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10 results on '"Hansong Jin"'

1. Evaluating personalized circulating tumor DNA detection for early‐stage lung cancer

Author

Haihua Huang, Zhentian Kai, Yuchen Wang, Xiaomiao Zhang, Jin Wang, Wei Zhang, Qian Xue, Hang Zhang, Hansong Jin, Peize Meng, Shuilong Zhang, Yueyue Yang, Honghua Yang, Wanning Liang, Guangbing Zha, Peng Luo, Yan Xu, Weiwei Shi, and Zheng Ruan

Subjects
biomarkers, biotechnology, genomics, lung cancer, molecular diagnosis, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Circulating tumor DNA (ctDNA) has been widely used as a minimally invasive biomarker in clinical routine. However, a number of factors such as panel design, sample quality, patients' disease stages are known to influence ctDNA detection sensitivity. In this study, we systematically evaluated common factors associated with the variability of ctDNA detection in plasma and investigated ctDNA abundance in bronchoalveolar lavage (BAL). Whole exome profiling was conducted on 61 tumor tissue samples to identify tumor‐specific variants, which were then used to design personalized assay MarRyDa® for ctDNA detection. DNA extracted from BAL fluid and plasma were genotyped using MarRyDa® platform. Our analysis showed that histological subtypes and disease stages had significant differences in ctDNA detection rate. Furthermore, we found that DNA purified from BAL supernatants contains the highest levels of ctDNA compared with BAL precipitates and plasma; therefore, utilizing BAL supernatants for tumor detection might provide additional benefits. Finally, we demonstrated that tumor cellularity played significant roles in the design of personalized ctDNA panel which eventually impacts ctDNA detection sensitivity. We suggest setting a flexible criteria for sample quality control and utilization of BAL might benefit more patients in clinics.

Published
2024
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2. Manganese-Based Prussian Blue Nanocatalysts Suppress Non-Small Cell Lung Cancer Growth and Metastasis via Photothermal and Chemodynamic Therapy

Author

Danruo Fang, Zeyu Liu, Hansong Jin, Xiulin Huang, Yongxin Shi, and Suqin Ben

Subjects
MnPB nanoparticles, non-small cell lung cancer, chemodynamic therapy, photothermal therapy, metastatic lung cancer, Biotechnology, TP248.13-248.65
Abstract

Based on the safety of prussian blue (PB) in biomedical application, we prepared manganese-based prussian blue (MnPB) nanocatalysts to achieve enhanced photothermal therapy and chemodynamic therapy. And we conducted a series of experiments to explore the therapeutic effects of MnPB nanoparticles (NPs) on non-small cell lung cancer (NSCLC) in vivo and in vitro. For in vitro experiments, the MnPB NPs suppressed growth of A549 cells by reactive oxygen species upregulation and near-infrared irradiation. Moreover, the MnPB NPs could inhibit lung cancer metastasis through downregulating the matrix metalloproteinase (MMP)-2 and MMP-9 expression in A549 cells. And for in vivo experiments, the MnPB NPs inhibited the growth of xenografted tumor effectively and were biologically safe. Meanwhile, Mn2+ as a T1-weighted agent could realize magnetic resonance imaging-guided diagnosis and treatment. To sum up, the results in this study clearly demonstrated that the MnPB NPs had remarkable effects for inhibiting the growth and metastasis of NSCLC and might serve as a promising multifunctional nanoplatform for NSCLC treatment.

Published
2022
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3. PPy@Fe3O4 Nanoparticles Inhibit Tumor Growth and Metastasis Through Chemodynamic and Photothermal Therapy in Non-small Cell Lung Cancer

Author

Danruo Fang, Hansong Jin, Xiulin Huang, Yongxin Shi, Zeyu Liu, and Suqin Ben

Subjects
PPy@Fe3O4 nanoparticles, photothermal therapy, chemodynamic therapy, non-small cell lung cancer, tumor metastasis, Chemistry, QD1-999
Abstract

Non-small cell lung cancer (NSCLC) is considered to be a principal cause of cancer death across the world, and nanomedicine has provided promising alternatives for the treatment of NSCLC in recent years. Photothermal therapy (PTT) and chemodynamic therapy (CDT) have represented novel therapeutic modalities for cancer treatment with excellent performance. The purpose of this research was to evaluate the effects of PPy@Fe3O4 nanoparticles (NPs) on inhibiting growth and metastasis of NSCLC by combination of PTT and CDT. In this study, we synthesized PPy@Fe3O4 NPs through a very facile electrostatic absorption method. And we detected reactive oxygen species production, cell apoptosis, migration and protein expression in different groups of A549 cells and established xenograft models to evaluate the effects of PPy@Fe3O4 NPs for inhibiting the growth of NSCLC. The results showed that the PPy@Fe3O4 NPs had negligible cytotoxicity and could efficiently inhibit the cell growth and metastasis of NSCLC in vitro. In addition, the PPy@Fe3O4 NPs decreased tumor volume and growth in vivo and endowed their excellent MRI capability of observing the location and size of tumor. To sum up, our study displayed that the PPy@Fe3O4 NPs had significant synergistic effects of PTT and CDT, and had good biocompatibility and safety in vivo and in vitro. The PPy@Fe3O4 NPs may be an effective drug platform for the treatment of NSCLC.

Published
2021
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4. Tumor microenvironment responsive theranostic agent for enhanced chemo/chemodynamic/photothermal therapy

Author

Jinxia Wang, Wenyan Kong, Hansong Jin, Chunlin Li, Qian Luo, Yu Luo, Chunping Yuan, Jie Lu, Lei Zhang, and Xijian Liu

Subjects
Indocyanine Green, Photothermal Therapy, Contrast Media, Oxides, Surfaces and Interfaces, General Medicine, Hydrogen Peroxide, Silicon Dioxide, Glutathione, Theranostic Nanomedicine, Colloid and Surface Chemistry, Manganese Compounds, Doxorubicin, Cell Line, Tumor, Neoplasms, Tumor Microenvironment, Humans, Physical and Theoretical Chemistry, Reactive Oxygen Species, Biotechnology
Abstract

The specific characteristics of the tumor microenvironment (TME) and monotherapy always lead to poor therapy effects for tumors. Hereby, we have developed a smart multifunctional theranostic agent-SSMID (Se@SiO

Published
2022

5. PPy@Fe3O4 Nanoparticles Inhibit Tumor Growth and Metastasis Through Chemodynamic and Photothermal Therapy in Non-small Cell Lung Cancer

Author

Zeyu Liu, Xiulin Huang, Suqin Ben, Danruo Fang, Yongxin Shi, and Hansong Jin

Subjects
A549 cell, photothermal therapy, tumor metastasis, PPy@Fe3O4 nanoparticles, Cell growth, Chemistry, General Chemistry, Photothermal therapy, medicine.disease, respiratory tract diseases, Metastasis, chemodynamic therapy, In vivo, Apoptosis, medicine, Cancer research, Cytotoxicity, Lung cancer, QD1-999, non-small cell lung cancer, Original Research
Abstract

Non-small cell lung cancer (NSCLC) is considered to be a principal cause of cancer death across the world, and nanomedicine has provided promising alternatives for the treatment of NSCLC in recent years. Photothermal therapy (PTT) and chemodynamic therapy (CDT) have represented novel therapeutic modalities for cancer treatment with excellent performance. The purpose of this research was to evaluate the effects of PPy@Fe3O4 nanoparticles (NPs) on inhibiting growth and metastasis of NSCLC by combination of PTT and CDT. In this study, we synthesized PPy@Fe3O4 NPs through a very facile electrostatic absorption method. And we detected reactive oxygen species production, cell apoptosis, migration and protein expression in different groups of A549 cells and established xenograft models to evaluate the effects of PPy@Fe3O4 NPs for inhibiting the growth of NSCLC. The results showed that the PPy@Fe3O4 NPs had negligible cytotoxicity and could efficiently inhibit the cell growth and metastasis of NSCLC in vitro. In addition, the PPy@Fe3O4 NPs decreased tumor volume and growth in vivo and endowed their excellent MRI capability of observing the location and size of tumor. To sum up, our study displayed that the PPy@Fe3O4 NPs had significant synergistic effects of PTT and CDT, and had good biocompatibility and safety in vivo and in vitro. The PPy@Fe3O4 NPs may be an effective drug platform for the treatment of NSCLC.

Published
2021
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6. Exosomal HOTAIR promotes proliferation, migration and invasion of lung cancer by sponging miR-203

Author

Guoying Deng, Chengcheng Zhang, Jixin Shu, Ke Bi, Zhongqi Wang, Yin Wang, Lisha Xu, Hansong Jin, Jia Yang, Ding Yajie, and Haibin Deng

Subjects
Lung Neoplasms, Biology, General Biochemistry, Genetics and Molecular Biology, Cell Movement, Cell Line, Tumor, medicine, Humans, Lung cancer, Cell Proliferation, General Environmental Science, Regulation of gene expression, Blood Specimen Collection, Exosome Multienzyme Ribonuclease Complex, Cell growth, RNA, HOTAIR, Cell movement, medicine.disease, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell culture, Disease Progression, Cancer research, RNA, Long Noncoding, General Agricultural and Biological Sciences, miR-203
Published
2020
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7. Porous Se@SiO2 nanocomposite promotes migration and osteogenic differentiation of rat bone marrow mesenchymal stem cell to accelerate bone fracture healing in a rat model

Author

Yangyang Hu, Yingjie Kang, Qi Wang, Guoying Deng, Yongxing Zhang, Hansong Jin, Chunlin Li, Qiugen Wang, Taixi Li, and Xiao‐hua Gu

Subjects
Femur fracture, Nanocomposite, Biocompatibility, Chemistry, Organic Chemistry, Mesenchymal stem cell, technology, industry, and agriculture, Biophysics, Pharmaceutical Science, Bioengineering, 02 engineering and technology, General Medicine, Bone healing, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Biomaterials, In vivo, Drug Discovery, Alkaline phosphatase, 0210 nano-technology, Bone regeneration
Abstract

Background: Delay or failure of bone union is a significant clinical challenge all over the world, and it has been reported that bone marrow mesenchymal stem cells (BMSCs) offer a promising approach to accelerate bone fracture healing. Se can modulate the proliferation and differentiation of BMSCs. Se-treatment enhances the osteoblastic differentiation of BMSCs and inhibiting the differentiation and formation of mature osteoclasts. The purpose of this study was to assess the effects of porous Se@SiO2 nanocomposite on bone regeneration and the underlying biological mechanisms. Methods: We oxidized Se2- to develop Se quantum dots, then we used the Se quantum dots to form a solid Se@SiO2 nanocomposite which was then coated with polyvinylpyrrolidone (PVP) and etched in hot water to synthesize porous Se@SiO2 nanocomposite. We used XRD pattern to assess the phase structure of the solid Se@SiO2 nanocomposite. The morphology of porous Se@SiO2 nanocomposite were evaluated by scanning electron microscope (SEM) and the biocompatibility of porous Se@SiO2 nanocomposite were investigated by cell counting kit-8 (CCK-8) assays. Then, a release assay was also performed. We used a Transwell assay to determine cell mobility in response to the porous Se@SiO2 nanocomposite. For in vitro experiments, BMSCs were divided into four groups to detect reactive oxygen species (ROS) generation, cell apoptosis, alkaline phosphatase activity, calcium deposition, gene activation and protein expression. For in vivo experiments, femur fracture model of rats was constructed to assess the osteogenic effects of porous Se@SiO2 nanocomposite. Results: In vitro, intervention with porous Se@SiO2 nanocomposite can promote migration and osteogenic differentiation of BMSCs, and protect BMSCs against H2O2-induced inhibition of osteogenic differentiation. In vivo, we demonstrated that the porous Se@SiO2 nanocomposite accelerated bone fracture healing using a rat femur fracture model. Conclusion: Porous Se@SiO2 nanocomposite promotes migration and osteogenesis differentiation of rat BMSCs and accelerates bone fracture healing, and porous Se@SiO2 nanocomposite may provide clinic benefit for bone tissue engineering.

Published
2019
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9. Porous Se@SiO

Author

Chunlin, Li, Qi, Wang, Xiaohua, Gu, Yingjie, Kang, Yongxing, Zhang, Yangyang, Hu, Taixi, Li, Hansong, Jin, Guoying, Deng, and Qiugen, Wang

Subjects
antioxidant, osteogenic differentiation, Apoptosis, Bone Marrow Cells, migration, Nanocomposites, Rats, Sprague-Dawley, Selenium, Osteogenesis, Animals, Cells, Cultured, Original Research, Fracture Healing, technology, industry, and agriculture, porous Se@SiO2 nanocomposite, bone marrow mesenchymal stem cells, Cell Differentiation, Mesenchymal Stem Cells, Hydrogen Peroxide, X-Ray Microtomography, Silicon Dioxide, Disease Models, Animal, Cytoprotection, Femoral Fractures, Porosity, Signal Transduction
Abstract

Background: Delay or failure of bone union is a significant clinical challenge all over the world, and it has been reported that bone marrow mesenchymal stem cells (BMSCs) offer a promising approach to accelerate bone fracture healing. Se can modulate the proliferation and differentiation of BMSCs. Se-treatment enhances the osteoblastic differentiation of BMSCs and inhibiting the differentiation and formation of mature osteoclasts. The purpose of this study was to assess the effects of porous Se@SiO2 nanocomposite on bone regeneration and the underlying biological mechanisms. Methods: We oxidized Se2- to develop Se quantum dots, then we used the Se quantum dots to form a solid Se@SiO2 nanocomposite which was then coated with polyvinylpyrrolidone (PVP) and etched in hot water to synthesize porous Se@SiO2 nanocomposite. We used XRD pattern to assess the phase structure of the solid Se@SiO2 nanocomposite. The morphology of porous Se@SiO2 nanocomposite were evaluated by scanning electron microscope (SEM) and the biocompatibility of porous Se@SiO2 nanocomposite were investigated by cell counting kit-8 (CCK-8) assays. Then, a release assay was also performed. We used a Transwell assay to determine cell mobility in response to the porous Se@SiO2 nanocomposite. For in vitro experiments, BMSCs were divided into four groups to detect reactive oxygen species (ROS) generation, cell apoptosis, alkaline phosphatase activity, calcium deposition, gene activation and protein expression. For in vivo experiments, femur fracture model of rats was constructed to assess the osteogenic effects of porous Se@SiO2 nanocomposite. Results: In vitro, intervention with porous Se@SiO2 nanocomposite can promote migration and osteogenic differentiation of BMSCs, and protect BMSCs against H2O2-induced inhibition of osteogenic differentiation. In vivo, we demonstrated that the porous Se@SiO2 nanocomposite accelerated bone fracture healing using a rat femur fracture model. Conclusion: Porous Se@SiO2 nanocomposite promotes migration and osteogenesis differentiation of rat BMSCs and accelerates bone fracture healing, and porous Se@SiO2 nanocomposite may provide clinic benefit for bone tissue engineering.

Published
2019

10. Interaction between dietary digestible tryptophan and soy oligosaccharides in broiler chickens: effects on caecal skatole level and microflora

Author

Jing Chen, Hansong Jing, Haiying Liu, Xin Zhu, and Guiqin Yang

Subjects
aromatic amino acid, growth performance, non-digestible carbohydrates, odour-causing compounds, volatile fatty acids, Zoology, QL1-991
Abstract

Objective This study was conducted to evaluate the interactive effects of dietary digestible tryptophan (dTry) and soy oligosaccharides (SO) on growth performance, caecal skatole level, and microflora of broiler chickens aged from 14 to 42 days. Methods Three hundred and sixty broiler chicks were allocated equally to 36 cages at 14-day-of-age according to body weight and gender. Using a 3×2 factorial arrangement, 3 dietary dTry levels (0.18%, 0.23%, and 0.28%) supplemented with 0 or 3.5 g/kg of SO were used to create 6 diets (treatments). Each diet was fed to six replicates of 10 birds (60 birds/treatment), growth performance was measured. Caecal content samples were collected at 42 days of age. Results Results showed that significantly different dTry level×SO interactions were found for average daily gain (ADG), caecal levels of indole, propionic acid, and butyric acid, and microbial Shannon index (p

Published
2023
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