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The purpose of the study is to investigate school counselors' support of youth experiencing homelessness going to college. Using survey methods, school counselors reported their knowledge, perceived competence, advocacy, and actions related to supporting students experiencing homelessness in their college preparation. The results suggested that training and the number of students experiencing homelessness on counselors' caseloads were significantly related to their knowledge and competence. Knowledge, competence, and advocacy all impacted the number of interventions utilized by participants. The implications of these results for school counselors and counselor educators are discussed.

Triboluminescence is a fascinating phenomenon that can engage students' interest in chemistry. However, safe, cost-effective, and accessible laboratory experiments featuring highly triboluminescent substances are limited. To bridge this gap, we present a simple, rapid, semimicro test tube preparation of the visually engaging manganese complex [MnBr[subscript 2](Ph[subscript 3]PO)[subscript 2]], conducted by high school students. During the laboratory session, students practice various laboratory skills, including handling semimicro quantities of substances, ascertaining the melting point, and recrystallizing the synthesized complex. They also investigate the complex's triboluminescent, fluorescent, and magnetic properties through straightforward and illustrative experiments. Furthermore, this laboratory activity has been successfully implemented in an undergraduate chemistry didactics course for chemistry educators. The experiment's simplicity, combined with the safety of starting materials and use of nontoxic solvents, makes it an optimal choice for a variety of educational environments, such as high school and university laboratory sessions, science clubs, and public science outreach activities.

Forestation can contribute to climate change mitigation. However, increasing frequency and intensity of climate extremes are posed to have profound impact on forests and consequently on the mitigation potential of forestation efforts. In this perspective, we critically assess forestation-reliant climate mitigation scenarios from five different integrated assessment models (IAMs) by showcasing the spatially explicit exposure of forests to fire weather and the simulated increase in global annual burned area. We provide a detailed description of the feedback from climate change to forest carbon uptake in IAMs. Few IAMs are currently accounting for feedback mechanisms like loss from fire disturbance. Consequently, many forestation areas proposed by IAM scenarios will be exposed to fire-promoting weather conditions and without costly prevention measures might be object to frequent burning. We conclude that the actual climate mitigation portfolio in IAM scenarios is subject to substantial uncertainty and that the risk of overly optimistic estimates of negative emission potential of forestation should be avoided. As a way forward we propose how to integrate more detailed climate information when modeling climate mitigation pathways heavily relying on forestation.

This qualitative study provides insight into the perceptions and experiences of local homeless liaisons supporting students experiencing homelessness who are preparing for and transitioning to college. Findings suggest that when it comes to preparing students experiencing homelessness for college, liaisons are very involved by addressing students' three main areas of need. These include their basic needs (i.e., physiological, safety, and academic) and social-emotional needs (i.e., love and belonging and esteem), as well as needs related to college-going (i.e., students' belief they can go to college) and expectations of college-going outcomes (i.e., students' belief they can graduate from college). Findings suggest that liaisons play a particularly important role in supporting the college-going of students experiencing homelessness and do so primarily by working collaboratively with a system of stakeholders.

Introduction: The majority of peppers in the US for fresh market and processing are handpicked, and harvesting can account for 20-50% of production costs. Innovation in mechanical harvesting would increase availability; lower the costs of local, healthy vegetable products; and perhaps improve food safety and expand markets. Most processed peppers require removal of pedicels (stem and calyx) from the fruit, but lack of an efficient mechanical process for this operation has hindered adoption of mechanical harvest. In this paper, we present characterization and advancements in breeding green chile peppers for mechanical harvesting. Specifically, we describe inheritance and expression of an easy-destemming trait derived from the landrace UCD-14 that facilitates machine harvest of green chiles. Methods: A torque gauge was used for measuring bending forces similar to those of a harvester and applied to two biparental populations segregating for destemming force and rate. Genotyping by sequencing was used to generate genetic maps for quantitative trait locus (QTL) analyses. Results: A major destemming QTL was found on chromosome 10 across populations and environments. Eight additional population and/or environment-specific QTL were also identified. Chromosome 10 QTL markers were used to help introgress the destemming trait into jalapeño-type peppers. Low destemming force lines combined with improvements in transplant production enabled mechanical harvest of destemmed fruit at a rate of 41% versus 2% with a commercial jalapeńo hybrid. Staining for the presence of lignin at the pedicel/fruit boundary indicated the presence of an abscission zone and homologs of genes known to affect organ abscission were found under several QTL, suggesting that the easy-destemming trait may be due to the presence and activation of a pedicel/fruit abscission zone. Conclusion: Presented here are tools to measure the easy-destemming trait, its physiological basis, possible molecular pathways, and expression of the trait in various genetic backgrounds. Mechanical harvest of destemmed mature green chile fruits was achieved by combining easy-destemming with transplant management.

Precise targeting of large transgenes to T cells using homology-directed repair has been transformative for adoptive cell therapies and T cell biology. Delivery of DNA templates via adeno-associated virus (AAV) has greatly improved knockin efficiencies, but the tropism of current AAV serotypes restricts their use to human T cells employed in immunodeficient mouse models. To enable targeted knockins in murine T cells, we evolved Ark313, a synthetic AAV that exhibits high transduction efficiency in murine T cells. We performed a genome-wide knockout screen and identified QA2 as an essential factor for Ark313 infection. We demonstrate that Ark313 can be used for nucleofection-free DNA delivery, CRISPR-Cas9-mediated knockouts, and targeted integration of large transgenes. Ark313 enables preclinical modeling of Trac-targeted CAR-T and transgenic TCR-T cells in immunocompetent models. Efficient gene targeting in murine T cells holds great potential for improved cell therapies and opens avenues in experimental T cell immunology.

Meeting ambitious climate targets will require deploying the full suite of mitigation options, including those that indirectly reduce greenhouse-gas (GHG) emissions. Healthy diets have sustainability co-benefits by directly reducing livestock emissions as well as indirectly reducing land use emissions. Increased crop productivity could indirectly avoid emissions by reducing cropland area. However, there is disagreement on the sustainability of proposed healthy U.S. diets and a lack of clarity on how long-term sustainability benefits may change in response to shifts in the livestock sector. Here, we explore the GHG emissions impacts of seven scenarios that vary U.S. crop yields and healthier diets in the U.S. and overseas. We also examine how impacts vary across assumptions of future ruminant livestock productivity and ruminant stocking density in the U.S. We employ two complementary land use models—the US FABLE Calculator, an agricultural and forestry sector accounting model with high agricultural commodity representation, and GLOBIOM, a spatially explicit partial equilibrium optimization model for global land use systems. Results suggest that healthier U.S. diets that follow the Dietary Guidelines for Americans reduce agricultural and land use greenhouse gas emissions by 25–57% (approx 120–310 MtCO2e/y) and pastureland area by 28–38%. The potential emissions and land sparing benefits of U.S. agricultural productivity growth are modest within the U.S. due to the increasing comparative advantage of U.S. crops. Our findings suggest that healthy U.S. diets can significantly contribute toward meeting U.S. long-term climate goals for the land use sectors.

The safety of drivers while driving is a key parameter in the car market. If we talk about the windshield`s influence and its composition on driver safety, the use of laminated glass with PVB (=polyvinyl butyral) is clearly key. Laminated glass created this way is called safety glass and fulfils the main function that, in the event of an accident, the glass will crack due to the stress created in the glass during its production, resulting in controlled shapes of glass fragments that are usually not sharp and will not cause serious injury to the driver or passengers. However, we can also discuss other features of the windshield, which will allow the driver to increase his or her driving comfort and thereby increase the driver`s concentration while driving. Such an example of a windshield is glass that has layers of coatings made of silver and various other oxides, such as zinc oxide, and reflects radiation in the infrared region of the spectrum. This allows the driver to optimise the conditions in the car, while not letting as much radiation out of the car in the winter or inside in the summer. Sunlight and heat adversely affect the condition of the car cabin and cause discomfort to both the driver and passengers. It also reduces the car`s air conditioning output. In addition, the sun`s UV and IR radiation negatively affect human health. Solar layers can reflect up to 99 % of infrared radiation. It all depends on their composition and the number of silver layers that primarily perform this function. Reflex UV and IR rays coming from the sun make the car cabin cooler and pleasant in summer. On the contrary, in winter it does not transmit IR radiation from the car, which again means an advantage for all the passengers in the car. At the same time, glasses composed in this way are environmentally friendly, as the amount of carbon dioxide emitted is reduced when they are used. The driver does not need to air-condition as much in the summer and, conversely, use the heating as much in the winter. The article discusses both the positive aspects of coated glasses for safety while driving, as well as the effects on the environment.

Abstract Seagrass meadows are important sinks for organic carbon and provide co-benefits. However, data on the organic carbon stock in seagrass sediments are scarce for many regions, particularly The Bahamas, which accounts for up to 40.7% of the documented global seagrass area, limiting formulation of blue carbon strategies. Here, we sampled 10 seagrass meadows across an extensive island chain in The Bahamas. We estimate that Bahamas seagrass meadows store 0.42–0.59 Pg organic carbon in the top-meter sediments with an accumulation rate of 2.1–2.9 Tg annually, representing a substantial global blue carbon hotspot. Autochthonous organic carbon in sediments decreased from ~1980 onwards, with concomitant increases in cyanobacterial and mangrove contributions, suggesting disturbance of seagrass ecosystems, likely caused by tourism and maritime traffic activities. This study provides seagrass blue carbon data from a vast, understudied region and contributes to improving climate action for The Bahamas and the Greater Caribbean region.

This article describes the experience of participants and facilitators engaging in a psychoeducational group counseling intervention designed to enhance college readiness among Latine first-generation college-going students--those for whom neither parent completed college (National Center for Education Statistics, 2017). The group was designed to increase students' understanding of how their various identities impacted their college preparatory experience and willingness to seek help. Findings suggest that participants recognized the importance of their identities and the way they influenced their help-seeking behaviors. Participants identified similarities among their experiences related to college going while also valuing learning from each other's differences. Last, participants felt a sense of uncertainty and angst about going to college. We also share facilitator experiences.

Students with disabilities are at greater risk for depression, substance use, bullying, and fewer friendships, largely due to negative attitudes and misperceptions from their nondisabled peers. School counselors are particularly important stakeholders in improving the experiences of students with disabilities. This qualitative study uses the American Counseling Association (ACA) Advocacy Competencies as a lens to understand how six school counselors raise disability awareness within the context of social justice advocacy. Results indicate that participants primarily focused on ways to advocate alongside or on behalf of students with disabilities at an individual level (client/student domain). Additional findings highlight the need for increased disability-related training within counselor preparation programs and the importance of including disability issues within the frame of multicultural competence. The results of this study fill a gap in the literature and lead to a deeper understanding of how school counselors are presently engaging in disability programs in their schools. Additionally, findings from this research directly aid in the construction of coursework and related experiences that would enhance the preparation of pre-service school counselors.

Summary: Adeno-associated virus (AAV) is a member of the genus Dependoparvovirus, which infects a wide range of vertebrate species. Here, we observe that, unlike most primate AAV isolates, avian AAV is transcriptionally silenced in human cells. By swapping the VP1 N terminus from primate AAVs (e.g., AAV8) onto non-mammalian isolates (e.g., avian AAV), we identify a minimal component of the AAV capsid that controls viral transcription and unlocks robust transduction in both human cells and mouse tissue. This effect is accompanied by increased AAV genome chromatin accessibility and altered histone methylation. Proximity ligation analysis reveals that host factors are selectively recruited by the VP1 N terminus of AAV8 but not avian AAV. Notably, these include AAV essential factors implicated in the nuclear factor κB pathway, chromatin condensation, and histone methylation. We postulate that the AAV capsid has evolved mechanisms to recruit host factors to its genome, allowing transcriptional activation in a species-specific manner.

In the search for alternatives to the commonly used methods of the pre-treatment of surfaces, plasma discharge applications are appearing more and more. In the case of the automotive industry, where the attention is focused on finding methods that are more environmentally friendly, more economically advantageous and process-friendly, plasma is a suitable option. In the case of gluing additional parts with polyurethane glue to the car glass, the use of plasma activation or cleaning of the joined materials improves the adhesion properties. Through this process, it is possible to achieve an increase in the force required to break the joint, as well as an increase in the proportion of the desired cohesive failure of the adhesive. A process including a point nozzle plasma used on the glass surface and a rotating plasma nozzle to activate the surface of the holder made of polyamide 6 with a glass fibre content of 30% was found to be particularly suitable. The effect of plasma was also investigated using a surface tension test using test inks, observing the materials using an optical microscope, and the cleaning efficiency was analysed using infrared spectroscopy.

During the float glass process in a float bath, tin diffuses into the surface of the glass. The final product of the float process has two sides - one tin side and the other air side of the glass. In the next step, a layer of material is applied to the air side of the glass, which most often has an anti-reflective function, consisting of several layers, where the primary function is performed by a layer of silver. Such glass is used in the construction or automotive industry, for example. A problem arises, however, if the float glass is processed by another process where the glass is heated. When the glass is reheated, defects may appear on the tin side of the float blank due to the diffusion of ions present on the tin side of the float glass to its surface. The article deals with the diffusion of silver on the tin side of float glass in the laboratory and the subsequent analysis of the defect. The goal is to discuss the mechanism of this phenomenon and the possible methods to prevent the defect from occurring.

Abstract Oceans are crucial to human survival, providing natural resources and most of the global oxygen supply, and are responsible for a large portion of worldwide economic development. Although it is widely considered a silent world, the sea is filled with natural sounds generated by marine life and geological processes. Man-made underwater sounds, such as active sonars, maritime traffic, and offshore oil and mineral exploration, have significantly affected underwater soundscapes and species. In this work, we report on a joint optical fiber-based communication and sensing technology aiming to reduce noise pollution in the sea while providing connectivity simultaneously with a variety of underwater applications. The designed multifunctional fiber-based system enables two-way data transfer, monitoring marine life and ship movement near the deployed fiber at the sea bottom and sensing temperature. The deployed fiber is equally harnessed to transfer energy that the internet of underwater things (IoUTs) devices can harvest. The reported approach significantly reduces the costs and effects of monitoring marine ecosystems while ensuring data transfer and ocean monitoring applications and providing continuous power for submerged IoUT devices.

Objectives To compare industry payment patterns among US psychiatrists and psychiatric advanced practice clinicians (APCs) and determine how scope of practice laws has influenced these patterns.Design Cross-sectional study.Setting This study used the publicly available US Centers for Medicare and Medicaid Services Sunshine Act Open Payment database and the National Plan and Provider Enumeration System (NPPES) database for the year 2021.Participants All psychiatrists and psychiatric APCs (subdivided into nurse practitioners (NPs) and clinical nurse specialists (CNSs)) included in either database.Primary and secondary outcome measures Number and percentage of clinicians receiving industry payments and value of payments received. Total payments and number of transactions by type of payment, payment source and clinician type were also evaluated.Results A total of 85 053 psychiatric clinicians (61 011 psychiatrists (71.7%), 21 895 NPs (25.7%), 2147 CNSs (2.5%)) were reviewed; 16 240 (26.6%) psychiatrists received non-research payment from industry, compared with 10 802 (49.3%) NPs and 231 (10.7%) CNSs (p United States Dollars (US) $) 100 (33.9% vs 14.6%; IRR, 2.14 (2.08 to 2.20); p US$ 1000 (5.3% vs 4.1%; IRR, 1.29 (1.20 to 1.38); p US$ 10 000 (0.4% vs 1.0%; IRR, 0.39 (0.31 to 0.49); p

The association of subjective mental health-related quality of life (MHRQOL) and treatment use among people experiencing common substance use disorders (SUDs) is not known. Furthermore, the association of a given substance's legal status with treatment use has not been studied. This work aims determine the association of MHRQOL with SUD treatment use, and how substance legal status modulates this relationship. Our analysis used nationally-representative data from the NESARC-III database of those experiencing past-year SUDs (n = 5,808) to compare rates of treatment use and its correlates among three groups: those with illicit substance use disorders (ISUDs); those with partially legal substance use disorders, i.e., cannabis use disorder (CUD); and those with fully legal substance use disorders, i.e., alcohol use disorder (AUD). Survey-weighted multiple regression analysis was used to assess the association of MHRQOL with likelihood of treatment use among these three groups, both unadjusted and adjusted for sociodemographic, behavioral, and diagnostic factors. Adults with past-year ISUDs were significantly more likely to use treatment than those with CUD and AUD. Among those with ISUDs, MHRQOL had no significant association with likelihood of treatment use. Those with past-year CUD saw significant negative association of MHRQOL with treatment use in unadjusted analysis, but not after controlling for diagnostic and other behavioral health factors. Those with past-year AUD had significant negative association of MHRQOL with treatment use in both unadjusted and adjusted analysis. If legalization and decriminalization continue, there may be a greater need for effective public education and harm reduction services to address this changing SUD landscape.

Abstract The issues surrounding 'Reduced Emissions from Deforestation and Forest Degradation' (REDD) have become a major component of continuing negotiations under the United Nations Framework Convention on Climate Change (UNFCCC). This paper aims to address two key requirements of any potential REDD mechanism: first, the generation of measurable, reportable and verifiable (MRV) REDD credits; and secondly, the sustainable and efficient provision of emission reductions under a robust financing regime. To ensure the supply of MRV credits, we advocate the establishment of an 'International Emission Reference Scenario Coordination Centre' (IERSCC). The IERSCC would act as a global clearing house for harmonized data to be used in implementing reference level methodologies. It would be tasked with the collection, reporting and subsequent processing of earth observation, deforestation- and degradation driver information in a globally consistent manner. The IERSCC would also assist, coordinate and supervise the computation of national reference scenarios according to rules negotiated under the UNFCCC. To overcome the threats of "market flooding" on the one hand and insufficient economic incentives for REDD on the other hand, we suggest an 'International Investment Reserve' (IIR) as REDD financing framework. In order to distribute the resources of the IIR we propose adopting an auctioning mechanism. Auctioning not only reveals the true emission reduction costs, but might also allow for incentivizing the protection of biodiversity and socio-economic values. The introduced concepts will be vital to ensure robustness, environmental integrity and economic efficiency of the future REDD mechanism.

Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.

This article describes the unique college and career preparation challenges faced by students experiencing homelessness (SEH), framed using a Social Cognitive Career Theory (SCCT) lens. The experience of homelessness presents barriers for secondary students, which can impact their college self-efficacy, outcome expectations of attending and succeeding in college, and goal setting towards college. In this conceptual paper, background on homelessness and research related to the college planning process of SEH is provided, as well as implications for school and career counselors, as well as other educators.

Abstract Recombinant adeno-associated viral (AAV) vectors are a promising gene delivery platform, but ongoing clinical trials continue to highlight a relatively narrow therapeutic window. Effective clinical translation is confounded, at least in part, by differences in AAV biology across animal species. Here, we tackle this challenge by sequentially evolving AAV capsid libraries in mice, pigs and macaques. We discover a highly potent, cross-species compatible variant (AAV.cc47) that shows improved attributes benchmarked against AAV serotype 9 as evidenced by robust reporter and therapeutic gene expression, Cre recombination and CRISPR genome editing in normal and diseased mouse models. Enhanced transduction efficiency of AAV.cc47 vectors is further corroborated in macaques and pigs, providing a strong rationale for potential clinical translation into human gene therapies. We envision that ccAAV vectors may not only improve predictive modeling in preclinical studies, but also clinical translatability by broadening the therapeutic window of AAV based gene therapies.

IntroductionCoeliac disease is a lifelong immune-mediated enteropathy manifested as gluten intolerance in individuals carrying specific human leukocyte antigen (HLA) molecules. Other factors than genetics and gluten intake, however, may play a role in triggering the disease. The gut internal environment is thought to be one of these potential contributing factors, and it can be influenced throughout life.MethodsWe examine the impact of Lactiplantibacillus plantarum HEAL9 and Lacticaseibacillus paracasei 8700:2 supplementation on the faecal metabolome in genetically predisposed children having tissue transglutaminase autoantibodies, i.e., coeliac disease autoimmunity. Probiotic strains were selected based on their beneficial properties, including mucosal permeability and immune modulation effects. The intervention group (n = 40) and control group (n = 38) took the probiotics or placebo daily for 6 months in a double-blinded randomised trial. Faecal samples were collected at baseline and after 3 and 6 months and analysed using the 1H NMR for metabolome. The incorporation of 16S rRNA sequencing as a supportive dataset complemented the analysis of the metabolome data.ResultsDuring the 6 months of intervention, the stool concentrations of 4-hydroxyphenylacetate increased in the intervention group as compared to controls, whereas concentrations of threonine, valine, leucine, isoleucine, methionine, phenylalanine, aspartate, and fumarate decreased. Additionally, a noteworthy effect on the glycine, serine, and threonine metabolic pathway has been observed.ConclusionThe findings suggest a modest yet significant impact of the probiotics on the faecal metabolome, primarily influencing proteolytic processes in the gut.Clinical trial registrationClinicalTrials.gov, NCT03176095.

Seagrass meadows store significant amounts of carbonate (CaCO _3 ) in sediment, contributing to coastal protection but potentially offsetting their effectiveness as carbon sinks. Understanding the accumulation of CaCO _3 and its balance with organic carbon (C _org ) in seagrass ecosystems is crucial for developing seagrass-based blue carbon strategies for climate change mitigation. However, CaCO _3 accumulation in seagrass meadows varies significantly across geographic regions, with notable data gaps in the Caribbean and Central America. Here, we sampled 10 seagrass meadows across an extensive island chain in The Bahamas, part of the largest seagrass ecosystem and one of the largest CaCO _3 banks globally, to evaluate CaCO _3 stock, accumulation rate, and its balance with C _org sequestration. Seagrass meadows in The Bahamas store 6405–8847 Tg of inorganic carbon (C _inorg ) in the upper meter sediment, with an annual accumulation rate of 38.3–52.9 Tg of C _inorg , highlighting these meadows as hotspots for CaCO _3 burial. CaCO _3 contributes 67 ± 8% (mean ± standard error) of the sediment accumulation, indicating its important role in seabed elevation. Sediment C _inorg showed no significant relationship with C _org , with an average C _org : C _inorg ratio of 0.069 ± 0.002, ∼ 10 times lower than the threshold (C _org : C _inorg ratio of about 0.63) at which seagrass ecosystem transition from CO _2 sources to sinks. However, the available air–sea gas flux measurement was only 1/5 of the calculated CO _2 emission expected from calcification, suggesting that part of the accumulated CaCO _3 is supported by allochthonous inputs. Furthermore, no perceivable relationship between seagrass density and either CaCO _3 stock or accumulation rate was observed, indicating that seagrass may play a limited role in supporting CaCO _3 production. Further studies on water chemistry, calcification rate, air–sea CO _2 flux, and comparison between seagrass and unvegetated habitats are required to elucidate the carbon budget of this globally significant ecosystem.

Rapidly increasing populations coupled with increased food demand requires either an expansion of agricultural land or sufficient production gains from current resources. However, in a changing world, reduced water availability might undermine improvements in crop and grass productivity and may disproportionately affect different parts of the world. Using multi-model studies, the potential trends, risks and uncertainties to land use and land availability that may arise from reductions in water availability are examined here. In addition, the impacts of different policy interventions on pressures from emerging risks are examined. Results indicate that globally, approximately 11% and 10% of current crop- and grass-lands could be vulnerable to reduction in water availability and may lose some productive capacity, with Africa and the Middle East, China, Europe and Asia particularly at risk. While uncertainties remain, reduction in agricultural land area associated with dietary changes (reduction of food waste and decreased meat consumption) offers the greatest buffer against land loss and food insecurity.

ABSTRACT: Detection of adulteration of small ruminant milk is very important for health and commercial reasons. New analytical and cost-effective methods need to be developed to detect new adulteration practices. In this work, we aimed to explore the ability of the MALDI-TOF mass spectrometry to detect bovine milk in caprine and ovine milk using samples from 18 dairy farms. Different levels of adulteration (0.5, 1, 5, 10, 20, 40, 60, and 80%) were analyzed during the lactation period of goat and sheep (in May, from 60 to 90 d in milk, and in August, from 150 to 180 d in milk). Two different ranges of peptide-protein spectra (500–4,000 Da; 4–20 kDa) were used to establish a calibration model for predicting the concentration of adulterant using partial least squares and generalized linear model with lasso regularization. The low molecular weight part of the spectra together with the generalized linear model with lasso regularization regression model appeared to have greater potential for our aim of detection of adulteration of small ruminants' milk. The subsequent prediction model was able to predict the concentration of bovine milk in caprine milk with a root mean square error of 11.4 and 17.0% in ovine milk. The results offer compelling evidence that MALDI-TOF can detect the adulteration of small ruminants' milk. However, the method is severely limited by (1) the complexity of the milk proteome resulting from the adulteration technique, (2) the potential degradation of thermolabile proteins, and (3) the genetic variability of tested samples. Additionally, the root mean square error of prediction based only on one individual sample adulteration series can drop down to 6.34% for quantification of adulterated caprine milk and 6.28% for adulterated ovine milk for the full set of concentrations or down to 2.33 and 4.00%, respectively, if we restrict only to low concentrations of adulteration (0, 0.5, 1, 5, 10%).

Introduction: The majority of peppers in the US for fresh market and processing are handpicked, and harvesting can account for 20–50% of production costs. Innovation in mechanical harvesting would increase availability; lower the costs of local, healthy vegetable products; and perhaps improve food safety and expand markets. Most processed peppers require removal of pedicels (stem and calyx) from the fruit, but lack of an efficient mechanical process for this operation has hindered adoption of mechanical harvest. In this paper, we present characterization and advancements in breeding green chile peppers for mechanical harvesting. Specifically, we describe inheritance and expression of an easy-destemming trait derived from the landrace UCD-14 that facilitates machine harvest of green chiles.Methods: A torque gauge was used for measuring bending forces similar to those of a harvester and applied to two biparental populations segregating for destemming force and rate. Genotyping by sequencing was used to generate genetic maps for quantitative trait locus (QTL) analyses.Results: A major destemming QTL was found on chromosome 10 across populations and environments. Eight additional population and/or environment-specific QTL were also identified. Chromosome 10 QTL markers were used to help introgress the destemming trait into jalapeño-type peppers. Low destemming force lines combined with improvements in transplant production enabled mechanical harvest of destemmed fruit at a rate of 41% versus 2% with a commercial jalapeńo hybrid. Staining for the presence of lignin at the pedicel/fruit boundary indicated the presence of an abscission zone and homologs of genes known to affect organ abscission were found under several QTL, suggesting that the easy-destemming trait may be due to the presence and activation of a pedicel/fruit abscission zone.Conclusion: Presented here are tools to measure the easy-destemming trait, its physiological basis, possible molecular pathways, and expression of the trait in various genetic backgrounds. Mechanical harvest of destemmed mature green chile fruits was achieved by combining easy-destemming with transplant management.

This qualitative study focused on examining the roles and experiences of high school counselors supporting students experiencing homelessness in their college preparation. To be included in the study, participants needed to have experience working with high school students experiencing homelessness. Findings indicated that the participants supported students experiencing homelessness by (a) enhancing access and exposure to college, (b) providing hands-on and individualized college-going support, and (c) building partnerships with universities to support college going.

Background: Self-inflicted gunshot wounds (SIGSWs) produce devastating facial defects that are challenging to reconstruct, but are rarely reported in large cohorts in the literature. This study sought to characterize these injuries, and identify parameters influencing complications and outcomes among survivors following facial reconstruction. Methods: A retrospective cohort study was performed identifying 22 patients with SIGSWs to the face reconstructed at our center from 2009 to 2019. Charts were reviewed for patient, injury, and reconstructive details and course. Outcomes were statistically compared to various parameters. Results: The most common firearm, orientation, and injured structure were the handgun (40.9%), submental (59.1%), and mandible (68.2%), respectively. Patients averaged a 21.7-day length of stay (LOS), 17.4 h to debridement, 2.6 days to bony fixation, 5.4 reconstructive surgeries, and 7 (31.8%) patients received at least one free flap. Fifteen (68.2%) patients had at least one major complication, although functional outcomes were ultimately relatively good overall. Notable outcome associations included submental orientation with a longer LOS (p = 0.027), external fixation with a longer LOS (p = 0.014), financial stressors with a shorter LOS (p = 0.031), and severe soft tissue injury with an increased total number of reconstructive surgeries (p = 0.039) and incomplete reconstruction (p = 0.031). There were no cases of suicidal recidivism. Conclusions: Reconstruction following facial SIGSW is challenging for both patient and surgeon, and carries a high rate of complications. However, patients can regain substantial function following reconstruction and the achievement of satisfactory outcomes.

Through use of an interpretive phenomenological inquiry, this study examined the lived experiences of first-generation college students persisting at a mid-sized, private, predominantly White institution. Themes that emerged across participants included a sense of "otherness" according to students' race, ethnicity, and first-generation and socioeconomic statuses. Motivations and strengths that enabled students to persist in school, despite facing multiple obstacles, are described. Implications for students and helping professionals are included.

We applied a qualitative inquiry to explore the experiences of African American youth engaging in a group intervention at an urban high school. Group topics were tailored to the needs of prospective first-generation college students to enhance youth social and academic capitol and identity strengthening. Experiences included changed perspectives and increased knowledge of the college-going experience that, in turn, increased participant sense of efficacy in completing college. We discuss implications for school counselors.

This article describes an eight-session psychoeducational small-group intervention with two groups of African-American youth in an urban, east coast high school setting. Sessions were designed to enhance the self-efficacy and outcomes expectations related to attending college for first-generation college goers in the twelfth grade. The group session topics are described, and outcomes are assessed. Facilitator challenges unique to the group setting are included.