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Domestication - the artificial selection of wild species to obtain variants with traits of human interest - was integral to the rise of complex societies. The oversupply of food was probably associated with the formalization of food preservation strategies through microbial fermentation. While considerable literature exists on the antiquity of fermented food, only few eukaryotic microbes have been studied so far for signs of domestication, less is known for bacteria. Here, we tested if cheese starter cultures harbour typical hallmarks of domestication by characterising over 100 community samples and over 100 individual strains isolated from historical and modern traditional Swiss cheese starter cultures. We find that cheese starter cultures have low genetic diversity both at the species and strain-level and maintained stable phenotypic traits. Molecular clock dating further suggests that the evolutionary origin of the bacteria approximately coincided with the first archaeological records of cheese making. Finally, we find evidence for ongoing genome decay and pseudogenization via transposon insertion related to a reduction of their niche breadth. Future work documenting the prevalence of these hallmarks across diverse fermented food systems and geographic regions will be key to unveiling the joint history of humanity with fermented food microbes., (© 2024. The Author(s).)

Background: The evolution of retention forces in one-piece mini dental implants (MDIs) retaining implant overdentures (IODs) is of major importance, as the male parts cannot be exchanged, due to the implant design., Purpose: To report the evolution of retention forces of one-piece MDIs, retaining mandibular IODs with ball/ O-ring attachments during 5 years assessed in vivo., Materials and Methods: Four MDIs were installed in the interforaminal region and immediately loaded using the existing mandibular complete denture. Directly post-op, at the 1-year and the 5-year follow-up, the retention forces were assessed with a validated strain gauge at each implant site separately., Results: At the male part, changes could only be observed at implant site 34: During the first year, there was a statistically significant increase, whereas the retention forces were decreased at the 5-year follow-up. At the female part, retention forces decreased significantly over time. Baseline values could be reestablished by exchanging the O-rings. The changes of the retention forces were more obvious in the posterior, compared to the anterior implants., Conclusions: Retention forces at the female part decrease significantly over time, when retaining mandibular IODs by MDIs with ball/ O-ring attachments. Baseline values can be reestablished by exchanging the O-rings. At the male part, changes of retention forces depend on the implant location., (© 2019 Wiley Periodicals, Inc.)

Objective: To investigate in a prospective cohort study the 5-year post-loading survival and success of one-piece mini dental implants (MDIs) in edentulous subjects with mandibular implant overdentures (IODs) and to report the associated changes of oral function with respect to patient age., Materials and Methods: Independently living edentulous patients were recruited and provided with new complete dentures. After an adaptation period, four one-piece MDIs (diameter 1.8 mm) were installed in the interforaminal region and immediately loaded. At baseline pre-operative (BL), as well as at 1-year and 5-year follow-up examinations, chewing efficiency was assessed with a validated color-mixing ability test and maximum voluntary bite force (MBF) was recorded with a digital force gauge. Implant survival and success were evaluated at 5-year follow-up. Non-parametric tests served to analyze the differences between time points., Results: Twenty patients participated in the study (5 men and 15 women; age at BL: n = 10 ≤ 65 years and n = 10 > 65 years). All patients were available for a 5-year follow-up (n = 2 in their long-term care facility; n = 1 only by telephone). The survival and success rates were both 100% after 61 ± 5.7 months. Chewing efficiency did not change over the first year (p = 0.167), but was improved at 5 year fup (n = 19) compared to baseline (p = 0.033) and to 1 year (p < 0.001). The MBF (n = 19 at 5-year follow-up) increased continuously over time (p < 0.001), but was less pronounced in the older cohort (p = 0.009)., Conclusions: Mini dental implants seem to be a successful treatment option for edentulous elderly patients with very high survival and success rates, and serve to improve long-term oral function., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Objectives: The aim of this study was to assess long-term changes in oral health-related quality of life (OHRQoL) over a period of 5 years in patients treated with narrow diameter implants (NDI) in the mandible for support of an overdenture., Methods: In this prospective clinical study, a consecutive sample of 20 edentulous patients who had worn sufficient complete dentures for at least 12 weeks was provided with four immediately loaded one-piece titanium NDIs in the mandible. The German 49-item version of the Oral Health Impact Profile (OHIP) was applied to assess OHRQoL at baseline and all follow-ups (4 and 8 weeks, 3, 6, and 12 months, and 3 and 5 years after treatment was finished)., Results: Before treatment, OHRQoL was substantially impaired indicated by high OHIP summary score (39.9 points). Four weeks after treatment, a substantial treatment-induced drop of OHRQoL impairment was observed (21.8 OHIP points), and at all follow-ups, OHIP scores were lower than at baseline (15.9-26.5 OHIP points; ANOVA: p<.001). Based on mixed-effect linear regression analyses, the treatment-induced effect on OHRQoL improvement over the entire study period was statistically significant for the OHIP summary scores as well as for all four OHIP domains: Oral Function, Orofacial Pain, Orofacial Appearance, and Psychosocial Impact (all: p<.001)., Conclusions: This study suggests that four immediately loaded NDIs for the support of conventional complete dentures in edentulous patients with substantially impaired OHRQoL may lead to a long-lasting treatment-induced improvement in OHRQoL., Clinical Significance: The provision of four NDI in the edentulous mandible is a promising treatment option for patients with substantial OHRQoL impairment and a positive attitude towards implant treatment. Furthermore, patients can be informed that improvements in OHRQoL are expected to last for at least 5 years., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Comparative analysis of partial tuf sequences was evaluated for the identification and differentiation of lactobacilli. Comparison of the amino acid sequences allowed differentiation between species and also between the subspecies of Lactobacillus delbrueckii. The nucleotide sequence comparison allowed differentiation between other subspecies and between some strains. Lactobacilli from several collections and isolates from dairy samples were clearly identified by comparison of short tuf sequences with those of the type strains. In evaluating the taxonomy of the Lactobacillus casei-related taxa, different tuf amino acid signatures are in favour of a classification into three distinct species. The type strain designation for the L. casei species is discussed., (Copyright 2002 Federation of European Microbiological Societies)