Search

Your search keyword '"Harpur, Christopher M."' showing total 24 results
Start Over Author "Harpur, Christopher M."
Sorry, I don't understand your search. ×
24 results on '"Harpur, Christopher M."'

2. Harnessing Endogenous Peptide Compounds as Potential Therapeutics for Severe Influenza.

Author

West, Alison C, Harpur, Christopher M, Page, Mélanie A Le, Lam, Maggie, Hodges, Christopher, Ely, Lauren K, Gearing, Andrew J, and Tate, Michelle D

Subjects
*HUMAN growth hormone, *CYCLIC peptides, *VIRUS diseases, *PEPTIDES, *ALVEOLAR macrophages
Abstract

Background Excessive pulmonary inflammation and damage are characteristic features of severe influenza virus infections. LAT8881 is a synthetic 16–amino acid cyclic peptide form of a naturally occurring C-terminal fragment of human growth hormone with therapeutic efficacy against influenza. Shorter linear peptides are typically easier to manufacture and formulate for delivery than larger cyclic peptides. A 6–amino acid linear peptide fragment of LAT8881, LAT9997, was investigated as a potential influenza therapy. Methods LAT9997 was evaluated for its potential to limit disease in a preclinical mouse model of severe influenza infection. Results Intranasal treatment of mice with either LAT8881 or LAT9997 from day 1 following influenza infection significantly improved survival outcomes. Initiating LAT9997 treatment at the onset of severe disease also significantly improved disease severity. Greater disease resistance in LAT9997-treated mice correlated with reduced lung immunopathology, damage markers, vascular leak, and epithelial cell death. Treatment reduced viral loads, cytokines, and neutrophil infiltration in the airways yet maintained protective alveolar macrophages in a dose-dependent manner. Sequential trimming of N- and C-terminal amino acids from LAT9997 revealed a structure-activity relationship. Conclusions These findings provide preclinical evidence that therapeutic LAT9997 treatment limits viral burden and characteristic features of severe influenza, including hyperinflammation and lung damage. Summary Excessive pulmonary inflammation and damage are characteristic features of severe influenza virus infections. LAT9997 is a linear peptide fragment derived from human growth hormone. This study provides preclinical evidence that therapeutic LAT9997 treatment limits viral burden, hyperinflammation, and lung damage. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

6. A high‐dimensional cytometry atlas of peripheral blood over the human life span

Author

Jalali, Sedigheh, primary, Harpur, Christopher M, additional, Piers, Adam T, additional, Auladell, Maria, additional, Perriman, Louis, additional, Li, Shuo, additional, An, Kim, additional, Anderson, Jeremy, additional, Berzins, Stuart P, additional, Licciardi, Paul V, additional, Ashhurst, Thomas M, additional, Konstantinov, Igor E, additional, and Pellicci, Daniel G, additional

Published
2022
Full Text
View/download PDF

7. Blockade of the protease ADAM17 ameliorates experimental pancreatitis

Author

Saad, Mohamed I., primary, Weng, Teresa, additional, Lundy, Joanne, additional, Gearing, Linden J., additional, West, Alison C., additional, Harpur, Christopher M., additional, Alanazi, Mohammad, additional, Hodges, Christopher, additional, Croagh, Daniel, additional, Kumar, Beena, additional, Sagi, Irit, additional, Rose-John, Stefan, additional, and Jenkins, Brendan J., additional

Published
2022
Full Text
View/download PDF

9. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Author

Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Abrignani, Sergio, additional, Addo, Richard, additional, Akdis, Mübeccel, additional, Andrä, Immanuel, additional, Andreata, Francesco, additional, Annunziato, Francesco, additional, Arranz, Eduardo, additional, Bacher, Petra, additional, Bari, Sudipto, additional, Barnaba, Vincenzo, additional, Barros‐Martins, Joana, additional, Baumjohann, Dirk, additional, Beccaria, Cristian G., additional, Bernardo, David, additional, Boardman, Dominic A., additional, Borger, Jessica, additional, Böttcher, Chotima, additional, Brockmann, Leonie, additional, Burns, Marie, additional, Busch, Dirk H., additional, Cameron, Garth, additional, Cammarata, Ilenia, additional, Cassotta, Antonino, additional, Chang, Yinshui, additional, Chirdo, Fernando Gabriel, additional, Christakou, Eleni, additional, Čičin‐Šain, Luka, additional, Cook, Laura, additional, Corbett, Alexandra J., additional, Cornelis, Rebecca, additional, Cosmi, Lorenzo, additional, Davey, Martin S., additional, De Biasi, Sara, additional, De Simone, Gabriele, additional, del Zotto, Genny, additional, Delacher, Michael, additional, Di Rosa, Francesca, additional, Di Santo, James, additional, Diefenbach, Andreas, additional, Dong, Jun, additional, Dörner, Thomas, additional, Dress, Regine J., additional, Dutertre, Charles‐Antoine, additional, Eckle, Sidonia B. G., additional, Eede, Pascale, additional, Evrard, Maximilien, additional, Falk, Christine S., additional, Feuerer, Markus, additional, Fillatreau, Simon, additional, Fiz‐Lopez, Aida, additional, Follo, Marie, additional, Foulds, Gemma A., additional, Fröbel, Julia, additional, Gagliani, Nicola, additional, Galletti, Giovanni, additional, Gangaev, Anastasia, additional, Garbi, Natalio, additional, Garrote, José Antonio, additional, Geginat, Jens, additional, Gherardin, Nicholas A., additional, Gibellini, Lara, additional, Ginhoux, Florent, additional, Godfrey, Dale I., additional, Gruarin, Paola, additional, Haftmann, Claudia, additional, Hansmann, Leo, additional, Harpur, Christopher M., additional, Hayday, Adrian C., additional, Heine, Guido, additional, Hernández, Daniela Carolina, additional, Herrmann, Martin, additional, Hoelsken, Oliver, additional, Huang, Qing, additional, Huber, Samuel, additional, Huber, Johanna E., additional, Huehn, Jochen, additional, Hundemer, Michael, additional, Hwang, William Y. K., additional, Iannacone, Matteo, additional, Ivison, Sabine M., additional, Jäck, Hans‐Martin, additional, Jani, Peter K., additional, Keller, Baerbel, additional, Kessler, Nina, additional, Ketelaars, Steven, additional, Knop, Laura, additional, Knopf, Jasmin, additional, Koay, Hui‐Fern, additional, Kobow, Katja, additional, Kriegsmann, Katharina, additional, Kristyanto, H., additional, Krueger, Andreas, additional, Kuehne, Jenny F., additional, Kunze‐Schumacher, Heike, additional, Kvistborg, Pia, additional, Kwok, Immanuel, additional, Latorre, Daniela, additional, Lenz, Daniel, additional, Levings, Megan K., additional, Lino, Andreia C., additional, Liotta, Francesco, additional, Long, Heather M., additional, Lugli, Enrico, additional, MacDonald, Katherine N., additional, Maggi, Laura, additional, Maini, Mala K., additional, Mair, Florian, additional, Manta, Calin, additional, Manz, Rudolf Armin, additional, Mashreghi, Mir‐Farzin, additional, Mazzoni, Alessio, additional, McCluskey, James, additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Monin, Leticia, additional, Moretta, Lorenzo, additional, Multhoff, Gabriele, additional, Muñoz, Luis Enrique, additional, Muñoz‐Ruiz, Miguel, additional, Muscate, Franziska, additional, Natalini, Ambra, additional, Neumann, Katrin, additional, Ng, Lai Guan, additional, Niedobitek, Antonia, additional, Niemz, Jana, additional, Almeida, Larissa Nogueira, additional, Notarbartolo, Samuele, additional, Ostendorf, Lennard, additional, Pallett, Laura J., additional, Patel, Amit A., additional, Percin, Gulce Itir, additional, Peruzzi, Giovanna, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Pracht, Katharina, additional, Prinz, Immo, additional, Pujol‐Autonell, Irma, additional, Pulvirenti, Nadia, additional, Quatrini, Linda, additional, Quinn, Kylie M., additional, Radbruch, Helena, additional, Rhys, Hefin, additional, Rodrigo, Maria B., additional, Romagnani, Chiara, additional, Saggau, Carina, additional, Sakaguchi, Shimon, additional, Sallusto, Federica, additional, Sanderink, Lieke, additional, Sandrock, Inga, additional, Schauer, Christine, additional, Scheffold, Alexander, additional, Scherer, Hans U., additional, Schiemann, Matthias, additional, Schildberg, Frank A., additional, Schober, Kilian, additional, Schoen, Janina, additional, Schuh, Wolfgang, additional, Schüler, Thomas, additional, Schulz, Axel R., additional, Schulz, Sebastian, additional, Schulze, Julia, additional, Simonetti, Sonia, additional, Singh, Jeeshan, additional, Sitnik, Katarzyna M., additional, Stark, Regina, additional, Starossom, Sarah, additional, Stehle, Christina, additional, Szelinski, Franziska, additional, Tan, Leonard, additional, Tarnok, Attila, additional, Tornack, Julia, additional, Tree, Timothy I. M., additional, van Beek, Jasper J. P., additional, van de Veen, Willem, additional, van Gisbergen, Klaas, additional, Vasco, Chiara, additional, Verheyden, Nikita A., additional, von Borstel, Anouk, additional, Ward‐Hartstonge, Kirsten A., additional, Warnatz, Klaus, additional, Waskow, Claudia, additional, Wiedemann, Annika, additional, Wilharm, Anneke, additional, Wing, James, additional, Wirz, Oliver, additional, Wittner, Jens, additional, Yang, Jennie H. M., additional, and Yang, Juhao, additional

Published
2021
Full Text
View/download PDF

10. Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses

Author

Anderson, Jeremy, primary, Thang, Cao Minh, additional, Thanh, Le Quang, additional, Dai, Vo Thi Trang, additional, Phan, Van Thanh, additional, Nhu, Bui Thi Hong, additional, Trang, Do Ngoc Xuan, additional, Trinh, Phan Thi Phuong, additional, Nguyen, Thuong Vu, additional, Toan, Nguyen Trong, additional, Harpur, Christopher M., additional, Mulholland, Kim, additional, Pellicci, Daniel G., additional, Do, Lien Anh Ha, additional, and Licciardi, Paul V., additional

Published
2021
Full Text
View/download PDF

12. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Author

Rolf M. Schwiete Foundation, Associazione Italiana per la Ricerca sul Cancro, German Research Foundation, National Institutes of Health (US), European Commission, Cossarizza, Andrea, Chang, Hyun‐Dong, Radbruch, Andreas, Abrignani, Sergio, Addo, Richard, Akdis, Mübeccel, Andrä, Immanuel, Andreata, Francesco, Annunziato, Francesco, Arranz, Eduardo, Bacher, Petra, Knop, Laura, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kriegsmann, Katharina, Kristyanto, H., Krueger, Andreas, Kuehne, Jenny F., Kunze-Schumacher, Heike, Maini, Mala K., Verheyden, Nikita A., Kvistborg, Pia, Kwok, Immanuel, Latorre, Daniela, Mair, Florian, Bari, Sudipto, Manta, Calin, Armin Manz, Rudolf, Mashreghi, Mir-Farzin, Mazzoni, Alessio, McCluskey, James, Borstel, Anouk von, Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Monin, Leticia, Barnaba, Vincenzo, Moretta, Lorenzo, Multhoff, Gabriele, Muñoz, Luis Enrique, Muñoz-Ruiz, Miguel, Ward-Hartstonge, Kirsten A., Muscate, Franziska, Natalini, Ambra, Neumann, Katrin, Guan N., Lai, Niedobitek, Antonia, Niemz, Jana, Barros-Martins, Joana, Nogueira Almeida, Larissa, Notarbartolo, Samuele, Ostendorf, Lennard, Warnatz, Klaus, Pallett, Laura J., Patel, Amit A., Itir Percin, Gulce, Peruzzi, Giovanna, Pinti, Marcello, Pockley, A. Graham, Pracht, Katharina, Baumjohann, Dirk, Prinz, Immo, Pujol-Autonell, Irma, Waskow, Claudia, Pulvirenti, Nadia, Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Rhys, Hefin, Rodrigo, Maria B., Romagnani, Chiara, Saggau, Carina, Beccaria, Cristian G., Sakaguchi, Shimon, Wiedemann, Annika, Sallusto, Federica, Sanderink, Lieke, Sandrock, Inga, Schauer, Christine, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schober, Kilian, Bernardo, David, Wilharm, Anneke, Schoen, Janina, Schuh, Wolfgang, Schüler, Thomas, Schulz, Axel R., Schulz, Sebastian, Schulze, Julia, Simonetti, Sonia, Singh, Jeeshan, Sitnik, Katarzyna M., Stark, Regina, Wing, James, Boardman, Dominic A., Starossom, Sarah, Stehle, Christina, Szelinski, Franziska, Tan, Leonard, Tarnok, Attila, Tornack, Julia, Tree, Timothy I. M., Van Beek, Jasper J. P., Veen, Willem van de, Wirz, Oliver, Gisbergen, Klaas van, Borger, Jessica, Vasco, Chiara, Böttcher, Chotima, Lenz, Daniel, Wittner, Jens, Yang, Jennie H. M., Yang, Juhao, Brockmann, Leonie, Burns, Marie, Busch, Dirk H., Cameron, Garth, Cammarata, Ilenia, Cassotta, Antonino, Chang, Yinshui, Levings, Megan K., Chirdo, Fernando G., Christakou, Eleni, Cicin-Sain, Luka, Cook, Laura, Corbett, Alexandra J., Cornelis, Rebecca, Cosmi, Lorenzo, Davey, Martin S., Biasi, Sara De, Simone, Gabriele De, Lino, Andreia C., Zotto, Genny del, Delacher, Michael, Rosa, Francesca Di, Santo, James Di, Diefenbach, Andreas, Dong, Jun, Dörner, Thomas, Dress, Regine J., Dutertre, Charles-Antoine, Eckle, Sidonia B. G., Liotta, Francesco, Eede, Pascale, Evrard, Maximilien, Falk, Christine S., Feuerer, Markus, Fillatreau, Simon, Fiz-López, Aida, Follo, Marie, Foulds, Gemma A., Fröbel, Julia, Gagliani, Nicola, Long, Heather M., Galletti, Giovanni, Gangaev, Anastasia, Garbi, Natalio, Garrote, José Antonio, Geginat, Jens, Gherardin, Nicholas A., Gibellini, Lara, Ginhoux, Florent, Godfrey, Dale I., Gruarin, Paola, Lugli, Enrico, Haftmann, Claudia, Hansmann, Leo, Harpur, Christopher M., Hayday, Adrian C., Heine, Guido, Hernández, Daniela Carolina, Herrmann, Martin, Hoelsken, Oliver, Huang, Qing, Huber, Samuel, MacDonald, Katherine N., Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hwang, William Y. K., Iannacone, Matteo, Ivison, Sabine M., Jäck, Hans-Martin, Jani, Peter K., Keller, Baerbel, Kessler, Nina, Maggi, Laura, Ketelaars, Steven, Rolf M. Schwiete Foundation, Associazione Italiana per la Ricerca sul Cancro, German Research Foundation, National Institutes of Health (US), European Commission, Cossarizza, Andrea, Chang, Hyun‐Dong, Radbruch, Andreas, Abrignani, Sergio, Addo, Richard, Akdis, Mübeccel, Andrä, Immanuel, Andreata, Francesco, Annunziato, Francesco, Arranz, Eduardo, Bacher, Petra, Knop, Laura, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kriegsmann, Katharina, Kristyanto, H., Krueger, Andreas, Kuehne, Jenny F., Kunze-Schumacher, Heike, Maini, Mala K., Verheyden, Nikita A., Kvistborg, Pia, Kwok, Immanuel, Latorre, Daniela, Mair, Florian, Bari, Sudipto, Manta, Calin, Armin Manz, Rudolf, Mashreghi, Mir-Farzin, Mazzoni, Alessio, McCluskey, James, Borstel, Anouk von, Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Monin, Leticia, Barnaba, Vincenzo, Moretta, Lorenzo, Multhoff, Gabriele, Muñoz, Luis Enrique, Muñoz-Ruiz, Miguel, Ward-Hartstonge, Kirsten A., Muscate, Franziska, Natalini, Ambra, Neumann, Katrin, Guan N., Lai, Niedobitek, Antonia, Niemz, Jana, Barros-Martins, Joana, Nogueira Almeida, Larissa, Notarbartolo, Samuele, Ostendorf, Lennard, Warnatz, Klaus, Pallett, Laura J., Patel, Amit A., Itir Percin, Gulce, Peruzzi, Giovanna, Pinti, Marcello, Pockley, A. Graham, Pracht, Katharina, Baumjohann, Dirk, Prinz, Immo, Pujol-Autonell, Irma, Waskow, Claudia, Pulvirenti, Nadia, Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Rhys, Hefin, Rodrigo, Maria B., Romagnani, Chiara, Saggau, Carina, Beccaria, Cristian G., Sakaguchi, Shimon, Wiedemann, Annika, Sallusto, Federica, Sanderink, Lieke, Sandrock, Inga, Schauer, Christine, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schober, Kilian, Bernardo, David, Wilharm, Anneke, Schoen, Janina, Schuh, Wolfgang, Schüler, Thomas, Schulz, Axel R., Schulz, Sebastian, Schulze, Julia, Simonetti, Sonia, Singh, Jeeshan, Sitnik, Katarzyna M., Stark, Regina, Wing, James, Boardman, Dominic A., Starossom, Sarah, Stehle, Christina, Szelinski, Franziska, Tan, Leonard, Tarnok, Attila, Tornack, Julia, Tree, Timothy I. M., Van Beek, Jasper J. P., Veen, Willem van de, Wirz, Oliver, Gisbergen, Klaas van, Borger, Jessica, Vasco, Chiara, Böttcher, Chotima, Lenz, Daniel, Wittner, Jens, Yang, Jennie H. M., Yang, Juhao, Brockmann, Leonie, Burns, Marie, Busch, Dirk H., Cameron, Garth, Cammarata, Ilenia, Cassotta, Antonino, Chang, Yinshui, Levings, Megan K., Chirdo, Fernando G., Christakou, Eleni, Cicin-Sain, Luka, Cook, Laura, Corbett, Alexandra J., Cornelis, Rebecca, Cosmi, Lorenzo, Davey, Martin S., Biasi, Sara De, Simone, Gabriele De, Lino, Andreia C., Zotto, Genny del, Delacher, Michael, Rosa, Francesca Di, Santo, James Di, Diefenbach, Andreas, Dong, Jun, Dörner, Thomas, Dress, Regine J., Dutertre, Charles-Antoine, Eckle, Sidonia B. G., Liotta, Francesco, Eede, Pascale, Evrard, Maximilien, Falk, Christine S., Feuerer, Markus, Fillatreau, Simon, Fiz-López, Aida, Follo, Marie, Foulds, Gemma A., Fröbel, Julia, Gagliani, Nicola, Long, Heather M., Galletti, Giovanni, Gangaev, Anastasia, Garbi, Natalio, Garrote, José Antonio, Geginat, Jens, Gherardin, Nicholas A., Gibellini, Lara, Ginhoux, Florent, Godfrey, Dale I., Gruarin, Paola, Lugli, Enrico, Haftmann, Claudia, Hansmann, Leo, Harpur, Christopher M., Hayday, Adrian C., Heine, Guido, Hernández, Daniela Carolina, Herrmann, Martin, Hoelsken, Oliver, Huang, Qing, Huber, Samuel, MacDonald, Katherine N., Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hwang, William Y. K., Iannacone, Matteo, Ivison, Sabine M., Jäck, Hans-Martin, Jani, Peter K., Keller, Baerbel, Kessler, Nina, Maggi, Laura, and Ketelaars, Steven

Abstract

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Published
2021

13. Killer cell immunoglobulin-like receptor 3DL1-mediated recognition of human leukocyte antigen

Author

Vivian, Julian P., Duncan, Renee C., Berry, Richard, O'Connor, Geraldine M., Reid, Hugh H., Beddoe, Travis, Gras, Stephanie, Saunders, Philippa M., Olshina, Maya A., Widjaja, Jacqueline M.L., Harpur, Christopher M., Lin, Jie, Maloveste, Sebastien M., Price, David A., Lafont, Bernard A.P., McVicar, Daniel W., Clements, Craig S., Brooks, Andrew G., and Rossjohn, Jamie

Subjects
Immunoglobulins -- Genetic aspects, Killer cells -- Genetic aspects, Leukocytes -- Health aspects -- Genetic aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Members of the killer cell immunoglobulin-like receptor (KIR) family, a large group of polymorphic receptors expressed on natural killer (NK) cells, recognize particular peptide-laden human leukocyte antigen (pHLA) class I molecules and have a pivotal role in innate immune responses (1). Allelic variation and extensive polymorphism within the three-domain KIR family (KIR3D, domains D0-D1-D2) affects pHLA binding specificity and is linked to the control of viral replication and the treatment outcome of certain haematological malignancies (1-3). Here we describe the structure of a human KIR3DL1 receptor bound to HLA-B*5701 complexed with a self-peptide. KIR3DL1 clamped around the carboxy-terminal end of the HLA-B*5701 antigen-binding cleft, resulting in two discontinuous footprints on the pHLA. First, the D0 domain, a distinguishing feature of the KIR3D family, extended towards β2-microglobulin and abutted a region of the HLA molecule with limited polymorphism, thereby acting as an 'innate HLA sensor' domain. Second, whereas the D2-HLA-B*5701 interface exhibited a high degree of complementarity, the D1-pHLA-B*5701 contacts were suboptimal and accommodated a degree of sequence variation both within the peptide and the polymorphic region of the HLA molecule. Although the two-domain KIR (KIR2D) and KIR3DL1 docked similarly onto HLA-C (4,5) and HLA-B respectively, the corresponding Dl-mediated interactions differed markedly, thereby providing insight into the specificity of KIR3DL1 for discrete HLA-A and HLA-B allotypes. Collectively, in association with extensive mutagenesis studies at the KIR3DL1-pHLA-B*5701 interface, we provide a framework for understanding the intricate interplay between peptide variability, KIR3D and HLA polymorphism in determining the specificity requirements of this essential innate interaction that is conserved across primate species., HLA-B57 carriage has been associated with delayed progression to AIDS in HIV-infected individuals, with a strong genetic association between the KIR3DL1-HLA-B57 interaction, reduced viral loads and delayed HIV disease progression [...]

Published
2011
Full Text
View/download PDF

15. Natural killer cell receptors regulate responses of HLA-E–restricted T cells

Author

Sullivan, Lucy C., primary, Nguyen, Thi H.O., additional, Harpur, Christopher M., additional, Stankovic, Sanda, additional, Kanagarajah, Abbie R., additional, Koutsakos, Marios, additional, Saunders, Philippa M., additional, Cai, Zhangying, additional, Gray, James A., additional, Widjaja, Jacqueline M.L., additional, Lin, Jie, additional, Pietra, Gabriella, additional, Mingari, Maria Cristina, additional, Moretta, Lorenzo, additional, Samir, Jerome, additional, Luciani, Fabio, additional, Westall, Glen P., additional, Malmberg, Karl J., additional, Kedzierska, Katherine, additional, and Brooks, Andrew G., additional

Published
2021
Full Text
View/download PDF

16. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author

Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, Zychlinsky, Arturo, Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Acs, Andreas, Adam, Dieter, Adam-Klages, Sabine, Agace, William W., Aghaeepour, Nima, Akdis, Muebeccel, Allez, Matthieu, Almeida, Larissa Nogueira, Alvisi, Giorgia, Anderson, Graham, Andrae, Immanuel, Annunziato, Francesco, Anselmo, Achille, Bacher, Petra, Baldari, Cosima T., Bari, Sudipto, Barnaba, Vincenzo, Barros-Martins, Joana, Battistini, Luca, Bauer, Wolfgang, Baumgart, Sabine, Baumgarth, Nicole, Baumjohann, Dirk, Baying, Bianka, Bebawy, Mary, Becher, Burkhard, Beisker, Wolfgang, Benes, Vladimir, Beyaert, Rudi, Blanco, Alfonso, Boardman, Dominic A., Bogdan, Christian, Borger, Jessica G., Borsellino, Giovanna, Boulais, Philip E., Bradford, Jolene A., Brenner, Dirk, Brinkman, Ryan R., Brooks, Anna E. S., Busch, Dirk H., Buescher, Martin, Bushnell, Timothy P., Calzetti, Federica, Cameron, Garth, Cammarata, Ilenia, Cao, Xuetao, Cardell, Susanna L., Casola, Stefano, Cassatella, Marco A., Cavani, Andrea, Celada, Antonio, Chatenoud, Lucienne, Chattopadhyay, Pratip K., Chow, Sue, Christakou, Eleni, Cicin-Sain, Luka, Clerici, Mario, Colombo, Federico S., Cook, Laura, Cooke, Anne, Cooper, Andrea M., Corbett, Alexandra J., Cosma, Antonio, Cosmi, Lorenzo, Coulie, Pierre G., Cumano, Ana, Cvetkovic, Ljiljana, Dang, Van Duc, Dang-Heine, Chantip, Davey, Martin S., Davies, Derek, De Biasi, Sara, Del Zotto, Genny, Dela Cruz, Gelo Victoriano, Delacher, Michael, Della Bella, Silvia, Dellabona, Paolo, Deniz, Guennur, Dessing, Mark, Di Santo, James P., Diefenbach, Andreas, Dieli, Francesco, Dolf, Andreas, Doerner, Thomas, Dress, Regine J., Dudziak, Diana, Dustin, Michael, Dutertre, Charles-Antoine, Ebner, Friederike, Eckle, Sidonia B. G., Edinger, Matthias, Eede, Pascale, Ehrhardt, Goetz R. A., Eich, Marcus, Engel, Pablo, Engelhardt, Britta, Erdei, Anna, Esser, Charlotte, Everts, Bart, Evrard, Maximilien, Falk, Christine S., Fehniger, Todd A., Felipo-Benavent, Mar, Ferry, Helen, Feuerer, Markus, Filby, Andrew, Filkor, Kata, Fillatreau, Simon, Follo, Marie, Foerster, Irmgard, Foster, John, Foulds, Gemma A., Frehse, Britta, Frenette, Paul S., Frischbutter, Stefan, Fritzsche, Wolfgang, Galbraith, David W., Gangaev, Anastasia, Garbi, Natalio, Gaudilliere, Brice, Gazzinelli, Ricardo T., Geginat, Jens, Gerner, Wilhelm, Gherardin, Nicholas A., Ghoreschi, Kamran, Gibellini, Lara, Ginhoux, Florent, Goda, Keisuke, Godfrey, Dale I., Goettlinger, Christoph, Gonzalez-Navajas, Jose M., Goodyear, Carl S., Gori, Andrea, Grogan, Jane L., Grummitt, Daryl, Gruetzkau, Andreas, Haftmann, Claudia, Hahn, Jonas, Hammad, Hamida, Haemmerling, Guenter, Hansmann, Leo, Hansson, Goran, Harpur, Christopher M., Hartmann, Susanne, Hauser, Andrea, Hauser, Anja E., Haviland, David L., Hedley, David, Hernandez, Daniela C., Herrera, Guadalupe, Herrmann, Martin, Hess, Christoph, Hoefer, Thomas, Hoffmann, Petra, Hogquist, Kristin, Holland, Tristan, Hollt, Thomas, Holmdahl, Rikard, Hombrink, Pleun, Houston, Jessica P., Hoyer, Bimba F., Huang, Bo, Huang, Fang-Ping, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hunter, Christopher A., Hwang, William Y. K., Iannone, Anna, Ingelfinger, Florian, Ivison, Sabine M., Jaeck, Hans-Martin, Jani, Peter K., Javega, Beatriz, Jonjic, Stipan, Kaiser, Toralf, Kalina, Tomas, Kamradt, Thomas, Kaufmann, Stefan H. E., Keller, Baerbel, Ketelaars, Steven L. C., Khalilnezhad, Ahad, Khan, Srijit, Kisielow, Jan, Klenerman, Paul, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kolls, Jay K., Kong, Wan Ting, Kopf, Manfred, Korn, Thomas, Kriegsmann, Katharina, Kristyanto, Hendy, Kroneis, Thomas, Krueger, Andreas, Kuehne, Jenny, Kukat, Christian, Kunkel, Desiree, Kunze-Schumacher, Heike, Kurosaki, Tomohiro, Kurts, Christian, Kvistborg, Pia, Kwok, Immanuel, Landry, Jonathan, Lantz, Olivier, Lanuti, Paola, LaRosa, Francesca, Lehuen, Agnes, LeibundGut-Landmann, Salome, Leipold, Michael D., Leung, Leslie Y. T., Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Litwin, Virginia, Liu, Yanling, Ljunggren, Hans-Gustaf, Lohoff, Michael, Lombardi, Giovanna, Lopez, Lilly, Lopez-Botet, Miguel, Lovett-Racke, Amy E., Lubberts, Erik, Luche, Herve, Ludewig, Burkhard, Lugli, Enrico, Lunemann, Sebastian, Maecker, Holden T., Maggi, Laura, Maguire, Orla, Mair, Florian, Mair, Kerstin H., Mantovani, Alberto, Manz, Rudolf A., Marshall, Aaron J., Martinez-Romero, Alicia, Martrus, Gloria, Marventano, Ivana, Maslinski, Wlodzimierz, Matarese, Giuseppe, Mattioli, Anna Vittoria, Maueroder, Christian, Mazzoni, Alessio, McCluskey, James, McGrath, Mairi, McGuire, Helen M., McInnes, Iain B., Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Miller, Stephen D., Mills, Kingston H. G., Minderman, Hans, Mjosberg, Jenny, Moore, Jonni, Moran, Barry, Moretta, Lorenzo, Mosmann, Tim R., Mueller, Susann, Multhoff, Gabriele, Munoz, Luis Enrique, Munz, Christian, Nakayama, Toshinori, Nasi, Milena, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Nourshargh, Sussan, Nunez, Gabriel, O'Connor, Jose-Enrique, Ochel, Aaron, Oja, Anna, Ordonez, Diana, Orfao, Alberto, Orlowski-Oliver, Eva, Ouyang, Wenjun, Oxenius, Annette, Palankar, Raghavendra, Panse, Isabel, Pattanapanyasat, Kovit, Paulsen, Malte, Pavlinic, Dinko, Penter, Livius, Peterson, Paert, Peth, Christian, Petriz, Jordi, Piancone, Federica, Pickl, Winfried F., Piconese, Silvia, Pinti, Marcello, Pockley, A. Graham, Podolska, Malgorzata Justyna, Poon, Zhiyong, Pracht, Katharina, Prinz, Immo, Pucillo, Carlo E. M., Quataert, Sally A., Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Radstake, Tim R. D. J., Rahmig, Susann, Rahn, Hans-Peter, Rajwa, Bartek, Ravichandran, Gevitha, Raz, Yotam, Rebhahn, Jonathan A., Recktenwald, Diether, Reimer, Dorothea, Reis e Sousa, Caetano, Remmerswaal, Ester B. M., Richter, Lisa, Rico, Laura G., Riddell, Andy, Rieger, Aja M., Robinson, J. Paul, Romagnani, Chiara, Rubartelli, Anna, Ruland, Juergen, Saalmueller, Armin, Saeys, Yvan, Saito, Takashi, Sakaguchi, Shimon, Sala-de-Oyanguren, Francisco, Samstag, Yvonne, Sanderson, Sharon, Sandrock, Inga, Santoni, Angela, Sanz, Ramon Bellmas, Saresella, Marina, Sautes-Fridman, Catherine, Sawitzki, Birgit, Schadt, Linda, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schimisky, Esther, Schlitzer, Andreas, Schlosser, Josephine, Schmid, Stephan, Schmitt, Steffen, Schober, Kilian, Schraivogel, Daniel, Schuh, Wolfgang, Schueler, Thomas, Schulte, Reiner, Schulz, Axel Ronald, Schulz, Sebastian R., Scotta, Cristiano, Scott-Algara, Daniel, Sester, David P., Shankey, T. Vincent, Silva-Santos, Bruno, Simon, Anna Katharina, Sitnik, Katarzyna M., Sozzani, Silvano, Speiser, Daniel E., Spidlen, Josef, Stahlberg, Anders, Stall, Alan M., Stanley, Natalie, Stark, Regina, Stehle, Christina, Steinmetz, Tobit, Stockinger, Hannes, Takahama, Yousuke, Takeda, Kiyoshi, Tan, Leonard, Tarnok, Attila, Tiegs, Gisa, Toldi, Gergely, Tornack, Julia, Traggiai, Elisabetta, Trebak, Mohamed, Tree, Timothy I. M., Trotter, Joe, Trowsdale, John, Tsoumakidou, Maria, Ulrich, Henning, Urbanczyk, Sophia, van de Veen, Willem, van den Broek, Maries, van der Pol, Edwin, Van Gassen, Sofie, Van Isterdael, Gert, van Lier, Rene A. W., Veldhoen, Marc, Vento-Asturias, Salvador, Vieira, Paulo, Voehringer, David, Volk, Hans-Dieter, von Borstel, Anouk, von Volkmann, Konrad, Waisman, Ari, Walker, Rachael V., Wallace, Paul K., Wang, Sa A., Wang, Xin M., Ward, Michael D., Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Warnes, Gary, Warth, Sarah, Waskow, Claudia, Watson, James V., Watzl, Carsten, Wegener, Leonie, Weisenburger, Thomas, Wiedemann, Annika, Wienands, Juergen, Wilharm, Anneke, Wilkinson, Robert John, Willimsky, Gerald, Wing, James B., Winkelmann, Rieke, Winkler, Thomas H., Wirz, Oliver F., Wong, Alicia, Wurst, Peter, Yang, Jennie H. M., Yang, Juhao, Yazdanbakhsh, Maria, Yu, Liping, Yue, Alice, Zhang, Hanlin, Zhao, Yi, Ziegler, Susanne Maria, Zielinski, Christina, Zimmermann, Jakob, and Zychlinsky, Arturo

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Published
2019

17. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Author

Cossarizza, Andrea, primary, Chang, Hyun‐Dong, additional, Radbruch, Andreas, additional, Acs, Andreas, additional, Adam, Dieter, additional, Adam‐Klages, Sabine, additional, Agace, William W., additional, Aghaeepour, Nima, additional, Akdis, Mübeccel, additional, Allez, Matthieu, additional, Almeida, Larissa Nogueira, additional, Alvisi, Giorgia, additional, Anderson, Graham, additional, Andrä, Immanuel, additional, Annunziato, Francesco, additional, Anselmo, Achille, additional, Bacher, Petra, additional, Baldari, Cosima T., additional, Bari, Sudipto, additional, Barnaba, Vincenzo, additional, Barros‐Martins, Joana, additional, Battistini, Luca, additional, Bauer, Wolfgang, additional, Baumgart, Sabine, additional, Baumgarth, Nicole, additional, Baumjohann, Dirk, additional, Baying, Bianka, additional, Bebawy, Mary, additional, Becher, Burkhard, additional, Beisker, Wolfgang, additional, Benes, Vladimir, additional, Beyaert, Rudi, additional, Blanco, Alfonso, additional, Boardman, Dominic A., additional, Bogdan, Christian, additional, Borger, Jessica G., additional, Borsellino, Giovanna, additional, Boulais, Philip E., additional, Bradford, Jolene A., additional, Brenner, Dirk, additional, Brinkman, Ryan R., additional, Brooks, Anna E. S., additional, Busch, Dirk H., additional, Büscher, Martin, additional, Bushnell, Timothy P., additional, Calzetti, Federica, additional, Cameron, Garth, additional, Cammarata, Ilenia, additional, Cao, Xuetao, additional, Cardell, Susanna L., additional, Casola, Stefano, additional, Cassatella, Marco A., additional, Cavani, Andrea, additional, Celada, Antonio, additional, Chatenoud, Lucienne, additional, Chattopadhyay, Pratip K., additional, Chow, Sue, additional, Christakou, Eleni, additional, Čičin‐Šain, Luka, additional, Clerici, Mario, additional, Colombo, Federico S., additional, Cook, Laura, additional, Cooke, Anne, additional, Cooper, Andrea M., additional, Corbett, Alexandra J., additional, Cosma, Antonio, additional, Cosmi, Lorenzo, additional, Coulie, Pierre G., additional, Cumano, Ana, additional, Cvetkovic, Ljiljana, additional, Dang, Van Duc, additional, Dang‐Heine, Chantip, additional, Davey, Martin S., additional, Davies, Derek, additional, De Biasi, Sara, additional, Del Zotto, Genny, additional, Dela Cruz, Gelo Victoriano, additional, Delacher, Michael, additional, Della Bella, Silvia, additional, Dellabona, Paolo, additional, Deniz, Günnur, additional, Dessing, Mark, additional, Di Santo, James P., additional, Diefenbach, Andreas, additional, Dieli, Francesco, additional, Dolf, Andreas, additional, Dörner, Thomas, additional, Dress, Regine J., additional, Dudziak, Diana, additional, Dustin, Michael, additional, Dutertre, Charles‐Antoine, additional, Ebner, Friederike, additional, Eckle, Sidonia B. G., additional, Edinger, Matthias, additional, Eede, Pascale, additional, Ehrhardt, Götz R.A., additional, Eich, Marcus, additional, Engel, Pablo, additional, Engelhardt, Britta, additional, Erdei, Anna, additional, Esser, Charlotte, additional, Everts, Bart, additional, Evrard, Maximilien, additional, Falk, Christine S., additional, Fehniger, Todd A., additional, Felipo‐Benavent, Mar, additional, Ferry, Helen, additional, Feuerer, Markus, additional, Filby, Andrew, additional, Filkor, Kata, additional, Fillatreau, Simon, additional, Follo, Marie, additional, Förster, Irmgard, additional, Foster, John, additional, Foulds, Gemma A., additional, Frehse, Britta, additional, Frenette, Paul S., additional, Frischbutter, Stefan, additional, Fritzsche, Wolfgang, additional, Galbraith, David W., additional, Gangaev, Anastasia, additional, Garbi, Natalio, additional, Gaudilliere, Brice, additional, Gazzinelli, Ricardo T., additional, Geginat, Jens, additional, Gerner, Wilhelm, additional, Gherardin, Nicholas A., additional, Ghoreschi, Kamran, additional, Gibellini, Lara, additional, Ginhoux, Florent, additional, Goda, Keisuke, additional, Godfrey, Dale I., additional, Goettlinger, Christoph, additional, González‐Navajas, Jose M., additional, Goodyear, Carl S., additional, Gori, Andrea, additional, Grogan, Jane L., additional, Grummitt, Daryl, additional, Grützkau, Andreas, additional, Haftmann, Claudia, additional, Hahn, Jonas, additional, Hammad, Hamida, additional, Hämmerling, Günter, additional, Hansmann, Leo, additional, Hansson, Goran, additional, Harpur, Christopher M., additional, Hartmann, Susanne, additional, Hauser, Andrea, additional, Hauser, Anja E., additional, Haviland, David L., additional, Hedley, David, additional, Hernández, Daniela C., additional, Herrera, Guadalupe, additional, Herrmann, Martin, additional, Hess, Christoph, additional, Höfer, Thomas, additional, Hoffmann, Petra, additional, Hogquist, Kristin, additional, Holland, Tristan, additional, Höllt, Thomas, additional, Holmdahl, Rikard, additional, Hombrink, Pleun, additional, Houston, Jessica P., additional, Hoyer, Bimba F., additional, Huang, Bo, additional, Huang, Fang‐Ping, additional, Huber, Johanna E., additional, Huehn, Jochen, additional, Hundemer, Michael, additional, Hunter, Christopher A., additional, Hwang, William Y. K., additional, Iannone, Anna, additional, Ingelfinger, Florian, additional, Ivison, Sabine M, additional, Jäck, Hans‐Martin, additional, Jani, Peter K., additional, Jávega, Beatriz, additional, Jonjic, Stipan, additional, Kaiser, Toralf, additional, Kalina, Tomas, additional, Kamradt, Thomas, additional, Kaufmann, Stefan H. E., additional, Keller, Baerbel, additional, Ketelaars, Steven L. C., additional, Khalilnezhad, Ahad, additional, Khan, Srijit, additional, Kisielow, Jan, additional, Klenerman, Paul, additional, Knopf, Jasmin, additional, Koay, Hui‐Fern, additional, Kobow, Katja, additional, Kolls, Jay K., additional, Kong, Wan Ting, additional, Kopf, Manfred, additional, Korn, Thomas, additional, Kriegsmann, Katharina, additional, Kristyanto, Hendy, additional, Kroneis, Thomas, additional, Krueger, Andreas, additional, Kühne, Jenny, additional, Kukat, Christian, additional, Kunkel, Désirée, additional, Kunze‐Schumacher, Heike, additional, Kurosaki, Tomohiro, additional, Kurts, Christian, additional, Kvistborg, Pia, additional, Kwok, Immanuel, additional, Landry, Jonathan, additional, Lantz, Olivier, additional, Lanuti, Paola, additional, LaRosa, Francesca, additional, Lehuen, Agnès, additional, LeibundGut‐Landmann, Salomé, additional, Leipold, Michael D., additional, Leung, Leslie Y.T., additional, Levings, Megan K., additional, Lino, Andreia C., additional, Liotta, Francesco, additional, Litwin, Virginia, additional, Liu, Yanling, additional, Ljunggren, Hans‐Gustaf, additional, Lohoff, Michael, additional, Lombardi, Giovanna, additional, Lopez, Lilly, additional, López‐Botet, Miguel, additional, Lovett‐Racke, Amy E., additional, Lubberts, Erik, additional, Luche, Herve, additional, Ludewig, Burkhard, additional, Lugli, Enrico, additional, Lunemann, Sebastian, additional, Maecker, Holden T., additional, Maggi, Laura, additional, Maguire, Orla, additional, Mair, Florian, additional, Mair, Kerstin H., additional, Mantovani, Alberto, additional, Manz, Rudolf A., additional, Marshall, Aaron J., additional, Martínez‐Romero, Alicia, additional, Martrus, Glòria, additional, Marventano, Ivana, additional, Maslinski, Wlodzimierz, additional, Matarese, Giuseppe, additional, Mattioli, Anna Vittoria, additional, Maueröder, Christian, additional, Mazzoni, Alessio, additional, McCluskey, James, additional, McGrath, Mairi, additional, McGuire, Helen M., additional, McInnes, Iain B., additional, Mei, Henrik E., additional, Melchers, Fritz, additional, Melzer, Susanne, additional, Mielenz, Dirk, additional, Miller, Stephen D., additional, Mills, Kingston H.G., additional, Minderman, Hans, additional, Mjösberg, Jenny, additional, Moore, Jonni, additional, Moran, Barry, additional, Moretta, Lorenzo, additional, Mosmann, Tim R., additional, Müller, Susann, additional, Multhoff, Gabriele, additional, Muñoz, Luis Enrique, additional, Münz, Christian, additional, Nakayama, Toshinori, additional, Nasi, Milena, additional, Neumann, Katrin, additional, Ng, Lai Guan, additional, Niedobitek, Antonia, additional, Nourshargh, Sussan, additional, Núñez, Gabriel, additional, O'Connor, José‐Enrique, additional, Ochel, Aaron, additional, Oja, Anna, additional, Ordonez, Diana, additional, Orfao, Alberto, additional, Orlowski‐Oliver, Eva, additional, Ouyang, Wenjun, additional, Oxenius, Annette, additional, Palankar, Raghavendra, additional, Panse, Isabel, additional, Pattanapanyasat, Kovit, additional, Paulsen, Malte, additional, Pavlinic, Dinko, additional, Penter, Livius, additional, Peterson, Pärt, additional, Peth, Christian, additional, Petriz, Jordi, additional, Piancone, Federica, additional, Pickl, Winfried F., additional, Piconese, Silvia, additional, Pinti, Marcello, additional, Pockley, A. Graham, additional, Podolska, Malgorzata Justyna, additional, Poon, Zhiyong, additional, Pracht, Katharina, additional, Prinz, Immo, additional, Pucillo, Carlo E. M., additional, Quataert, Sally A., additional, Quatrini, Linda, additional, Quinn, Kylie M., additional, Radbruch, Helena, additional, Radstake, Tim R. D. J., additional, Rahmig, Susann, additional, Rahn, Hans‐Peter, additional, Rajwa, Bartek, additional, Ravichandran, Gevitha, additional, Raz, Yotam, additional, Rebhahn, Jonathan A., additional, Recktenwald, Diether, additional, Reimer, Dorothea, additional, Reis e Sousa, Caetano, additional, Remmerswaal, Ester B.M., additional, Richter, Lisa, additional, Rico, Laura G., additional, Riddell, Andy, additional, Rieger, Aja M., additional, Robinson, J. Paul, additional, Romagnani, Chiara, additional, Rubartelli, Anna, additional, Ruland, Jürgen, additional, Saalmüller, Armin, additional, Saeys, Yvan, additional, Saito, Takashi, additional, Sakaguchi, Shimon, additional, Sala‐de‐Oyanguren, Francisco, additional, Samstag, Yvonne, additional, Sanderson, Sharon, additional, Sandrock, Inga, additional, Santoni, Angela, additional, Sanz, Ramon Bellmàs, additional, Saresella, Marina, additional, Sautes‐Fridman, Catherine, additional, Sawitzki, Birgit, additional, Schadt, Linda, additional, Scheffold, Alexander, additional, Scherer, Hans U., additional, Schiemann, Matthias, additional, Schildberg, Frank A., additional, Schimisky, Esther, additional, Schlitzer, Andreas, additional, Schlosser, Josephine, additional, Schmid, Stephan, additional, Schmitt, Steffen, additional, Schober, Kilian, additional, Schraivogel, Daniel, additional, Schuh, Wolfgang, additional, Schüler, Thomas, additional, Schulte, Reiner, additional, Schulz, Axel Ronald, additional, Schulz, Sebastian R., additional, Scottá, Cristiano, additional, Scott‐Algara, Daniel, additional, Sester, David P., additional, Shankey, T. Vincent, additional, Silva‐Santos, Bruno, additional, Simon, Anna Katharina, additional, Sitnik, Katarzyna M., additional, Sozzani, Silvano, additional, Speiser, Daniel E., additional, Spidlen, Josef, additional, Stahlberg, Anders, additional, Stall, Alan M., additional, Stanley, Natalie, additional, Stark, Regina, additional, Stehle, Christina, additional, Steinmetz, Tobit, additional, Stockinger, Hannes, additional, Takahama, Yousuke, additional, Takeda, Kiyoshi, additional, Tan, Leonard, additional, Tárnok, Attila, additional, Tiegs, Gisa, additional, Toldi, Gergely, additional, Tornack, Julia, additional, Traggiai, Elisabetta, additional, Trebak, Mohamed, additional, Tree, Timothy I.M., additional, Trotter, Joe, additional, Trowsdale, John, additional, Tsoumakidou, Maria, additional, Ulrich, Henning, additional, Urbanczyk, Sophia, additional, van de Veen, Willem, additional, van den Broek, Maries, additional, van der Pol, Edwin, additional, Van Gassen, Sofie, additional, Van Isterdael, Gert, additional, van Lier, René A.W., additional, Veldhoen, Marc, additional, Vento‐Asturias, Salvador, additional, Vieira, Paulo, additional, Voehringer, David, additional, Volk, Hans‐Dieter, additional, von Borstel, Anouk, additional, von Volkmann, Konrad, additional, Waisman, Ari, additional, Walker, Rachael V., additional, Wallace, Paul K., additional, Wang, Sa A., additional, Wang, Xin M., additional, Ward, Michael D., additional, Ward‐Hartstonge, Kirsten A, additional, Warnatz, Klaus, additional, Warnes, Gary, additional, Warth, Sarah, additional, Waskow, Claudia, additional, Watson, James V., additional, Watzl, Carsten, additional, Wegener, Leonie, additional, Weisenburger, Thomas, additional, Wiedemann, Annika, additional, Wienands, Jürgen, additional, Wilharm, Anneke, additional, Wilkinson, Robert John, additional, Willimsky, Gerald, additional, Wing, James B., additional, Winkelmann, Rieke, additional, Winkler, Thomas H., additional, Wirz, Oliver F., additional, Wong, Alicia, additional, Wurst, Peter, additional, Yang, Jennie H. M., additional, Yang, Juhao, additional, Yazdanbakhsh, Maria, additional, Yu, Liping, additional, Yue, Alice, additional, Zhang, Hanlin, additional, Zhao, Yi, additional, Ziegler, Susanne Maria, additional, Zielinski, Christina, additional, Zimmermann, Jakob, additional, and Zychlinsky, Arturo, additional

Published
2019
Full Text
View/download PDF

18. Classical Type 1 Dendritic Cells Dominate Priming of Th1 Responses to Herpes Simplex Virus Type 1 Skin Infection

Author

Harpur, Christopher M., primary, Kato, Yu, additional, Dewi, Shinta T., additional, Stankovic, Sanda, additional, Johnson, Darryl N., additional, Bedoui, Sammy, additional, Whitney, Paul G., additional, Lahoud, Mireille H., additional, Caminschi, Irina, additional, Heath, William R., additional, Brooks, Andrew G., additional, and Gebhardt, Thomas, additional

Published
2019
Full Text
View/download PDF

20. Killer Immunoglobulin Receptor 3DL1-mediated recognition of Human Leukocyte Antigen B

Author

Vivian, Julian P., Duncan, Renee C., Berry, Richard, O’Connor, Geraldine M., Reid, Hugh H., Beddoe, Travis, Gras, Stephanie, Saunders, Philippa M., Olshina, Maya A., Widjaja, Jacqueline M.L., Harpur, Christopher M., Lin, Jie, Maloveste, Sebastien M., Price, David A., Lafont, Bernard A.P., McVicar, Daniel W., Clements, Craig S., Brooks, Andrew G., and Rossjohn, Jamie

Subjects
Models, Molecular, Structure-Activity Relationship, Binding Sites, Polymorphism, Genetic, HLA-B Antigens, Humans, Receptors, KIR3DL1, Mutant Proteins, Amino Acid Sequence, beta 2-Microglobulin, Article, Protein Structure, Tertiary
Abstract

Members of the Killer Immunoglobulin-Like Receptor (KIR) family, a large group of polymorphic receptors expressed on Natural Killer (NK) cells, recognise particular peptide-laden Human Leukocyte Antigen (pHLA) class I molecules and play a pivotal role in innate immune responses1. Allelic variation and extensive polymorphism within the three-domain KIR family (KIR3D, domains D0–D1–D2) affects pHLA binding specificity and is linked to the control of viral replication and the treatment outcome of certain haematological malignancies1–3. We describe the structure of the KIR3DL1 receptor, bound to HLA-B*5701 complexed with a self-peptide. KIR3DL1 clamped around the C-terminal end of the HLA-B*5701 antigen (Ag)-binding cleft, resulting in two discontinuous footprints on the pHLA. Firstly, the D0 domain, a distinguishing feature of the KIR3D family, extended towards β2-microglobulin and abutted a region of the HLA molecule that exhibited limited polymorphism, thereby acting as an “innate HLA sensor” domain. Secondly, while the D2-HLA-B*5701 interface exhibited a high degree of complementarity, the D1-pHLA-B*5701 contacts were sub-optimal and accommodated a degree of sequence variation both within the peptide and the polymorphic region of the HLA molecule. While the two-domain KIR (KIR2D) and KIR3DL1 docked similarly onto HLA-C4,5 and HLA-B respectively, the corresponding D1-mediated interactions differed markedly, thereby providing insight into the specificity of KIR3DL1 for discrete HLA-A and HLA-B allotypes. Collectively, in association with extensive mutagenesis studies at the KIR3DL1-pHLA B*5701 interface, we provide a framework for understanding the intricate interplay between peptide variability, KIR3D and HLA polymorphism in determining the specificity requirements of this essential innate interaction that is conserved across primate species.

Published
2011

22. Polymorphism in Human Cytomegalovirus UL40 Impacts on Recognition of Human Leukocyte Antigen-E (HLA-E) by Natural Killer Cells

Author

Heatley, Susan L., primary, Pietra, Gabriella, additional, Lin, Jie, additional, Widjaja, Jacqueline M.L., additional, Harpur, Christopher M., additional, Lester, Sue, additional, Rossjohn, Jamie, additional, Szer, Jeff, additional, Schwarer, Anthony, additional, Bradstock, Kenneth, additional, Bardy, Peter G., additional, Mingari, Maria Cristina, additional, Moretta, Lorenzo, additional, Sullivan, Lucy C., additional, and Brooks, Andrew G., additional

Published
2013
Full Text
View/download PDF

24. A Structural Basis for Antigen Presentation by the MHC Class Ib Molecule, Qa-1b.

Author

Li Zeng, Sullivan, Lucy C., Vivian, Julian P., Walpole, Nicholas G., Harpur, Christopher M., Rossjohn, Jamie, Clements, Craig S., and Brooks, Andrew G.

Subjects
*ANTIGENS, *T cells, *PEPTIDES, *HOMOLOGY (Biology), *LIGANDS (Biochemistry), *MOLECULES
Abstract

The primary function of the monomorphic MHC class Ib molecule Qa-1b is to present peptides derived from the leader sequences of other MHC class I molecules for recognition by the CD94-NKG2 receptors expressed by NK and T cells. Whereas the mode of peptide presentation by its ortholog HLA-E, and subsequent recognition by CD94-NKG2A, is known, the molecular basis of Qa-1b function is unclear. We have assessed the interaction between Qa-1b and CD94-NKG2A and shown that they interact with an affinity of 17 μM. Furthermore, we have determined the structure of Qa-1b bound to the leader sequence peptide, Qdm (AMAPRTLLL), to a resolution of 1.9 Å and compared it with that of HLA-E. The crystal structure provided a basis for understanding the restricted peptide repertoire of Qa-1b. Whereas the Qa-1b-AMAPRTLLL complex was similar to that of HLA-E, significant sequence and structural differences were observed between the respective Ag-binding clefts. However, the conformation of the Qdm peptide bound by Qa-1b was very similar to that of peptide bound to HLA-E. Although a number of conserved innate receptors can recognize heterologous ligands from other species, the structural differences between Qa-1b and HLA-E manifested in CD94-NKG2A ligand recognition being species specific despite similarities in peptide sequence and conformation. Collectively, our data illustrate the structural homology between Qa-1b and HLA-E and provide a structural basis for understanding peptide repertoire selection and the specificity of the interaction of Qa-1b with CD94-NKG2 receptors. [ABSTRACT FROM AUTHOR]

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results