26 results on '"Hatfield, R. G."'
Search Results
2. A neotropical perspective on the uniqueness of the Holocene among interglacials
- Author
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Schiferl, J., Kingston, M., Åkesson, C. M., Valencia, B. G., Rozas-Davila, A., McGee, D., Woods, A., Chen, C. Y., Hatfield, R. G., Rodbell, D. T., Abbott, M. B., and Bush, M. B.
- Published
- 2023
- Full Text
- View/download PDF
3. 700,000 years of tropical Andean glaciation
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Rodbell, D. T., Hatfield, R. G., Abbott, M. B., Chen, C. Y., Woods, A., Stoner, J. S., McGee, D., Tapia, P. M., Bush, M., Valero-Garcés, B. L., Lehmann, S. B., Mark, S. Z., Weidhaas, N. C., Hillman, A. L., Larsen, D. J., Delgado, G., Katz, S. A., Solada, K. E., Morey, A. E., Finkenbinder, M., Valencia, B., Rozas-Davila, A., Wattrus, N., Colman, S. M., Bustamante, M. G., Kück, J., and Pierdominici, S.
- Published
- 2022
- Full Text
- View/download PDF
4. Stratigraphic correlation and splice generation for sediments recovered from a large-lake drilling project: an example from Lake Junín, Peru
- Author
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Hatfield, R. G., Woods, A., Lehmann, S. B., Weidhaas, N., Chen, C. Y., Kück, J., Pierdominici, S., Stoner, J. S., Abbott, M. B., and Rodbell, D. T.
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- 2020
- Full Text
- View/download PDF
5. Stratigraphic correlation and splice generation for sediments recovered from a large-lake drilling project: an example from Lake Junín, Peru
- Author
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Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences, Hatfield, R. G., Woods, A., Lehmann, S. B., Weidhaas, N., Chen, C. Y., Kück, J., Pierdominici, S., Stoner, J. S., Abbott, M. B., Rodbell, D. T., Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences, Hatfield, R. G., Woods, A., Lehmann, S. B., Weidhaas, N., Chen, C. Y., Kück, J., Pierdominici, S., Stoner, J. S., Abbott, M. B., and Rodbell, D. T.
- Abstract
Sediment records from deep-drilling projects such as those carried out by the International Continental Scientific Drilling Program are often tens to hundreds of meters in length. To ensure the complete recovery of a stratigraphic section, a basin is usually cored multiple times in adjacent holes so that gaps between sequential cores, poorly recovered sections, or intervals affected by disturbance can be bridged or replaced with sediments from another hole. Stratigraphic correlation, the alignment of stratigraphically-equivalent horizons in cores from different holes in a common-depth scale, and splice generation, the integration of the most-representative core sections into a composite-stratigraphic section, are essential steps in this process to both evaluate and synthesize the recovered-sediment record and focus the scientific analyses. However, these undertakings can be complex and are inherently subjective, making the need for the development of a single robust stratigraphic section early in the project critical to its success. Despite this, the steps between core recovery and on-splice data generation are rarely published in sufficient detail to allow reconstruction, or refinement, of the composited record at a later date. To increase the transparency of how the composite record is created, and to provide a template for future projects, we detail the step-by-step approaches and decisions involved in generating the composite-depth scale and complete-stratigraphic splice following recovery of sediments from Lake Junín, Peru. We first explain the details and nuances of different drilling-depth scales before describing how we integrated different physical property records to generate the composite-depth scale and complete-stratigraphic splice. Here, we show that due to the complex stratigraphy in the Lake Junín sediments, high-resolution line-scan images of the cores offer millimeter-scale precision for construction of the primary-stratigraphic splice at
- Published
- 2022
6. 700,000 years of tropical Andean glaciation
- Author
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National Science Foundation (US), Valero-Garcés, Blas L. [0000-0003-2214-7057], Rodbell, D. T., Hatfield, R. G., Abbott, Mark B., Chen, C. Y., Woods, A., Stoner, J. S., McGee, D., Tapia, P. M., Bush, M., Valero-Garcés, Blas L., Lehmann, S. B., Mark, S. Z., Weidhaas, N. C., Hillman, Aubrey L., Larsen, D. J., Delgado, G., Katz, S. A., Solada, K. E., Morey, A. E., Finkenbinder, M., Valencia, B., Rozas-Davila, A., Wattrus, N., Colman, S. M., Bustamante, M. G., Kück, J., Pierdominici, S., National Science Foundation (US), Valero-Garcés, Blas L. [0000-0003-2214-7057], Rodbell, D. T., Hatfield, R. G., Abbott, Mark B., Chen, C. Y., Woods, A., Stoner, J. S., McGee, D., Tapia, P. M., Bush, M., Valero-Garcés, Blas L., Lehmann, S. B., Mark, S. Z., Weidhaas, N. C., Hillman, Aubrey L., Larsen, D. J., Delgado, G., Katz, S. A., Solada, K. E., Morey, A. E., Finkenbinder, M., Valencia, B., Rozas-Davila, A., Wattrus, N., Colman, S. M., Bustamante, M. G., Kück, J., and Pierdominici, S.
- Abstract
Our understanding of the climatic teleconnections that drove ice-age cycles has been limited by a paucity of well-dated tropical records of glaciation that span several glacial–interglacial intervals. Glacial deposits offer discrete snapshots of glacier extent but cannot provide the continuous records required for detailed interhemispheric comparisons. By contrast, lakes located within glaciated catchments can provide continuous archives of upstream glacial activity, but few such records extend beyond the last glacial cycle. Here a piston core from Lake Junín in the uppermost Amazon basin provides the first, to our knowledge, continuous, independently dated archive of tropical glaciation spanning 700,000 years. We find that tropical glaciers tracked changes in global ice volume and followed a clear approximately 100,000-year periodicity. An enhancement in the extent of tropical Andean glaciers relative to global ice volume occurred between 200,000 and 400,000 years ago, during sustained intervals of regionally elevated hydrologic balance that modified the regular approximately 23,000-year pacing of monsoon-driven precipitation. Millennial-scale variations in the extent of tropical Andean glaciers during the last glacial cycle were driven by variations in regional monsoon strength that were linked to temperature perturbations in Greenland ice cores1; these interhemispheric connections may have existed during previous glacial cycles.
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- 2022
7. Relative Paleointensity Record of Integrated Ocean Drilling Program Site U1396 in the Caribbean Sea: Geomagnetic and Chronostratigraphic Observations in the Pliocene
- Author
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Hatfield, R. G., primary, Stoner, J. S., additional, and Fraass, A. J., additional
- Published
- 2021
- Full Text
- View/download PDF
8. Stratigraphic correlation and splice generation for sediments recovered from a large-lake drilling project: an example from Lake Junín, Peru
- Author
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Hatfield, R. G, Woods, A., Lehmann, S. B, Weidhaas, N., Chen, C. Y, Kück, J., Pierdominici, S., Stoner, J. S, Abbott, M. B, Rodbell, D. T, Hatfield, R. G, Woods, A., Lehmann, S. B, Weidhaas, N., Chen, C. Y, Kück, J., Pierdominici, S., Stoner, J. S, Abbott, M. B, and Rodbell, D. T
- Abstract
Sediment records from deep-drilling projects such as those carried out by the International Continental Scientific Drilling Program are often tens to hundreds of meters in length. To ensure the complete recovery of a stratigraphic section, a basin is usually cored multiple times in adjacent holes so that gaps between sequential cores, poorly recovered sections, or intervals affected by disturbance can be bridged or replaced with sediments from another hole. Stratigraphic correlation, the alignment of stratigraphically-equivalent horizons in cores from different holes in a common-depth scale, and splice generation, the integration of the most-representative core sections into a composite-stratigraphic section, are essential steps in this process to both evaluate and synthesize the recovered-sediment record and focus the scientific analyses. However, these undertakings can be complex and are inherently subjective, making the need for the development of a single robust stratigraphic section early in the project critical to its success. Despite this, the steps between core recovery and on-splice data generation are rarely published in sufficient detail to allow reconstruction, or refinement, of the composited record at a later date. To increase the transparency of how the composite record is created, and to provide a template for future projects, we detail the step-by-step approaches and decisions involved in generating the composite-depth scale and complete-stratigraphic splice following recovery of sediments from Lake Junín, Peru. We first explain the details and nuances of different drilling-depth scales before describing how we integrated different physical property records to generate the composite-depth scale and complete-stratigraphic splice. Here, we show that due to the complex stratigraphy in the Lake Junín sediments, high-resolution line-scan images of the cores offer millimeter-scale precision for construction of the primary-stratigraphic splice at
- Published
- 2021
9. Stratigraphic correlation and splice generation for sediments recovered from a large-lake drilling project: an example from Lake Junín, Peru
- Author
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Hatfield, R. G., primary, Woods, A., additional, Lehmann, S. B., additional, Weidhaas, N., additional, Chen, C. Y., additional, Kück, J., additional, Pierdominici, S., additional, Stoner, J. S., additional, Abbott, M. B., additional, and Rodbell, D. T., additional
- Published
- 2019
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- View/download PDF
10. The Malt House Ruins
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Hatfield, R. G.
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- 1858
11. Site U1490.
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Rosenthal, Y., Holbourn, A. E., Kulhanek, D. K., Aiello, I. W., Babila, T. L., Bayon, G., Beaufort, L., Bova, S. C., Chun, J.-H., Dang, H., Drury, A. J., Jones, T. Dunkley, Eichler, P. P. B., Fernando, A. G. S., Gibson, K., Hatfield, R. G., Johnson, D. L., Kumagai, Y., Li, T., and Linsley, B. K.
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OCEANOGRAPHY ,PLEISTOCENE Epoch ,CYCLOSTRATIGRAPHY ,PALEOMAGNETISM - Published
- 2018
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- View/download PDF
12. Expedition 363 summary.
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Rosenthal, Y., Holbourn, A. E., Kulhanek, D. K., Aiello, I. W., Babila, T. L., Bayon, G., Beaufort, L., Bova, S. C., Chun, J.-H., Dang, H., Drury, A. J., Jones, T. Dunkley, Eichler, P. P. B., Fernando, A. G. S., Gibson, K., Hatfield, R. G., Johnson, D. L., Kumagai, Y., Li, T., and Linsley, B. K.
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OCEANOGRAPHY ,CLIMATE change ,BIOSTRATIGRAPHY ,PALEOMAGNETISM ,FOSSIL foraminifera - Abstract
International Ocean Discovery Program Expedition 363 sought to document the regional expression and driving mechanisms of climate variability (e.g., temperature, precipitation, and productivity) in the Indo-Pacific Warm Pool (IPWP) as it relates to the evolution of Neogene climate on millennial, orbital, and geological timescales. To achieve our objectives, we selected sites with a wide geographical distribution and variable oceanographic and depositional settings. Nine sites were cored during Expedition 363, recovering a total of 6956 m of sediment in 875-3421 m water depth with an average recovery of 101.3% during 39.6 days of on-site operations. Two moderate sedimentation rate (~3-10 cm/ky) sites are located off northwestern Australia at the southwestern maximum extent of the IPWP and span the late Miocene to present. Seven of the nine sites are situated at the heart of the Western Pacific Warm Pool (WPWP), including two sites on the northern margin of Papua New Guinea with very high sedimentation rates (>60 cm/ky) spanning the past ~450 ky, two sites in the Manus Basin (north of Papua New Guinea) with moderate sedimentation rates (~4-14 cm/ky) recovering upper Pliocene to present sequences, and three sites with low sedimentation rates (~1-3 cm/ky) on the southern and northern Eauripik Rise spanning the early Miocene to present. The wide spatial distribution of the cores, variable accumulation rates, exceptional biostratigraphic and paleomagnetic age constraints, and mostly excellent or very good foraminifer preservation will allow us to trace the evolution of the IPWP through the Neogene at different temporal resolutions, meeting the primary objectives of Expedition 363. Specifically, the high-sedimentation rate cores off Papua New Guinea will allow us to better constrain mechanisms influencing millennial-scale variability in the WPWP, their links to high-latitude climate variability, and implications for temperature and precipitation in this region under variable mean-state climate conditions. Furthermore, the high accumulation rates offer the opportunity to study climate variability during previous warm periods at a resolution similar to that of existing studies of the Holocene. With excellent recovery, Expedition 363 sites are suitable for detailed paleoceanographic reconstructions at orbital and suborbital resolution from the middle Miocene to Pleistocene and thus will be used to refine the astronomical tuning, biostratigraphy, magnetostratigraphy, and isotope stratigraphy of hitherto poorly constrained intervals within the Neogene timescale (e.g., the late Miocene) and to reconstruct the history of the Asian-Australian monsoon and the Indonesian Throughflow on orbital and tectonic timescales. Results from high-resolution interstitial water sampling at selected sites will be used to reconstruct density profiles of the western equatorial Pacific deep water during the Last Glacial Maximum. Additional geochemical analyses of interstitial water samples in this tectonically active region will be used to investigate volcanogenic mineral and carbonate weathering and their possible implications for the evolution of Neogene climate. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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13. Expedition 363 methods.
- Author
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Rosenthal, Y., Holbourn, A. E., Kulhanek, D. K., Aiello, I. W., Babila, T. L., Bayon, G., Beaufort, L., Bova, S. C., Chun, J.-H., Dang, H., Drury, A. J., Jones, T. Dunkley, Eichler, P. P. B., Fernando, A. G. S., Gibson, K., Hatfield, R. G., Johnson, D. L., Kumagai, Y., Li, T., and Linsley, B. K.
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SCIENTISTS ,PALEONTOLOGISTS ,GEOCHEMISTS - Published
- 2018
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14. Submarine record of volcanic island construction and collapse in the Lesser Antilles arc: First scientific drilling of submarine volcanic island landslides by IODP Expedition 340
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Le Friant, A., Ishizuka, O., Boudon, G., Palmer, M. R., Talling, P. J., Villemant, B., Adachi, T., Aljahdali, M., Breitkreuz, C., Brunet, M., Caron, B., Coussens, M., Deplus, C., Endo, D., Feuillet, N., Fraas, A. J., Fujinawa, A., Hart, M. B., Hatfield, R. G., Hornbach, M., Jutzeler, M., Kataoka, K. S., Komorowski, J.-C., Lebas, Elodie, Lafuerza, S., Maeno, F., Manga, M., Martínez-Colón, M., McCanta, M., Morgan, S., Saito, T., Slagle, A., Sparks, S., Stinton, A., Stroncik, N., Subramanyam, K. S. V., Tamura, Y., Trofimovs, J., Voight, B., Wall-Palmer, D., Wang, F., Watt, S. F. L., Le Friant, A., Ishizuka, O., Boudon, G., Palmer, M. R., Talling, P. J., Villemant, B., Adachi, T., Aljahdali, M., Breitkreuz, C., Brunet, M., Caron, B., Coussens, M., Deplus, C., Endo, D., Feuillet, N., Fraas, A. J., Fujinawa, A., Hart, M. B., Hatfield, R. G., Hornbach, M., Jutzeler, M., Kataoka, K. S., Komorowski, J.-C., Lebas, Elodie, Lafuerza, S., Maeno, F., Manga, M., Martínez-Colón, M., McCanta, M., Morgan, S., Saito, T., Slagle, A., Sparks, S., Stinton, A., Stroncik, N., Subramanyam, K. S. V., Tamura, Y., Trofimovs, J., Voight, B., Wall-Palmer, D., Wang, F., and Watt, S. F. L.
- Abstract
IODP Expedition 340 successfully drilled a series of sites offshore Montserrat, Martinique and Dominica in the Lesser Antilles from March to April 2012. These are among the few drill sites gathered around volcanic islands, and the first scientific drilling of large and likely tsunamigenic volcanic island-arc landslide deposits. These cores provide evidence and tests of previous hypotheses for the composition and origin of those deposits. Sites U1394, U1399, and U1400 that penetrated landslide deposits recovered exclusively seafloor-sediment, comprising mainly turbidites and hemipelagic deposits, and lacked debris avalanche deposits. This supports the concepts that i/ volcanic debris avalanches tend to stop at the slope break, and ii/ widespread and voluminous failures of pre-existing low-gradient seafloor sediment can be triggered by initial emplacement of material from the volcano. Offshore Martinique (U1399 and 1400), the landslide deposits comprised blocks of parallel strata that were tilted or micro-faulted, sometimes separated by intervals of homogenized sediment (intense shearing), while Site U1394 offshore Montserrat penetrated a flat-lying block of intact strata. The most likely mechanism for generating these large-scale seafloor-sediment failures appears to be propagation of a decollement from proximal areas loaded and incised by a volcanic debris avalanche. These results have implications for the magnitude of tsunami generation. Under some conditions, volcanic island landslide deposits comprised of mainly seafloor sediment will tend to form smaller magnitude tsunamis than equivalent volumes of subaerial block-rich mass flows rapidly entering water. Expedition 340 also successfully drilled sites to access the undisturbed record of eruption fallout layers intercalated with marine sediment which provide an outstanding high-resolution dataset to analyze eruption and landslides cycles, improve understanding of magmatic evolution as well as offshore sedimentatio
- Published
- 2015
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15. Submarine record of volcanic island construction and collapse in the Lesser Antilles arc: First scientific drilling of submarine volcanic island landslides by IODPExpedition 340
- Author
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Le Friant, A., primary, Ishizuka, O., additional, Boudon, G., additional, Palmer, M. R., additional, Talling, P. J., additional, Villemant, B., additional, Adachi, T., additional, Aljahdali, M., additional, Breitkreuz, C., additional, Brunet, M., additional, Caron, B., additional, Coussens, M., additional, Deplus, C., additional, Endo, D., additional, Feuillet, N., additional, Fraas, A. J., additional, Fujinawa, A., additional, Hart, M. B., additional, Hatfield, R. G., additional, Hornbach, M., additional, Jutzeler, M., additional, Kataoka, K. S., additional, Komorowski, J.‐C., additional, Lebas, E., additional, Lafuerza, S., additional, Maeno, F., additional, Manga, M., additional, Martínez‐Colón, M., additional, McCanta, M., additional, Morgan, S., additional, Saito, T., additional, Slagle, A., additional, Sparks, S., additional, Stinton, A., additional, Stroncik, N., additional, Subramanyam, K. S. V., additional, Tamura, Y., additional, Trofimovs, J., additional, Voight, B., additional, Wall‐Palmer, D., additional, Wang, F., additional, and Watt, S. F. L., additional
- Published
- 2015
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16. Grand Central Depot, 1890.
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Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, Buckhout, Isaac C., Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, and Buckhout, Isaac C.
- Abstract
East 42nd Street and Park Avenue North. Grand Central Depot, 1890. Grand Central Depot opened in 1871 for the New York and Harlem Railroad and the New Haven Railroad. Architect: John B. Snook in association with engineer Isaac C. Buckhout. Original glass and metal train shed designed by R.G. Hatfield. Snook and Buckhout's designed the depot in the American Second Empire Style using red pressed brick and cast iron trim. The train shed was razed in 1903 when construction for the new Grand Central Terminal began.
- Published
- 1890
17. View of Grand Central Depot from Lexington Avenue, 1890.
- Author
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Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, Buckhout, Isaac C., Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, and Buckhout, Isaac C.
- Abstract
Grand Central Depot, East 42nd Street and Park Avenue North, 1890. Grand Central Depot opened in 1871 for the New York and Harlem Railroad and the New Haven Railroad. Architect: John B. Snook in association with engineer Isaac C. Buckhout. Original glass and metal train shed designed by R.G. Hatfield. Snook and Buckhout designed the depot in the American Second Empire Style using red pressed brick and cast iron trim. The train shed was razed in 1903 when construction began for the new Grand Central Terminal.
- Published
- 1890
18. Street cars, Grand Central Depot, East 42nd Street and Park Avenue North, 1890.
- Author
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Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, Buckhout, Isaac C., Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, and Buckhout, Isaac C.
- Abstract
View looking west along 42nd Street. Grand Central Depot is to the right. Street cars run along the street past the station. Snook and Buckhout's designed the depot in the American Second Empire Style using red pressed brick and cast iron trim. The train shed was razed in 1903 when construction for the new Grand Central Terminal began.
- Published
- 1890
19. The American house-carpenter; a treatise upon architecture, cornices and mouldings, framing, doors, windows, and stairs. Together with the most important principles of practical geometry. By R.G. Hatfield.
- Author
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Hatfield, R. G., Hatfield, R.G. (Robert Griffith), 1815-1879., Hatfield, R. G., and Hatfield, R.G. (Robert Griffith), 1815-1879.
- Abstract
We have determined this item to be in the public domain according to US copyright law through information in the bibliographic record and/or US copyright renewal records. The digital version is available for all educational uses worldwide. Please contact HathiTrust staff at hathitrust-help@umich.edu with any questions about this item., Carpentry., Architecture., (LCCN)08003700., (OCoLC)3335777., Sdr-ia-srlf218599., TH5604 .H36., Http://hdl.handle.net/2027/uc2.ark:/13960/t2g737s9s.
20. The American house-carpenter; a treatise on the art of building, and the strength of materials. By R.G. Hatfield.
- Author
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Hatfield, R. G., Hatfield, R.G. (Robert Griffith), 1815-1879., Hatfield, R. G., and Hatfield, R.G. (Robert Griffith), 1815-1879.
- Abstract
We have determined this item to be in the public domain according to US copyright law through information in the bibliographic record and/or US copyright renewal records. The digital version is available for all educational uses worldwide. Please contact HathiTrust staff at hathitrust-help@umich.edu with any questions about this item., Carpentry., Architecture., (LCCN)32025964., (OCoLC)361411719., Sdr-ia-srlf218598., TH5604 .H35 1867., Http://hdl.handle.net/2027/uc2.ark:/13960/t0cv4cf54.
21. Grand Central Depot, 1870's.
- Author
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Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, Buckhout, Isaac C., Snook, John B. (John Butler), 1815-1901, Hatfield, R. G. (Robert Griffith), 1815-1879, and Buckhout, Isaac C.
- Abstract
East 42nd Street and Park Avenue North. Grand Central Depot - 1870's. Grand Central Depot opened in 1871 for the New York and Harlem Railroad and the New Haven Railroad. Architect: John B. Snook in association with engineer Isaac C. Buckhout. Original glass and metal train shed designed by R.G. Hatfield. Snook and Buckhout's designed the depot in the American Second Empire Style using red pressed brick and cast iron trim. The train shed was razed in 1903 when construction for the new Grand Central Terminal began.
22. Anti-Fire Construction
- Author
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Hatfield, R. G., primary
- Published
- 1879
- Full Text
- View/download PDF
23. The American House-Carpenter
- Author
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Hatfield, R. G., primary
- Published
- 1857
- Full Text
- View/download PDF
24. NOTE ON THE LEVER.
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HATFIELD, R. G.
- Published
- 1858
25. Items for Model Cottage.
- Author
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HATFIELD, R. G
- Published
- 1854
26. Application of Six Detection Methods for Analysis of Paralytic Shellfish Toxins in Shellfish from Four Regions within Latin America
- Author
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Andrew D. Turner, Alejandra B. Goya, Frances M. Van Dolah, Maria Salhi, Benjamin A. Suarez-Isla, Robert G. Hatfield, Maggie Broadwater, Dinorah Medina, Fernanda Barrera, Mickael Teixeira-Alves, Ignacio Rubilar, D. Carrasco, Sophie Tarnovius, Ernesto García-Mendoza, Turner A. D., Tarnovius S., Hatfield R. G., Alves M. T., Broadwater M., Van Dolah F., Garcia-Mendoza E., Medina Dinorah, DINARA, Salhi María, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Ecología y Ciencias Ambientales. DINARA, Goya A. B., Barrera F., Carrasco D., Rubilar I., and Suarez-Isla B. A.
- Subjects
0106 biological sciences ,toxin profiles ,Latin Americans ,MBA ,Pharmaceutical Science ,Biology ,RBA ,01 natural sciences ,Article ,Mice ,Mouse bioassay ,Paralytic shellfish poisoning (PSP) ,Tandem Mass Spectrometry ,Toxicity Tests ,Drug Discovery ,Lc ms ms ,medicine ,Animals ,Paralysis ,Shellfish Poisoning ,False Positive Reactions ,paralytic shellfish poisoning (PSP) ,LC-MS/MS ,Paralytic shellfish poisoning ,lcsh:QH301-705.5 ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Chromatography, High Pressure Liquid ,Shellfish ,Replacement method ,business.industry ,010604 marine biology & hydrobiology ,010401 analytical chemistry ,Reproducibility of Results ,Reference Standards ,Toxin profiles ,medicine.disease ,Bivalvia ,0104 chemical sciences ,Shellfish poisoning ,Biotechnology ,Latin America ,lcsh:Biology (General) ,Biological Assay ,Marine Toxins ,LC-FLD ,business - Abstract
With the move away from use of mouse bioassay (MBA) to test bivalve mollusc shellfish for paralytic shellfish poisoning (PSP) toxins, countries around the world are having to adopt non-animal-based alternatives that fulfil ethical and legal requirements. Various assays have been developed which have been subjected to single-laboratory and multi-laboratory validation studies, gaining acceptance as official methods of analysis and approval for use in some countries as official control testing methods. The majority of validation studies conducted to date do not, however, incorporate shellfish species sourced from Latin America. Consequently, this study sought to investigate the performance of five alternative PSP testing methods together with the MBA, comparing the PSP toxin data generated both qualitatively and quantitatively. The methods included a receptor binding assay (RBA), two liquid chromatography with fluorescence detection (LC-FLD) methods including both pre-column and post-column oxidation, liquid chromatography with tandem mass spectrometry (LC-MS/MS) and a commercial lateral flow assay (LFA) from Scotia. A total of three hundred and forty-nine shellfish samples from Argentina, Mexico, Chile and Uruguay were assessed. For the majority of samples, qualitative results compared well between methods. Good statistical correlations were demonstrated between the majority of quantitative results, with a notably excellent correlation between the current EU reference method using pre-column oxidation LC-FLD and LC-MS/MS. The LFA showed great potential for qualitative determination of PSP toxins, although the findings of high numbers of false-positive results and two false negatives highlighted that some caution is still needed when interpreting results. This study demonstrated that effective replacement methods are available for countries that no longer wish to use the MBA, but highlighted the importance of comparing toxin data from the replacement method using local shellfish species of concern before implementing new methods in official control testing programs.
- Published
- 2020
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