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2. Genetic analysis of dioxin dioxygenase of Sphingomonas sp. strain RW1: catabolic genes dispersed on the genome

Author

Armengaud, Jean, Happe, Birgitta, and Timmis, Kenneth N.

Subjects
Micropollutants -- Research, Xenobiotics -- Research, Bacterial proteins -- Genetic aspects, Biological sciences
Abstract

Previous experiments resulted in the cloning of the ferredoxin gene fdx1 and the reductase A2 gene redA2 of the electron transport system of the dioxin dioxygenase of Sphingomonas sp. strain RW1 and characterized the corresponding proteins. Fdx1 was found to be clustered with genes that appeared to encode two atypical decarboxylases and a glutathione-S transferase. A study was conducted to identify the open reading frames encoding the dioxin diogenase itself and derived functional expression of this enzyme system.

Published
1998

3. Characterization of 2,2',3-trihydroxybiphenyl dioxygenase, an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1

Author

Happe, Birgitta, Eltis, Lindsay D., Poth, Harald, Hedderich, Reiner, and Timmis, Kenneth N.

Subjects
Enzymes -- Analysis, Cloning -- Analysis, Dibenzofurans -- Analysis, Biological sciences
Abstract

The dbfB gene of the enzyme 2,2', 3-trihydroxybiphenyl dioxygenase, a monomeric protein, is cloned from a cosmid library of the Sphingomonas strain which degrades dibenzofuran and dibenzo-p-dioxin. Kinetic evaluation of the enzyme yields similar K(m) values for 2,3-dihydroxybiphenyl and 2,2',-3-trihydroxybiphenyl.

Published
1993

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