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168 results on '"Hayward, Daniel"'

1. Use of a non-endoscopic immunocytological device (Cytosponge™) for post chemoradiotherapy surveillance in patients with oesophageal cancer in the UK (CYTOFLOC): A multicentre feasibility study

Author

Jones, Christopher M., O'Connor, Heather, O'Donovan, Maria, Hayward, Daniel, Blasko, Adrienn, Harman, Ruth, Malhotra, Shalini, Debiram-Beecham, Irene, Alias, Bincy, Bailey, Adam, Bateman, Andrew, Crosby, Tom D.L., Falk, Stephen, Gollins, Simon, Hawkins, Maria A., Kadri, Sudarshan, Levy, Stephanie, Radhakrishna, Ganesh, Roy, Rajarshi, Sripadam, Raj, Fitzgerald, Rebecca C., and Mukherjee, Somnath

Published
2022
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2. The spindle assembly pathways of the Drosophila syncytial embryo

Author

Hayward, Daniel and Wakefield, James

Subjects
500
Abstract

The mitotic spindle is a key cellular structure responsible for aligning and separating chromosomes during mitosis. It is vital that this process is performed faithfully, as errors can result in disease causing genome instability. The mitotic spindle is primarily composed of an organised mass of dynamic microtubules that form a bipolar structure. Different cells have been shown to employ varying molecular pathways to generate these microtubules; however how these multiple pathways coexist and coordinate in a single cell type is poorly understood. Using the Drosophila syncytial embryo as a model cell type, the work presented in this thesis provides evidence of multiple spindle assembly routes employed within this system. Development of a technique to disrupt the temporal controls on microtubule generation combined with high speed confocal microscopy has allowed for the direct visualisation of a chromatin mediated microtubule generation pathway, a process shown to require Ran-GTP, D-HURP and the Augmin complex, but surprisingly not by a Drosophila homologue of the vertebrate spindle assembly factor TPX2, also identified here. Disruption of centrosome assembly has also revealed the importance of Augmin in generating microtubules for spindle assembly from multiple acentrosomal microtubule organising centres. Additionally, work presented here demonstrates that spindle assembly pathways can increase their microtubule generating capacity when the activity of another pathway has been perturbed, showing a degree of coordination. This thesis demonstrates the remarkable robustness with which the mitotic spindle can assemble while providing an insight into the mechanisms behind this flexibility.

Published
2014

4. Mom Liked Me Better, and She Will Prove It Pre-Mortem Validation - Permanent Solution or Fad?

Author

Sneeringer, Michael, Arentz, Elizabeth A., Hayward, Daniel F., Ounanian, Melineh S., and Alaimo, Marve Ann M.

Subjects
Wills -- Laws, regulations and rules, Undue influence -- Laws, regulations and rules, Estate planning -- Laws, regulations and rules -- Methods, Probate law -- Evaluation -- Evidence, Witnesses -- Laws, regulations and rules, Government regulation
Abstract

In its March/April 2013 issue, Probate & Property readers were provided with an overview of pre-mortem validation. In Before the Party's Over--The Arguments for and Against Pre-Death Will Contests, authors [...]

Published
2021

5. Validating the Nima-related kinase Nek2 as a novel chemotherapeutic target

Author

Hayward, Daniel

Subjects
616.99
Abstract

Mitotic division in human cells prompts a dramatic rearrangement of cellular architecture chromosome condensation, nuclear envelope breakdown and bipolar spindle formation facilitates segregation of sister chromatids into daughter cells. Nek2 is a cell cycle-regulated serine/threonine kinase which is implicated in mitotic progression and establishment of a bipolar spindle. Overexpression of Nek2A in cultured human cells has resulted in an increased incidence of spindle abnormalities and aneuploidy, suggestive of defects in chromosome segregation. Currently there is considerable interest in determining whether centrosomal protein kinases that promote mitotic progression, such as Cdk1, Aurora-A, Plk1 and Nek2, are altered in tumour cells, are tumorigenic when upregulated and hence are viable chemotherapeutic targets. In this thesis I demonstrate that levels of Nek2 protein are upregulated in cell lines derived from leukaemia, breast and ovarian cancers. Importantly, I extended this analysis to primary human tumours and found Nek2 protein expression was increased in sixteen of twenty breast tumours studied, the first report of increased Nek2 protein in human neoplastic disease. I next established that the ablation of Nek2 by siRNA in cultured cervical carcinoma cells induced apoptosis. Lastly I present a series of small molecules that inhibit Nek2A in vitro and show the development of two cell-based assays of Nek2A activity, centrosomal disjunction and displacement of the inter-centrosomal component rootletin. Using stable cell lines inducibly expressing Nek2A I demonstrate ablation of Nek2 dependent phenotypes following treatment with a small molecule inhibitor. The ability to inhibit Nek2 in cells coupled with the observation that Nek2 depletion reduces the viability of cultured cells means Nek2 should now be considered a candidate for therapeutic intervention.

Published
2007

6. Organic Electronic Platform for Real‐Time Phenotypic Screening of Extracellular‐Vesicle‐Driven Breast Cancer Metastasis

Author

Traberg, Walther C., primary, Uribe, Johana, additional, Druet, Victor, additional, Hama, Adel, additional, Moysidou, Chrysanthi‐Maria, additional, Huerta, Miriam, additional, McCoy, Reece, additional, Hayward, Daniel, additional, Savva, Achilleas, additional, Genovese, Amaury M. R., additional, Pavagada, Suraj, additional, Lu, Zixuan, additional, Koklu, Anil, additional, Pappa, Anna‐Maria, additional, Fitzgerald, Rebecca, additional, Inal, Sahika, additional, Daniel, Susan, additional, and Owens, Róisín M., additional

Published
2023
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7. Organic Electronic Platform for Real-Time Phenotypic Screening of Extracellular-Vesicle-Driven Breast Cancer Metastasis

Author

Traberg, Walther C, Uribe, Johana, Druet, Victor, Hama, Adel, Moysidou, Chrysanthi-Maria, Huerta, Miriam, McCoy, Reece, Hayward, Daniel, Savva, Achilleas, Genovese, Amaury MR, Pavagada, Suraj, Lu, Zixuan, Koklu, Anil, Pappa, Anna-Maria, Fitzgerald, Rebecca, Inal, Sahika, Daniel, Susan, Owens, Róisín M, Traberg, Walther C [0000-0003-2673-2253], Owens, Róisín M [0000-0001-7856-2108], and Apollo - University of Cambridge Repository

Subjects
organic electronics, breast cancer metastasis, drug screening, exosomes, extracellular vesicles
Abstract

Tumor-derived extracellular vesicles (TEVs) induce the epithelial-to-mesenchymal transition (EMT) in nonmalignant cells to promote invasion and cancer metastasis, representing a novel therapeutic target in a field severely lacking in efficacious antimetastasis treatments. However, scalable technologies that allow continuous, multiparametric monitoring for identifying metastasis inhibitors are absent. Here, the development of a functional phenotypic screening platform based on organic electrochemical transistors (OECTs) for real-time, noninvasive monitoring of TEV-induced EMT and screening of antimetastatic drugs is reported. TEVs derived from the triple-negative breast cancer cell line MDA-MB-231 induce EMT in nonmalignant breast epithelial cells (MCF10A) over a nine-day period, recapitulating a model of invasive ductal carcinoma metastasis. Immunoblot analysis and immunofluorescence imaging confirm the EMT status of TEV-treated cells, while dual optical and electrical readouts of cell phenotype are obtained using OECTs. Further, heparin, a competitive inhibitor of cell surface receptors, is identified as an effective blocker of TEV-induced EMT. Together, these results demonstrate the utility of the platform for TEV-targeted drug discovery, allowing for facile modeling of the transient drug response using electrical measurements, and provide proof of concept that inhibitors of TEV function have potential as antimetastatic drug candidates.

Published
2023

16. Contributors

Author

Bailey, Ann G., primary, Balzer, Jeffrey R., additional, Baum, Victor C., additional, Beebe, David S., additional, Beers, Sue R., additional, Belani, Kumar G., additional, Bissonette, Bruno, additional, Blasiole, Brian, additional, Bosenberg, Adrian T., additional, Brandom, Barbara W., additional, Brett, Claire M., additional, Cain, James G., additional, Chalifoux, Thomas M., additional, Cladis, Franklyn P., additional, Cohen, David E., additional, Cohen, Ira T., additional, Cravero, Joseph P., additional, Dalesio, Nicholas M., additional, Davidson, Andrew, additional, Davis, Jessica, additional, Davis, Peter J., additional, de Souza, Duncan G., additional, Deutsch, Nina, additional, Diaz, Laura K., additional, DiNardo, James A., additional, Ehrlich, Peter F., additional, Ellis, Demetrius, additional, Fehr, James J., additional, Feldman, Jeffrey M., additional, Felmet, Kathryn, additional, Finder, Jonathan D., additional, Flack, Sean, additional, Flick, Randall P., additional, Fortier, Michelle A., additional, Frawley, Geoff, additional, Gadepalli, Samir K., additional, Galinkin, Jeffrey L., additional, Glass, Nancy, additional, Goodwin, Salvatore R., additional, Gregory, George A., additional, Grunwaldt, Lorelei, additional, Gulur, Padma, additional, Guzzetta, Nina A., additional, Haile, Dawit T., additional, Hall-Burton, Denise M., additional, Hammer, Gregory B., additional, Hamrick, Jennifer L., additional, Hamrick, Justin T., additional, Hayward, Daniel M., additional, Heitmiller, Eugenie S., additional, Herlich, Andrew, additional, Holzman, Robert S., additional, Hsieh, Vincent C., additional, Hunt, Elizabeth A., additional, Ibinson, James W., additional, Justice, Lori T., additional, Kain, Zeev N., additional, Kharasch, Evan, additional, Koka, Rahul, additional, Kost-Byerly, Sabine, additional, Krane, Elliot J., additional, Kussman, Barry D., additional, Lang, Robert Scott, additional, Lauro, Helen Victoria, additional, Lee, Jennifer K., additional, Losee, Joseph, additional, Luginbuehl, Igor, additional, Mahmoud, Mohamed, additional, Martin, Brian, additional, Mason, Keira P., additional, Mauermann, William J., additional, Maxwell, Lynne G., additional, McGowan, Francis X., additional, Miller, Bruce E., additional, Monitto, Constance L., additional, Morgan, Philip G., additional, Moritz, Michael L., additional, Motoyama, Etsuro K., additional, Nemergut, Michael E., additional, Niezgoda, Julie, additional, Ohliger, Shelley, additional, Pian, Phillip M.T., additional, Polaner, David M., additional, Politis, George D., additional, Powell, Andrew J., additional, Reynolds, Paul, additional, Ricketts, Karene, additional, Ro, Richard S., additional, Rockoff, Mark A., additional, Romanelli, Thomas, additional, Samol, Nancy Bard, additional, Samuels, Paul J., additional, Scattoloni, Joseph A., additional, McElrath Schwartz, Jamie, additional, Schwengel, Deborah A., additional, Scott, Victor L., additional, Shaffner, Donald H., additional, Shukla, Avinash C., additional, Simpao, Allan F., additional, Sivak, Erica L., additional, Sjoblom, Matthew D., additional, Soltys, Kyle, additional, Soriano, Sulpicio G., additional, Stickles, Eric T., additional, Thomas, Jennifer M., additional, Tofovic, Stevan P., additional, Tran, Kha M., additional, Trivedi, Premal M., additional, Valley, Robert D., additional, Vavilala, Monica S., additional, Vecchione, Lisa, additional, Vogt, Keith M., additional, Wahl, Jeffrey R., additional, Wahl, Kerri M., additional, Weintraub, Ari Y., additional, Welch, Timothy P., additional, Williams, Robert K., additional, Wittkugel, Eric P., additional, Woelfel, Susan, additional, Yaster, Myron, additional, Yuki, Koichi, additional, Zgleszewski, Steven, additional, Zitelli, Basil J., additional, and Zuckerberg, Aaron L., additional

Published
2017
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18. Bioelectronic Screening Platform for Real-Time Monitoring of Tumour-derived Extracellular Vesicle-Induced Epithelial-to-Mesenchymal Transition

Author

Traberg, Walther, primary, Uribe, Johana, additional, Druet, Victor, additional, Hama, Adel, additional, Moysidou, Chrysanthi-Maria, additional, Huerta, Miriam, additional, McCoy, Reece, additional, Hayward, Daniel, additional, Savva, Achilleas, additional, Genovese, Amaury, additional, Pavagada, Suraj, additional, Lu, Zixuan, additional, Koklu, Anil, additional, Pappa, Anna-Maria, additional, Fitzgerald, Rebecca, additional, Inal, Sahika, additional, Daniel, Susan, additional, and Owens, Roisin, additional

Published
2022
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23. Widespread non‐coding polymorphism in HLA class II genes of International HLA and Immunogenetics Workshop cell lines

Author

Turner, Thomas R., primary, Hayward, Daniel R., additional, Gymer, Arthur W., additional, Barker, Dominic J., additional, Leen, Gayle, additional, Cambridge, Charlotte A., additional, Macpherson, Hannah L., additional, Georgiou, Xenia, additional, Cooper, Michael A., additional, Lucas, Jonathan A. M., additional, Nadeem, Daud, additional, Robinson, James, additional, Mayor, Neema P., additional, and Marsh, Steven G. E., additional

Published
2022
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33. No Ruling, No Problem? How practitioners and fiduciaries should handle topics on which the IRS won't issue PLRs.

Author

Bosik, Joseph and Hayward, Daniel R.

Abstract

The article shares techniques on how tax planning practitioners and fiduciaries should handle potential uncertainty when the U.S. Internal Revenue Service (IRS) would not issue private letter rulings (PLR). Topics discussed are association of the no-ruling to the use of incomplete gift, non-grantor (ING) trusts and trust decanting pursuant to state law, and requirements that must be satisfied to avoid decanting resulting in the loss of goods and services tax (GST) tax-exempt status.

Published
2022

37. The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment.

Author

Legal, Thibault, Hayward, Daniel, Gluszek-Kustusz, Agata, Blackburn, Elizabeth A., Spanos, Christos, Rappsilber, Juri, Gruneberg, Ulrike, and Welburn, Julie P. I.

Subjects
*CHROMOSOMES, *CELL division, *PALMITOYLATION, *MITOSIS
Abstract

During cell division, misaligned chromosomes are captured and aligned by motors before their segregation. The CENP-E motor is recruited to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but the extent to which BubR1 contributes to CENP-E localization at kinetochores has remained controversial. Here we define the molecular determinants that specify the interaction between BubR1 and CENP-E. The basic C-terminal helix of BubR1 is necessary but not sufficient for CENP-E interaction, and a minimal key acidic patch on the kinetochore-targeting domain of CENP-E is also essential. We then demonstrate that BubR1 is required for the recruitment of CENPE to kinetochores to facilitate chromosome alignment. This BubR1– CENP-E axis is critical for alignment of chromosomes that have failed to congress through other pathways and recapitulates the major known function of CENP-E. Overall, our studies define the molecular basis and the function for CENP-E recruitment to BubR1 at kinetochores during mammalian mitosis. [ABSTRACT FROM AUTHOR]

Published
2020
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40. Mom Liked Me Better, and She Will Prove It Pre-Mortem Validation-Permanent Solution or Fad.

Author

Sneeringe, Michael, Arentz, Elizabeth A., Hayward, Daniel F., Ounanian, Melineh S., and Alaimo, Marve Ann M.

Subjects
PETITIONS
Abstract

Jane's estate plan in Ohio comprises a pour-over will and revocable trust that benefits her descendants, with the Ohio revocable trust having terms similar to the irrevocable trusts described above. For Jane, one area of caution concerns the treatment of the Delaware trusts in conjunction with the Ohio estate plan due to Ohio Rev. Code Ann. Query how an Ohio court would treat the Delaware irrevocable trusts if such process began in Delaware? Ohio would benefit from a system like Delaware's in that the approach of Delaware may curtail court involvement. [Extracted from the article]

Published
2021

41. Risk of Chondral Fracture During Implantation of Distal Femur Osteochondral Autograft Plugs: A Human Cadaveric Comparison of Four Different Donor Regions

Author

Massey, Patrick A., Hayward, Daniel, Bonner, Christian, Scalisi, Wayne, Taylor, Ryan, Vincent, Elise, W. Rutz, Robert, and F. Solitro, Giovanni

Abstract

Introduction Distal femoral cartilage lesions can be treated using osteochondral autograft transfer (OAT). When impacting plugs into a recipient site, the cartilage may fracture. This study aimed to analyze OAT donor regions and impaction energies to identify characteristics that lead to fracture.Methods Fifteen cadaver femurs were used with OAT plugs harvested from the following regions: lateral and medial trochlea (LT and MT), lateral and medial intercondylar notch (LIN and MIN). Plugs were impacted into a bone surrogate block using a custom anvil-type system with pre-determined impact heights; 30, 50, 70, and 90 mm. Each plug’s cartilage was examined and determined to be intact or fractured. Chi-square was used to compare the rate of chondral fracture for each region.Results In all, 221 plugs were included. The overall rate of chondral fracture was 45.7%. There was a significant difference in the rate of fracture between regions, with LIN, MIN, LT, and MT, having a fracture rate of 46.6%, 62.7%, 25.0%, and 51.9%, respectively (P= 0.001). An impact height of 30 mm resulted in a fracture rate of 17.7%. Increasing the impact height from 30 to 50 mm resulted in significantly increased chondral fracture risk (P= 0.001).Conclusion Different donor regions have varying rates of chondral fracture during OAT plug impaction, with the lateral and medial trochlea being the most resistant to chondral fracture at lower forces. Increased impact energy increases risk of chondral fracture. Surgeons should maintain caution and utilize lower impact energy when inserting OAT plugs.

Published
2024
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42. Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint

Author

Zhang, Gang, Lischetti, Tiziana, Hayward, Daniel G, Nilsson, Jakob, Zhang, Gang, Lischetti, Tiziana, Hayward, Daniel G, and Nilsson, Jakob

Abstract

The spindle assembly checkpoint (SAC) ensures proper chromosome segregation by delaying anaphase onset in response to unattached kinetochores. Checkpoint signalling requires the kinetochore localization of the Mad1-Mad2 complex that in more complex eukaryotes depends on the Rod-Zwilch-ZW10 (RZZ) complex. The kinetochore protein Zwint has been proposed to be the kinetochore receptor for RZZ, but here we show that Bub1 and not Zwint is required for RZZ recruitment. We find that the middle region of Bub1 encompassing a domain essential for SAC signalling contributes to RZZ localization. In addition, we show that a distinct region in Bub1 mediates kinetochore localization of BubR1 through direct binding, but surprisingly removal of this region increases checkpoint strength. Our work thus uncovers how Bub1 coordinates checkpoint signalling by distinct domains for RZZ and BubR1 recruitment and suggests that Bub1 localizes antagonistic checkpoint activities.

Published
2015

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