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6 results on '"Hansen, Martin C."'

1. Changes in rRNA levels during stress invalidates results from mRNA blotting: fluorescence in situ rRNA hybridization permits renormalization for estimation of cellular mRNA levels

Author

Hansen, Martin C., Nielsen, Allan K., Molin, Soren, Hammer, Karin, and Kilstrup, Mogens

Subjects
Gene expression -- Analysis, Molecular biology -- Methods, In situ hybridization -- Usage, RNA -- Measurement, Biological sciences
Abstract

This article evaluates the northern blotting technique employed in gene expression investigations and a need for a normalization procedure for presenting experimental data. Research describes the use of fluorescence in situ hybridization technique in such studies.

Published
2001

2. Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration

Author

Hansen, Martin C., Palmer, Robert J., Jr., Udsen, Camilla, White, David C., and Molin, Soren

Subjects
Microbiological research -- Reports, Cytochemistry -- Research, Fluorescence -- Physiological aspects, Streptococcus -- Physiological aspects, Oxygen -- Physiological aspects, Hydrogen-ion concentration -- Physiological aspects, Anaerobic bacteria -- Physiological aspects, Gram-positive bacteria -- Research, Microbial mats -- Research, Bacterial growth -- Research, Biological sciences
Abstract

Green fluorescent protein (GFP) fluorescence in Streptococcus gordonii was assessed under low-oxygen concentration and low-pH conditions. GFP, it was found, can be used in studies of oxygen-tolerant anaerobic bacteria. Densely packed, flow cell-grown biofilms of S. gordonii do not develop oxygen gradients that inhibit GFP fluorescence development. The transient nature of GFP fluorescence in acid-producing bacteria can often be overcome in flowcells.

Published
2001

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