W., Hou, S., Howley, I., Hubacek, Z., Hughes, R. E., Husemann, U., Hussein, M., Huston, J., Hynek, V., Iashvili, I., Ilchenko, Y., Illingworth, R., Introzzi, G., Iori, M., Ito, A. S., Ivanov, A., Jabeen, S., Jaffre, M., James, E., Jang, D., Jayasinghe, A., Jayatilaka, B., Jeon, E. J., Jeong, M. S., Jesik, R., Jiang, P., Jindariani, S., Johns, K., Johnson, E. E., Johnson, M., Jonckheere, A. A., Jones, M., Jonsson, P., Joo, K. K., Joshi, J., Jun, S. Y., Jung, A. W., Junk, T. R., Juste, A., Kajfasz, E., Kambeitz, M., Kamon, T., Karchin, P. E., Karmanov, D., Kasmi, A., Kato, Y., Katsanos, I., Kaur, M., Kehoe, R., Kermiche, S., Ketchum, W., Keung, J., Khalatyan, N., Khanov, A., Kharchilava, A., Kharzheev, Y. N., Kilminster, B., Kim, D. H., Kim, H. S., Kim, J. E., Kim, M. J., Kim, S. H., Kim, S. B., Kim, Y. J., Kim, Y. K., Kimura, N., Kirby, M., Kiselevich, I., Kohli, J. M., Kondo, K., Kong, D. J., Konigsberg, J. J., Kotwa, A. V., Kozelov, A. V., Kraus, J., Kreps, M., Kroll, J., Kruse, M., Kuhr, T., Kumar, A., Kupco, A., Kurata, M., Kurca, T., Kuzmin, V. A., Laasanen, A. T., Lammel, S., Lammers, S., Lancaster, M., Lannon, K., Latino, G., Lebrun, P., Lee, H. S., Lee, J. S., Lee, S. W., Lee, W. M., Lei, X., Lellouch, J., Leo, S., Leone, S., Lewis, J. D., Li, D., Li, H., Li, L., Li, Q. Z., Lim, J. K., Limosani, A., Lincoln, D., Linnemann, J., Lipaev, V. V., Lipeles, E., Lipton, R., Lister, A., Liu, H., Liu, Q., Liu, T., Liu, Y., Lobodenko, A., Lockwitz, S., Loginov, A., Lokajicek, M., de Sa, R. Lopes, Lucchesi, D., Luca, A., Lueck, J., Lujan, P., Lukens, P., Luna-Garcia, R., Lungu, G., Lyon, A. L., Lys, J., Lysak, R., Maciel, A. K. A., Madar, R., Madrak, R., Maestro, P., Magana-Villalba, R., Malik, S., Malyshev, V. L., Manca, G., Manousakis-Katsikakis, A., Mansour, J., Marchese, L., Margaroli, F., Marino, P., Martinez-Ortega, J., Matera, K., Mattson, M. E., Mazzacane, A., Mazzanti, P., McCarthy, R., McGivern, C. L., McNulty, R., Mehta, A., Mehtala, P., Meijer, M. M., Melnitchouk, A., Menezes, D., Mercadante, P. G., Merkin, M., Mesropian, C., Meyer, A., Meyer, J., Miao, T., Miconi, F., Mietlicki, D., Mitra, A., Miyake, H., Moed, S., Moggi, N., Mondal, N. K., Moon, C. S., Moore, R., Morello, M. J., Mukherjee, A., Mulhearn, M., Muller, Th., Murat, P., Mussini, M., Nachtman, J., Nagai, Y., Naganoma, J., Nagy, E., Nakano, I., Napier, A., Narain, M., Nayyar, R., Neal, H. A., Negret, J. P., Nett, J., Neustroev, P., Nguyen, H. T., Nigmanov, T., Nodulman, L., Noh, S. Y., Nomiella, O., Nunnemann, T., Oakes, L., Oh, S. H., Oh, Y. D., Okusawa, T., Orava, R., Orduna, J., Ortolan, L., Osman, N., Pagliarone, C., Pal, A., Palencia, E., Palni, P., Papadimitriou, V., Parashar, N., Parihar, V., Park, S. K., Parker, W., Partridge, R., Parua, N., Patwa, A., Pauletta, G., Paulini, M., Paus, C., Penning, B., Perfilov, M., Peters, Y., Petridis, K., Petrillo, G., Petroff, P., Phillips, T. J., Piacentino, G., Pianori, E., Pilot, J., Pitts, K., Plager, C., Pleier, M. -A, Podstavkov, V. M., Pondrom, L., Popov, A. V., Poprocki, S. S., Potamianos, K., Pranko, A., Prewitt, M., Price, D., Prokopenko, N., Prokoshin, F., Ptohos, F., Punzi, G., Qian, J., Quadt, A., Quinn, B., Ratoff, P. N., Razumov, I., Fernandez, I. Redondo, Renton, P., Rescigno, M., Rimondi, F., Ripp-Baudot, I., Ristori, L., Rizatdinova, F., Robson, A., Rodriguez, T., Rolli, S., Rominsky, M., Ronzani, M., Roser, R., Rosner, J. L., Ross, A., Royon, C., Rubinov, P., Ruchti, R., Ruffini, F., Ruiz, A., Russ, J., Rusu, V. V., Sajot, G., Sakumoto, W. K., Sakurai, Y., Sanchez-Hernandez, A., Sanders, M. M. P., Santi, L., Santos, A. S., Sato, K., Savage, C., Saveliev, V., Savitskyi, M., Savoy-Navarro, A., Sawyer, L., Scanlon, T., Schamberger, R. D., Scheglov, Y., Schellman, H., Schlabach, P., Schmidt, E. E., Schott, M., Schwanenberger, C., Schwarz, T., Schwienhorst, R., Scodellaro, L., Scuri, F., Seidel, S., Seiya, Y., Sekaric, J., Semenov, A., Severini, H., Sforza, F., Shabalina, E., Shalhout, S. Z., Sham, V., Shaw, S., Shchukin, A. A., Shears, T., Shepard, P. F., Shimojima, M., Shkola, O., Shochet, M., Shreyber-Tecker, I., Simak, V., Simonenko, A., Skubic, P., Slattery, P., Sliwa, K., Smith, J. R., Snider, F. D., Snow, C. R., Snow, J., Snyder, S., Soldner-Rembold, S., Song, H., Sonnenschein, L., Sorin, V., Soustruznik, K., St Denis, R., Stancari, M., Stark, J., Stetaniuk, N., Stentz, D., Stoyanova, D. A., Strauss, M., Strologas, J., Sudo, Y., Sukhanov, A., Suslov, I., Suter, L., Svoisky, P., Takemasa, K., Takeuchi, Y., Tang, J., Tecchio, M., Teng, P. K., Thom, J., Thomson, E., Thukral, V., Titov, M., Toback, D., Tokar, S., Tokmenin, V. V., Tollefson, K., Tomura, T., Tonelli, D., Torre, S., Torretta, D., Totaro, P., Trovato, M., Tsai, Y. -T, Tsybychev, D., Tuchming, B., Tully, C., Ukegawa, F. F., Uozumi, S., Uvarov, L., Uvarov, S., Uzunyan, S., Van Kooten, R., van Leeuwen, W. M., Varelas, N., Varnes, E. W., Vasilyev, I. A., Vazquez, F., Velev, G., Vellidis, C., Verkheev, A. Y., Vernieri, C., Vertogradov, L. S., Verzocchi, M., Vesterinen, M., Vidal, M., Vilanova, D., Vilar, R., Vizan, J., Vogel, M., Vokac, P., Volpi, G., Wagner, P., Wahl, H. D., Wallny, R., Wang, C., Wang, M. 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F., Harrington-Taber, T., Hatakeyama, K., Hauptman, J. M., Hays, C., Hays, J., Head, T., Hebbeker, T., Hedin, D., Hegab, H., Heinrich, J., Heinson, A. P., Heintz, U., Hensel, C., Heredia-De La Cruz, I., Hendon, M., Herner, K., Hesketh, G., Hildreth, M. D., Hirosky, R., Huang, T., Hobbs, J. D., Hocker, A., Hoeneisen, B., Hogan, J., Hohlfeld, M., Holzbauer, J. L., Hong, Z., Hopkins, W., Hou, S., Howley, I., Hubacek, Z., Hughes, R. E., Husemann, U., Hussein, M., Huston, J., Hynek, V., Iashvili, I., Ilchenko, Y., Illingworth, R., Introzzi, G., Iori, M., Ito, A. S., Ivanov, A., Jabeen, S., Jaffre, M., James, E., Jang, D., Jayasinghe, A., Jayatilaka, B., Jeon, E. J., Jeong, M. S., Jesik, R., Jiang, P., Jindariani, S., Johns, K., Johnson, E. E., Johnson, M., Jonckheere, A. A., Jones, M., Jonsson, P., Joo, K. K., Joshi, J., Jun, S. Y., Jung, A. W., Junk, T. R., Juste, A., Kajfasz, E., Kambeitz, M., Kamon, T., Karchin, P. E., Karmanov, D., Kasmi, A., Kato, Y., Katsanos, I., Kaur, M., Kehoe, R., Kermiche, S., Ketchum, W., Keung, J., Khalatyan, N., Khanov, A., Kharchilava, A., Kharzheev, Y. N., Kilminster, B., Kim, D. H., Kim, H. S., Kim, J. E., Kim, M. J., Kim, S. H., Kim, S. B., Kim, Y. J., Kim, Y. K., Kimura, N., Kirby, M., Kiselevich, I., Kohli, J. M., Kondo, K., Kong, D. J., Konigsberg, J. J., Kotwa, A. V., Kozelov, A. V., Kraus, J., Kreps, M., Kroll, J., Kruse, M., Kuhr, T., Kumar, A., Kupco, A., Kurata, M., Kurca, T., Kuzmin, V. A., Laasanen, A. T., Lammel, S., Lammers, S., Lancaster, M., Lannon, K., Latino, G., Lebrun, P., Lee, H. S., Lee, J. S., Lee, S. W., Lee, W. M., Lei, X., Lellouch, J., Leo, S., Leone, S., Lewis, J. D., Li, D., Li, H., Li, L., Li, Q. Z., Lim, J. K., Limosani, A., Lincoln, D., Linnemann, J., Lipaev, V. V., Lipeles, E., Lipton, R., Lister, A., Liu, H., Liu, Q., Liu, T., Liu, Y., Lobodenko, A., Lockwitz, S., Loginov, A., Lokajicek, M., de Sa, R. Lopes, Lucchesi, D., Luca, A., Lueck, J., Lujan, P., Lukens, P., Luna-Garcia, R., Lungu, G., Lyon, A. L., Lys, J., Lysak, R., Maciel, A. K. A., Madar, R., Madrak, R., Maestro, P., Magana-Villalba, R., Malik, S., Malyshev, V. L., Manca, G., Manousakis-Katsikakis, A., Mansour, J., Marchese, L., Margaroli, F., Marino, P., Martinez-Ortega, J., Matera, K., Mattson, M. E., Mazzacane, A., Mazzanti, P., McCarthy, R., McGivern, C. L., McNulty, R., Mehta, A., Mehtala, P., Meijer, M. M., Melnitchouk, A., Menezes, D., Mercadante, P. G., Merkin, M., Mesropian, C., Meyer, A., Meyer, J., Miao, T., Miconi, F., Mietlicki, D., Mitra, A., Miyake, H., Moed, S., Moggi, N., Mondal, N. K., Moon, C. S., Moore, R., Morello, M. J., Mukherjee, A., Mulhearn, M., Muller, Th., Murat, P., Mussini, M., Nachtman, J., Nagai, Y., Naganoma, J., Nagy, E., Nakano, I., Napier, A., Narain, M., Nayyar, R., Neal, H. A., Negret, J. P., Nett, J., Neustroev, P., Nguyen, H. T., Nigmanov, T., Nodulman, L., Noh, S. Y., Nomiella, O., Nunnemann, T., Oakes, L., Oh, S. H., Oh, Y. D., Okusawa, T., Orava, R., Orduna, J., Ortolan, L., Osman, N., Pagliarone, C., Pal, A., Palencia, E., Palni, P., Papadimitriou, V., Parashar, N., Parihar, V., Park, S. K., Parker, W., Partridge, R., Parua, N., Patwa, A., Pauletta, G., Paulini, M., Paus, C., Penning, B., Perfilov, M., Peters, Y., Petridis, K., Petrillo, G., Petroff, P., Phillips, T. J., Piacentino, G., Pianori, E., Pilot, J., Pitts, K., Plager, C., Pleier, M. -A, Podstavkov, V. M., Pondrom, L., Popov, A. V., Poprocki, S. S., Potamianos, K., Pranko, A., Prewitt, M., Price, D., Prokopenko, N., Prokoshin, F., Ptohos, F., Punzi, G., Qian, J., Quadt, A., Quinn, B., Ratoff, P. N., Razumov, I., Fernandez, I. Redondo, Renton, P., Rescigno, M., Rimondi, F., Ripp-Baudot, I., Ristori, L., Rizatdinova, F., Robson, A., Rodriguez, T., Rolli, S., Rominsky, M., Ronzani, M., Roser, R., Rosner, J. L., Ross, A., Royon, C., Rubinov, P., Ruchti, R., Ruffini, F., Ruiz, A., Russ, J., Rusu, V. V., Sajot, G., Sakumoto, W. K., Sakurai, Y., Sanchez-Hernandez, A., Sanders, M. M. P., Santi, L., Santos, A. S., Sato, K., Savage, C., Saveliev, V., Savitskyi, M., Savoy-Navarro, A., Sawyer, L., Scanlon, T., Schamberger, R. D., Scheglov, Y., Schellman, H., Schlabach, P., Schmidt, E. E., Schott, M., Schwanenberger, C., Schwarz, T., Schwienhorst, R., Scodellaro, L., Scuri, F., Seidel, S., Seiya, Y., Sekaric, J., Semenov, A., Severini, H., Sforza, F., Shabalina, E., Shalhout, S. Z., Sham, V., Shaw, S., Shchukin, A. A., Shears, T., Shepard, P. F., Shimojima, M., Shkola, O., Shochet, M., Shreyber-Tecker, I., Simak, V., Simonenko, A., Skubic, P., Slattery, P., Sliwa, K., Smith, J. R., Snider, F. D., Snow, C. R., Snow, J., Snyder, S., Soldner-Rembold, S., Song, H., Sonnenschein, L., Sorin, V., Soustruznik, K., St Denis, R., Stancari, M., Stark, J., Stetaniuk, N., Stentz, D., Stoyanova, D. A., Strauss, M., Strologas, J., Sudo, Y., Sukhanov, A., Suslov, I., Suter, L., Svoisky, P., Takemasa, K., Takeuchi, Y., Tang, J., Tecchio, M., Teng, P. K., Thom, J., Thomson, E., Thukral, V., Titov, M., Toback, D., Tokar, S., Tokmenin, V. V., Tollefson, K., Tomura, T., Tonelli, D., Torre, S., Torretta, D., Totaro, P., Trovato, M., Tsai, Y. -T, Tsybychev, D., Tuchming, B., Tully, C., Ukegawa, F. F., Uozumi, S., Uvarov, L., Uvarov, S., Uzunyan, S., Van Kooten, R., van Leeuwen, W. M., Varelas, N., Varnes, E. W., Vasilyev, I. A., Vazquez, F., Velev, G., Vellidis, C., Verkheev, A. Y., Vernieri, C., Vertogradov, L. S., Verzocchi, M., Vesterinen, M., Vidal, M., Vilanova, D., Vilar, R., Vizan, J., Vogel, M., Vokac, P., Volpi, G., Wagner, P., Wahl, H. D., Wallny, R., Wang, C., Wang, M. H. L. S., Wang, S. M., Warchol, J., Waters, D., Watts, G., Wayne, M., Weichert, J., Welty-Rieger, L., Wester, W. C. , I I I, Whiteson, D., Wicklund, A. B., Wilbur, S., Williams, H. H., Williams, M. R. J., Wilson, G. W., Wilson, J. S., Wilson, P., Winer, B. L., Wittich, P., Wobisch, M., Wolbers, S., Wolfmeister, H., Wood, D. R., Wright, T., Wu, X., Wu, Z., Wyatt, T. R., Xiang, Y., Xie, Y., Yamada, R., Yamamoto, K., Yamato, D., Yang, S., Yang, T., Yang, U. K., Yang, Y. C., Yao, W. -M, Yasuda, T., Yatsunenko, Y. A., Ye, W., Ye, Z., Yeh, C. P., Yi, K., Yin, H., Yip, K., Yoh, J., Yorita, K., Yoshida, T., Youn, S. W., Yu, G. B., Yu, I., Yu, J. J. M., Zanetti, A. M., Zeng, Y., Zennamo, J., Zhao, T. G., Zhou, B., Zhou, C., Zhu, J., Zielinski, M., Zieminska, D., Zivkovic, L., and Zucchelli, S.

Abstract

Drell-Yan lepton pairs produced in the process p (p) over bar -> l(+)l(-) + X through an intermediate gamma*/Z boson have an asymmetry in their angular distribution related to the spontaneous symmetry breaking of the electroweak force and the associated mixing of its neutral gauge bosons. The CDF and D0 experiments have measured the effective-leptonic electroweak mixing parameter sin(2) theta(lept)(eff) using electron and muon pairs selected from the full Tevatron proton-antiproton data sets collected in 2001-2011, corresponding to 9-10 fb(-1) of integrated luminosity. The combination of these measurements yields the most precise result from hadron colliders, sin(2)theta(lept)(eff) = 0.23148 +/- 0.00033. This result is consistent with, and approaches in precision, the best measurements from electron-positron colliders. The standard model inference of the on-shell electroweak mixing parameter sin(2) theta(W), or equivalently the W-boson mass M-W, using the ZFITTER software package yields sin(2) theta(W) = 0.22324 +/- 0.00033 or equivalently, M-W = 80.367 +/- 0.017 GeV/c(2).

Published
2018
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21. MiR-223-3p promotes the growth and invasion of neuroblastoma cell via targeting FOXO1.

Author

HAN, L. L., ZHOU, X. J., LI, F. J., HAO, X. W., JIANG, Z., DONG, Q., and CHEN, X.

Abstract

OBJECTIVE: MicroRNAs (miRNAs) have been demonstrated to have crucial roles in cancer development. We investigated the involvement of miR-223-3p in neuroblastoma (NB). MATERIALS AND METHODS: MiR-223-3p expression in NB cell lines and normal cell line was analyzed with real-time quantitative PCR method. Cell proliferation, cell invasion, and cell apoptosis were assessed by cell counting kit-8 (CCK-8), transwell invasion assay, and flow cytometry assay, respectively. Bioinformatics analysis, Dual-Luciferase reporter assays, and Western blot analysis were conducted to identify the connection of miR-223-3p and forkhead box O1 (FOXO1). RESULTS: MiR-223-3p level was found highly expressed in NB cell lines compared with normal cell line. Knockdown miR-223-3p expression decreased cell growth and invasion but increased cell apoptosis. MiR-223-3p was able to bind with the 3'-untranslated region of FOXO1, and thereby resulting in a reduction of FOXO1 expression. The knockdown of FOXO1 increased the malignant capacity of NB cells. CONCLUSIONS: Therefore, given the fact that miR-223-3p suppressed FOXO1 expression to promote NB progression, targeting miR-223-3p may be an effective method for NB treatment. [ABSTRACT FROM AUTHOR]

Published
2019

22. Detection of magnetic circular dichroism in amorphous materials utilizing a single-crystalline overlayer

Author

Lin, J., Zhong, X. Y., Song, C., Rusz, Jan, Kocevski, Vancho, Xin, H. L., Cui, B., Han, L. L., Lin, R. Q., Chen, X. F., Zhu, J., Lin, J., Zhong, X. Y., Song, C., Rusz, Jan, Kocevski, Vancho, Xin, H. L., Cui, B., Han, L. L., Lin, R. Q., Chen, X. F., and Zhu, J.

Abstract

Electron energy-loss magnetic chiral dichroism (EMCD) is a novel technique that allows magnetic information determination down to the nanoscale. However, constrained by the predefined diffraction geometry in regular EMCD experiments, it has not yet been feasible to obtain EMCD signals from amorphous materials, due to the lack of long-range ordering. Here we propose a protocol for EMCD detection in amorphous materials utilizing a single-crystalline overlayer acting as a two-beam splitter. Phase locking of the EMCD signals is observed and explained by two conceivable scenarios. Both experimental results and theoretical calculations demonstrate significant EMCD signals of amorphous materials.

Published
2017
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23. Genetic and functional diversity of ubiquitous DNA viruses in selected Chinese agricultural soils

Author

Han, L-L, Yu, D-T, Zhang, L-M, Shen, J-P, He, J-Z, Han, L-L, Yu, D-T, Zhang, L-M, Shen, J-P, and He, J-Z

Abstract

Viral community structures in complex agricultural soils are largely unknown. Electron microscopy and viromic analyses were conducted on six typical Chinese agricultural soil samples. Tailed bacteriophages, spherical and filamentous viral particles were identified by the morphological analysis. Based on the metagenomic analysis, single-stranded DNA viruses represented the largest viral component in most of the soil habitats, while the double-stranded DNA viruses belonging to the Caudovirales order were predominanted in Jiangxi-maize soils. The majority of functional genes belonged to the subsystem "phages, prophages, transposable elements, and plasmids". Non-metric multidimensional analysis of viral community showed that the environment medium type was the most important driving factor for the viral community structure. For the major viral groups detected in all samples (Microviridae and Caudovirales), the two groups gathered viruses from different sites and similar genetic composition, indicating that viral diversity was high on a local point but relatively limited on a global scale. This is a novel report of viral diversity in Chinese agricultural soils, and the abundance, taxonomic, and functional diversity of viruses that were observed in different types of soils will aid future soil virome studies and enhance our understanding of the ecological functions of soil viruses.

Published
2017

24. Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Author

Wang, Q, Zhang, L-M, Shen, J-P, Du, S, Han, L-L, He, J-Z, Wang, Q, Zhang, L-M, Shen, J-P, Du, S, Han, L-L, and He, J-Z

Abstract

Ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) are crucial for N2O emission as they carry out the key step of nitrification. Dicyandiamide (DCD) and acetylene (C2H2) are typical nitrification inhibitors (NIs), while the comparative effects of these NIs on N2O production and ammonia oxidizers' (AOB and AOA) growth are unclear. Four treatments including a control, urea, urea + DCD, and urea + C2H2 were set up to investigate their effect of inhibiting soil nitrification, nitrification-related N2O emission as well as the growth of ammonia oxidizers with a fluvo-aquic soil using microcosms for 28 days. N2O emission and net nitrification rate increased after the application of urea, but were significantly restrained in urea + NI treatments, while C2H2 was more effective in reducing N2O emission and nitrification rate than DCD. The abundance of AOB, which was significantly correlated with N2O emission and net nitrification rate, was more inhibited by C2H2 than DCD. Furthermore, the application of urea in all the soils had little impact on the AOA community, while obvious shifts of AOB community structure were found compared with the control. All AOB sequences fell within Nitrosospira cluster 3, and the AOA community was clustered to group 1.1b. Collectively, it indicated that application of urea combined with NIs (DCD or C2H2) could potentially alter N2O emission, mainly through regulating the growth of AOB but not AOA in this fluvo-aquic soil.

Published
2016

25. Primary Succession of Nitrogen Cycling Microbial Communities Along the Deglaciated Forelands of Tianshan Mountain, China

Author

Zeng, J, Lou, K, Zhang, C-J, Wang, J-T, Hu, H-W, Shen, J-P, Zhang, L-M, Han, L-L, Zhang, T, Lin, Q, Chalk, PM, He, J-Z, Zeng, J, Lou, K, Zhang, C-J, Wang, J-T, Hu, H-W, Shen, J-P, Zhang, L-M, Han, L-L, Zhang, T, Lin, Q, Chalk, PM, and He, J-Z

Abstract

Structural succession and its driving factors for nitrogen (N) cycling microbial communities during the early stages of soil development (0-44 years) were studied along a chronosequence in the glacial forelands of the Tianshan Mountain No.1 glacier in the arid and semi-arid region of central Asia. We assessed the abundance and population of functional genes affiliated with N-fixation (nifH), nitrification (bacterial and archaeal amoA), and denitrification (nirK/S and nosZ) in a glacier foreland using molecular methods. The abundance of functional genes significantly increased with soil development. N cycling community compositions were also significantly shifted within 44 years and were structured by successional age. Cyanobacterial nifH gene sequences were the most dominant N fixing bacteria and its relative abundance increased from 56.8-93.2% along the chronosequence. Ammonia-oxidizing communities shifted from the Nitrososphaera cluster (AOA-amoA) and the Nitrosospira cluster ME (AOB-aomA) in younger soils (0 and 5 years) to communities dominated by soil and sediment 1 (AOA-amoA) and Nitrosospira Cluster 2 Related (AOB-aomA) in older soils (≥17 years). Most of the denitrifers closest relatives were potential aerobic denitrifying bacteria, and some other types of denitrifying bacteria (like autotrophic nitrate-reducing, sulfide-oxidizing bacteria and denitrifying phosphorus removing bacteria) were also detected in all soil samples. The regression analysis showed that N cycling microbial communities were dominant in younger soils (0-5 years) and significantly correlated with soil total carbon, while communities that were most abundant in older soils were significantly correlated with soil total nitrogen. These results suggested that the shift of soil C and N contents during the glacial retreat significantly influenced the abundance, composition and diversity of N cycling microbial communities.

Published
2016

29. P0809 : 98% SVR12 in Korean And Taiwanese patients with chronic genotype 2 HCV infection receiving 12 weeks of sofosbuvir plus ribavirin: Results from an international, multicenter phase 3 study

Author

Kao, J.-H., primary, Ahn, S.-H., additional, Chien, R.-N., additional, Jeong, S.-H., additional, Peng, C.-Y., additional, Lim, Y.-S., additional, Yang, J.C., additional, Mo, H., additional, Han, L.-L., additional, Brainard, D., additional, Knox, S.J., additional, McHutchison, J.G., additional, Lee, Y.-J., additional, Chang, T.-T., additional, Paik, S.-W., additional, Chu, C.-J., additional, Chuang, W.-L., additional, and Han, K.-H., additional

Published
2015
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32. A double-blind, randomized, prospective, phase II trial of capecitabine (Cap) plus enzastaurin (Enz) versus cap plus placebo in patients (pts) with advanced breast cancer (BrCa) and prior anthracycline and taxane treatment.

Author

Clemons, M., primary, Gelmon, K. A., additional, Lynch, J., additional, Kotliar, M. L., additional, Joy, A., additional, Jordaan, J. P., additional, Han, L. L., additional, Blair, J. M., additional, and Iscoe, N. A., additional

Published
2010
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40. Preliminary Study on the Physiological Characteristics of Transgenic Wheat with Maize C4-pepcGene in Field Conditions

Author

Han, L. L., Xu, W. G., Hu, L., Li, Y., Qi, X. L., Zhang, J. H., Zhang, H. F., and Wang, Y. X.

Abstract

To explore the physiological characteristics of the pepcgene in transgenic wheat (Triticum aestivum) plants, PEPC activities in various organs of T3 plants were analyzed at Feekes 6.0, Feekes 10.3 and Feekes 11.1, and compared to control, untransformed wheat cultivar Zhoumai 19. Net photosynthetic rates (Pn) in leaves were also measured at the same stages. At Feekes 11.1, both transgenic and control plants were treated with DCDP. Yield traits were surveyed after harvest. The results indicated that Pnand PEPC activity in the flag leaf of transgenic wheat were significantly higher than those of the control at different stages. At Feekes 10.3, Pnreached the highest value at 28.2 µmol m-2s-1and PEPC activity reached the highest value at 104.6 µmol h-1mg-1. Both factors significantly increased by 21% compared to the control at Feekes 11.1. PEPC activity in the flag leaf of transgenic plants was significantly higher than that of non-leaf organs. Pnof transgenic plants was greatly reduced after DCDP treatment. In the flag leaf of transgenic wheat, Pnwas significantly correlated to PEPC activities at 0.01 probability level with a correlation coefficient of 0.8957**. The yield traits of transgenic line 1-27-3, such as 1000-grain weight, single spike weight and harvest index were higher than those of the control. Additionally, the spike weight of 1-27-3 showed an increase of approximately 9.5% compared to the control. These results indicated that the expression of maize (Zea mays) pepcgene was different across various organs of transgenic wheat and across every growth stage. Therefore, we conclude that introducing maize pepc gene into wheat plants can increase their Pnand improve production.

Published
2014
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47. [Correlation analysis between myopia growth and physical development parameters of school-age children in Shihezi area, Xinjiang Uyghur Autonomous Region].

Author

Bai XQ, Zhang CY, Du R, Ren YY, Li ZS, Yan HG, Wang Z, Zhang XH, Quan XJ, Li J, Han LL, Xu X, Zhou Y, Liu MB, Zhang HY, and Li L

Subjects
Humans, Child, Male, Female, Adolescent, Cross-Sectional Studies, China, Body Mass Index, Students, Biometry, Myopia
Abstract

Objective: To study the correlation between myopia growth and physical development of primary school student in Shihezi area of Xinjiang Uyghur Autonomous Region by measuring the ocular biometry and physical parameters of the eye, and to provide an objective basis for the development of myopia prevention and control. Methods: A cross-sectional study design was used. The Grade 1-6 students from 33 schools in Shihezi area were randomly selected in October 2023. The children were classified according to age into the low age group (6-9 years) and the high age group (10-13 years).The children's height, weight, axial length (AL), maximum keratometry (steepest keratometry, K1), and minimum keratometry (flattest keratometry, K1) were measured. The body mass index (BMI), keratometric astigmatism (ΔK), mean keratometry (K), corneal radius (CR) and AL/CR were calculated. Correlation analyses were performed between ocular biometry and physical parameters. LASSO regression analysis was used to screen the best variables, and linear regression models were constructed after adjusting for confounding factors of age and sex. Results: A total of 3 986 children aged 6-13 years were included in this study, including 2 094 boys (52.53%) and 1 892 girls (47.47%); 2 162 in the lower age group and 1 824 in the upper age group. Girls had higher K, ΔK and AL/CR values than boys (43.38 D vs 42.7 D, t =-7.84, P <0.01; 1.1 D vs 1.01 D, t =-2.14, P =0.03; 2.97 vs 2.96, t =3.32, P =0.02). Boys had higher AL, height, weight and BMI than girls (23.47 mm vs 23.02 mm, t =13.77, P <0.01; 1.34 m vs 1.33 m, t =3.02, P =0.03; 32 kg vs 29.5 kg, t =7.82, P <0.01; 17.4 vs 16.52, t =10.36, P <0.01). LASSO regression was used to screen the 3 physical parameter variables (height, weight, and BMI) to determine that height was the best physical parameter variable for AL/CR. After adjusting for confounders, it was shown that in the low age group (6-9 years), there was a significant positive correlation between children's height and AL/CR ( b =0.174, 95% CI : 0.106 to 0.241, P <0.001), whereas in the high age group (10-13 years), there was no correlation between children's height and AL/CR ( b =0.086, 95% CI :-0.038 to 0.21, P =0.174). Conclusion: There was a positive correlation between height and myopia growth in the lower age group of children in Shihezi area, Xinjiang Uyghur Autonomous Region; there was no correlation between height and myopia growth in the higher age group.

Published
2024
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48. Microbial species pool-mediated diazotrophic community assembly in crop microbiomes during plant development.

Author

Xiong C, K Singh B, Zhu Y-G, Hu H-W, Li P-P, Han Y-L, Han L-L, Zhang Q-B, Wang J-T, Liu S-Y, Wu C-F, Ge A-H, Zhang L-M, and He J-Z

Subjects
Agriculture, Soil chemistry, Nitrogen analysis, Crops, Agricultural metabolism, Plant Development, Microbiota genetics
Abstract

Plant-associated diazotrophs strongly relate to plant nitrogen (N) supply and growth. However, our knowledge of diazotrophic community assembly and microbial N metabolism in plant microbiomes is largely limited. Here we examined the assembly and temporal dynamics of diazotrophic communities across multiple compartments (soils, epiphytic and endophytic niches of root and leaf, and grain) of three cereal crops (maize, wheat, and barley) and identified the potential N-cycling pathways in phylloplane microbiomes. Our results demonstrated that the microbial species pool, influenced by site-specific environmental factors (e.g., edaphic factors), had a stronger effect than host selection (i.e., plant species and developmental stage) in shaping diazotrophic communities across the soil-plant continuum. Crop diazotrophic communities were dominated by a few taxa (~0.7% of diazotrophic phylotypes) which were mainly affiliated with Methylobacterium , Azospirillum , Bradyrhizobium , and Rhizobium . Furthermore, eight dominant taxa belonging to Azospirillum and Methylobacterium were identified as keystone diazotrophic taxa for three crops and were potentially associated with microbial network stability and crop yields. Metagenomic binning recovered 58 metagenome-assembled genomes (MAGs) from the phylloplane, and the majority of them were identified as novel species (37 MAGs) and harbored genes potentially related to multiple N metabolism processes (e.g., nitrate reduction). Notably, for the first time, a high-quality MAG harboring genes involved in the complete denitrification process was recovered in the phylloplane and showed high identity to Pseudomonas mendocina . Overall, these findings significantly expand our understanding of ecological drivers of crop diazotrophs and provide new insights into the potential microbial N metabolism in the phyllosphere.IMPORTANCEPlants harbor diverse nitrogen-fixing microorganisms (i.e., diazotrophic communities) in both belowground and aboveground tissues, which play a vital role in plant nitrogen supply and growth promotion. Understanding the assembly and temporal dynamics of crop diazotrophic communities is a prerequisite for harnessing them to promote plant growth. In this study, we show that the site-specific microbial species pool largely shapes the structure of diazotrophic communities in the leaves and roots of three cereal crops. We further identify keystone diazotrophic taxa in crop microbiomes and characterize potential microbial N metabolism pathways in the phyllosphere, which provides essential information for developing microbiome-based tools in future sustainable agricultural production., Competing Interests: The authors declare no conflict of interest.

Published
2024
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49. [Clinical characteristics of 272 437 patients with different histopathological subtypes of primary esophageal malignant tumors].

Author

Wang LD, Li X, Song XK, Zhao FY, Zhou RH, Xu ZC, Liu AL, Li JL, Li XZ, Wang LG, Zhang FH, Zhu XM, Li WX, Zhao GZ, Guo WW, Gao XM, Li LX, Wan JW, Ku QX, Xu FG, Zhu AF, Ji HX, Li YL, Ren SL, Zhou PN, Chen QD, Bao SG, Gao HJ, Yang JC, Wei WM, Mao ZZ, Han ZW, Chang YF, Zhou XN, Han WL, Han LL, Lei ZM, Fan R, Wang YZ, Yang JJ, Ji Y, Chen ZJ, Li YF, Hu L, Sun YJ, Chen GL, Bai D, and You D

Subjects
Female, Humans, Male, Carcinoma, Small Cell, Esophageal Neoplasms pathology, Esophageal Squamous Cell Carcinoma pathology, Histiocytoma, Malignant Fibrous, Melanoma
Abstract

Objective: To characterize the histopathological subtypes and their clinicopathological parameters of gender and onset age by common, rare and sparse primary esophageal malignant tumors (PEMT). Methods: A total of 272 437 patients with PEMT were enrolled in this study, and all of the patients were received radical surgery. The clinicopathological information of the patients was obtained from the database established by the State Key Laboratory of Esophageal Cancer Prevention & Treatment from September 1973 to December 2020, which included the clinical treatment, pathological diagnosis and follow-up information of esophagus and gastric cardia cancers. All patients were diagnosed and classified by the criteria of esophageal tumor histopathological diagnosis and classification (2019) of the World Health Organization (WHO). The esophageal tumors, which were not included in the WHO classification, were analyzed separately according to the postoperative pathological diagnosis. The χ 2 test was performed by the SPSS 25.0 software on count data, and the test standard α=0.05. Results: A total of 32 histopathological types were identified in the enrolled PEMT patients, of which 10 subtypes were not included in the WHO classification. According to the frequency, PEMT were divided into common (esophageal squamous cell carcinoma, ESCC, accounting for 97.1%), rare (esophageal adenocarcinoma, EAC, accounting for 2.3%) and sparse (mainly esophageal small cell carcinoma, malignant melanoma, etc., accounting for 0.6%). All the common, rare, and sparse types occurred predominantly in male patients, and the gender difference of rare type was most significant (EAC, male∶ female, 2.67∶1), followed with common type (ESCC, male∶ female, 1.78∶1) and sparse type (male∶ female, 1.71∶1). The common type (ESCC) mainly occurred in the middle thoracic segment (65.2%), while the rare type (EAC) mainly occurred in the lower thoracic segment (56.8%). Among the sparse type, malignant melanoma and malignant fibrous histiocytoma were both predominantly located in the lower thoracic segment (51.7%, 66.7%), and the others were mainly in the middle thoracic segment. Conclusion: ESCC is the most common type among the 32 histopathological types of PEMT, followed by EAC as the rare type, and esophageal small cell carcinoma and malignant melanoma as the major sparse type, and all of which are mainly occur in male patients. The common type of ESCC mainly occur in the middle thoracic segment, while the rare type of EAC mainly in the lower thoracic segment. The mainly sparse type of malignant melanoma and malignant fibrous histiocytoma predominately occur in the lower thoracic segment, and the remaining sparse types mainly occur in the middle thoracic segment.

Published
2022
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50. [Association of fibroblast growth factor 23 with age-related cardiac diastolic function subclinical state in a healthy Chinese population].

Author

Huo YY, Bai XJ, Han LL, Wang N, Han W, and Sun XF

Subjects
Adult, Aged, Aged, 80 and over, China, Diastole, Female, Fibroblast Growth Factor-23, Fibroblast Growth Factors, Humans, Male, Middle Aged, Ventricular Dysfunction, Left
Abstract

Objective: To determine the association of serum fibroblast growth factor-23 concentrations with age-related cardiac diastolic function subclinical state and whether this association differs by sex. Methods: Seven hundred sixteen healthy subjects (aged 35-89 years, 68.4% female) were selected from National Basic Research Program of China (973 Program-China Medical University subsection) between January 2014 and February 2015 and assigned into 4 groups according to sex and age:< 60 years old male and female group, ≥ 60 years old male and female group. Blood biochemical indicators and general clinical data of the subjects were measured. The glomerular filtration rate (eGFR) were estimated using the Modified Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI-ASIA) equation. The fibroblast factor 23 (FGF-23), C-reactive protein (CRP) and interleukin-6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). Cardiac structure and function parameters including left atrial diameter (LAD), inter-ventricular septum thickness (IVST), left ventricle posterior wall thickness (LVPWT), left ventricle mass index (LVMI),left atrial mass index (LAVI) and the ratio of peak velocity of early filling to the septal early peak diastolic mitral annulus velocity(E/e') were measured by echocardiography. Association between serum FGF-23 and aging-related diastolic function subclinical status was analyzed by binary Logistic regression analysis. Results: (1) Serum log-transformed FGF-23 levels were significantly higher in males than in females [(2.0±0.3) ng/L vs (1.9±0.4) ng/L, P< 0.05]. (2) Cardiac diastolic function gradually decreased with age, and age related cardiac diastolic function decline of female was significantly higher than males[E/e':<60 years old male group (7.6±2.6), ≥ 60 years old male group (8.6±2.7), P< 0.01;<60 years old female group (8.3±2.3), ≥ 60 years old female group (9.5±3.1), P< 0.01; LAVI:<60 years old female group (14±5) ml/m(2), ≥ 60 years old female group (16±5) ml/m(2), P< 0.01]. (3) Serum FGF-23 was significantly positively correlated with age ( r= 0.089, P< 0.05) and LAVI ( r= 0.084, P< 0.05) in total study population while with E/e' ( r= 0.149, P< 0.05) only in males. There was no significant correlation between serum FGF-23 and cardiac diastolic function parameters in females. (4) Binary Logistic regression analysis showed that median and high FGF-23 were independently associated with age-related cardiac diastolic function decline ( OR= 2.831, 95% CI: 1.144-7.009, P= 0.024; OR= 2.548, 95% CI: 1.053-6.163, P= 0.038) in males. Conclusions: Serum FGF-23 concentrations are associated with age-related cardiac diastolic function subclinical state in a healthy Chinese population. High levels of FGF-23 are independently associated with age-related cardiac diastolic function decline in males.

Published
2019
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