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116 results on '"He, Rongquan"'

16. Genetic ablation of Pim1 or pharmacologic inhibition with TP-3654 ameliorates myelofibrosis in murine models

Author

Dutta, Avik, primary, Nath, Dipmoy, additional, Yang, Yue, additional, Le, Bao T., additional, Rahman, Mohammad Ferdous-Ur, additional, Faughnan, Patrick, additional, Wang, Zhenjia, additional, Stuver, Matthew, additional, He, Rongquan, additional, Tan, Wuwei, additional, Hutchison, Robert E., additional, Foulks, Jason M., additional, Warner, Steven L., additional, Zang, Chongzhi, additional, and Mohi, Golam, additional

Published
2021
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21. Supplemental Material, sj-docx-2-tct-10.1177_15330338211024551 - Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma

Author

Li, Ruolin, Chen, Gang, Dang, Yiwu, He, Rongquan, Liu, Angui, Ma, Jie, and Ling, Zhian

Subjects
110320 Radiology and Organ Imaging, FOS: Clinical medicine, Biochemistry, 111299 Oncology and Carcinogenesis not elsewhere classified
Abstract

Supplemental Material, sj-docx-2-tct-10.1177_15330338211024551 for Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma by Ruolin Li, Gang Chen, Yiwu Dang, Rongquan He, Angui Liu, Jie Ma and Zhian Ling in Technology in Cancer Research & Treatment

Published
2021
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22. Supplemental Material, sj-docx-1-tct-10.1177_15330338211024551 - Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma

Author

Li, Ruolin, Chen, Gang, Dang, Yiwu, He, Rongquan, Liu, Angui, Ma, Jie, and Ling, Zhian

Subjects
110320 Radiology and Organ Imaging, FOS: Clinical medicine, Biochemistry, 111299 Oncology and Carcinogenesis not elsewhere classified
Abstract

Supplemental Material, sj-docx-1-tct-10.1177_15330338211024551 for Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma by Ruolin Li, Gang Chen, Yiwu Dang, Rongquan He, Angui Liu, Jie Ma and Zhian Ling in Technology in Cancer Research & Treatment

Published
2021
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24. MiR-30a-5p suppresses cell growth and enhances apoptosis of hepatocellular carcinoma cells via targeting AEG-1

Author

He, Rongquan, Yang, Lihua, Lin, Xiaomiao, Chen, Xin, Lin, Xinggu, Wei, Fanglin, Liang, Xiaona, Luo, Yihuan, Wu, Yuzhuang, Gan, Tingqing, Dang, Yiwu, and Chen, Gang

Subjects
Caspase 7, Binding Sites, Carcinoma, Hepatocellular, Time Factors, Caspase 3, Cell Survival, Liver Neoplasms, Membrane Proteins, RNA-Binding Proteins, Apoptosis, Hep G2 Cells, Transfection, Gene Expression Regulation, Neoplastic, MicroRNAs, Genes, Reporter, Humans, Original Article, RNA Interference, Erratum, 3' Untranslated Regions, Cell Adhesion Molecules, Cell Proliferation, Signal Transduction
Abstract

Background: MiR-30a-5p has been reported to play vital roles in the carcinogenesis and progression of various malignancies via different molecular mechanisms. However, the role and target genes of miR-30a-5p in hepatocellular carcinoma (HCC) remain still unclear. In silico analysis finds that there are complementary sequences between the 3’-untrasnlated region of astrocyte elevated gene 1 (AEG-1) and miR-30a-5p. Herein, we investigated the biological function of miR-30a-5p, as well as the potential molecular mechanism via targeting AEG-1 in HCC cells. Materials and methods: MiR-30a-5p inhibitor, miR-30a-5p mimic, AEG-1 siRNAs, as well as their negative controls were transfected into HCC cell lines HepG2, SMMC-7221, HepB3 and SNU449. Then, the in vitro influence and mechanism of miR-30a-5p on cell viability, proliferation, caspase-3/7 activity and apoptosis were studied, as assessed by different methods, including spectrophotometry, fluorimetry, fluorescence microscopy of Hoechst 33342/propidium iodide double chromatin staining, western blot and dual luciferase reporter assay, respectively. Results: MiR-30a-5p mimic markedly inhibited cell growth, also induced caspase-3/7 activity and apoptosis in all four HCC cell lines tested. The strongest effect was observed in HepG2 and SMMC-7721 cells. However, this effect was slightly weaker than that of AEG-1 siRNAs. Transfection of miR-30a-5p mimic led to a markedly reduced AEG-1 protein level and further dual luciferase reporter assay confirmed that AEG-1 was one of the target genes of miR-30a-5p in HCC cells. Conclusions: MiR-30a-5p may play an essential role in the cell growth and apoptosis of HCC cells, partially via targeting AEG-1.

Published
2020

29. Upregulation of ATIC in multiple myeloma tissues based on tissue microarray and gene microarrays.

Author

Li, Ruolin, Chen, Gang, Dang, Yiwu, He, Rongquan, Liu, Angui, Ma, Jie, and Wang, Chengbin

Subjects
TISSUE arrays, PROTEINS, SEQUENCE analysis, IMMUNOHISTOCHEMISTRY, MICROARRAY technology, REGRESSION analysis, GENE expression, IMIDAZOLES, CELLULAR signal transduction, T-test (Statistics), TRANSFERASES, MESSENGER RNA, DESCRIPTIVE statistics, SURVIVAL analysis (Biometry), MULTIPLE myeloma, ODDS ratio, DATA analysis software, RECEIVER operating characteristic curves, PROPORTIONAL hazards models
Abstract

Purpose: Multiple myeloma (MM) is characterized by the malignant proliferation of plasma cells, which produce a monoclonal immunoglobulin protein. The role of 5‐aminoimidazole‐4‐carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) has not yet been well studied in the area of MM. Thus, in the current study, we sought to examine the expression levels, including mRNA and protein levels of ATIC in MM. Methods: Multiple myeloma microarray and RNA‐seq data were screened from the SRA, GEO, ArrayExpress, and Oncomine databases. The mRNA level of ATIC was extracted from the high throughput data, and the prognostic value was studied. The protein level of ATIC was also detected by in‐house immunohistochemistry on a tissue microarray. Potential signaling pathways were enriched with ATIC‐related genes in MM. Results: Both the mRNA and protein levels of ATIC were significantly upregulated in MM samples as compared to normal samples. Furthermore, the summarized Standardized Mean Difference was 1.66 with 674 cases of MM based on 10 independent studies including the in‐house tissue microarray. The overall hazard ratio of ATIC in MM was 1.7 with 1631 cases of MM based on five microarrays. In the KEGG pathway analysis, the ATIC‐related genes were mainly enriched in the pathway of complement and coagulation cascades. Conclusion: We provided the first evidence supporting the upregulation of ATIC may play an essential part in the tumorigenesis and development of MM. The promoting cancer capacity may be related to the pathway of complement and coagulation cascades. [ABSTRACT FROM AUTHOR]

Published
2021
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30. Polyadenylation of Histone H3.1 mRNA Promotes Cell Transformation by Displacing H3.3 from Gene Regulatory Elements

Author

Chen, Danqi, primary, Chen, Qiao Yi, additional, Wang, Zhenjia, additional, Zhu, Yusha, additional, Kluz, Thomas, additional, Tan, Wuwei, additional, Li, Jinquan, additional, Wu, Feng, additional, Fang, Lei, additional, Zhang, Xiaoru, additional, He, Rongquan, additional, Shen, Steven, additional, Sun, Hong, additional, Zang, Chongzhi, additional, Jin, Chunyuan, additional, and Costa, Max, additional

Published
2019
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31. The essential role of MTDH in the progression of HCC: a study with immunohistochemistry, TCGA, meta-analysis and in vitro investigation

Author

He, Rongquan, Gao, Li, Ma, Jie, Peng, Zhigang, Zhou, Shengsheng, Yang, Lihua, Feng, Zhenbo, Dang, Yiwu, and Chen, Gang

Subjects
Original Article, neoplasms, digestive system diseases
Abstract

Recent studies found that metadherin (MTDH) played an essential role in hepatocellular carcinoma (HCC). Nevertheless, the exact function of MTDH in the pathogenesis of HCC was unclarified. In the present study, we aimed to investigate the clinical significance of MTDH in HCC and its effect on HCC cells. Immunohistochemistry (IHC) was performed to detect MTDH expression in HCC tissues. Data from The Cancer Genome Atlas (TCGA) and ONCOMINE was obtained to examine MTDH expression in HCC and its clinical significance. Meta-analysis was conducted to assess the correlation between MTDH expression and both the prognosis (Overall Survival (OS) or Disease-free Survival (DFS)) and clinicopathological features of HCC via STATA 12.0. In vitro experiments were performed to investigate the role of MTDH in cell growth, caspase-3/7 activity and apoptosis in HCC cells. The MTDH staining was remarkably stronger in HCC tissues than in non-cancer tissues from IHC, TCGA and ONCOMINE data. Moreover, MTDH-positive expression was significantly correlated with pathological grade, distant metastasis and hepatitis B virus (HBV) infection by IHC. For meta-analysis, MTDH expression was indicative of poor OS without heterogeneity in HCC patients. Additionally, MTDH expression was correlated with high-grade histological differentiation, non-vascular invasion and metastasis in HCC. In vitro experiments revealed that MTDH could the inhibit cell growth and activate caspase-3/7 activity and apoptosis in the four HCC cell lines. In conclusion, MTDH expression may serve as a novel targeting strategy for HCC due to its clinical significance and oncogenic function in HCC cells.

Published
2017

33. Prognostic and Clinicopathological Significance of Downregulated p16 Expression in Patients with Bladder Cancer: A Systematic Review and Meta-Analysis

Author

Gan, Xiaoning, Lin, Xiaomiao, He, Rongquan, Lin, Xinggu, Wang, Hanlin, Yan, Liyan, Zhou, Hong, Qin, Hui, and Chen, Gang

Subjects
Article Subject
Abstract

p16, encoded by the CDKN2A gene, is a tumor suppressor that has been widely studied in cancer research. However, the relationship of p16 with prognostic and clinicopathological parameters in patients with bladder cancer remains unclear. Data inclusion criteria were articles reporting on the relationship between p16 expression and the prognosis or clinicopathology in patients with bladder cancer. Meta-analyses were performed with Stata software. Hazard ratios (HRs) or odds ratios (ORs) and 95% confidence intervals (95% CI) were calculated to evaluate the relative risks. The source of heterogeneity was analyzed by subgroup analysis. A total of 37 studies with 2246 cases were included and analyzed. The results identified an important link between downregulated p16 expression and poor prognosis in patients with bladder cancer in terms of recurrence-free survival (RFS), overall survival (OS), progression-free survival (PFS), and some clinicopathological parameters including clinical staging, pathological degree, and lymph node metastasis. Subgroup analysis also showed that low p16 expression could function as a warning sign for RFS and PFS in patients with early-stage (Ta–T1) bladder cancer. In conclusion, p16 might play an essential role in the deterioration of bladder cancer and could serve as a biomarker for the prediction for patients’ progression and prognosis.

Published
2016
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34. Low Serum Sex Hormone-Binding Globulin Associated with Insulin Resistance in Men with Nonalcoholic Fatty Liver Disease

Author

Ye, Juan, additional, Yao, Ziting, additional, Tan, Aihua, additional, Gao, Yong, additional, Chen, Yingchun, additional, Lin, Xinggu, additional, He, Rongquan, additional, Tang, Ruiqiang, additional, Hu, Yanling, additional, Zhang, Haiying, additional, Yang, Xiaobo, additional, Wang, Qiuyan, additional, Jiang, Yonghua, additional, and Mo, Zengnan, additional

Published
2017
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35. Clinicopathological role of miR-30a-5p in hepatocellular carcinoma tissues and prediction of its function with bioinformatics analysis

Author

Huang,Wenting, Chen,Zu-xuan, He,Rongquan, Wu,Yuzhuang, Yin,Shuya, Liang,Xiao-na, Chen,Gang, Yang,Hong, Peng,Zhi-gang, Yang,Lihua, Huang,Wenting, Chen,Zu-xuan, He,Rongquan, Wu,Yuzhuang, Yin,Shuya, Liang,Xiao-na, Chen,Gang, Yang,Hong, Peng,Zhi-gang, and Yang,Lihua

Abstract

Wen-Ting Huang,1,* Zu-xuan Chen,2,* Rong-quan He,2 Yu-zhuang Wu,1 Shu-ya Yin,1 Xiao-na Liang,1 Gang Chen,1 Hong Yang,3 Zhi-gang Peng,2,* Li-hua Yang2,* 1Department of Pathology, 2Department of Medical Oncology, 3Department of Ultrasonography, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People’s Republic of China *These authors contributed equally to this work Background: It has been reported that deregulation or dysfunction of microRNAs (miRNAs) plays an essential part in the hepatocarcinogenesis. However, the contribution and mechanism of microRNA-30a-5p (miR-30a-5p) in hepatocellular carcinoma (HCC) remains largely unknown. Therefore, our aim was to investigate the clinicopathological role of miR-30a-5p in HCC tissues and explore its potential pathways in this study.Methods: The expression of miR-30a-5p was measured in 95 HCC and adjacent noncancer tissues by real-time reverse transcription quantitative polymerase chain reaction. The relationship between miR-30a-5p expression levels and clinicopathological parameters was also analyzed. Furthermore, the potential target genes of miR-30a-5p were collected via online prediction and literature searching. Gene ontology and pathway enrichment analyses were used to identify the possible function of miR-30a-5p in HCC.Results: Compared with adjacent noncancer tissues (2.23±0.77), expression level of miR-30a-5p was significantly lower in HCC tissues (1.26±0.66, P<0.001). MiR-30a-5p expression was evidently correlated with tumor nodes, metastasis, tumor–node–metastasis stage, portal vein tumor embolus, vascular invasion, and status of tumor capsule (all P<0.05). A total of 878 genes were finally used for the biological informatics analyses. These prospective target genes were highly enriched in various key pathways, for instance, Ubiquitin-mediated proteolysis, Axon guidance, Ne

Published
2016

48. Expression and clinicopathological significance of miR-146a in hepatocellular carcinoma tissues.

Author

Rong, Minhua, He, Rongquan, Dang, Yiwu, and Chen, Gang

Subjects
*MICRORNA, *GENE expression, *LIVER cancer, *ALKANES, *FORMALDEHYDE
Abstract

Background. Aberrant expression of microRNA-146a (miR-146a) has been found in several classes of cancers. However, its expression and clinicopathological contribution in hepatocellular carcinoma (HCC) has not been fully elucidated. Objective. To explore the clinicopathological significance of the miR-146a level in HCC formalin-fixed paraffin-embedded (FFPE) tissue. Methods. Eighty-five HCC samples and their para-cancerous normal liver tissues were collected. Total mRNA including miRNA was extracted, and miR-146a expression was determined using real-time RT-PCR. Furthermore, the correlation between the miR-146a expression and clinicopathological parameters was investigated. Results. MicroRNA-146a expression in HCC tissues was lower compared with that in adjacent non-cancerous hepatic tissues. MicroRNA-146a expression was also related to clinical TNM stage, metastasis, portal vein tumor embolus, and number of tumor nodes. Conclusions. Down-regulation of miR-146a is related to HCC carcinogenesis and deterioration of HCC. MicroRNA-146a may act as a suppressor miRNA of HCC, and it is therefore a potential prognostic biomarker for HCC patients. [ABSTRACT FROM AUTHOR]

Published
2014
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49. Neutrophil Extracellular Traps: Potential Thrombotic Markers and Therapeutic Targets in Colorectal Cancer.

Author

Huang X, He R, Jiang Y, Tang J, Xu X, Laoguo S, Chen G, and Ma J

Abstract

Neutrophil extracellular traps (NETs) are promising promoters in venous thromboembolism (VTE). In the present study, we have investigated the potential thrombogenic role of NETs in colorectal cancer (CRC). A total of 583 patients with gastrointestinal malignancies who were diagnosed with or without VTE by extremities arteriovenous ultrasound and computed tomography were enrolled. The incidence of VTE in CRC was as high as 17.53%. In serological ELISA experiments, Cit-H3, MPO and cfDNA were significantly overexpressed in CRC patients with VTE compared to CRC patients without VTE and healthy individuals. Neutrophils from CRC patients with VTE produced appreciable amounts of NETs after stimulation with phorbol-12-myristate-13-acetate, which were lacking in CRC patients without VTE and healthy individuals. CfDNA was positively correlated with plasmin-α2-antiplasmin complex and tissue plasmin activator inhibitor-1 complex, and Cit-H3 was positively correlated with plasmin-α2-antiplasmin complex, suggesting that NETs are associated with increased fibrinolytic activity. We screened some NETs-related genes by analysing several high-throughput sequencing datasets of VTE and NETs. FCGR1A was identified as the optimal target gene by pan-cancer expression analysis and survival analysis. FCGR1A was significantly overexpressed in the peripheral blood of CRC patients without VTE compared to healthy individuals and showed a positive correlation with cfDNA. Neutrophil-derived NETs were significantly reduced by FCGR1A inhibitor exposure. These findings indicate that NETs are actively involved in VTE in CRC. NETs are promising thrombotic marker and therapeutic target in CRC to prevent the thrombotic consequences of cancer., (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for Leukocyte Biology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Published
2024
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