1. Molecular characterization and glomalin production of arbuscular mycorrhizal fungi colonizing a heavy metal polluted ash disposal island, downtown Venice
- Author
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Chiarafrancesca Rigo, Stefano Bedini, Alessandra Turrini, Emanuele Argese, and Manuela Giovannetti
- Subjects
Rhizosphere ,Glomalin ,GRSP ,biology ,fungi ,Soil Science ,Ribosomal RNA ,biology.organism_classification ,Microbiology ,Colonisation ,Glomeromycota ,Heavy metals ,Ash dump ,MSW bottom ashes ,Arbuscular mycorrhizas ,AMF diversity ,Soil water ,Botany ,biology.protein ,Colonization ,Mycorrhiza ,Heavy metals, Ash dump, MSW bottom ashes, Arbuscular mycorrhizas, AMF diversity, GRSP, Glomalin - Abstract
In this work the arbuscular mycorrhizal (AM) fungal communities colonizing a polluted ash dump island, downtown Venice, were studied by using a multimodal approach. The island, Sacca San Biagio, was covered with a thick layer of municipal solid waste residues produced by an incinerator operating from 1973, to 1984. Such residues contained high levels of heavy metals (Cu, Pb and Zn). We characterized the AMF communities present in soils on Sacca San Biagio island by using molecular methods. Nine AM fungal sequence types were detected in the roots of three plant species, representative of the dominant flora, by using partial SSU ribosomal RNA genes. The most abundant sequence types corresponded to Glomus intraradices / Glomus fasciculatum , and to Glo18, a sequence detected so far only in planta . Two sequences were new to science. Glomalin-related soil protein (GRSP), extracted from rhizosphere soil of dominant plant species, ranged from 1.6 to 2.3 mg g −1 . The occurrence of an active AM fungal community able to live in such harsh environment was evinced by the correlation between mycorrhizal colonization and GRSP content.
- Published
- 2010