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153 results on '"Hay, Fiona R."'

2. New technologies to improve the ex situ conservation of plant genetic resources

Author

Hay, Fiona R. and Sershen, Dr

Subjects
cryopreservation, genebank management, seed storage behaviour, viability monitoring, thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PST Botany and plant sciences, thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVK Agronomy and crop production, thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVF Sustainable agriculture, thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming::TVB Agricultural science
Abstract

Access to plant genetic resources is fundamental to the development of more resilient and nutritious crops. The efficient and effective conservation of plant genetic resources is therefore key to ensuring global food security. There are more than 1750 genebanks around the world, storing various types of plant germplasm including tissue cultures, seeds, embryos and pollen. The method of conservation depends on the seed storage behaviour and propagation requirements of the species. The germplasm of most major crops is conserved as seeds under ‘conventional’ genebank storage conditions, because the seeds tolerate drying to low moisture content and survive storage, perhaps for many decades, at low temperature. Other species are typically conserved as tissue cultures and/or as cryopreserved explants. New technologies for the routine conservation of seeds in genebanks includes evidence-based refining of procedures and the introduction of automation. Similarly, in vitro culture and cryopreservation techniques continue to be developed and improved.

Published
2021
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9. Seed viability constants and the decline in germination rates for radish: a combined viability equation and population-based threshold model analysis

Author

Coronado, Nadine B., primary, Gentallan Jr., Renerio P., additional, Hay, Fiona R., additional, Madayag, Roselle E., additional, Timog, Emmanuel Bonifacio S., additional, Quiñones, Kristine Joyce O., additional, Bartolome, Michael Cedric B., additional, Endonela, Leah E., additional, Altoveros, Nestor C., additional, and Borromeo, Teresita H., additional

Published
2023
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14. Cereals

Author

Hay, Fiona R., Hamilton, N. Ruaraidh Sackville, Furman, Bonnie J., Upadhyaya, Hari D., Reddy, K. N., Singh, S. K., Normah, M.N., editor, Chin, H.F., editor, and Reed, Barbara M., editor

Published
2013
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16. Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions.

Author

Prasad C. T., Manjunath, Kodde, Jan, Angenent, Gerco C., Hay, Fiona R., McNally, Kenneth L., and Groot, Steven P. C.

Subjects
REGULATOR genes, RICE seeds, GENOME-wide association studies, SINGLE nucleotide polymorphisms, RICE, GENETIC variation
Abstract

Seed deterioration during storage results in poor germination, reduced vigour, and non‐uniform seedling emergence. The aging rate depends on storage conditions and genetic factors. This study aims to identify these genetic factors determining the longevity of rice (Oryza sativa L.) seeds stored under experimental aging conditions mimicking long‐term dry storage. Genetic variation for tolerance to aging was studied in 300 Indica rice accessions by storing dry seeds under an elevated partial pressure of oxygen (EPPO) condition. A genome‐wide association analysis identified 11 unique genomic regions for all measured germination parameters after aging, differing from those previously identified in rice under humid experimental aging conditions. The significant single nucleotide polymorphism in the most prominent region was located within the Rc gene, encoding a basic helix‐loop‐helix transcription factor. Storage experiments using near‐isogenic rice lines (SD7‐1D (Rc) and SD7‐1d (rc) with the same allelic variation confirmed the role of the wildtype Rc gene, providing stronger tolerance to dry EPPO aging. In the seed pericarp, a functional Rc gene results in accumulation of proanthocyanidins, an important sub‐class of flavonoids having strong antioxidant activity, which may explain the variation in tolerance to dry EPPO aging. Summary statement: The wildtype Rc gene provides protection against rice seed aging under dry storage conditions, as was identified by a genome‐wide association study using 300 Indica accessions. Its role was validated using near‐isogenic lines with a non‐functional gene or containing the functional Rc gene introgressed from wild rice. [ABSTRACT FROM AUTHOR]

Published
2023
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25. Experimental rice seed aging under elevated oxygen pressure: Methodology and mechanism.

Author

C. T., Manjunath Prasad, Kodde, Jan, Angenent, Gerco C., de Vos, Ric C. H., Diez-Simon, Carmen, Mumm, Roland, Hay, Fiona R., Siricharoen, Sasiwimon, Yadava, Devendra K., and Groot, Steven P. C.

Subjects
SEED storage, AGING, RICE seeds, RICE storage, RICE, PARTIAL pressure, HUMIDITY, SEED viability, METABOLOMICS
Abstract

Seed aging during storage results in loss of vigor and germination ability due to the accumulation of damage by oxidation reactions. Experimental aging tests, for instance to study genetic variation, aim to mimic natural aging in a shorter timeframe. As the oxidation rate is increased by elevating the temperature, moisture, and oxygen levels, this study aimed to (1) investigate the effect of experimental rice seed aging by an elevated partial pressure of oxygen (EPPO), (2) elucidate the mechanism of dry-EPPO aging and (3) compare aging under dry-EPPO conditions to aging under traditional moist-controlled deterioration (CD) conditions and to long-term ambient storage. Dry seeds from 20 diverse rice accessions were experimentally aged under EPPO (200 times higher oxygen levels), at 50% relative humidity (RH), along with storage under highpressure nitrogen gas and ambient conditions as controls. While no decline in germination was observed with ambient storage, there was significant aging of the rice seeds under EPPO storage, with considerable variation in the aging rate among the accessions, with an average decline toward 50% survival obtained after around 21 days in EPPO storage and total loss of germination after 56 days. Storage under high-pressure nitrogen gas resulted in a small but significant decline, by an average of 5% germination after 56 days. In a second experiment, seven rice seed lots were stored under EPPO as compared to a moist-CD test and two different long-term ambient storage conditions, i.e., conditioned warehouse seed storage (CWSS) and traditional rice seed storage (TRSS). Untargeted metabolomics (with identification of lipid and volatile compounds profiles) showed a relatively high increase in levels of oxidized lipids and related volatiles under all four storage conditions. These compounds had a high negative correlation with seed viability, indicating oxidation as a main deteriorating process during seed aging. Correlation analysis indicated that EPPO storage at 50% RH is more related to aging under TRSS at 60% and CD-aging at 75% ERH rather than CWSS at 40% ERH. In conclusion, aging rice seeds under EPPO conditions is a suitable experimental aging method for analyzing variation among seed lots or genotypes for longevity under storage. [ABSTRACT FROM AUTHOR]

Published
2022
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30. Cereals

Author

Hay, Fiona R., primary, Hamilton, N. Ruaraidh Sackville, additional, Furman, Bonnie J., additional, Upadhyaya, Hari D., additional, Reddy, K. N., additional, and Singh, S. K., additional

Published
2012
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38. Variation in seed longevity among diverse Indica rice varieties

Author

Lee, Jae-Sung, primary, Velasco-Punzalan, Marlina, additional, Pacleb, Myrish, additional, Valdez, Rocel, additional, Kretzschmar, Tobias, additional, McNally, Kenneth L, additional, Ismail, Abdel M, additional, Cruz, Pompe C Sta, additional, Sackville Hamilton, N Ruaraidh, additional, and Hay, Fiona R, additional

Published
2019
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49. Resilience of rice (Oryza spp.) pollen germination and tube growth to temperature stress

Author

Coast, Onoriode, Murdoch, Alistair J., Ellis, Richard H., Hay, Fiona R., and Jagadish, Krishna S.V.

Subjects
GE, S1
Abstract

Resilience of rice cropping systems to potential global climate change will partly depend on the temperature tolerance of pollen germination (PG) and tube growth (PTG). Pollen germination of high temperature-susceptible Oryza glaberrima Steud. (cv. CG14) and Oryza sativa L. ssp. indica (cv. IR64) and high temperature-tolerant O. sativa ssp. aus (cv. N22), was assessed on a 5.6–45.4 °C temperature gradient system. Mean maximum PG was 85% at 27 °C with 1488 µm PTG at 25 °C. The hypothesis that in each pollen grain, the minimum temperature requirements (Tn) and maximum temperature limits (Tx) for germination operate independently was accepted by comparing multiplicative and subtractive probability models. The maximum temperature limit for PG in 50% of grains (Tx(50)) was the lowest (29.8 °C) in IR64 compared with CG14 (34.3 °C) and N22 (35.6 °C). Standard deviation (sx) of Tx was also low in IR64 (2.3 °C) suggesting that the mechanism of IR64’s susceptibility to high temperatures may relate to PG. Optimum germination temperatures and thermal times for 1 mm PTG were not linked to tolerating high temperatures at anthesis. However, the parameters Tx(50) and sx in the germination model define new pragmatic criteria for successful and resilient PG, preferable to the more traditional cardinal (maximum and minimum) temperatures.

Published
2015

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