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237 results on '"Hattori, Masa-Aki"'

12. Gene silencing of myostatin in differentiation of chicken embryonic myoblasts by small interfering RNA

Author

Sato, Fuminori, Kurokawa, Masatoshi, Yamauchi, Nobuhiko, and Hattori, Masa-aki

Subjects
Chick embryo -- Research, Chick embryo -- Genetic aspects, Gene silencing -- Research, Biological sciences
Abstract

Myostatin (GDF-8) is known to negatively regulate skeletal muscle mass in myogenesis, but few studies have been conducted on the function of endogenous GDF-8 in primary myoblasts. The present study was performed to assess the function of GDF-8 by RNA interference using primary culture of chicken embryonic myoblasts in which myoblasts were differentiated into myotubes. An active form of small interfering RNA (siRNA-1) targeting GDF-8 mRNA was introduced into myoblasts, and an inactive form of siRNA (siRNA-2) was used as a negative control. GDF-8 transcript level was significantly reduced 24 h after the introduction of siRNA-1 to 25% of the control, whereas a 52-kDa GDF-8 precursor was reduced to 45% of the control at 48 h. However, siRNA-2 did not decrease GDF-8 transcript level. When GDF-8-mediated promoter activity was measured chronologically by means of a pGL(CAGA)w-constructed luciferase reporter assay, a concomitant change in activity was initiated after 24 h. The activity rapidly decreased 30 h after siRNA-1 introduction, whereas high activity was maintained at 30-42 h in the control and siRNA-2-treated myoblasts. Myogenic factors such as MyoD and p21, but not myogenin, were altered after 72 h. Cell fusion of the multinucleated myotubes was delayed by the siRNA-1 introduction, and myotubes with aggregated nuclei were shorter and wider. These results strongly suggest that deficiency of GDF-8 delays cell differentiation and causes great alterations in the cellular morphology of chicken embryonic myotubes. GDF-8; GDF-8-mediated promoter activity; multinucleated myotubes doi:10.1152/ajpcell.00543.2005

Published
2006

19. Nitric oxide: a modulator for the epidermal growth factor receptor expression in developing ovarian granulosa cells

Author

Hattori, Masa-Aki, Sakamoto, Kenji, Fujihara, Noboru, and Kojima, Itaru

Subjects
Epidermal growth factor -- Physiological aspects, Nitric oxide -- Physiological aspects, Gene expression -- Physiological aspects, Genetic regulation -- Physiological aspects, Ovaries -- Physiological aspects, Biological sciences
Abstract

The role of nitric oxide (NO) in the regulation of epidermal growth factor (EGF) expression during differentiation of rat granulosa cells was investigated. Cultured cells showed a marked increase in guanosine 3',5'-cyclic monophosphate during development, but this was blocked by NO synthase inhibitors. The addition of L-arginine reversed this effect, suggesting that L-arginine is a potent suppressor of the inhibitory effects of NO synthase. Furthermore, exposure of the cells to sodium nitroprusside, an NO donor, increased EGF levels, indicating that NO is involved in EGF expression during cell differentiation.

Published
1996

36. The nuclear receptor REV- ERB α represses the transcription of growth/differentiation factor 10 and 15 genes in rat endometrium stromal cells.

Author

Zhao, Lijia, Isayama, Keishiro, Chen, Huatao, Yamauchi, Nobuhiko, Shigeyoshi, Yasufumi, Hashimoto, Seiichi, and Hattori, Masa‐aki

Subjects
NUCLEAR receptors (Biochemistry), GROWTH factors, GENE expression, BIOLUMINESCENCE, STROMAL cells
Abstract

Cellular oscillators in the uterus play critical roles in the gestation processes of mammals through entraining of the clock proteins to numerous downstream genes, including growth/differentiation factor ( Gdf) 10 and Gdf15. The expression of Gdf10 and Gdf15 is significantly increased in the uterus during decidualization, but the mechanism underlying the regulation of Gdf gene expression in the uterus is poorly understood. Here, we focused on the function of the cellular oscillators in the expression of Gdf family by using uterine endometrial stromal cells ( UESCs) isolated from pregnant Per2- dLuc transgenic rats. A significant decline of Per2- dLuc bioluminescence activity was induced in in vitro decidualized UESCs, and concomitantly the expression of canonical clock genes was downregulated. Conversely, the expression of Gdf10 and Gdf15 of the Gdf was upregulated. In UESCs transfected with Bmal1-specific si RNA, in which Rev-erbα expression was downregulated, Gdf10 and Gdf15 were upregulated. However, Gdf5, Gdf7, and Gdf11 were not significantly affected by Bmal1 silencing. The expression of Gdf10 and Gdf15 was enhanced after treatment with a REV- ERB α antagonist in the presence or absence of progesterone. Chromatin immunoprecipitation- PCR analysis revealed the inhibitory effect of REV- ERB α on the expression of Gdf10 and Gdf15 in UESCs by recognizing their gene promoters. Collectively, our findings indicate that the attenuation of REV- ERB α leads to an upregulation of Gdf10 and Gdf15 in decidual cells, in which cellular oscillators are impaired. Our results provide novel evidence regarding the functions of cellular oscillators regulating the expression of downstream genes during the differentiation of UESCs. [ABSTRACT FROM AUTHOR]

Published
2016
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50. In vitro decidualization of rat endometrial stromal cells

Author

Matsumoto, Kenji, primary, Yamauchi, Nobuhiko, additional, Watanabe, Ryo, additional, Oozono, Shinji, additional, Kubota, Kaiyu, additional, Nishimura, Kyohei, additional, Wood, Chris, additional, Soh, Tomoki, additional, Kizaki, Kei-ichirou, additional, and Hattori, Masa-aki, additional

Published
2008
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