1. Synthesis and application of a highly selective copper ions fluorescent probe based on the coumarin group.
- Author
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He, Guangjie, Liu, Xiangli, Xu, Jinhe, Ji, Liguo, Yang, Linlin, Fan, Aiying, Wang, Songjun, and Wang, Qingzhi
- Subjects
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COUMARINS , *SCHIFF bases , *COPPER ions , *CELL imaging , *FLUORESCENT probes - Abstract
A highly selective copper ions fluorescent probe based on the coumarin-type Schiff base derivative 1 (probe) was produced by condensation reaction between coumarin carbohydrazide and 1H-indazole-3-carbaldehyde. The UV–vis spectroscopy showed that the maximum absorption peak of compound 1 appeared at 439 nm. In the presence of Cu 2 + ions, the maximum peak decreased remarkably compared with other physiological important metal ions and a new absorption peak at 500 nm appeared. The job's plot experiments showed that complexes of 1:2 binding mode were formed in CH 3 CN:HEPES (3:2, v/v) solution. Compound 1 exhibited a strong blue fluorescence. Upon addition of copper ions, the fluorescence gradually decreased and reached a plateau with the fluorescence quenching rate up to 98.73%. The detection limit for Cu 2 + ions was estimated to 0.384 ppm. Fluorescent microscopy experiments demonstrated that probe 1 had potential to be used to investigate biological processes involving Cu 2 + ions within living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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