Search

Abstract: Atherosclerosis is considered to be a form of chronic inflammation governed by a complex network of inter- and intra-cellular signaling pathways. The pleiotropic cytokine interferon-γ (IFN-γ) is a key pro-inflammatory mediator that is expressed at high levels in atherosclerotic lesions. IFN-γ regulates the function and properties of all the cell types in the vessel wall. The precise role of IFN-γ in atherogenesis is complex, with both pro- and anti-atherogenic actions being identified. This review will discuss these actions of the cytokine along with recent findings that have emerged from mouse models of atherosclerosis that are deficient in IFN-γ signaling. [Copyright &y& Elsevier]

Abstract: Time-of-flight powder neutron diffraction has been performed on oxides with composition (La1− x Nd x )2Zr2O7 and Nd2(Zr1− x Ti x )2O7, where , 0.2, 0.4,…1.0, in order to determine the solid solution behaviour across each series. Between La2Zr2O7 and Nd2Zr2O7, a cubic pyrochlore phase is observed (, ). A linear decrease in the lattice parameter from 10.8047 to 10.6758Å indicates complete miscibility of the two end-members. For the same series, the oxygen x-parameter increases from 0.3313 to 0.3348, suggesting increased distortion of the 6 coordinate B sites and reduced distortion of the 8 coordinate sites. There is limited solubility of Nd2Ti2O7 in Nd2Zr2O7. Exsolution of a monoclinic phase (, ) rich in Nd2Ti2O7 is observed at approximately . The compositional range over which a solid solution exists is more extensive than that which has been previously reported. The solubility of Nd2Zr2O7 in Nd2Ti2O7 is very low. [Copyright &y& Elsevier]

The mechanisms underlying transcriptional inhibition by interferon-gamma (IFN-gamma) are poorly understood despite the existence of a large number of genes that are regulated in this manner and the key role of this cytokine in inflammatory disorders such as atherosclerosis. We have previously identified a novel mechanism for transcriptional inhibition by IFN-gamma that involves a reduction in the binding of transcription factors Sp1 and Sp3 to regulatory sequences in the lipoprotein lipase (LPL) gene. In the present study, we have investigated the signalling pathways that impact on the IFN-gamma-mediated regulation of Sp1/Sp3 binding and LPL gene transcription in macrophages. The IFN-gamma-mediated inhibition of LPL promoter activity was prevented by expression of dominant negative forms of casein kinase 2 (CK2) and protein kinase B (PKB), a key downstream component of the phosphoinositide-3-kinase (PI3K) pathway. IFN-gamma activated both the catalytic subunits of CK2 without affecting their expression. CK2 interacted with both Sp1 and Sp3 and this association was increased by IFN-gamma. Electrophoretic mobility shift assays showed that a CK2-mediated phosphorylation of either cellular extracts or recombinant Sp1 reduced binding to the regulatory region in the LPL gene. The action of PKB was potentially mediated through mammalian target for rapamycin proteins. Taken together, these results suggest a key role for CK2 and PI3K signalling pathways in the IFN-gamma-mediated inhibition of macrophage LPL gene transcription through the regulation of Sp1/Sp3 binding.

Objective: The interferon-gamma (IFN-gamma)-mediated regulation of macrophage gene expression is of crucial importance in the pathogenesis of atherosclerosis. The mechanisms underlying the actions of IFN-gamma signaling in macrophages were investigated using monocyte chemoattractant protein (MCP)-1 as a model gene., Methods and Results: The IFN-gamma-induced expression of MCP-1 in macrophages was attenuated by inhibitors of phosphoinositide-3-kinase (PI3K), casein kinase 2 (CK2), and Janus kinase (JAK)-2. AKT was the downstream target for PI3K action. Electrophoretic mobility shift assays and chromatin immunoprecipitation showed that signal transducer and activator of transcription (STAT)-1 interacted with IFN-gamma responsive elements in the MCP-1 gene promoter. The IFN-gamma-induced activity of the MCP-1 gene promoter and an artificial promoter containing STAT1 responsive elements was inhibited by expression of dominant negative forms of JAK-1 and -2, STAT1, CK2, and AKT. The action of CK2 and AKT on STAT1 activation was mediated, at least in part, through the regulation of serine 727 phosphorylation. Analysis of a number of other genes regulated by this cytokine and implicated in atherosclerosis revealed a gene-specific action for PI3K/AKT in IFN-gamma signaling., Conclusions: These studies provide novel insights into the role of PI3K/AKT and CK2 in IFN-gamma signaling relevant to changes in macrophage gene expression during atherosclerosis.