Search

Reports on the regulation region of larval serum protein gene, Bombyx mori, (BmLSP). Indication that the first intron, the first exon, the central promoter region and 5'-upstream region, is cloned from genomic DNA of the silkworm, SujuxMinghu; Use of PCR and restriction endonuclease methods; Construction of a series of luciferase reporter plasmids, driven by different length of BmLSP promoters; Investigation into the regulation elements and foreign insect hormones' effect on the BmLSP promoter activity, via the transient expression system in BmN cells; Finding that the BmLSP promoter activity is higher; Result when the first intron or the element in 5'-upstream region harboring the homologous sequence with the first intron of light-chain fibroin gene (EHIF) is deleted; Suggestion that the first intron and EHIF contain the main positive cis-acting elements; The negative effect of the inactive mariner transposable element (MTE); Effects of juvenile hormone analogue (JHA) on the BmLSP promoter activity demonstrating a dose-dependent manner; Idea that low concentration treatments increase the BmLSP promoter activity, while high concentration treatments decrease it; Assertion that insect ecdysone (MH) treatments present no significant effect.