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3. Pyridinium-conjugated polynorbornenes for nanomolar ATP sensing using an indicator displacement assay and a PET strategy.

Author

Huang, Ling-Xi, He, Yue-Bo, Kim, Jaewon, Sharma, Amit, Cao, Qian-Yong, and Kim, Jong Seung

Subjects
*FUNCTIONAL groups, *FLUORESCENCE
Abstract

An indicator displacement assay, namely polymeric PNPY-n/UD consisting of a cationic polynorbornene backbone with pyridinium functional groups (PNPY-1,2,3) and an anionic uranine dye (UD) as an indicator, has been developed for highly sensitive "turn-on" fluorescence sensing of ATP. While PNPY-1/UD itself is non-emissive, a bright green fluorescence signal was observed in the presence of ATP [Ka = 2.17 × 105 M−1, LOD = 5.7 nM]. The potential of a highly photostable system PNPY-1/UD was also validated in detecting ATP levels in live-cell imaging applications. [ABSTRACT FROM AUTHOR]

Published
2021
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