1. Dual molecular imaging for targeting metalloproteinase activity and apoptosis in atherosclerosis: molecular imaging facilitates understanding of pathogenesis
- Author
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Haider, Nezam, Hartung, Dagmar, Fujimoto, Shinichiro, Petrov, Artiom, Kolodgie, Frank D., Virmani, Renu, Ohshima, Satoru, Liu, Han, Zhou, Jun, Fujimoto, Ai, Tahara, Atsuko, Hofstra, Leo, Narula, Navneet, Reutelingsperger, Chris, and Narula, Jagat
- Subjects
Medicine & Public Health ,Imaging / Radiology ,Nuclear Medicine ,Cardiology ,Radionuclides ,SPECT ,vulnerable atherosclerotic plaque - Abstract
Macrophage apoptosis and MMP activity contribute to vulnerability of atherosclerotic plaques to rupture. By employing molecular imaging techniques, we investigated if apoptosis and MMP release are interlinked.Atherosclerosis was produced in rabbits receiving high-cholesterol diet (HC), who underwent dual radionuclide imaging with 99mTc-labeled matrix metalloproteinase inhibitor (MPI) and 111In-labeled annexin A5 (AA5) using micro-SPECT/CT. %ID/g MPI and AA5 uptake was measured, followed by histological characterization. Unmanipulated animals were used as disease controls. Correlation between MPI and AA5 uptake was undertaken and relationship confirmed in culture study of activated THP-1 monocytes.MPI and AA5 uptake was best visualized in HC diet animals (n = 6) and reduced significantly after fluvastatin treatment (n = 4) or diet withdrawal (n = 3). %ID/g MPI (.087 ± .018%) and AA5 (.03 ± .01%) uptake was higher in HC than control (n = 6) animals (.014 ± .004%, P
- Published
- 2009