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Abstract Substance Use Disorders (SUDs) present a significant challenge to global public health, with prolonged drug use not only impairing individual health but also hindering social development. Despite various interventions aimed at addressing drug abuse and dependence, a high relapse rate remains a prominent issue. In light of this, this study aims to explore the impact of perceived stress on the relapse of individuals with SUDs, as well as the mediating role of self-control and the moderating role of social support, in hopes of providing new perspectives for interventions to reduce the risk of relapse among individuals with SUDs. By utilizing a convenience sampling method, 420 male individuals with SUDs were recruited from detoxification centers in Guangxi, China. They completed questionnaires on perceived stress, self-control, social support, and tendencies towards relapse. A total of 401 valid datasets were obtained and analyzed using the SPSS Process plugin to conduct a moderated mediation model analysis. Results: (1) Perceived stress had a positive impact on the relapse of individuals with SUDs, (2) Self-control played a partial mediating role between perceived stress and the relapse, (3) The direct effect of perceived stress on the relapse and its first half of the indirect effect were moderated by social support. The research emphasize the critical importance of learning stress management strategies, enhancing self-control, and receiving comprehensive social support in the prevention and treatment of substance dependence. By strengthening self-control and social support as both internal and external resources, the likelihood of relapse among individuals with SUDs can be reduced, contributing to more effective and comprehensive drug rehabilitation strategies.

Abstract Background The number of spikelets per spike is a key trait that affects the yield of bread wheat (Triticum aestivum L.). Identification of the QTL for spikelets per spike and its genetic effects that could be used in molecular assistant breeding in the future. Results In this study, four recombinant inbred line (RIL) populations were generated and used, having YuPi branching wheat (YP), with Supernumerary Spikelets (SS) phenotype, as a common parent. QTL (QSS.sicau-2 A and QSS.sicau-2D) related to SS trait were mapped on chromosomes 2 A and 2D through bulked segregant exome sequencing (BSE-Seq). Fourteen molecular markers were further developed within the localization interval, and QSS.sicau-2 A was narrowed to 3.0 cM covering 7.6 Mb physical region of the reference genome, explaining 13.7 − 15.9% the phenotypic variance. Similarly, the QSS.sicau-2D was narrowed to 1.8 cM covering 2.4 Mb physical region of the reference genome, and it explained 27.4 − 32.9% the phenotypic variance. These two QTL were validated in three different genetic backgrounds using the linked markers. QSS.sicau-2 A was identified as WFZP-A, and QSS.sicau-2D was identified a novel locus, different to the previously identified WFZP-D. Based on the gene expression patterns, gene annotation and sequence analysis, TraesCS2D03G0260700 was predicted to be a potential candidate gene for QSS.sicau-2D. Conclusion Two significant QTL for SS, namely QSS.sicau-2 A and QSS.sicau-2D were identified in multiple environments were identified and their effect in diverse genetic populations was assessed. QSS.sicau-2D is a novel QTL associated with the SS trait, with TraesCS2D03G0260700 predicted as its candidate gene.

Background The aim of this study was to compare differences in shot placement and accuracy between national and international level squash players. Squash is a technically demanding sport and understanding the biomechanical characteristics of athletes at different levels is important for developing effective training strategies. Methods The study used a three-dimensional motion analysis system, a high-speed video camera, and a professional tee for biomechanical testing. Participants included national and international level squash players. The kinematic characteristics of the shoulder, elbow, and wrist joints of the upper extremity for backhand strokes, as well as the accuracy of the strokes, were analyzed to compare the differences between the two groups of athletes in terms of stroke posture and accuracy. Results The kinematic analysis of the backward backhand stroke revealed that national squash players showed significant differences compared to international players in several key parameters. Specifically, national players had significantly greater trunk flexion (P = 0.018) and less shoulder medial rotation (P = 0.027). They also had lower racket velocity in the X-direction (P = 0.043). However, there were no significant differences in trunk lateral flexion (P = 0.487), trunk rotation (P = 0.293), shoulder extension/flexion (P = 0.396), elbow flexion/extension (P = 0.818), wrist flexion/extension (P = 0.177), wrist rotation (P = 0.476), racket pitch (P = 0.112), racket velocity in the Y-direction (P = 0.587), or racket velocity in the Z-direction (P = 0.327). Additionally, data for racket yaw, racket roll, and racket Vx were not provided with significant values, indicating that these parameters do not show significant differences. Conclusion International level players outperformed national level players in squash stroke accuracy and control. Key kinematic factors influencing accuracy include trunk forward flexion, shoulder abduction, shoulder internal rotation, and racket angles. The study recommends that coaches design training to enhance technical details and positional control to improve squash performance.

Objective To investigate the impact of post-activation potentiation (PAP) induced by resisted sled sprint at different loads on the subsequent 30 m ice push sled performance of Chinese skeleton athletes, and to identify the resisted sled sprint load that most effectively enhances PAP for Chinese skeleton athletes. Methods Seven elite athletes from the Chinese skeleton team participated in four tests with more than 48 h intervals. During the tests, on the first test, athletes completed a 40 min standard warm-up, rested for 6 min, and then performed a 30 m test. On the second, third, and fourth test, athletes completed the standard warm-up, then performed 20 m sprints with resisted sled (RS) at 75%, 50%, and 25% of body mass (BM), respectively, rested for 6 min, and then performed the 30 m test. Results No significant differences were found in morning pulse, blood urea, and creatine kinase levels among four tests. The percentage of maximum heart rate (%HRmax) within different intensity ranges showed no significant differences among four tests. However, significant differences were observed in ice push sled performance among four tests (No BMRS: 5.08 ± 0.27; 25% BMRS: 5.05 ± 0.29; 50% BMRS: 5.02 ± 0.27; 75% BMRS: 5.04 ± 0.28). Post hoc analyses revealed that the 50% BMRS test had faster speed compared to the no resistance (p < 0.05), the 25% BMRS (p < 0.05), and the 75% BMRS (p < 0.05) tests. Additionally, the 75% BMRS test had faster speed than the no resistance test (p < 0.05). Conclusion A 20 m sprint with 50% BMRS effectively enhances the PAP effect in skeleton athletes, improving their ice push sled performance. Coaches can incorporate this resisted sled sprint in athletes’ training routines for performance enhancement in both daily training and pre-competition preparations.

Bicycle sharing has grown rapidly since the 2000s, but there is a lack of thorough retrospective analysis performed from a long-term perspective. This paper takes London as its focus, aiming to evaluate the performance of the London cycle hire (LCH) scheme, draw general practical implications for bicycle sharing, and summarize future research directions. This paper reviews the empirical evidence that has appeared in academic literature, policy documents, and technical reports. Issues covered in this review include: (1) LCH users and demand patterns, (2) substitutability and complementarity with other travel modes, (3) public health impacts, (4) interventions that have affected the usage and demand patterns of LCH, and (5) the impacts of COVID-19. Overall, LCH has achieved its primary goals of promoting cycling and has also brought benefits to public health and urban transportation resilience, and yet some minor problems persist. Practical implications for the implementation, operation, and evaluation of bicycle sharing schemes are offered based on our collection of evidence.

Abstract Cardiometabolic multimorbidity (CM), defined as the coexistence of two or three cardiometabolic disorders, is one of the most common and deleterious multimorbidities. This study aimed to investigate the association of Clínica Universidad de Navarra-Body Adiposity Estimator (CUN-BAE), body mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR) with the prevalence of CM. The data were obtained from the 2021 health checkup database for residents of the Electronic Health Management Center in Xinzheng, Henan Province, China. 81,532 participants aged ≥ 60 years were included in this study. Logistic regression models were used to estimate the odd ratios (ORs) and 95% confidence intervals (CIs) for CUN-BAE, BMI, WC, and WHtR in CM. The area under the receiver operating characteristic curve (AUC) was used to compare the discriminatory ability of different anthropometric indicators for CM. The multivariable-adjusted ORs (95% CIs) (per 1 SD increase) of CM were 1.799 (1.710–1.893) for CUN-BAE, 1.329 (1.295–1.364) for BMI, 1.343 (1.308–1.378) for WC, and 1.314 (1.280–1.349) for WHtR, respectively. Compared with BMI, WC and WHtR, CUN-BAE had the highest AUC in both males and females (AUC: 0.642; 95% CI 0.630–0.653 for males, AUC: 0.614; 95% CI 0.630–0.653 for females). CUN-BAE may be a better measure of the adverse effect of adiposity on the prevalence of CM than BMI, WC, and WHtR.

The potential of monitoring the movement of typhoons using the precipitable water vapor (PWV) has been confirmed. However, monitoring the movement of typhoon is focused on PWV, making it difficult to describe the movement of a typhoon in detail minutely and resulting in insufficient accuracy. Hence, based on PWV and meteorological data, we propose an improved typhoon monitoring mode. First, the European Centre for Medium-Range Weather Forecasts Reanalysis 5-derived PWV (ERA5-PWV) and the Global Navigation Satellite System-derived PWV (GNSS-PWV) were compared with the reference radiosonde PWV (RS-PWV). Then, using the PWV and atmospheric parameters derived from ERA5, we discussed the anomalous variations of PWV, pressure (P), precipitation, and wind speed during different typhoons. Finally, we compiled a list of critical factors related to typhoon movement, PWV and P. We developed an improved multi-factor typhoon monitoring mode (IMTM) with different models (i.e., IMTM-I and IMTM-II) in different cases with a higher density of GNSS observation or only Numerical Weather Prediction (NWP) data. The IMTM was evaluated through the reference movement speeds of HATO and Mangkhut from the China Meteorological Observatory Typhoon Network (CMOTN). The results show that the root mean square (RMS) of the IMTM-I is 1.26 km/h based on ERA5-P and ERA5-PWV, and the absolute bias values are mostly within 2 km/h. Compared with the models considering the single factor ERA5-P/ERA5-PWV, the RMS of the IMTM-I is improved by 26.3% and 38.5%, respectively. The IMTM-II model manifests a residual of only 0.35 km/h. Compared with the single-factor model based on GNSS-PWV/P, the residual of the IMTM-II model is reduced by 90.8% and 84.1%, respectively. These results propose that the typhoon movement monitoring approach combining PWV and P has evident advantages over the single-factor model and is expected to supplement traditional typhoon monitoring.

The construction of intelligent fully mechanized mining faces in China’s coal mines is in its early stages, and the automation technology of hydraulic support electro-hydraulic control has been widely applied. However, at present, the automation control logic of a single solidification of hydraulic supports is difficult to adapt to complex, ever-changing, and dynamic production scenarios. The actual control process in mine face still mostly adopts a collaborative approach of automation and manual intervention. In response to the demand for human-machine interaction and cooperation tasks in the complex scenarios of fully mechanized mining faces, a theoretical method and technical principle of a multimodal human-machine collaborative control system for hydraulic supports following the shearer in the middle range mining face (hereinafter referred to as hydraulic supports following) was proposed. Firstly, four human-machine collaborative modes, namely manual, division of labor, approval, and veto, were designed. Based on the factors such as coal seam geology, gas and dust, shearer speed, hydraulic support intelligence level, system status, job technical level, and task load, an AND-OR graph model for hydraulic support human-machine collaborative mode selection was constructed, which achieved a modal selection with manual or machine preferences. Then, a human-machine collaborative control decision-making mechanism for hydraulic supports following was designed. On this basis, an AI inference technology for the secondary control strategy of hydraulic supports following was proposed. Specifically, using on-site data to learn manual operation experience, a decision tree classification model for whether the hydraulic support should be secondary controlled and a Bayesian regression model for estimating the secondary control time of pulling hydraulic support were constructed. Based on the above model, a human-machine collaborative control decision-making program for the hydraulic support following was developed, which achieved human-machine collaborative control of hydraulic supports following based on AND/OR inference for modal selection and AI inference for secondary control strategy. Finally, using cloud edge end architecture software and hardware technology, a multimodal human-machine collaborative control system for hydraulic supports following was developed, which achieved the control functions such as model evolution, operational reasoning, and program execution. The system has undergone industrial trial operation on the 3404 mining face of Shaqu No.2 Mine. The result shows the efficiency of the hydraulic support following has increased by an average of 2% compared to that before. This paper forms an efficient and safe human-machine interactive decision-making mechanism for the comprehensive mining equipment group, which will provide practical theoretical methods and feasible technical paths for the development of intelligent comprehensive mining working faces.

Abstract Tai Chi serves as an effective exercise modality for enhancing autonomic regulation. However, a majority of existing studies have employed the single routine (SR) protocol as the basis for health interventions. The extent to which the gong routine application (GRA) protocol achieves similar levels of exercise load stimulation as traditional single practice routines remains uncertain. Therefore, this study the distinct characteristics of autonomic load stimulation in these different protocols, thus providing a biological foundation to support the development of Tai Chi health promotion intervention programs. we recruited a cohort of forty-five university students to participate in the 15 min GRA protocol and SR protocol. We collected heart rate and heart rate variability indicators during periods of rest, GRA protocol, and SR protocol utilizing the Polar Scale. Additionally, we assessed the mental state of the participants using the BFS State of Mind Scale. In summary, the autonomic load is lower in the GRA protocol compared to the SR protocol, with lower sympathetic activity but higher parasympathetic activity in the former. Results are specific to college students, additional research is necessary to extend support for frail older adults. It is advised to incorporate GRA protocol alongside SR protocol in Tai Chi instruction. This approach is likely to enhance Tai Chi skills and yield greater health benefits.

Streptococcus suis (S. suis) is an important swine bacterial pathogen and causes human infections, leading to a wide range of diseases. However, the role of 5’-nucleotidases in its virulence remains to be fully elucidated. Herein, we identified four cell wall-anchored 5’-nucleotidases (Snts) within S. suis, named SntA, SntB, SntC, and SntD, each displaying similar domains yet exhibiting low sequence homology. The malachite green reagent and HPLC assays demonstrated that these recombinant enzymes are capable of hydrolysing ATP, ADP, and AMP into adenosine (Ado), with the hierarchy of catalytic efficiency being SntC>SntB>SntA>SntD. Moreover, comprehensive enzymatic activity assays illustrated slight variances in substrate specificity, pH tolerance, and metal ion requirements, yet highlighted a conserved substrate-binding pocket, His–Asp catalytic dyad, metal, and phosphate-binding sites across Snts, with the exception of SntA. Through bactericidal assays and murine infection assays involving in site-mutagenesis strains, it was demonstrated that SntB and SntC collaboratively enhance bacterial survivability within whole blood and polymorphonuclear leukocytes (PMNs) via the Ado-A2aR pathway in vitro, and within murine blood and organs in vivo. This suggests a direct correlation between enzymatic activity and enhancement of bacterial survival and virulence. Collectively, S. suis 5’-nucleotidases additively contribute to the generation of adenosine, influencing susceptibility within blood and PMNs, and enhancing survival within blood and organs in vivo. This elucidation of their integral functions in the pathogenic process of S. suis not only enhances our comprehension of bacterial virulence mechanisms, but also illuminates new avenues for therapeutic intervention aimed at curbing S. suis infections.

ABSTRACTStreptococcus suis (S. suis), a significant zoonotic bacterial pathogen impacting swine and human, is associated with severe systemic diseases such as streptococcal toxic shock-like syndrome, meningitis, septicaemia, and abrupt fatality. The multifaceted roles of complement components C5a and C3a extend to orchestrating inflammatory cells recruitment, oxidative burst induction, and cytokines release. Despite the pivotal role of subtilisin-like serine proteases in S. suis pathogenicity, their involvement in immune evasion remains underexplored. In the present study, we identify two cell wall-anchored subtilisin-like serine proteases in S. suis, SspA-1 and SspA-2, as binding partners for C3a and C5a. Through Co-Immunoprecipitation, Enzyme-Linked Immunosorbent and Far-Western Blotting Assays, we validate their interactions with the aforementioned components. However, SspA-1 and SspA-2 have no cleavage activity against complement C3a and C5a performed by Cleavage assay. Chemotaxis assays reveal that recombinant SspA-1 and SspA-2 effectively attenuate monocyte chemotaxis towards C3a and C5a. Notably, the ΔsspA-1, ΔsspA-1, and ΔsspA-1/2 mutant strains exhibit compromised survival in blood, and resistance of opsonophagocytosis, alongside impaired survival in blood and in vivo colonization compared to the parental strain SC-19. Critical insights from the murine and Galleria mellonella larva infection models further underscore the significance of sspA-1 in altering mortality rates. Collectively, our findings indicate that SspA-1 and SspA-2 are novel binding proteins for C3a and C5a, thereby shedding light on their pivotal roles in S. suis immune evasion and the pathogenesis.

Background The objective of this study was to compare and analyze the representative opening and closing movement of Tai Chi elastic band exercise with the reverse fly movement of elastic band resistance training. The aim was to explore the biomechanical differences between the two exercises and provide theoretical support for the application of Tai Chi elastic band exercise in health intervention. Methods A total of 26 male participants were recruited and randomly divided into two groups in a 1:1 ratio. There were 13 participants in each Tai Chi elastic band exercise group and elastic band resistance training group. Both groups of participants used an elastic band to perform movement in the experiment. Experimental data were collected using the Vicon infrared motion capture system and Delsys surface EMG system. The AnyBody software was utilized to simulate the creation of a musculoskeletal model for both exercises. Result The study found that the Tai Chi elastic band exercise group exhibited smaller horizontal abduction angle and flexion angle of the shoulder joint, as well as normalized RMS of the anterior deltoid and triceps brachii, compared to the elastic band resistance training group (P < 0.01); the Tai Chi elastic band exercise group exhibited greater elbow flexion angle, elbow flexion torque, and muscle strength of the infraspinatus, coracobrachialis, biceps brachii, brachialis and brachioradialis, compared to the elastic band resistance training group (P < 0.01); the Tai Chi elastic band exercise group exhibited smaller horizontal abduction angular velocity of the shoulder joint and a lower normalized RMS of the posterior deltoid, compared to the elastic band resistance training group (P < 0.05). Conclusion (1) The opening and closing movement of Tai Chi elastic band exercise is characterized by a large elbow flexion angle, a small shoulder joint horizontal angle and flexion angle, and a slow and uniform speed of movement. The reverse fly movement of elastic band resistance training is characterized by a large horizontal abduction angle of the shoulder joint, a large flexion angle of the shoulder joint, a small flexion angle of the elbow joint, and a fast and uneven speed. (2) The opening and closing movement exerts a greater torque on the elbow flexion, while the reverse fly movement exerts a greater torque on the shoulder joint horizontal abduction and external rotation. (3) The opening and closing movement provide greater stimulation to the infraspinatus, coracobrachialis, and elbow flexor, while the reverse fly movement provides greater stimulation to the posterior deltoid, anterior deltoid, subscapularis, and elbow extensor. In summary, the variation in joint angle, joint angular velocity, and hand position could be the factor contributing to the differences in joint torque and muscle activity between the opening and closing movement of Tai Chi elastic band exercise and the reverse fly movement of elastic band resistance training.

BackgroundThe current lower limb robotic exoskeleton training (LRET) for treating and managing stroke patients remains a huge challenge. Comprehensive ICF analysis and informative treatment options are needed. This review aims to analyze LRET’ s efficacy for stroke patients, based on ICF, and explore the impact of intervention intensities, devices, and stroke phases.MethodsWe searched Web of Science, PubMed, and The Cochrane Library for RCTs on LRET for stroke patients. Two authors reviewed studies, extracted data, and assessed quality and bias. Standardized protocols were used. PEDro and ROB2 were employed for quality assessment. All analyses were done with RevMan 5.4.ResultsThirty-four randomized controlled trials (1,166 participants) were included. For function, LRET significantly improved motor control (MD = 1.15, 95%CI = 0.29–2.01, p = 0.009, FMA-LE), and gait parameters (MD = 0.09, 95%CI = 0.03–0.16, p = 0.004, Instrumented Gait Velocity; MD = 0.06, 95%CI = 0.02–0.09, p = 0.002, Step length; MD = 4.48, 95%CI = 0.32–8.65, p = 0.04, Cadence) compared with conventional rehabilitation. For activity, LRET significantly improved walking independence (MD = 0.25, 95%CI = 0.02–0.48, p = 0.03, FAC), Gait Velocity (MD = 0.07, 95%CI = 0.03–0.11, p = 0.001) and balance (MD = 2.34, 95%CI = 0.21–4.47, p = 0.03, BBS). For participation, social participation (MD = 0.12, 95%CI = 0.03–0.21, p = 0.01, EQ-5D) was superior to conventional rehabilitation. Based on subgroup analyses, LRET improved motor control (MD = 1.37, 95%CI = 0.47–2.27, p = 0.003, FMA-LE), gait parameters (MD = 0.08, 95%CI = 0.02–0.14, p = 0.006, Step length), Gait Velocity (MD = 0.11, 95%CI = 0.03–0.19, p = 0.005) and activities of daily living (MD = 2.77, 95%CI = 1.37–4.16, p = 0.0001, BI) for the subacute patients, while no significant improvement for the chronic patients. For exoskeleton devices, treadmill-based exoskeletons showed significant superiority for balance (MD = 4.81, 95%CI = 3.10–6.52, p

Brassica napus is one of the most important oil crops in the world. Breeding oilseed rape with colorful flowers can greatly enhance the ornamental value of B. napus and thus improve the economic benefits of planting. As water-soluble flavonoid secondary metabolites, anthocyanins are very important for the synthesis and accumulation of pigments in the petals of plants, giving them a wide range of bright colors. Despite the documentation of over 60 distinct flower shades in B. napus, the intricacies underlying flower color variation remain elusive. Particularly, the mechanisms driving color development across varying flower color backgrounds necessitate further comprehensive investigation. This research undertook a comprehensive exploration through the integration of transcriptome and metabolome analyses to pinpoint pivotal genes and metabolites underpinning an array of flower colors, including beige, beige-red, yellow, orange-red, deep orange-red, white, light-purple, and purple. First, we used a two-way BLAST search to find 275 genes in the reference genome of B. napus Darmor v10 that were involved in making anthocyanins. The subsequent scrutiny of RNA-seq outcomes underscored notable upregulation in the structural genes F3H and UGT, alongside the MYB75, GL3, and TTG1 transcriptional regulators within petals, showing anthocyanin accumulation. By synergizing this data with a weighted gene co-expression network analysis, we identified CHS, F3H, MYB75, MYB12, and MYB111 as the key players driving anthocyanin synthesis in beige-red, orange-red, deep orange-red, light-purple, and purple petals. By integrating transcriptome and weighted gene co-expression network analysis findings with anthocyanin metabolism data, it is hypothesized that the upregulation of MYB75, which, in turn, enhances F3H expression, plays a pivotal role in the development of pigmented oilseed rape flowers. These findings help to understand the transcriptional regulation of anthocyanin biosynthesis in B. napus and provide valuable genetic resources for breeding B. napus varieties with novel flower colors.

Dust deposited on the surface of snow and glaciers can significantly reduce the snow and ice albedo and accelerate melting. Manual observations of the dust mass concentration (DMC) on snow and glacier surfaces are routinely performed at many locations worldwide. However, snow and ice surface DMC monitoring methods based on remote sensing data still face challenges. This study presents a new retrieval scheme for estimating dust load on snow-covered surfaces from a moderate-resolution imaging spectroradiometer and visible infrared imaging radiometer suite in Northeast and Northwest China that utilizes a classical snow radiative transfer model. Our results indicate that the coefficient of variation of DMC retrieved from the in situ measurements of snow spectral reflectance is 4%, which is within a +4% difference compared with DMC observed in snow and ice samples. Estimating atmospheric dust pollutants content deposited on snow surfaces based on satellite remote sensing observations is feasible. In Northwest China, the root-mean-square error (RMSE) of the DMC values retrieved from VNP09GA data is 9.78 ppm, while that of the DMC values retrieved from MOD09GA data is 13.74 ppm. In Northeast China, the RMSE of the DMC values retrieved from VNP09GA data is 73.98 ppm, while that of the DMC values retrieved from MOD09GA data is 184.32 ppm. The research results can realize continuous monitoring of the atmospheric dust pollutants deposited on snow surfaces, which is of great practical significance to understanding and studying the pollution process of atmospheric dust on snow.

The purpose of this study was to analyze the lower-limb muscle synergies of young and older adults during stationary cycling across various mechanical conditions to reveal adaptive strategies employed by the elderly to address various common pedaling tasks and function degradation. By comparing lower-limb muscle synergies during stationary cycling between young and old people, this study examined changes in muscle synergy patterns during exercise in older individuals. This is crucial for understanding neuromuscular degeneration and changes in movement patterns in older individuals. Sixteen young and sixteen older experienced cyclists were recruited to perform stationary cycling tasks at two levels of power (60 and 100 W) and three cadences (40, 60, and 90 rpm) in random order. The lower-limb muscle synergies and their inter- and intra-individual variability were analyzed. Three synergies were extracted in this study under all riding conditions in both groups while satisfying overall variance accounted for (VAF) > 85% and muscle VAF > 75%. The older adults exhibited lower variability in synergy vector two and a higher trend in the variability of activation coefficient three, as determined by calculating the variance ratio. Further analyses of muscle synergy structures revealed increased weighting in major contribution muscles, the forward-shifting peak activation in synergy one, and lower peak magnitude in synergy three among older adults. To produce the same cycling power and cadence as younger individuals, older adults make adaptive adjustments in muscle control—increased weighting in major contribution muscles, greater consistency in the use of primary force-producing synergies, and earlier peak activation of subsequent synergy.

(1) Background: This study aims to compare the effects of AI-based exercise feedback and standard training on the physical and mental health outcomes of older adults participating in a 4-week tai chi training program. (2) Methods: Participants were divided into three groups: an AI feedback group received real-time movement accuracy feedback based on AI and inertial measurement units (IMUs), a conventional feedback group received verbal feedback from supervisors, and a control group received no feedback. All groups trained three times per week for 8 weeks. Outcome measures, including movement accuracy, balance, grip strength, quality of life, and depression, were assessed before and after the training period. (3) Results: Compared to pre-training, all three groups showed significant improvements in movement accuracy, grip strength, quality of life, and depression. Only the AI feedback group showed significant improvements in balance. In terms of movement accuracy and balance, the AI feedback group showed significantly greater improvement compared to the conventional feedback group and the control group. (4) Conclusions: Providing real-time AI-based movement feedback during tai chi training offers greater health benefits for older adults compared to standard training without feedback.

(1) Background: Exploring the relationship between spontaneous breathing patterns and aerobic and anerobic running exercise performance can greatly improve our understanding of optimizing physical fitness. Spontaneous breathing patterns refer to how the rhythm and depth of breathing affect performance and physical adaptation during exercise. (2) Methods: This study aimed to investigate this relationship by enrolling 240 college students (120 males and 120 females, aged 18–22). We evaluated their resting respiratory rate (RR), the combined total of abdominal and thoracic movements (AM+TM), the proportion of abdominal movement to the overall respiratory movement (AM/(AM+TM)), and the inhalation to exhalation ratio (I/E ratio). Additionally, their performance in a 50 m sprint (measuring anerobic capability), an 800- or 1000 m run (assessing mixed aerobic and anerobic capacity), and a 12 min distance run (evaluating aerobic capacity) was recorded. (3) Results: Our findings, through both correlational and comparative analyses, indicate that a larger AM+TM is predictive of a greater distance covered in the 12 min run, suggesting enhanced aerobic capacity. Interestingly, among female participants, a lower body mass index (BMI) coupled with a higher proportion of abdominal movement (AM/(AM+TM)) was linked to better performance in the 800 m run, indicative of superior mixed aerobic and anerobic capacities. These results imply that women with a larger tidal volume and those with a lower BMI but higher abdominal contribution to breathing at rest may exhibit better aerobic and mixed exercise capacities, respectively. (4) Conclusions: Based on these findings, we recommend that healthcare professionals and physical education instructors incorporate respiratory pattern assessments into their practices to potentially improve the physical health of their clients and students, with a particular emphasis on female populations. This study underscores the importance of understanding the intricate relationship between spontaneous respiratory patterns and exercise capabilities in enhancing overall physical fitness and health.

(1) Background: The aim of this study was to assess lower limb muscle strength in older adults during the transfer from sitting to standing (STS) using an inertial measurement unit (IMU). Muscle weakness in this population can severely impact function and independence in daily living and increase the risk of falls. By using an IMU, we quantified lower limb joint moments in the STS test to support health management and individualized rehabilitation program development for older adults. (2) Methods: This study involved 28 healthy older adults (13 males and 15 females) aged 60–70 years. The lower limb joint angles and moments estimated using the IMU were compared with a motion capture system (Mocap) (pair t-test, ICC, Spearman correlations, Bland–Altman plots) to verify the accuracy of the IMU in estimating lower limb muscle strength in the elderly. (3) Results: There was no significant difference in the lower limb joint angles and moments calculated by the two systems. Joint angles and moments were not significantly different (p > 0.05), and the accuracy and consistency of the IMU system was comparable to that of the Mocap system. For the hip, knee, and ankle joints, the ICCs for joint angles were 0.990, 0.989, and 0.885, and the ICCs for joint moments were 0.94, 0.92, and 0.89, respectively. In addition, the results of the two systems were highly correlated with each other: the r-values for hip, knee, and ankle joint angles were 0.99, 0.99, and 0.96, and the r-values for joint moments were 0.92, 0.96, and 0.85. In the present study, there was no significant difference (p > 0.05) between the IMU system and the Mocap system in calculating lower limb joint angles and moments. (4) Conclusions: This study confirms the accuracy of the IMU in assessing lower limb muscle strength in the elderly. It provides a portable and accurate alternative for the assessment of lower limb muscle strength in the elderly.

(1) Background: This study utilized a 24-week intervention incorporating heart rate sensors for real-time monitoring of intervention training, aiming to comprehensively assess the effects of Tai Chi on vascular endothelial function, atherosclerosis progression, and lipid metabolism. The insights gained may inform personalized non-pharmacological interventions to enhance the management of cardiovascular health in this population to provide sustainable benefits and improve quality of life. (2) Methods: Forty postmenopausal middle-aged and elderly women were randomly assigned to an exercise or control group. The exercise group underwent a 24-week Tai Chi (BaFa WuBu) training intervention with real-time heart rate monitoring using Polar sensors. Pre- and post-intervention assessments included body composition, blood pressure, vascularity, and blood parameters measured with the Inbody 720, Vascular Endothelial Function Detector, and Arteriosclerosis. Data were analyzed using SPSS 26.0 and mixed-design ANOVA to assess the effects of time, group, and their interactions on study outcomes. (3) Results: After training through 24 weeks of Tai Chi (BaFa WuBu) intervention, compared with the control group, systolic blood pressure in the exercise group was significantly lower (p < 0.05), and the difference between left and right arm pulse pressure, left and right ankle mean arterial pressure, left and right side baPWV, left and right side ABI, TC, TG, LDL, and blood pressure viscosity were all very significantly lower (p < 0.01), and the diastolic blood pressure was significantly higher (p < 0.05). Compared with baseline values in the exercise group, systolic blood pressure, right and left arm pulse pressure difference, right and left ankle mean arterial pressure, right and left side baPWV, right and left side ABI, TC, TG, LDL, and blood pressure viscosity decreased very significantly (p < 0.01) and diastolic blood pressure and FMD increased very significantly (p < 0.01) in the exercise group after the intervention. (4) Conclusions: In our study, a 24-week Tai Chi (BaFa WuBu) program significantly improved vascular health in middle-aged and older postmenopausal women. This simplified Tai Chi form is gentle and effective, ideal for older adults. Regular practice led to reduced vascular obstruction, improved lipid metabolism, and enhanced vascular endothelial function, crucial for preventing vascular diseases. The real-time heart rate sensors used were pivotal, enabling precise monitoring and adjustment of exercise intensity, thereby enhancing the study’s scientific rigor and supporting Tai Chi (BaFa WuBu) as a beneficial therapeutic exercise.

Airspeed measurement is crucial for UAV control. To achieve accurate airspeed measurements for UAVs, this paper calculates airspeed data by measuring changes in air pressure and temperature. Based on this, a data processing method based on mechanical filtering and the improved AR-SHAKF algorithm is proposed to indirectly measure airspeed with high precision. In particular, a mathematical model for an airspeed measurement system was established, and an installation method for the pressure sensor was designed to measure the total pressure, static pressure, and temperature. Secondly, the measurement principle of the sensor was analyzed, and a metal tube was installed to act as a mechanical filter, particularly in cases where the aircraft has a significant impact on the gas flow field. Furthermore, a time series model was used to establish the sensor state equation and the initial noise values. It also enhanced the Sage–Husa adaptive filter to analyze the unavoidable error impact of initial noise values. By constraining the range of measurement noise, it achieved adaptive noise estimation. To validate the superiority of the proposed method, a low-complexity airspeed measurement device based on MEMS pressure sensors was designed. The results demonstrate that the airspeed measurement device and the designed velocity measurement method can effectively calculate airspeed with high measurement accuracy and strong interference resistance.

Background Circular RNAs (circRNAs) are a large class of RNAs present in mammals. Among these, circCamsap1 is a well-acknowledged circRNA with significant implications, particularly in the development and progression of diverse tumors. However, the potential consequences of circCamsap1 depletion in vivo on male reproduction are yet to be thoroughly investigated. Methods The presence of circCamsap1 in the mouse testes was confirmed, and gene expression analysis was performed using reverse transcription quantitative polymerase chain reaction. CircCamsap1 knockout mice were generated utilizing the CRISPR/Cas9 system. Phenotypic analysis of both the testes and epididymis was conducted using histological and immunofluorescence staining. Additionally, fertility and sperm motility were assessed. Results Here, we successfully established a circCamsap1 knockout mouse model without affecting the expression of parental gene. Surprisingly, male mice lacking circCamsap1 (circCamsap1−/−) exhibited normal fertility, with no discernible differences in testicular and epididymal histology, spermatogenesis, sperm counts or sperm motility compared to circCamsap1+/+ mice. These findings suggest that circCamsap1 may not play an essential role in physiological spermatogenesis. Nonetheless, this result also underscores the complexity of circRNA function in male reproductive biology. Therefore, further research is necessary to elucidate the precise roles of other circRNAs in regulating male fertility.

BackgroundCoronary heart disease (CHD) is the leading cause of death in both developed and many developing countries. Exercise training is a fundamental component of cardiac rehabilitation programs for patients with CHD. This study aims to investigate the effects of a Tai Chi rehabilitation program, which is provided through a hybrid online and offline mode, on oxidative stress and inflammatory responses in patients with CHD.MethodsA total of 34 patients with coronary heart disease were randomly assigned to two groups: an experiment group (n = 14, age 62.07 ± 9.076 years) and a control group (n = 20, age 61.90 ± 9.700 years). The experiment group underwent a 12-week Tai Chi cardiac rehabilitation program (TCCRP), while the control group followed a conventional exercise rehabilitation program (CERP) consisting of 1-h sessions, 3 times per week, for a total of 36 sessions. Participants were studied at baseline and post-intervention. The main assessments include the levels of Malondialdehyde (MDA), Superoxide dismutase (SOD), Tumor necrosis factor (TNF-α) and Interleukin-10 (IL − 10) in blood samples. Pearson correlation analysis was used, and the differences between the two groups were subsequently tested using two-way repeated ANOVA. Statistical significance was defined as a two-sided p-value of

The outcome of AL amyloidosis remains poor, particularly in patients with advanced organ involvement which takes long time to recovery. We conducted an observational study of two patients with AL amyloidosis treated with SDd regimen. Both patients successfully achieved significant hematological and organ responses without severe adverse events, and the time to organ response was remarkably shorter than previously reported. Notably, an over 15% reduction in interventricular septal thickness (IVST) was observed in patient#2 within 6 months. Up to now, SDd therapy has not been previously reported in AL amyloidosis and may be a promising option for these patients.

Introduction The prevalence of atrial fibrillation (AF) is increasing globally, and stroke prevention is the key to reduce the morbidity and mortality related to AF. Currently, direct oral anticoagulants (DOACs) are the primary options for stroke prevention, while it increases risk of bleeding. Left atrial appendage (LAA) is suspected as a vital source of cerebral emboli and may lead to ischaemic stroke, and thoracoscopic LAA clipping procedure provides an alternative option for stroke prevention in high-risk patients. However, high-quality evidence comparing LAA clipping to DOACs in terms of stroke prevention is lacking. This trial is designed to assess whether the efficacy of thoracoscopic LAA clipping is superior to DOACs for stroke prevention in AF patients at high risk of thrombosis (CHA2DS2-VASc≥2 in men and ≥3 in women)[CHA2DS2-VASc stands for congestive heart failure, hypertension, age ≥75 (doubled), diabetes, stroke (doubled), vascular disease, age 65 to 74 and sex category (female)].Methods and analysis This is a prospective, multicentre, open-labelled, randomised controlled study. This trial will randomly assign 290 patients with non-paroxysmal AF to thoracoscopic LAA clipping group or DOAC therapy group in a 1:1 randomisation. The primary endpoint is defined as a composite endpoint event consisting of stroke, systemic embolism, all-cause mortality, major bleeding events and clinically relevant non-major bleeding events at 24 months after randomisation. The secondary endpoints consist of the components of the primary composite endpoint, surgery-related adverse events and minor bleeding events.Ethics and dissemination The central ethics committee at Fuwai Hospital approved the trial entitled Epicardial left atrial appendage clipping versus direct oral anticoagulant to reduce stroke risk in non-paroxysmal atrial fibrillation (LAA-CLIP trial). The results of this study will be disseminated through publications in peer-reviewed journals and conference presentations.Trial registration number NCT06021808.

Abstract Background The prevalence of obesity, hypertension and diabetes is increasing. Hypertension and diabetes are common complications. Additionally, obesity and hypertension–diabetes comorbidity (HDC) are both closely related to insulin resistance. The aim of this study was to determine the association of obesity indicators with HDC in elderly individuals. Methods This retrospective cohort study included 74,955 subjects aged ≥ 60 years living in Xinzheng, Henan Province, from January 2011 to December 2019. The data were collected from the annual health examination dataset. Cox proportional hazard regression models and competing-risk survival regression models were used to examine the relationships between the three indicators and HDC risk. Results After 346,504 person-years of follow-up, HDC developed in 9,647 subjects. After further adjustments for confounders and death competing risks, compared with a body mass index (BMI) of 18.5–23.9 kg/m2, the fully adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) of BMI

Abstract In pressurized water reactor (PWR), fretting wear is one of the main causes of fuel assembly failure. Moreover, the operation condition of cladding is complex and harsh. A unique fretting damage test equipment was developed and tested to simulate the fretting damage evolution process of cladding in the PWR environment. It can simulate the fretting wear experiment of PWR under different temperatures (maximum temperature is 350 ℃), displacement amplitude, vibration frequency, and normal force. The fretting wear behavior of Zr-4 alloy under different temperature environments was tested. In addition, the evolution of wear scar morphology, profile, and wear volume was studied using an optical microscope (OM), scanning electron microscopy (SEM), and a 3D white light interferometer. Results show that higher water temperature evidently decreased the cladding wear volume, the wear mechanism of Zr-4 cladding changed from abrasive wear to adhesive wear and the formation of an oxide layer on the wear scar reduced the wear volume and maximum wear depth.

This manuscript presents the results from the synthesis and characterization of a slag analogue with a nominal composition of 17 wt% LiMnO2 and 83 wt% Ca2SiO4 encountering fairly high cooling rates in order to study the evolution of Mn-species. The Mn species was also simulated from 1223 K to 1773 K using a thermodynamic model, assuming a homogeneous melt. The micro-composition including the Mn species of the solidified slag was determined experimentally, and was used as basis for molecular dynamics (MD) simulation. The MD simulation provides information on structure and viscosity at high temperatures, which are otherwise difficult to access. These parameters significantly influence oxidation state of redox-active elements and the solidified product. The micro-composition analyzed by electron probe micro analysis (EPMA) and synchrotron-based micro-X-ray fluorescence (micro-XRF) showed that Mn-rich and Ca-Si-rich phases are separated. While the Mn-O phases did not contain noticeable Ca, the Ca2SiO4 phase had incorporated 0.6 wt% of Mn. The slag solidified into round-shaped and droplet-shaped grains of a Li-Mn-oxide, some Mn3O4 and Ca2SiO4. The powder X-ray diffraction (PXRD) confirmed the formation of larnite; the identity of the Li-Mn-oxide, however, remained inconclusive. The Mn oxidation state (OS) was identified using synchrotron-based micro-X-ray absorption near edge spectroscopy (micro-XANES). The Mn-O grains matched well with Li-Mn-oxides and a Mn OS: +3, e.g., LiMn3+O2. Small areas matching hausmannite (Mn2+Mn23+O4) were also identified. The OS of Mn in the silicate phase could not be identified. For comparison, a slowly cooled slag analogue with similar composition, but higher Si content, was also subjected to micro-XANES. The slowly cooled slag formed long Mn-rich needles in a matrix of large calcium silicate crystals. The Mn-rich crystals matched well with the XANES spectrum of a Mn3+ Li-oxide like LiMn3+O2. At the rim of the needles, the Mn-spectra matched well the hausmannite (Mn2+Mn23+O4) reference. In the silicate phases, Mn had an OS: +2, unambiguously. The melt structure at different temperatures of two compositions, i.e., LiMn3+O2 and Ca2SiO4, was simulated using molecular dynamics (MD). They serve as model compositions, assuming a heterogeneous melt. The results show significant different degrees of polymerization and viscosity. Information from MD simulations can support the identification of potentially different oxygen permeability and with that prediction of oxidation states. The bulk composition was identified by inductively coupled plasma optical emission spectrometry (ICP-OES), bulk structure by PXRD and bulk species by lab-XANES. The synchrotron micro analysis including micro-XRD were performed at the microfocus beamline I18 at the Diamond Light Source. Pure reference compounds were prepared and characterized with the same multi-modal approach.

Oilseed rape (Brassica napus) is a crucial global oil crop. It is generally cultivated in rotation with rice in southern China’s Yangtze River Basin, where the wet soil and residue retention after rice harvest significantly hinder its seedling establishment. Hence, this study developed a strip-tillage seeder for oilseed rape seeding following rice harvest. Additionally, seedling establishment, soil infiltration and evaporation post-seeding, soil moisture change, oilseed yield, and weed occurrence under strip tillage (ST) were compared with conventional shallow rotary-tillage (SR) and deep rotary-tillage (DR) seeding practices. Compared to SR and DR, the results demonstrated that ST had a higher seeding efficiency and 53.8% and 80.2% lower energy consumption, respectively. ST also enhanced seedling growth and oilseed yield formation more effectively than the competitor tillage treatments, with an oilseed yield increase exceeding 6%. Additionally, ST improved water infiltration and reduced soil water evaporation, resulting in higher topsoil (0–20 cm) moisture during the critical growth stages. Furthermore, ST reduced soil disturbance, significantly decreasing the density of the dominant weed, Polypogon fugax. Overall, ST seeding technology has the potential to improve the productivity of oilseed rape in rice–oilseed rape rotation systems, and its yield superiority is mainly due to seedling establishment improvement and soil moisture adjustment.

Point cloud registration is a crucial technique in photogrammetry, remote sensing, etc. A generalized 3D point cloud registration framework has been developed to estimate the optimal rigid transformation between two point clouds using 3D key point correspondences. However, challenges arise due to the uncertainty in 3D key point detection techniques and the similarity of local surface features. These factors often lead to feature descriptors establishing correspondences containing significant outliers. Current point cloud registration algorithms are typically hindered by these outliers, affecting both their efficiency and accuracy. In this paper, we propose a fast and robust point cloud registration method based on a compatibility graph and accelerated guided sampling. By constructing a compatible graph with correspondences, a minimum subset sampling method combining compatible edge sampling and compatible vertex sampling is proposed to reduce the influence of outliers on the estimation of the registration parameters. Additionally, an accelerated guided sampling strategy based on preference scores is presented, which effectively utilizes model parameters generated during the iterative process to guide the sampling toward inliers, thereby enhancing computational efficiency and the probability of estimating optimal parameters. Experiments are carried out on both synthetic and real-world data. The experimental results demonstrate that our proposed algorithm achieves a significant balance between registration accuracy and efficiency compared to state-of-the-art registration algorithms such as RANSIC and GROR. Even with up to 2000 initial correspondences and an outlier ratio of 99%, our algorithm achieves a minimum rotation error of 0.737° and a minimum translation error of 0.0201 m, completing the registration process within 1 s.

(1) Background: The objective of this study was to predict the vascular health status of elderly women during exercise using pulse wave data and Temporal Convolutional Neural Networks (TCN); (2) Methods: A total of 492 healthy elderly women aged 60–75 years were recruited for the study. The study utilized a cross-sectional design. Vascular endothelial function was assessed non-invasively using Flow-Mediated Dilation (FMD). Pulse wave characteristics were quantified using photoplethysmography (PPG) sensors, and motion-induced noise in the PPG signals was mitigated through the application of a recursive least squares (RLS) adaptive filtering algorithm. A fixed-load cycling exercise protocol was employed. A TCN was constructed to classify flow-mediated dilation (FMD) into “optimal”, “impaired”, and “at risk” levels; (3) Results: TCN achieved an average accuracy of 79.3%, 84.8%, and 83.2% in predicting FMD at the “optimal”, “impaired”, and “at risk” levels, respectively. The results of the analysis of variance (ANOVA) comparison demonstrated that the accuracy of the TCN in predicting FMD at the impaired and at-risk levels was significantly higher than that of Long Short-Term Memory (LSTM) networks and Random Forest algorithms; (4) Conclusions: The use of pulse wave data during exercise combined with the TCN for predicting the vascular health status of elderly women demonstrated high accuracy, particularly in predicting impaired and at-risk FMD levels. This indicates that the integration of exercise pulse wave data with TCN can serve as an effective tool for the assessment and monitoring of the vascular health of elderly women.

(1) Background: The objective of this study was to recognize tai chi movements using inertial measurement units (IMUs) and temporal convolutional neural networks (TCNs) and to provide precise interventions for elderly people. (2) Methods: This study consisted of two parts: firstly, 70 skilled tai chi practitioners were used for movement recognition; secondly, 60 elderly males were used for an intervention study. IMU data were collected from skilled tai chi practitioners performing Bafa Wubu, and TCN models were constructed and trained to classify these movements. Elderly participants were divided into a precision intervention group and a standard intervention group, with the former receiving weekly real-time IMU feedback. Outcomes measured included balance, grip strength, quality of life, and depression. (3) Results: The TCN model demonstrated high accuracy in identifying tai chi movements, with percentages ranging from 82.6% to 94.4%. After eight weeks of intervention, both groups showed significant improvements in grip strength, quality of life, and depression. However, only the precision intervention group showed a significant increase in balance and higher post-intervention scores compared to the standard intervention group. (4) Conclusions: This study successfully employed IMU and TCN to identify Tai Chi movements and provide targeted feedback to older participants. Real-time IMU feedback can enhance health outcome indicators in elderly males.

(1) Background: This study aims to investigate the correlation between heart rate variability (HRV) during exercise and recovery periods and the levels of anxiety and depression among college students. Additionally, the study assesses the accuracy of a multilayer perceptron-based HRV analysis in predicting these emotional states. (2) Methods: A total of 845 healthy college students, aged between 18 and 22, participated in the study. Participants completed self-assessment scales for anxiety and depression (SAS and PHQ-9). HRV data were collected during exercise and for a 5-min period post-exercise. The multilayer perceptron neural network model, which included several branches with identical configurations, was employed for data processing. (3) Results: Through a 5-fold cross-validation approach, the average accuracy of HRV in predicting anxiety levels was 89.3% for no anxiety, 83.6% for mild anxiety, and 74.9% for moderate to severe anxiety. For depression levels, the average accuracy was 90.1% for no depression, 84.2% for mild depression, and 82.1% for moderate to severe depression. The predictive R-squared values for anxiety and depression scores were 0.62 and 0.41, respectively. (4) Conclusions: The study demonstrated that HRV during exercise and recovery in college students can effectively predict levels of anxiety and depression. However, the accuracy of score prediction requires further improvement. HRV related to exercise can serve as a non-invasive biomarker for assessing psychological health.

The present study aimed to investigate the impacts of dietary standardized ileal digestible lysine to net energy (SID Lys:NE) ratio on lipid metabolism in pigs fed high-wheat diets. Thirty-six crossbred growing barrows (65.20 ± 0.38 kg) were blocked into two treatment groups, fed high-wheat diets with either a high SID Lys:NE ratio (HR) or a low SID Lys:NE ratio (LR). Each treatment group consisted of three replicates, with six pigs per pen in each replicate. The diminishing dietary SID Lys:NE ratio exhibited no adverse impacts on the carcass trait (p > 0.05) but increased the marbling score of the longissimus dorsi muscle (p < 0.05). Meanwhile, LR diets tended to increase the serum triglyceride concentration (p < 0.1). LR diets upregulated fatty acid transport protein 4 and acetyl-coA carboxylase α expression levels and downregulated the expression level of adipose triglyceride lipase (p < 0.05). LR diets improved energy metabolism via decreasing the expression levels of AMP-activated protein kinase (AMPK) α1, sirtuin 1 (SIRT1), and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) (p < 0.05). Additionally, LR diets stimulated hepatic bile acid synthesis via upregulating the expression levels of cytochrome P450 family 7 subfamily A member 1 and cytochrome P450 family 27 subfamily A member 1, and downregulating farnesol X receptor (FXR) and small heterodimer partner (SHP) expression levels (p < 0.05). A lowered SID Lys:NE ratio affected the colonic microbial composition, characterized by increased relative abundances of YRC22, Parabacteroides, Sphaerochaeta, and Bacteroides, alongside a decreased in the proportion of Roseburia, f_Lachnospiraceae_g_Clostridium, Enterococcus, Shuttleworthia, Exiguobacterium, Corynebacterium, Subdoligranulum, Sulfurospirillum, and Marinobacter (p < 0.05). The alterations in microbial composition were accompanied by a decrease in colonic butyrate concentration (p < 0.1). The metabolomic analysis revealed that LR diets affected primary bile acid synthesis and AMPK signaling pathway (p < 0.05). And the mantel analysis indicated that Parabacteroides, Sphaerochaeta, f_Lachnospiraceae_g_Clostridium, Shuttleworthia, and Marinobacter contributed to the alterations in body metabolism. A reduced dietary SID Lys:NE ratio improves energy metabolism, stimulates lipogenesis, and inhibits lipolysis in finishing pigs by regulating the AMPKα/SIRT1/PGC-1α pathway and the FXR/SHP pathway. Parabacteroides and Sphaerochaeta benefited bile acids synthesis, whereas f_Lachnospiraceae_g_Clostridium, Shuttleworthia, and Marinobacter may contribute to the activation of the AMPK signaling pathway. Overall, body metabolism and colonic microbiota collectively controlled the lipid metabolism in finishing pigs.

The three spacecraft of the space gravitational wave antenna employ heterodyne interferometry to mitigate the effects of Doppler shift. Constrained by laser relative intensity noise (RIN) and the sampling frequency constraints of phase readout circuits, the widespread adoption of fixed offset frequencies effectively regulates the frequency of heterodyne interferometric beat notes within a reasonable frequency domain of [5 MHz, 25 MHz]. In this work, a high-precision fitness genetic algorithm for heterodyne interferometry is utilized to generate the initial offset frequency distribution scheme. To address issues with unreasonable switching times and offset frequency settings in the initial scheme for partial frequency domains, optimization strategies are proposed from three aspects: frequency domain selection extension, switch times control, and numerical low frequency. Results demonstrate that the optimization of frequency domain selection extension narrows the reasonable frequency domain to [5 MHz, 15 MHz] and [7 MHz, 17 MHz]. Optimization of switch times control ensures that switching times of offset frequency distribution scheme generated under the settings of [6 MHz, 17 MHz] and wider frequency domains can be controlled within a reasonable range of 6 to 13 times. Fixed offset frequency settings are generally reduced by 24.3% after low-frequency optimization. This methodology and result can provide a reliable reference for Program Taiji and even related space gravitational wave antenna projects.

The alkali-activated composites technique is a promising method for the in situ preparation of cavity filling/grouting materials from engineering waste soil. To investigate the feasibility of engineering waste soil utilization by the alkali activation process, the macroscopic and microscopic properties of the fly ash/slag-based alkali-activated composites, after solidification/stabilization (S/S) with sandy clay excavated at Baishitang Station of Shenzhen Metro, were studied. The unconfined compressive strength (UCS) test was conducted to evaluate the S/S effect of alkali-activated composites. The results show that the optimum quality ratio of slag and fly ash correspond to 7:3, the modulus of alkaline activator to 1.3, and the alkalinity of alkaline activator to 10%. The alkali-activated composite’s strength under these parameters can reach 45.25 MPa at 3 days, 49.85 MPa at 7 days, and 62.33 MPa at 28 days. A maximum 3-day UCS of 21.71 MPa, 75% of the 28-day UCS, was achieved by an engineering waste soil and alkali-activated composites mass ratio of 5:5, slaked lime content of 4.5%, and a water-to-solid ratio of 0.26, and it can also meet the required fluidity and setting time for construction well. Fluidity is primarily affected by the soil-to-binder ratio, which decreases as the ratio decreases, while the water-to-solid ratio increases fluidity. Slaked lime has minimal impact on fluidity. The setting time is mainly influenced by the soil-to-binder ratio, followed by slaked lime content and water-to-solid ratio, with setting time shortening as the soil-to-binder ratio and slaked lime content increase, and lengthening as the water-to-solid ratio increases. Through Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive Spectroscopy (EDS) tests, microscopic analysis showed that loose granular units are firmly cemented by alkali-activated composites. Based on the results of on-site grouting tests in karst caves, the alkali-activated grout materials reached a strength of 5.2 MPa 28 days after filling, which is 162.5% of the strength of cement grouting material, satisfying most of the requirements for cavity filling in Shenzhen.

Abstract Background Ankylosing spondylitis (AS) is a chronic inflammatory arthritis. Upregulation of microRNA (miR)-92b-3p is associated with enhanced osteoblastic differentiation. The current study sought to investigate the functional mechanism of miR-92b-3p in osteogenic differentiation of AS fibroblasts. Methods First, fibroblasts were isolated from AS and non-AS patients and cultured. Next, cell morphology was observed, cell proliferation was assessed and the vimentin expression pattern was determined. Alkaline phosphatase (ALP) activity and levels of osteogenic markers RUNX2, OPN, OSX, and COL I were additionally measured, followed by determination of miR-92b-3p and TOB1 levels. The binding site of miR-92b-3p and TOB1 was predicted, and their target relationship was validated. Lastly, miR-92b-3p inhibitor, si-TOB1, and the BMP/Smad signaling pathway inhibitor LDN193189 were delivered into AS fibroblasts to evaluate the osteogenic differentiation of AS fibroblasts and the activation of the BMP/Smad pathway. Results miR-92b-3p was highly expressed in AS fibroblasts. AS fibroblasts showed enhanced osteogenic differentiation and proliferation, while inhibition of miR-92b-3p suppressed osteogenic differentiation and proliferation of AS fibroblasts. miR-92b-3p targeted TOB1, and TOB1 was poorly expressed in AS fibroblasts. The concurrent downregulation of TOB1 and inhibition of miR-92b-3p elevated the levels of RUNX2, OPN, OSX, and COL I and ALP activity and further enhanced the proliferation of AS fibroblasts. The BMP/Smad pathway was activated in AS fibroblasts. Silencing miR-92b-3p could inhibit the activation of the BMP/Smad pathway by upregulating TOB1. Inhibition of the BMP/Smad pathway reduced the number of calcified nodules and hindered the osteogenic differentiation and proliferation of AS fibroblasts. Conclusion Our findings highlighted that silencing miR-92b-3p inhibited the osteogenic differentiation and proliferation of AS fibroblasts by upregulation of TOB1 and inhibition of the BMP/Smad pathway. Graphical abstract

To analyze the pipeline response under permanent ground deformation, the evolution of resistance acting on the pipe during the vertical downward offset is an essential ingredient. However, the efficient simulation of pipe penetration into soil is challenging for the conventional finite element (FE) method due to the large deformation of the surrounding soils. In this study, the B-spline material point method (MPM) is employed to investigate the pipe-soil interaction during the downward movement of rigid pipes buried in medium and dense sand. To describe the density- and stress-dependent behaviors of sand, the J2-deformation type model with state-dependent dilatancy is adopted. The effectiveness of the model is demonstrated by element tests and biaxial compression tests. Afterwards, the pipe penetration process is simulated, and the numerical outcomes are compared with the physical model tests. The effects of pipe size and burial depth are investigated with an emphasis on the mobilization of the soil resistance and the failure mechanisms. The simulation results indicate that the bearing capacity formulas given in the guidelines can provide essentially reasonable estimates for the ultimate force acting on buried pipes, and the recommended value of yield displacement may be underestimated to a certain extent.

Background: According to statistics, about one-fifth of the world's elderly people suffer from sleep disorders, and the problem of sleep disorders in the elderly is extremely serious, and this problem is one of the important causes of chronic diseases such as hypertension, hyperlipidemia, diabetes mellitus, and coronary heart disease in the elderly. The positive effect of Tai Chi exercise therapy on sleep problems has been confirmed, but at present, the effect of the specific duration of Tai Chi exercise on the improvement of elderly people with moderate to severe sleep disorders varies. Objective: META analysis was used to investigate and find that long-term Tai Chi exercise therapy has the best effect on improving sleep in elderly patients with moderate to severe sleep disorders. Methods: META analysis was performed using Revman 5.3 after searching Web of science, Pubmed, Scopus, The Cochroae Library, OVID, CBM, CNKI, VIP, and other databases, and then filtering and extracting. Results: A total of seven papers were included. Meta-analysis showed that tai chi exercise was more effective in improving sleep problems in elderly patients with sleep disorders compared to the control group, and the difference was significant. This was demonstrated by a decrease in the global Pittsburgh Sleep Quality Index (PSQI) score [SMD = −0.66, 95 % CI (−0.91, −0.41), P

Dopamine (DA) and uric acid (UA) are essential for many physiological processes in the human body. Abnormal levels of DA and UA can lead to multiple diseases, such as Parkinson’s disease and gout. In this work, a three-dimensional reduced graphene oxide–MXene (3D rGO-Ti3C2) composite electrode was prepared using a simple one-step hydrothermal reduction process, which could separate the oxidation potentials of DA and UA, enabling the simultaneous detection of DA and UA. The 3D rGO-Ti3C2 electrode exhibited excellent electrocatalytic activity towards both DA and UA. In 0.01 M PBS solution, the linear range of DA was 0.5–500 µM with a sensitivity of 0.74 µA·µM−1·cm−2 and a detection limit of 0.056 µM (S/N = 3), while the linear range of UA was 0.5–60 µM and 80–450 µM, with sensitivity of 2.96 and 0.81 µA·µM−1·cm−2, respectively, and a detection limit of 0.086 µM (S/N = 3). In 10% fetal bovine serum (FBS) solution, the linear range of DA was 0.5–500 µM with a sensitivity of 0.41 µA·µM−1·cm−2 and a detection limit of 0.091 µM (S/N = 3). The linear range of UA was 2–500 µM with a sensitivity of 0.11 µA·µM−1·cm−2 and a detection limit of 0.6 µM (S/N = 3). The modified electrode exhibited advantages such as high sensitivity, a strong anti-interference capability, and good repeatability. Furthermore, the modified electrode was successfully used for DA measurement in vivo. This could present a simple reliable route for neurotransmitter detection in neuroscience.

BackgroundStroke, which is a common clinical cerebrovascular disease, causes approximately 83% of survivors to suffer from balance impairments. Balance and gait training (BGT) is widely used to restore balance in patients with stroke. However, its wide variety presents clinicians with a dilemma when selecting interventions. This study aimed to compare and rank BGT interventions by quantifying information based on randomized controlled trials (RCTs).MethodsWe conducted a network meta-analysis (NMA) of non-gait-trained controls and head-to-head RCTs and compared the effects of 12 BGT interventions. A total of nine literature databases, including Medline, Embase, Cochrane Library, Web of Science, Scopus, SPORTDiscus, ClinicalTrials.gov, CNKI, and Chinese biomedical literature databases, were searched from their database inception to August 2023. Two authors independently selected studies and extracted data. The difference in outcomes, which were expressed as standardized mean differences and confidence intervals (CIs) of 95%, were explored in this meta-analysis.ResultsA total of 66 studies with 1,933 participants were included. Effect size estimates showed that not all BGT interventions were more effective than controls, with treadmill training as the least effective for balance test batteries (SMD = −0.41, 95% CI [−1.09, 0.27]) and proactive balance (SMD = −0.50, 95% CI [−1.14, 0.14]). Body-weight-supported treadmill training with external stimulation was most effective for proactive balance and dynamic steady-state balance (SMD = 1.57, 95% CI [−0.03, 3.16]); SMD = 1.18, 95% CI [0.67, 1.68]. Virtual reality gait training (SMD = 1.37, 95% CI [0.62, 2.11]) had the best effect on improving balance test batteries, while dual-task BGT (SMD = 1.64, 95% CI [0.50, 2.78]) had the best effect on static steady-state balance. After analyses for possible impact covariates, the findings through the outcomes did not change substantially. Confidence in the evidence was generally low or very low.ConclusionThis NMA suggested that virtual reality gait training was the most effective BGT modality for improving balance test batteries. Body-weight support treadmill training with external stimulation was the most effective for improving active and dynamic balance. In addition, dual-task BGT was the best choice for improving static balance. However, balance is a multidimensional concept, and patients’ different needs should be considered when selecting BGT.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022316057, ID: CRD42022316057.

ObjectiveTo explore the additional value of cardiac magnetic resonance (CMR) post-contrast T1 mapping in the detection of myocardial infarction, compared with late gadolinium enhancement (LGE).Materials and methodsA CMR database of consecutive patients with myocardial infarction was retrospectively analyzed. All patients were scanned at 3 T magnetic resonance; they underwent conventional CMR (including LGE) and post-contrast T1 mapping imaging. Two radiologists interpreted the CMR images using a 16-segment model. The first interpretation included only LGE images. After 30 days, the same radiologists performed a second analysis of random LGE images, with the addition of post-contrast T1 mapping images. Images were analyzed to diagnose myocardial scars, and the transmural extent of each scar was visually evaluated. Diagnoses retained after LGE were compared with diagnoses retained after the addition of post-contrast T1 mapping.ResultsIn total, 80 patients (1,280 myocardial segments) were included in the final analysis. After the addition of post-contrast T1 mapping, eight previously unidentified subendocardial scars were detected. Compared with LGE images, the percentage of infarcted segments was higher after the addition of post-contrast T1 mapping images (21.7% vs. 22.3%, P = 0.008), the percentage of uncertain segments was lower after the addition of post-contrast T1 mapping (0.8% vs. 0.1%, P = 0.004), and the percentage of uncertain transmural extent of scarring was lower after the addition of post-contrast T1 mapping (0.9% vs. 0.1%, P = 0.001).ConclusionThe addition of post-contrast T1 mapping after LGE helps to improve the detection of myocardial infarction, as well as the assessment of the transmural extent of scarring.

IntroductionMetastatic urothelial carcinoma (mUC) has poor prognosis. A high unmet need exists for novel treatment for those who are unfit for platinum-based chemotherapy.MethodsWe aimed to describe real-world temporal changes in patient characteristics and 1L treatment selection for mUC patients in the United States following the approval of anti-PD-1/L1 treatments. This study was a retrospective, observational study using anonymized and structured oncology electronic medical record (EMR) data from IQVIA and the US Oncology Network iKnowMed (USON).ResultsAfter approval of 1L anti-PD-1/L1 treatment for mUC, there is a marked increase in the use of 1L anti-PD-1/L1 monotherapies, accompanied by a proportional decrease in 1L platinum-based treatments and non-guideline-based therapy; particularly among the elderly (> 75 years) and those with poor ECOG performance status (ECOG PS 2+).DiscussionAnti-PD-1/L1 monotherapies fulfill the prior unmet need of frail mUC patients who are ineligible for platinum-based therapies.

Objectives: Clínica Universidad de Navarra-Body Adiposity Estimator (CUN-BAE) is considered to be a more accurate indicator of body fat estimation. We aimed to investigate the association of CUN-BAE with the risk of type 2 diabetes mellitus (T2DM) and to compare the strength of the association between CUN-BAE, body mass index (BMI), waist circumference (WC), waist-to-height ratio (WHtR) and T2DM.Methods: The data were obtained from the annual health checkup database of residents in Xinzheng, China. From January 2011 to December 2021, 80,555 subjects aged ≥45 years met the inclusion criteria. Cox proportional hazard regression models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs) for CUN-BAE, BMI, WC, and WHtR in T2DM.Results: During a mean follow-up of 6.26 years, T2DM occurred in 12,967 subjects. The multivariable-adjusted HRs (95% CIs) of T2DM (highest vs. reference group) were 1.994 (1.811–2.196) for CUN-BAE, 1.751 (1.665–1.842) for WC, 1.715 (1.631–1.804) for WHtR, and 1.510 (1.436–1.588) for BMI, respectively. In addition, the risk of T2DM increased with baseline CUN-BAE (HR: 1.374; 95% CI: 1.328, 1.421), WC (HR: 1.236; 95% CI: 1.215, 1.256), WHtR (HR: 1.228; 95% CI: 1.208, 1.248), and BMI (HR: 1.175; 95% CI: 1.156, 1.195).Conclusion: Compared to BMI, WC or WHtR, CUN-BAE may more adequately reflect the adverse effects of adiposity on the risk of T2DM.

To determine the overload characteristics of the internal system of a fuze that penetrates multilayer hard targets using different fixed-link structures, a finite element model consisting of two fixed-link structures (a compression screw and a body screw) is adopted in this paper to simulate the penetration process of a three-layer concrete target plate with corresponding initial velocities. The peak amplification coefficient and vibration coefficient are used to analyze the time-domain characteristics of the penetration process signal during segmented analysis. The extracted acceleration signals of the projectile and sensor are processed by fast Fourier transform to obtain the frequency spectrum analysis results. The simulation results show that under the same working conditions, the sensor’s ability to amplify the peak acceleration of the projectile is 17.77% higher for the body screw fixed-link structure, and the average vibration coefficient is also 9.55% higher. Compared with that of the body screw fixed-link structure, the performance of the compression screw fixed-link structure is better under different initial velocity conditions. The initial penetration velocity affects mainly the amplitude of each frequency corresponding to the acceleration signals of the two fixed-link fuze structure projectiles and sensors while having a relatively small influence on the frequency distribution position.

Background & Aims: Hepatoblastoma (HB) is a common pediatric malignant liver tumor that is characterized by a low level of genetic mutations. Alternative splicing (AS) has been shown to be closely associated with cancer progression, especially in tumors with a low mutational burden. However, the role of AS in HB remains unknown. Methods: Transcriptome sequencing was performed on 5 pairs of HB tissues and matched non-tumor tissues to delineate the AS landscape in HB. AS events were validated in 92 samples from 46 patients. RNA pull-down and RNA immunoprecipitation assays were carried out to identify splicing factors that regulate the AS of small nucleolar RNA host genes (SNHG). Patient-derived organoids (PDOs) were established to investigate the role of the splicing factor polyadenylate-binding nuclear protein 1 (PABPN1). Results: This study uncovered aberrant alternative splicing in HB, including lncRNAs from SNHG family that undergo intron retention in HB. Further investigations revealed that PABPN1, a significantly upregulated RNA binding protein, interacts with splicing machinery in HB, inducing the intron retention of these SNHG RNAs and the downregulation of intronic small nucleolar RNAs (snoRNAs). Functionally, PABPN1 acts as an oncofetal splicing regulator in HB by promoting cell proliferation and DNA damage repair via inducing the intron retention of SNHG19. Knock-down of PABPN1 increases the cisplatin sensitivity of HB PDOs. Conclusions: Our findings revealed the role of intron retention in regulating snoRNA expression in hepatoblastoma, explained detailed regulatory mechanism between PABPN1 and the intron retention of SNHG RNAs, and provided insight into the development of new HB treatment options.

Abstract Objective The reprogramming of metabolism is an important factor in the metastatic process of cancer. In our study, we intended to investigate the predictive value of metabolism-related genes (MRGs) in recurrent gastric cancer (GC) patients with peritoneal metastasis. Methods The sequencing data of mRNA of GC patients were obtained from Asian Cancer Research Group (ACRG) and the GEO databases (GSE53276). The differentially expressed MRGs (DE-MRGs) between a cell line without peritoneal metastasis (HSC60) and one with peritoneal metastasis (60As6) were analyzed with the Limma package. According to the LASSO regression, eight MRGs were identified as crucially related to peritoneal seeding recurrence in patients. Then, disease free survival related genes were screened using Cox regression, and a promising prognostic model was constructed based on 8 MRGs. We trained and verified it in two independent cohort. Results We confirmed 713 DE-MRGs and the enriched pathways. Pathway analysis found that the MRG-related pathways were related to tumor metabolism development. With the help of Kaplan–Meier analysis, we found that the group with higher risk scores had worse rates of peritoneal seeding recurrence than the group with lower scores in the cohorts. Conclusions This study developed an eight-gene signature correlated with metabolism that could predict peritoneal seeding recurrence for GC patients. This signature could be a promising prognostic model, providing better strategy in treatment.

Surgery is an important part of treatment for gastric adenocarcinoma, however, the consensus on perioperative care is less clear. Here, the authors report the results of a phase II clinical trial investigating neoadjuvant camrelizumab with concurrent chemoradiotherapy, followed by surgery for the treatment of advanced gastric adenocarcinoma.