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36 results on '"Hao, Xuefang"'

25. Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Author

Feng,Yakai, Guo,Mengyang, Liu,Wen, Hao,Xuefang, Lu,Wei, Ren,Xiangkui, Shi,Changcan, Zhang,Wencheng, Feng,Yakai, Guo,Mengyang, Liu,Wen, Hao,Xuefang, Lu,Wei, Ren,Xiangkui, Shi,Changcan, and Zhang,Wencheng

Abstract

Yakai Feng,1–5 Mengyang Guo,1 Wen Liu,1 Xuefang Hao,1 Wei Lu,1 Xiangkui Ren,1,2 Changcan Shi,4,5 Wencheng Zhang6 1Department of Polymer Science and Engineering, School of Chemical Engineering and Technology, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin University, 2Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, 3Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin, 4Institute of Biomaterials and Engineering, Wenzhou Medical University, 5Wenzhou Institute of Biomaterials and Engineering, CNITECH, CAS, Wenzhou, 6Department of Physiology and Pathophysiology, Logistics University of Chinese People’s Armed Police Force, Tianjin, People’s Republic of China Abstract: The gene transfection efficiency of polyethylenimine (PEI) varies with its molecular weight. Usually, high molecular weight of PEI means high gene transfection, as well as high cytotoxicity in gene delivery in vivo. In order to enhance the transfection efficiency and reduce the cytotoxicity of PEI-based gene carriers, a novel cationic gene carrier was developed by co-self-assembly of cationic copolymers. First, a star-shaped copolymer poly(3(S)-methyl-morpholine-2,5-dione-co-lactide) (P(MMD-co-LA)) was synthesized using D-sorbitol as an initiator, and the cationic copolymer (P(MMD-co-LA)-g-PEI) was obtained after grafting low-molecular weight PEI. Then, by co-self-assembly of this cationic copolymer and a diblock copolymer methoxy-poly(ethylene glycol) (mPEG)-b-P(MMD-co-LA), microparticles (MPs) were formed. The core of MPs consisted of a biodegradable block of P(MMD-co-LA), and the shell was formed by mPEG and PEI blocks. Finally, after condensation of pEGFP-ZNF580 by these MPs, the plasmids were protected from enzymatic hydrolysis effectively. The result indicated that pEGFP-ZNF580-loaded MP complex

Published
2016

35. Star-shaped copolymer grafted PEI and REDV as a gene carrier to improve migration of endothelial cells

Author

LvThese authors contributed equally to this work., Juan, Hao, Xuefang, Li, Qian, Akpanyung, Mary, Nejjari, Abdelilah, Neve, Agnaldo Luis, Ren, Xiangkui, Feng, Yakai, Shi, Changcan, and Zhang, Wencheng

Abstract

In this work, a biodegradable star-shaped copolymer poly(lactide-co-3(S)-methyl-morpholine-2,5-dione)6(Star-(PLMD)6) was synthesized viaring-opening polymerization (ROP), and subsequently a gene carrier Star-PLMD-g-PEI-g-PEG-CREDVW was prepared by grafting polyethyleneimine (PEI), polyethylene glycol (PEG) and targeting peptide REDV onto Star-(PLMD)6. This gene carrier could form stable micelles to condense pEGFP-ZNF580 through electrostatic interaction. The resulting complexes were biocompatible and showed high efficiency in gene delivery. In addition, these complexes exhibited high selectivity for endothelial cells (ECs), high transfection efficiency and enhanced migration of ECs. The protein level of ZNF580 expression was significantly high (up to 85%), while the control group was only 51%. This combination of degradability, targeting ligand and star-structure strategy exhibits a significant advantage in transfection efficiency and migration of ECs.

Published
2017
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36. CAG W Modified Polymeric Micelles with Different Hydrophobic Cores for Efficient Gene Delivery and Capillary-like Tube Formation.

Author

Hao X, Li Q, Wang H, Muhammad K, Guo J, Ren X, Shi C, Xia S, Zhang W, and Feng Y

Abstract

Recently, polymeric micelles with different biodegradable hydrophobic cores, such as poly(lactide- co -glycolide) (PLGA) and poly(lactide- co -3(S)-methyl-morpholine-2,5-dione) (PLMD), have been used for gene delivery. The biodegradable hydrophobic cores should play an important role in gene delivery. However, little research has focused on selectively promoting proliferation and migration of endothelial cells (ECs) as well as vascularization by altering hydrophobic cores of polymeric micelles. Herein, we prepared two kinds of CAG W peptide (selective adhesion for ECs) modified micelles with PLGA and PLMD as hydrophobic cores, respectively, and poly(ethylene glycol) (PEG) and polyethylenimine (PEI) as mixed hydrophilic shell. Their ability of condensing pEGFP-ZNF580 (pZNF580) to form gene complexes was proved by agarose gel electrophoresis assay. MTT results showed that the relative cell viability of the micelles with PLMD cores was higher than control groups and the micelles with PLGA cores. The cellular uptake ability of these CAG W modified gene complexes was higher than the complexes without CAG W target function. A similar trend was also found in transfection tests in vitro, which further demonstrated the effect of CAG W peptide and different hydrophobic cores on gene delivery. The number of migrated cells treated by the gene complexes with PLGA cores was 82 (nontarget group) and 115 (target group), whereas the complexes with PLMD cores was 88 (nontarget group) and 120 (target group). Capillary-like tube formation of CAG W peptide modified complexes with PLMD core group was much higher (about 6 times) than the PEI(10 kDa)/pZNF580 group. These results demonstrated that transfection efficiency, cell proliferation, migration, and vascularization could be promoted by altering hydrophobic cores and CAG W modification.

Published
2018
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