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A Gram-positive, facultative anaerobic, rod-shaped, and endospore-forming strain, designated 53-2T was isolated from the root nodule of Oxytropis ochrocephala Bunge growing on Qilian mountain, China. The strain can grow at pH 7.0-8.0, 10-50 °C and tolerate up to 11% NaCl. Optimal growth occurred at pH 7.2 and 37 °C. The result of BLASTn search based on 16S rRNA gene sequence revealed that strain 53-2T, being closest related to Bacillus acidicola 105-2T, possessed remote similarity (less than 95.64%) to the species within genus Bacillus. The DNA G + C content was 37.8%. Chemotaxonomic data (major quinone is MK-7; major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, unknown phospholipid, and aminoglycophospholipid; fatty acids are anteiso-C15: 0, iso-C15:0 and anteiso-C17: 0) supported the affiliation of the isolate to the genus Bacillus. On the basis of physiological, phylogenetic, and biochemical properties, strain 53-2T represents a novel species within genus Bacillus, for which the name Bacillus radicibacter is proposed. The type strain is 53-2T (=DSM27302T=ACCC06115T=CCNWQLS5T). [ABSTRACT FROM AUTHOR]