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A novel cellulase-producing actinomycete strain Gxj-6T, isolated from soil in the cold region (Heihe city, Heilongjiang province, the northernmost part of China), subjected to a taxonomic study using a polyphasic approach. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain Gxj-6T fell within the clade comprising the type strains of species of the genus Microbispora. 16S rRNA gene sequence similarity studies exhibited that species Gxj-6T was most closely related to Microbispora bryophytorum NEAU-TX2-2T (99.45%), Microbispora fusca NEAU-HEGS1-5T (99.41%), Microbispora camponoti 2C-HV3T (99.31%) and Microbispora rosea subsp. rosea JCM 3006T (98.68%). Organism Gxj-6T contained MK-9(H2) as the predominant ubiquinone and C18:0 10-methyl as the major fatty acid. The major polar lipids of culture Gxj-6T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside, three unidentified phospholipids, two unidentified glycolipids and two unidentified lipids. The DNA G+C content of strain Gxj-6T was 71.25 mol%. The morphological and chemotaxonomic properties of the strain are also consistent with those members of the genus Microbispora. Combinated with the lower DNA–DNA relatedness values, phenotypic properties, physiology and biochemistry distinctiveness with other recognized species strains, revealed that strain Gxj-6T is separated from other phylogenetically closely species of the genus Microbispora. Therefore, strain Gxj-6T is considered to represent a novel species of the genus Microbispora, for which the name Microbispora cellulosiformans sp. nov. is proposed. The type strain is Gxj-6T (= CGMCC 4.7605T = DSM 109712T).

Purpose The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization. Methods According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Result The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain Streptomyces sp. G1T has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain Streptomyces sp. G2T has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain Streptomyces sp. G3T to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 °C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer. Conclusion Streptomyces sp. G1T, Streptomyces sp. G2T, and Streptomyces sp. G3T screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources.

The objective of this study was to identify the response of grain yield to plant density and nitrogen rate in spring maize hybrids released from 1970 to 2010 and grown extensively in Northeast China. Twenty-one hybrids were grown for 2 years in Northeast China at densities of 30,000, 52,500, 75,000, and 97,500 plants ha − 1 and N application levels of 0, 150, 300, and 450 kg N ha − 1 . Irrespective of density or nitrogen application rate, grain yields both per plant and per unit area were significantly higher for newer than older hybrids. As plant density increased from 30,000 to 97,500 plant ha − 1 , yield per plant of 1970s, 1980s, 1990s, and 2000s hybrids decreased by 50%, 45%, 46%, and 52%, respectively. The response of grain yield per unit area to plant density was curvilinear. The estimated optimum plant densities were about 58,000, 49,000, 65,000, and 65,000 plants ha − 1 for hybrids released in the 1970s, 1980s, 1990s, and 2000s, respectively. The theoretical optimum densities for the hybrids released from the 1970s to the 2000s increased by 1750 plants ha − 1 decade − 1 . Nitrogen fertilization significantly increased grain yields per plant and per unit area for all hybrids. The theoretical optimum N application rates for high yield for hybrids released in the 1970s and 1980s were about 280 and 360 kg ha − 1 , and the hybrids from the 1990s and 2000s showed highest yield at 330 kg ha − 1 N. No significant difference in the grain yields of 2000s hybrids between the N levels of 150 to 450 kg ha − 1 was found. Significant yield gains per plant and per unit area were found, with average increases of 17.9 g plant − 1 decade − 1 and 936 kg ha − 1 decade − 1 over the period 1970–2010, respectively. Yield gains were attributed mainly to increased yield per plant, contributed by increases in kernel number per ear and 1000-kernel weight. The rates of lodging and barren plants of newer hybrids were significantly lower than those of older ones, especially at high plant density.

Traffic crashes occurring on long steep downgrades of highway is very frequent and severe in China. Crashes aggregate on some special places where black spots are formed in this kind of highway sections. Causes of traffic crashes black spots on long steep downgrade need to be analyzed in order to improve the traffic safety performance in those places. Based on the investigation of traffic crashes data of 5 years period occurring on a typical expressway in South China with long steep downgrades, the expressway infrastructure traffic safety performance was analyzed before and after the traffic safety countermeasures applied by crash aggregation analysis method. And based on a brake temperature transformation model of trucks, causes of traffic crashes black spots on the long steep downgrade of that expressway were analyzed. Overload was given as a main cause of those traffic crashes black spots. The findings were helpful to understanding the cause of the crashes deeply and defined the most useful traffic safety countermeasures on that kind of black spots.