1. RNA thermometers are widespread upstream of ABC transporter genes in bacteria.
- Author
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Tong AY, Tong EL, Hannani MA, Shaffer SN, Santiago D, Ferré-D'Amaré AR, Passalacqua LFM, and Abdelsayed MM
- Subjects
- RNA, Bacterial metabolism, RNA, Bacterial genetics, RNA, Bacterial chemistry, Gene Expression Regulation, Bacterial, Bacterial Proteins metabolism, Bacterial Proteins genetics, Bacterial Proteins chemistry, Crystallography, X-Ray, Escherichia coli metabolism, Escherichia coli genetics, ATP-Binding Cassette Transporters metabolism, ATP-Binding Cassette Transporters genetics, ATP-Binding Cassette Transporters chemistry
- Abstract
RNA thermometers are temperature-sensing non-coding RNAs that regulate the expression of downstream genes. A well-characterized RNA thermometer motif discovered in bacteria is the ROSE-like element (repression of heat shock gene expression). ATP-binding cassette (ABC) transporters are a superfamily of transmembrane proteins that harness ATP hydrolysis to facilitate the export and import of substrates across cellular membranes. Through structure-guided bioinformatics, we discovered that ROSE-like RNA thermometers are widespread upstream of ABC transporter genes in bacteria. X-ray crystallography, biochemistry, and cellular assays indicate that these RNA thermometers are functional regulatory elements. This study expands the known biological role of RNA thermometers to these key membrane transporters., Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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