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Objective To observe the regulatory effect of exosomes from adipose-derived stem cells （ADSC-Exos） on the biological activity of scar fibroblasts. Methods Scar fibroblast were prepared and divided into the control and ADSC-Exos groups. The proliferation of scar fibroblasts between two groups was observed by CCK-8 assay. The expression levels of fibrosis-related indexes were detected by Western blot. The expression levels of fibrosis-related gene mRNA were determined by fluorescent quantitative real-time （RT） -PCR. Results Compared with the control group, the proliferation capability of scar fibroblasts was significantly weakened, and the fibrosis-related indexes were significantly decreased in ADSC-Exos group （both P < 0.05）. Meanwhile, the expression levels of collagen I, III and α-smooth muscle actin mRNA of scar fibroblasts were significantly down-regulated in the ADSC-Exos group（all P < 0.05）. Conclusion ADSC-Exos can inhibit the biological activity of scar fibroblasts.

This research was set up to determine the efficacy of a synthetic biomimetic skin substitute of poly-L-lactide (PLLA)/gelatin nanofiber membrane in facilitating chronic cutaneous wound healing. The material physical properties and biological characteristics were evaluated. The safety and effectiveness were further confirmed by clinical applications. Results indicated that the average diameter of the nano pores in the biomimetic substitute was 0.65 μm. The mean contact angle was 80.39°, and the average tensile strength was 4.42 ± 0.43 MPa. The proliferation of fibroblasts and monocytes was significantly improved at day 7 cultured on the substitute. Moreover, the bacterial adhesion on the substitute was decreased. In the clinical case study, the percentage of complete wound healing was 93.3%, and the rate of reoperation was 6.7%. Thus, this technology presents an intriguing possible therapeutic modality that provides pain relief and diminishes the burden of reoperation in the setting of chronic wound management. [ABSTRACT FROM AUTHOR]

Fibrous encapsulation that prevents the direct contact between an implant and the bone can cause implant failure. However, prevention of fibrous encapsulation is difficult because of the lack of effective strategies which can selectively control the growth of fibroblasts and osteoblasts. Because curcumin, an extract from Curcuma longa, was recently found to reduce the formation of fibrous tissue, it is hypothesized that loading curcumin on implant surfaces would be efficacious in inhibiting fibrous encapsulation without adversely affecting the osteoblast functions. To prove this hypothesis, curcumin was loaded on to a titanium surface using poly(dopamine) as an anchor, and the behaviors of fibroblasts and osteoblasts on these curcumin-modified surfaces were investigated. Curcumin was successfully loaded on to titanium and showed a low release after incubation in phosphate-buffered saline for seven days. On the curcumin-modified surfaces, fibroblast proliferation was suppressed, and fibrous marker expressions as well as collagen synthesis were significantly reduced. These reductions were possibly because of the enhancement of fibroblast apoptosis induced by the surface curcumin. In contrast, no significant reduction in osteoblast functions was observed on the curcumin-modified substrates. These findings may provide a promising solution to reduce fibrous encapsulation, and thus may be highly beneficial for orthopaedic applications. [ABSTRACT FROM AUTHOR]