Search

Wound healing is a physiological process occurring after the onset of a skin lesion aiming to reconstruct the dermal barrier between the external environment and the body. Depending on the nature and duration of the healing process, wounds are classified as acute (e.g., trauma, surgical wounds) and chronic (e.g., diabetic ulcers) wounds. The latter, often affect millions of people globally, take months to heal or not heal non-healing chronic wounds, are typically susceptible to microbial infection, and are a major cause of morbidity. Wounds can be treated with a variety of non-surgical (topical formulations, wound dressings) and surgical (debridement, skin grafts/flaps) methods. Three-dimensional (3D)-(bio) printing and traditional wound dressings are two examples of modern experimental techniques. This review focuses on several types of anti-inflammatory wound dressings, especially focusing on hydrogels and traditional macro-fungi like L. rhinocerotis as agents that promote wound healing. In this study, we introduced novel anti-inflammatory hydrogel dressings and offered innovative methods for application and preparation to aid in the healing. Additionally, we summarize the key elements required for wound healing and discuss our analysis of potential future issues. These findings suggest that L. rhinocerotis and various anti-inflammatory hydrogels can be considered as conventional and alternative macro-fungi for the treatment of non-communicable diseases. We summarized the development of functional hydrogel dressings and traditional Lignosus rhinoceros used for wound healing agents in recent years, as well as the current situation and future trends, in light of their preparation mechanisms and functional effects.

Extracellular vesicles (EVs) have emerged as key intercellular communication and pathogenesis mediators. Parasitic organisms’ helminths, cause widespread infections with significant health impacts worldwide. Recent research has shed light on the role of EVs in the lifecycle, immune evasion, and disease progression of these parasitic organisms. These tiny membrane-bound organelles including microvesicles and exosomes, facilitate the transfer of proteins, lipids, mRNAs, and microRNAs between cells. EVs have been isolated from various bodily fluids, offering a potential diagnostic and therapeutic avenue for combating infectious agents. According to recent research, EVs from helminths hold great promise in the diagnosis of parasitic infections due to their specificity, early detection capabilities, accessibility, and the potential for staging and monitoring infections, promote intercellular communication, and are a viable therapeutic tool for the treatment of infectious agents. Exploring host-parasite interactions has identified promising new targets for diagnostic, therapy, and vaccine development against helminths. This literature review delves into EVS’s origin, nature, biogenesis, and composition in these parasitic organisms. It also highlights the proteins and miRNAs involved in EV release, providing a comprehensive summary of the latest findings on the significance of EVs in the biology of helminths, promising targets for therapeutic and diagnostic biomarkers.

Millions of microorganisms make up the complex microbial ecosystem found in the human gut. The immune system’s interaction with the gut microbiota is essential for preventing inflammation and maintaining intestinal homeostasis. Numerous metabolic products that can cross-talk between immune cells and the gut epithelium are metabolized by the gut microbiota. Traumatic injury elicits a great and multifaceted immune response in the minutes after the initial offense, containing simultaneous pro- and anti-inflammatory responses. The development of innovative therapies that improve patient outcomes depends on the gut microbiota and immunological responses to trauma. The altered makeup of gut microbes, or gut dysbiosis, can also dysregulate immunological responses, resulting in inflammation. Major human diseases may become more common as a result of chronic dysbiosis and the translocation of bacteria and the products of their metabolism beyond the mucosal barrier. In this review, we briefly summarize the interactions between the gut microbiota and the immune system and human disease and their therapeutic probiotic formulations. We also discuss the immune response to traumatic injury.

Protozoa exert a serious global threat of growing concern to human, and animal, and there is a need for the advancement of novel therapeutic strategies to effectively treat or mitigate the impact of associated diseases. Omega polyunsaturated fatty acids (ω-PUFAs), including Omega-3 (ω-3) and omega-6 (ω-6), are constituents derived from various natural sources, have gained significant attention for their therapeutic role in parasitic infections and a variety of essential structural and regulatory functions in animals and humans. Both ω-3 and ω-6 decrease the growth and survival rate of parasites through metabolized anti-inflammatory mediators, such as lipoxins, resolvins, and protectins, and have both in vivo and in vitro protective effects against various protozoan infections. The ω-PUFAs have been shown to modulate the host immune response by a commonly known mechanism such as (inhibition of arachidonic acid (AA) metabolic process, production of anti-inflammatory mediators, modification of intracellular lipids, and activation of the nuclear receptor), and promotion of a shift towards a more effective immune defense against parasitic invaders by regulation the inflammation like prostaglandins, leukotrienes, thromboxane, are involved in controlling the inflammatory reaction. The immune modulation may involve reducing inflammation, enhancing phagocytosis, and suppressing parasitic virulence factors. The unique properties of ω-PUFAs could prevent protozoan infections, representing an important area of study. This review explores the clinical impact of ω-PUFAs against some protozoan infections, elucidating possible mechanisms of action and supportive therapy for preventing various parasitic infections in humans and animals, such as toxoplasmosis, malaria, coccidiosis, and chagas disease. ω-PUFAs show promise as a therapeutic approach for parasitic infections due to their direct anti-parasitic effects and their ability to modulate the host immune response. Additionally, we discuss current treatment options and suggest perspectives for future studies. This could potentially provide an alternative or supplementary treatment option for these complex global health problems.

No abstract available.

Introduction: Trigeminal neuralgia is a condition of craniofacial pain which is characterized by sudden, excruciating, and brief pain in the sensory distribution of one or more branches of the fifth cranial nerve resulting in decreased quality of life of the affected patient. In patient’s refractory to pharmacological or conservative treatment, the surgical procedure of choice is microvascular decompression. The aim of our study was to determine the outcome of retro mastoid suboccipital microvascular decompression technique for the trigeminal neuralgia. Material & Methods: This case series study was conducted in Department of Neurosurgery Prime Teaching Hospital and Irfan General Hospital Peshawar from January 2020 to January 2022. Consent from the ethical committee and patients were taken. All patients of trigeminal neuralgia were included. Those patients having mass lesion were excluded. All information were put in proforma. Results were analysed by SPSS version 20. Results: Total sample size of our study was 58 patients. Majority of the patients were female (n 34 59%). Mean age of the patients was 44+ 5 years (range 25-60 years). The common level of pain distribution was in maxillary and mandibular branches V2-V3, 39(67.8%). The most common aetiology of trigeminal neuralgia was superior cerebellar artery loop in 41(70.7%) patients. Surgical outcome in terms of pain relief was in 49 (84.5 %) patients, after 6-8 months follow-up, The most common complication of surgery was CSF leak in 3(5.2%) patients, followed by post operative Sub arachnoid haemorrhage in 1(1.8%) patient, mortality was reported in 2 (3.4%) patients. Conclusion: The results of our study concluded that microvascular decompression surgery is effective in reducing pain, improving patient satisfaction in trigeminal neuralgia patients who are refractory to conservative treatment options such as carbamazepine’s. The procedure also has some complications such as cerebrospinal fluid leakage and sub arachnoid haemorrhage.

Introduction: The cause of sciatica is not always discogenic and majority of patients are misdiagnosed as having discogenic sciatica while the underlying non discogenic causes are overlooked. This study aimed at evaluating the frequency of non-discogenic sciatica in patients presenting with lumber radiculopathy. Material & Methods: A prospective case series study was conducted from February 2022 to January 2023 at Ali Institute of Neurosciences, Irfan General Hospital Peshawar. Nonprobability convenience sampling technique was incorporated. A total of 624 patients were reported to the OPD with clinical presentations of sciatica out of which 81 patients were included after thorough subjective, objective, and radiological investigation. Data was analysed using SPSS version 26. Results: Five hundred forty-three (87%) patients were diagnosed with discogenic sciatica while 81(13%) patients were categorized under non discogenic disorders causing radicular symptoms. Majority of the patients 51(63%) were males as compared to females 30(37%) in the non-discogenic group. Out of 81 cases diagnosed with non-discogenic sciatica,26 cases (32%) were due to spinal tumours, facet dysfunction 10(12%), piriformis syndrome6(7%), cluneal nerve disorder2(2%), quadratus lumborum dysfunction4(5%), osteoarthritis of the hip3(4%), peripheral nerve disorders 8(10%), sacroiliac joint 3(4%) infections 10(13%),trauma to gluteal region 3(4%), pregnancy associated sciatica 2(2%), compressive neuropathy 4(5%). Conclusion: There are a number of non-discogenic disorders causing symptoms of lumber radiculopathy. Identifying the underlying non discogenic cause is still challenging. Considering the possible pathologies, detailed history taking and objective examination along with diagnostic tests and investigations can lead to the identification of underlying pathologies and their management.

Brucellosis is a major threat to public health especially in developing countries including Pakistan. This study reveals the characterisation of Brucella species affecting humans and goats in the Swat region of Khyber Pakhtunkhwa, Pakistan. Blood samples were collected from shepherds and goats and analysed by Rose Bengal precipitation test (RBPT), standard plate agglutination test (SPAT), polymerase chain reaction (PCR) and Sanger sequencing of 16S rRNA gene. The findings of the study indicated 24% (36/150) and 11.3% (17/150) positivity for Brucella abortus and Brucella melitensis, respectively, in human samples. In samples of goats, 26.66% (40/150) were positive for B. abortus and 16.66% (25/150) samples were positive B. melitensis by SPAT. The species-specific PCR confirmed B. abortus in 24% (36/150) of human samples and 26.66% (17/150) of goat samples by targeting the IS711 locus. The remaining seropositive samples were confirmed as B. melitensis using IS711 M species-specific primer. The sequences of the amplified fragments of the 16S rRNA gene were blasted, and phylogenetic analysis revealed that Brucella species circulating in the Swat district were closely related to B. melitensis and B. abortus reported from India, China, Philippines, and the United States (US) showing the existence of the possible epidemiological linkage among the Brucella species. This study concluded that there was a higher prevalence of B. abortus (26.6%) in humans and goats compared to B. melitensis (16.6%). These results revealed that the Brucella species were circulating in both humans and goats in the study areas. The findings of the study concluded that B. abortus and B. melitensis were circulating in goats and shepherds with a higher prevalence of B. abortus than B. melitensis. Furthermore, the Brucella species identified in Swat were phylogenetically related to the Brucella species reported from India, China, Philippines and the US. Contribution: The proposed study covers the scope of the journal. The species of the genus Brucella affect both animals and shepherds. This study investigates the seroprevalence of brucellosis in shepherds and goats in different geographical areas in the Swat district. The phylogenetic analysis of the Brucella spp. identified in Swat showed close relationships to the Brucella species reported in India, China, Philippines and the US, which shows the possible epidemiological linkages between the Brucella spp.

Over the last two decades, the rapid expansion of the Internet has prompted a growing number of enterprises to deploy their work globally. Companies are increasingly reliant on software systems, which need ongoing modification, maintenance, and upgrades. The maintenance phase consumes approximately 80% of the total software budget. Hence, companies have been eagerly looking for offshore outsourcing of these software systems. Choosing the best sourcing model for software maintenance projects remains elusive and challenging due to a variety of technological, social, and political factors. This study aims to analyze application maintenance offshoring related factors and addresses its decision-making process. To achieve the study objectives, factors of two datasets are analyzed based on standard deviation, mean and mean error. The Critical Success Factors (CSFs) are examined thoroughly to explore their impact on decision-making process. Additionally, the study proposes a sourcing framework based on CSFs that uses the Human Computer Interaction (HCI) principles. This framework assists clients and vendors to evaluate the projects prior to offshoring decisions. To enhance decision-making process, a case study is conducted in the Information Technology (IT) industry and the Simple Multi Attribute Rating Technique (SMART) is applied. As the results show, SMART ranks the available options and helps in making effective offshoring decisions.

Byline: G. Khola and U. Hanif ABSTRACT Soil samples collected from an archaeological site Gulabi Bagh Lahore to evaluate pollen morphology and type of pollen present in the surface soil [...]

Abstract Background Mastitis in dairy cattle is a highly prevalent infectious disease, caused by various pathogens, mainly Staphylococcu aureus and Escherichia Coli, considerable economic loss worldwide. Objectives The aim of this study was to evaluate the in vitro activity of Herbal plants used against S. aureus and E. coli bacteria which are the causative agents of mastitis. Methods Therefore, in this study we investigate the antimicrobial effect of plant to evaluate the in vitro antibacterial activity of squaw mint (Mentha pulegium L., Lamiaceae family), catnip (Nepeta cataria L., Lamiaceae), lemon balm (Melissa officinalis L., Lamiaceae), for mastitis treatment. Solutions prepared in fixed oils, against S. aureus and E. coli bacteria which are the main agents of mastitis. Isolation and antibiotic susceptibility analyses of milk samples taken from 100 subclinical mastitis dairy cows were performed. The antibacterial properties of the solutions were analysed by a disk diffusion method. Results In the bacterial isolation, S. aureus was determined 97.7% and E. coli 53.5% positive of cows with mastitis. Antibacterial susceptibility test of the Lemon balm extract and essential oil showed maximum zone of inhibition against S. aureus 30 µl (23 mm), followed by 20 µl (19 mm), E. coli (19 mm) and 10 µl (5–7 mm), of the same extract against the Gram‐positive bacteria. The ethanol extracts show the similar activity against the Gram‐negative bacteria at 30, 20, and 10 µl (18–20 mm). Followed by S. aureus, when the zone areas for the susceptible solutions (Lemon balm, and essential oil) and the control group were compared, determined that there was little difference between for S. aureus and E. coli. Conclusions This study hence indicated that in vitro cultured plantlets of lemon balm and peppermint oil can be used as the alternative method for production of mastitis and cheap source its precursor with antimicrobial activities.

The gut microbiota (GM) plays an important role in the physiology and pathology of the host. Microbiota communicate with different organs of the organism by synthesizing hormones and regulating body activity. The interaction of the central nervous system (CNS) and gut signaling pathways includes chemical, neural immune and endocrine routes. Alteration or dysbiosis in the gut microbiota leads to different gastrointestinal tract disorders that ultimately impact host physiology because of the abnormal microbial metabolites that stimulate and trigger different physiologic reactions in the host body. Intestinal dysbiosis leads to a change in the bidirectional relationship between the CNS and GM, which is linked to the pathogenesis of neurodevelopmental and neurological disorders. Increasing preclinical and clinical studies/evidence indicate that gut microbes are a possible susceptibility factor for the progression of neurological disorders, including Alzheimer’s disease (AD), Parkinson’s disease (PD), multiple sclerosis (MS) and autism spectrum disorder (ASD). In this review, we discuss the crucial connection between the gut microbiota and the central nervous system, the signaling pathways of multiple biological systems and the contribution of gut microbiota-related neurological disorders.

OBJECTIVES To determine the effect of Epidural steroids on postoperative pain and hospital stay in patients having a single-level lumbar discectomy. METHODOLOGY A quasi-experimental study was conducted at the Neurosurgery department, Qazi Hussain Ahmad Medical Complex, Nowshera. Sixty patients with a mean age of 40.3±9.21 years were enrolled. The enrolled patients were divided into two groups: Group A patients were given intraoperative epidural methylprednisolone 80 mg, while Group B patients were given only normal saline. Pre- and post-operative pain was assessed after 6 hours, 12 hours and at the discharge time from the hospital. The duration of hospital stay was also noted. RESULTS Out of 60 patients, 55.0% were male, and 45.0% were female, with an overall mean age of 40.3±9.21 years. The VAS score in Group A was 0.82±0.26 compared to 2.30±0.71 in group B (P

Abstract Multidrug resistance among pathogenic bacteria is imperilling the worth of antibiotic infection, which has become an emerging problem, which previously transformed the veterinary sciences. Since its discovery, many antibiotics have been effective in treating bacterial infections in animals. Escherichia coli, a bacterium, is one of the reservoirs of antibiotic resistance genes in a community. The current use of antibiotics and demographic factors usually increase multidrug resistance. Genetically, the continuous adoption of environmental changes by E. coli allows it to acquire many multidrug resistance. During the host's life, antimicrobial resistance rarely poses a threat to the E. coli strain and pressure, similar to that of a flexible animal lower intestine. In this review, we describe the E. coli antibiotic drug–resistance mechanism driving transmission, the causes of transmission and the harmful effects on animal health.

Because of its exceptional qualities, aluminium composites are frequently employed in aircraft applications. Aluminium alumina composite was manufactured in-situ using the AA-6061 T6 matrix alloy and 3 %age, 6 %age (Nichrome) powder. Optical metallography, Image Analysis, and micro hardness tests were used to assess the in-situ composites. The specimens then liquid preheated at 550 °C for 3 hours and then aged at 180 °C for 7 hours to improve the durability in mechanical characteristics. In comparison to the as cast samples, the heat-treated samples showed significantly higher mechanical properties. AA-6061 T6 matrix composite reinforced with in-situ alumina particle with TiC was also studied for grain refining. The Al-4.5 % Ti-1.5 % C master alloy has been shown to be an effective grain refiner.

The interaction of post-traumatic stress disorder (PTSD) and atherosclerosis (AS) increase the risk of mortality. Metabolism and immunity play important roles in the comorbidity associated with PTSD and AS. The adenosine monophosphate-activated protein kinase/mammalian target of rapamycin and phosphatidylinositol 3-kinase/Akt pathways are attractive research topics in the fields of metabolism, immunity, and autophagy. They may be effective intervention targets in the prevention and treatment of PTSD comorbidity with AS. Herein, we comprehensively review metabolic factors, including glutamate and lipid alterations, in PTSD comorbidity with AS and discuss the possible implications in the pathophysiology of the diseases.

Schistosomiasis is one of the neglected tropical diseases that affect millions of people worldwide. Globally, it affects economically poor countries, typically due to a lack of proper sanitation systems, and poor hygiene conditions. Currently, no vaccine is available against schistosomiasis, and the preferred treatment is chemotherapy with the use of praziquantel. It is a common anti-schistosomal drug used against all known species of Schistosoma. To date, current treatment primarily the drug praziquantel has not been effective in treating Schistosoma species in their early stages. The drug of choice offers low bioavailability, water solubility, and fast metabolism. Globally drug resistance has been documented due to overuse of praziquantel, Parasite mutations, poor treatment compliance, co-infection with other strains of parasites, and overall parasitic load. The existing diagnostic methods have very little acceptability and are not readily applied for quick diagnosis. This review aims to summarize the use of nanotechnology in the treatment, diagnosis, and prevention. It also explored safe and effective substitute approaches against parasitosis. At this stage, various nanomaterials are being used in drug delivery systems, diagnostic kits, and vaccine production. Nanotechnology is one of the modern and innovative methods to treat and diagnose several human diseases, particularly those caused by parasite infections. Herein we highlight the current advancement and application of nanotechnological approaches regarding the treatment, diagnosis, and prevention of schistosomiasis.

Infections caused by helminths are responsible for severe public health problems and economic burden on continental scale. Well-timed and precise diagnosis of helminth infections is critical for taking by appropriate approaches for pathogen control. Circulating miRNAs are stable diagnostic tool for different diseases found in a variety of body fluid. As diagnostic biomarkers in infectious diseases, miRNAs detection in body fluids of helminth infected hosts is growing promptly. Uncovering miRNAs is a relatively new tool, used for early-stage detection of helminth infection from experimental or non-invasive clinical samples. miRNAs can be detected in body fluids such as serum, saliva, urine, and tissues of helminth infected host, mainly blood offering important benefits for diagnosis accurately. In this review, we discuss different characteristics of helminth parasite-derived circulating and EV miRNAs, supporting its potential uses in for helminth diagnosis and treatment efficiency.

Abstract Aloe vera is reputed to have medicinal properties. For centuries, it has been used for an array of ailments such as mild fever, wounds and burns, gastrointestinal disorders, diabetes, sexual vitality and fertility problems to cancer, immune modulation, AIDS and various skin infections. In present study, antibacterial activity of aloe vera gel extracts was tested against some common skin infection pathogens, that is, Escherichia coli, Shigella, Salmonella spp. and Staphylococcus aureus all were recorded positive. Antibiotic resistance and susceptibility pattern of above isolates were also studied against 10 clinically significant antibiotics (ampicillin [AMC], amoxicillin, augmentin, cefotaxime, ceftazidime [CAZ], cefuroxime [CXM], ciprofloxaci, tetracycline, cefpodoxime and imipenem). AMC and CXM were found to be most effective antibiotic followed by CXM with highest efficacy against Gram‐negative bacteria. In case of CAZ showed highest efficacy was showed against Gram‐positive bacteria. Aloe vera leave gel was extracted with four different solvent‐like aloe vera leaf extract, root extract, leaf ethanol extract and root ethanol extract; however, Gram‐negative as well Gram‐positive isolates was found highest susceptibility with aloe leaf and aloe root ethanol extract. Moderate sensitivity observed with aloe leaf extract and aloe root extract against both Gram‐positive as well as Gram‐negative bacterial isolates. This result showed that ethanol extracts of aloe vera both leaf and root can be used alongside conventional antibiotics to fight agents of infections that are so prevalent in the skin infection.

Purpose: Mycoplasma capricolum subsp. capripneumoniae (Mccp) causes a severe, usually fatal disease in goats known as Contagious Caprine Pleuropneumonia (CCPP). CCPP is listed by OIE as a notifiable animal diseases, causing economic losses in terms of high morbidity and mortality. Thus far, very limited information is available on the molecular characterization of the unique Mccp strains prevalent in Pakistan. The study was aimed to isolate Mccp local strain for the development of diagnostics and vaccines. Methods: Samples were collected during November 2017–December 2018 at Northern areas of Pakistan from 10 goat flocks each in Gilgit-Baltistan, Chitral, Swat, Buner, and Hazara. 900 samples were collected; nasal swabs (n = 400), tracheal swabs (n = 150) from naturally infected goats showing clinical signs of CCPP, and lungs tissue (n = 200), pleural fluid (n = 150) from goats at necropsy. Results: The clinical signs recorded were mucopurulent nasal discharges, cough, abdominal respiration and hyperthermia. The post-mortem revealed, pulmonary consolidation, fibrinous pleuropneumonia, and accumulation pleural fluid. The fried egg like growth was observed on agar in 16 (4%), 11 (7.3%), 38 (19%), and 24 (16%) nasal swab, tracheal swabs, lungs and pleural fluid samples, respectively. PCR targeting 16S rRNA gene revealed isolates, belongs to Mycoplasma mycoides cluster, in 72 (8%) samples. Forty one (4.5%) isolates were Mccp by specie specific PCR generating an amplicon of 316 bp. Conclusions: We successfully isolated local strain of Mccp for the first time in Pakistan. This Mccp strain could be further utilized for the development of diagnostics and control measures against Mccp infection in goats.

The molecular consequences of cancer associated mutations in Acute myeloid leukemia (AML) linked factors are not very well understood. Here, we interrogated the COSMIC database for missense mutations associated with the RUNX1 protein, that is frequently mis-regulated in AML, where we sought to identify recurrently mutated positions at the DNA-interacting interface. Indeed, six of the mutated residues, out of a total 417 residues examined within the DNA binding domain, evidenced reduced DNA association in in silico predictions. Further, given the prominence of RUNX1’s compromised function in AML, we asked the question if the mutations themselves might alter RUNX1’s interaction (off-target) with known FDA-approved drug molecules, including three currently used in treating AML. We identified several AML-associated mutations in RUNX1 that were calculated to enhance RUNX1’s interaction with specific drugs. Specifically, we retrieved data from the COSMIC database for cancer-associated mutations of RUNX1 by using R package “data.table” and “ggplot2” modules. In the presence of DNA and/or drug, we used docking scores and energetics of the complexes as tools to evaluate predicted interaction strengths with RUNX1. For example, we performed predictions of drug binding pockets involving Enasidenib, Giltertinib, and Midostaurin (AML associated), as well as ten different published cancer associated drug compounds. Docking of wild type RUNX1 with these 13 different cancer-associated drugs indicates that wild-type RUNX1 has a lower efficiency of binding while RUNX1 mutants R142K, D171N, R174Q, P176H, and R177Q suggested higher affinity of drug association. Literature evidence support our prediction and suggests the mutation R174Q affects RUNX1 DNA binding and could lead to compromised function. We conclude that specific RUNX1 mutations that lessen DNA binding facilitate the binding of a number of tested drug molecules. Further, we propose that molecular modeling and docking studies for RUNX1 in the presence of DNA and/or drugs enables evaluation of the potential impact of RUNX1 cancer associated mutations in AML.

The present cross-sectional survey was carried out to investigate the distribution and risk factors of FMD in Pakistan’s northern border regions. About 385 serum samples were compiled from small ruminants (239) and large ruminants (146) and tested using 3ABC-Mab-bELISA. An overall apparent seroprevalence of 67.0% was documented. The highest seroprevalence of 81.1% was reported in the Swat, followed by 76.6% in Mohmand, 72.7% in Gilgit, 65.6% in Shangla, 63.4% in Bajaur, 46.6% in Chitral and lowest 46.5% in Khyber region. Statistically significant variations in seroprevalence of 51.5%, 71.8%, 58.3%, and 74.4% were recorded in sheep, goats, cattle, and buffaloes, respectively. From the different risk factors investigated, age, sex, species of animal, seasons, flock/herd size, farming methods, outbreak location, and nomadic animal movement were found to be significantly associated (p < 0.05) with the seroprevalence of FMD. It was concluded that proper epidemiological study, risk-based FMD surveillance in small ruminants, vaccination strategy, control measures for transboundary animal movement, collaborations, and awareness programs need to be practiced in the study regions to investigate the newly circulating virus strains in large and small ruminants and associated factors for the wide seroprevalence to plan proper control policies to bound the consequence of FMD in the region.

Abstract Background The Eucotylidae Cohn, 1904 (Superfamily: Microphalloidea), is a family of digeneans parasitic in kidneys of birds as adults. The group is characterized by the high level of morphological similarities among genera and unclear systematic value of morphological characters traditionally used for their differentiation. In the present study, we sequenced the complete or nearly complete mitogenomes (mt genome) of two eucotylids representing the genera Tamerlania (T. zarudnyi) and Tanaisia (Tanaisia sp.). They represent the first sequenced mt genomes of any member of the superfamily Microphalloidea. Methods A comparative mitogenomic analysis of the two newly sequenced eucotylids was conducted for the investigation of mitochondrial gene arrangement, contents and genetic distance. Phylogenetic position of the family Eucotylidae within the order Plagiorchiida was examined using nucleotide sequences of mitochondrial protein-coding genes (PCGs) plus RNAs using maximum likelihood (ML) and Bayesian inference (BI) methods. BI phylogeny based on concatenated amino acids sequences of PCGs was also conducted to determine possible effects of silent mutations. Results The complete mt genome of T. zarudnyi was 16,188 bp and the nearly complete mt genome of Tanaisia sp. was 13,953 bp in length. A long string of additional amino acids (about 123 aa) at the 5′ end of the cox1 gene in both studied eucotylid mt genomes has resulted in the cox1 gene of eucotylids being longer than in all previously sequenced digeneans. The rrnL gene was also longer than previously reported in any digenean mitogenome sequenced so far. The TΨC and DHU loops of the tRNAs varied greatly between the two eucotylids while the anticodon loop was highly conserved. Phylogenetic analyses based on mtDNA nucleotide and amino acids sequences (as a separate set) positioned eucotylids as a sister group to all remaining members of the order Plagiorchiida. Both ML and BI phylogenies revealed the paraphyletic nature of the superfamily Gorgoderoidea and the suborder Xiphidiata. Conclusions The average sequence identity, combined nucleotide diversity and Kimura-2 parameter distances between the two eucotylid mitogenomes demonstrated that atp6, nad5, nad4L and nad6 genes are better markers than the traditionally used cox1 or nad1 for the species differentiation and population-level studies of eucotylids because of their higher variability. The position of the Dicrocoeliidae and Eucotylidae outside the clade uniting other xiphidiatan trematodes strengthened the argument for the need for re-evaluation of the taxonomic content of the Xiphidiata.

Outsourcing has been getting a significant growth for the last few years. Organizations tend to outsource Information Technology (IT), primarily to take advantage of the availability of qualified, trained and skilled workforce in low cost countries across the globe. Outsourcing of IT and software maintenance seem very promising, but a number of factors, risks, and challenges associated with the outsourcing process that make the sourcing decision very complicated. The present study aimed at gaining in-depth understanding of the three aspects of outsourcing, namely; perceived benefits of IT outsourcing, influencing factors of IT outsourcing and software maintenance offshoring. The findings of the current study will lead us to develop a sourcing framework for outsourcing decision as well as a decision support system for software maintenance. A systematic literature review is performed that presents perceived benefits of IT outsourcing, the influencing factors of IT outsourcing and software maintenance. Furthermore, the identified factors are analyzed based on their occurrences in literature as well as chi square test is performed to derive the significant differences amongst the factors based on decades. Similarly, critical success factors are derived both for IT outsourcing and software maintenance offshoring. Our article shows that how the critical success factors impact the IT as well the software maintenance in global delivery perspective. The findings of the current study will help the IT experts and decision makers in making suitable sourcing decisions.

Global Software Development (GSD) has been an emerging trend in the development of software globally, for the last two decades. Information Technology (IT) outsourcing includes application development, application maintenance, infrastructure management and business process outsourcing. Software maintenance aims to keep the IT system operational and to fulfill the client requirements. The maintenance is considered the longest phase of software life cycle that consumes about 60-70% of the total software budget. Maintenance of software is not only time consuming but also requires a significant human resources’ ratio. Mostly, software acquisition and maintenance consume a big portion of the total IT budget. The current study aims to evaluate the findings of the systematic literature review and to derive a list of critical success factors regarding offshore outsourcing decision of application maintenance. Thus, an empirical study is performed to validate the influencing factors that were identified by using systematic literature review. These factors are further validated by 93 outsourcing experts from 30 different countries. The collected data through online survey is analyzed based on variables such as respondents experience level, respondents’ locations (continents), experts’ positions. Similarly, the data is analysed based on Chi square test (linear by linear association) and Spearman Rank Correlation. Additionally, the identified factors through survey and systematic literature review are ranked by two different methods. Consequently, a project assessment model is proposed, based on the critical success factors for the sourcing decision of application maintenance.

In this paper we proposed a solar cell having model “Back Contact/CZTS/ZnCdS/ZnO/Front Contact”. CZTS is working as an absorber layer, ZnCdS as a buffer layer and ZnO as a window layer with back and front contacts. The Zn content was varied from 0% to 10% and bad gap was changed from 2.42 to 2.90 eV as described in the literature. The impact of this band gap variation has been observed on the performance of solar cell by using SCAPS-1D software. The efficiency was varied due to variation in bandgap of ZnCdS thin film layer. The simulation was carried out at 300K under A.M 1.5 G 1 Sun illumination. The energy bandgap diagram has been taken from SCAPS to explain the different parameters of solar cell. The effect of ZnCdS having different bandgap values was observed. Then the thickness of CZTS layer was varied to check its effect and hence at 3.0 um gave the imporved efficiency of 13.83% roundabout. After optimization of CZTS layer thickness, the effect of working temperature was examined on the performance of solar cell. The absorption coefficient variation from 1E+4 to 1E+9 cm-1 caused major effects on the characteristics parameters of solar cell along with on J-V characteristics and Quantum Efficiency curve. At 1E+9 cm-1 absorption coefficient the efficiency of solar cell boost up to 16.24%. This is the remarkable improvement in the efficiency of solar cell from 13.82% to 16.24%. After optimization of all parameters, simulation was run at 280K, having CZTS thickness of 3.5 um, with 10% content Zn in ZnCdS (2.90 eV), and absoption coefficient of 1E+9, the model efficiency reached up to 17.6% with Voc of 0.994 V, Jsc 26.1 mA/cm2 and Fill factor was 71.4%.

In this research work, the electrical simulation of 1D5P model solar cell is done using LTSpice-IV simulation software. In this work effect of environmental conditions i.e temperature, solar irradiance, and parasitic parameters i.e series as well as shunt resistances was carried out. It has been discovered that as temperature increases the performance of solar cell decrease because temperature causes to increase the recombination phenomenon and hence lower the performance. However, when the temperature rises from 00C to 500C, the I-V and P-V curves move to the origin showing the negative effect of increasing temperature on the solar cell. Solar irradiance has major role on the performance of solar cell. As solar irradiance increases from 250 Wm-2 to 1000 Wm-2, the performance of solar cell increases accordingly and I-V as well as P-V curve moves away from the origin. It is concluded that for different series resistances, I-V along with P-V characteristic of 1D5P model solar cell varies, as at 0.02Ω series resistance, a maximum short circuit current and maximum power is obtained. But when series resistance increased up 2 ohm only, the I-V and P-V curves moves to origin drastically. Shunt Resistance is the path of reverse current of the cell. As the shunt resistance increases, the path for reverse current decreased, hence all current goes to load, hence maximum power is obtained. Similarly when the value of shunt resistance decreased, the voltage-controlled section of I-V characteristics curve is moved closer to the origin hence reduced the solar cell performance. It's critical to understand how different factors affect the I-V and P-V characteristics curves of solar cells. The open circuit voltage, short circuit current and maximum power is all variable. The influence of these factors may be extremely beneficial when tracking highest power point of a solar cell applying various methods.

The purpose of this study is to determine mathematical communication skills that assessed from introvert and extrovert personality types, to determine the strengths and weaknesses of each personality type. This research is a qualitative descriptive study. The class subjects are students of SMA Kartika III-1 Banyubiru, grade XI MIPA 2 (Mathematics and Natural Science). The subjects of the research are 2 introvert and 2 extrovert personality types' students. The procedures of collecting data are written tests, interviews, questionnaires and documentation. The results of this study show that mathematical communication skills in terms of introvert personality type are able to meet 1 indicator. That is the ability to explain ideas, situations and mathematical relations in writing. In contrast, the extrovert personality type meets 2 indicators of mathematical communication skills. There is the ability to express daily events in language or mathematical symbols and the ability to read mathematical symbols. The strengths of introvert personality type are able to explain ideas verbally and in writing. Then the weaknesses are difficult to understand the problem of mathematical images and symbols. In contrast to the introvert, extrovert personality's strengths are able to understand mathematical symbols, and then its weaknesses are hard to understand mathematics questions.

Abstract Cooperative aerial wireless networks composed of small unmanned aerial vehicles(UAVs) are easy and fast to deploy and provide on the fly communication facilities in situations where part of the communication infrastructure is destroyed and the survivors need to be rescued on emergency basis. In this article, we worked on such a cooperative aerial UAV-based wireless network to connect the two participating stations. The proposed method provides on the fly communication facilities to connect the two ground stations through a wireless access point (AP) mounted on a UAV using the IEEE 802.11a/b/g/n. We conducted our experiments both indoor and outdoor to investigate the performance of IEEE 802.11 protocol stack including a/b/g/n. We envisioned two different cases: line of sight (LoS) and non-line of sight (NLoS). In LoS, we consider three different scenarios with respect to UAV altitude and performed the experiments at different altitudes to measure the performance and applicability of the proposed system in catastrophic situations and healthcare applications. Similarly, for NLoS, we performed a single set of experiments in an indoor environment. Based on our observations from the experiments, 802.11n at 2.4 GHz outperforms the other IEEE protocols in terms of data rate followed by 802.11n at 5 GHz band. We also concluded that 802.11n is the more suitable protocol that can be practiced in disastrous situations such as rescue operations and healthcare applications.

Application maintenance consumes a considerable amount of an organization's time and resources each year. Almost 60% of IT budget is spent alone on application maintenance. The reason of offshore outsourcing of application maintenance is not only the reduction of maintenance cost but to free up the resources and to keep the focus on core products. Offshore outsourcing is a common business strategy that is used by companies to achieve cost savings about 20-50%. However, the decision making process of application maintenance is a complex phenomenon. It is based on a set of influencing factors, clients' requirements and nature of the project. Hence, the current study is aimed at the in-depth investigation of the complex sourcing decision process of application maintenance. Accordingly, a systematic literature review is performed to determine the influencing factors and critical success factors that will be used by the decision makers for the evaluation of projects before making the outsourcing decisions. A total of 15 influencing factors out of 52 selected papers were identified. Based on the defined criteria, amongst the identified factors, only 10 factors were ranked as critical success factors, which are employees' skills, cost, legal requirements, infrastructure, communication, knowledge transfer, maturity level, project management, language barrier and frequent requirements changes. Consequently, a sourcing model was proposed based on the identified critical success factors that help the IT managers and domain experts in making appropriate outsourcing decisions.

The uropathogens is the main cause of urinary tract infection (UTI). The aim of the study was to isolate bacteria from urine samples of UTI patients and find out the susceptibility of isolated bacteria. Bacteria were identified using both conventional and molecular methods. Sanger sequence procedure used for 16S ribosomal RNA and phylogenetic analysis was performed using Molecular Evolutionary Genetics Analysis (MEGA-7) software. In this study, Escherichia coli, Klebsiella pneumonia, Staphylococcus were reported as 58, 28 and 14.0% respectively. Phylogenetic tree revealed that 99% of sample No. Ai (05) is closely related to E. coli to (NR 114042.1 E. coli strain NBRC 102203). Aii (23) is 99% similar to K. pneumoniae to (NR 117686.1 K. pneumonia strain DSM 30104) and 90% Bi (48) is closely linked to S. aureus to (NR 113956.1 S. aureus strain NBRC 100910). The antibiotic susceptibility of E. coli recorded highest resistance towards ampicillin (90%) and least resistant to ofloxacin (14%). Some of the other antibiotics such amoxicillin, ciprofloxacin, gentamicin, ceftazidime, cefuroxime and nitrofurantoin resistance were observed 86, 62, 24, 55, 48 and 35% respectively. The cefuroxime showed the highest antibiotic resistance against K. pneumoniae with 85% followed by amoxicillin, ciprofloxacin, gentamicin, ceftazidime, ampicillin and nitrofurantoin resulted in 60, 45, 67, 70, 75 and 30% respectively. The resistance of S. aureus against erythromycin, cefuroxime and ampicillin were found with 72%. The resistance against amoxicillin, gentamicin, ceftazidime and ceftriaxone found 57, 43, 43 and 15% respectively. Phylogenetic analysis shows that sequences are closely related with the reference sequences and E. coli is the dominant bacteria among UTI patients and is resistant to the commercially available antibiotics.

OBJECTIVES: To determine the post-operative neurological outcome of intradural spinal tumors in terms of improvement in McCormick classification scheme. METHODOLOGY: Total of 95 patients diagnosed as case of intradural spinal tumor as per operational definition with any McCormick grade were analyzed. After admission and complete neurological examination, pre-operative McCormik’s grades of each patient were determined and followed for one month after surgery. During their stay they were assessed for neurological improvement as per McCormick grade. RESULTS: There were total 95 patients presenting in OPD with the mean age of 45±12.36 years. There were 42% male and 58% female. There were 62% patients who were improved in neurological outcome while 38% patients were not improved. CONCLUSION: Surgical removal of spinal tumor is beneficial to the affected individual and has positive effect on his life.

The rapid spread of the internet over the last two decades has prompted more and more companies to deploy their work internationally. The offshoring strategy enables organizations to cut down costs, boost shareholder value, acquire a competitive advantage, reduce cycle time, increase workforce flexibility, generate revenue and focus on their core business. The number of worldwide software development projects has increased due to globalization. Global Software Development (GSD) projects are forecast to grow by 20% to 30% in countries like India and China. The outsourcing experts choose one of the suitable models from the available global delivery options to deliver services in the global software paradigm. However, adopting the appropriate model for application maintenance is a complicated process. In addition, the right model is selected based on various influencing factors, type of the project and client requirements. Additionally, sufficient domain expertise is necessary for the decision making of offshore outsourcing. Currently, there is no dynamic and automated tool for the decision making of application maintenance offshoring. Therefore, this study presents an Offshoring Decision Support System (OffshoringDSS), an automated and novel tool to make the offshoring decisions of application maintenance. The suggested tool is based on the Analytic Hierarchy Process (AHP) technique. The tool automatically performs all the calculations involved in the decision making and ranks the sourcing models.

The antioxidant potential and total phenolic contents of a red alga Agardhiella robusta (Grevi.) Borg belonging to Rhodophycota collected from coastal areas of Karachi, Pakistan was investigated through various in vitro protocols. Antioxidant potential measured through total antioxidant determination, total phenolic contents, ferric reducing antioxidant power (FRAP) and DPPH analysis. The antioxidant components were initially extracted in methanol and were further fractionated in solvents of different polarity. The results showed that the petroleum ether fraction exhibited highest DPPH radical scavenging activity, i.e. (79.5±1.9%) at a concentration of 1000ug/ml with IC50 286.13±0.34 μg/mL relative to the BHT. While Methanol extract showed highest phenolic contents i.e. 1542.5 ±1.2 GAEmg/mL, highest FRAP values i.e. 18.95± TEuM/mL as well as least IC50 value of 25.81 ± 0.65 μg/mL which indicated its potential for scavenging the free radicals. On the basis of these results obtained here Agardhiella robusta (Grevi.) Borg considered as a rich source of antioxidant. [ABSTRACT FROM AUTHOR]

Advances in technology are not only changing the world around us but also driving the wireless industry to develop the next generation of network technology. There is a lot of buzz building over the advent of 5G that will facilitate the entire planet through continuous and ubiquitous communication connecting anybody to anything, anywhere, anytime, and anyhow regardless of the device, service, network, or geographical existence. 5G will also prove to be a paradigm shift including high carrier frequencies with massive bandwidths, having a large number of antennas, and with an extreme base station and device densities. In this paper, we investigate the potential beneficiaries of 5G and identify the use-cases, where 5G can make an impact. In particular, we consider three main use-cases: vehicle-to-everything (V2X) communication, drones, and healthcare. We explore and highlight the problems and deficiencies of current cellular technologies with respect to these use-cases and identify how 5G will overcome those deficiencies. We also identified the open research problems and provide possible future directions to cope with those issues.

The present study aimed for the extraction of color dyes from different sources, such as Brassica oleracea, Brassica campestris, Citrus limon, Citrus limetta, Tagetes erecta, Spinacea oleracea, Beta vulgaris, Rosa indica and Curcuma longa. The leftovers of such plants were mainly used for color dye extraction and their confirmation using spectrophotometric analysis. The specific color pigments like carotenoids, anthocyanin, chlorophyll and betanin were found to be the main coloring agents that impart specific color to the samples. Among all these samples, the maximum yield was obtained from C. limetta aqueous peel extract, and among all the temperatures employed room temperature was found out to be the most suitable temperature for the stability of color extracts. The extracted colors were utilized in candy making and sugar syrup making and were also used for coloring various foods stuffs. Moreover, the extracted color dyes were applied for dyeing purposes on cotton cloth with alum showing better and more enhancing color fastness results than the lime.