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151 results on '"Heddleston, John M."'

1. Actin cables and comet tails organize mitochondrial networks in mitosis

Author

Moore, Andrew S., Coscia, Stephen M., Simpson, Cory L., Ortega, Fabian E., Wait, Eric C., Heddleston, John M., and Nirschl, Jeffrey J.

Subjects
Mitosis -- Physiological aspects, Actin -- Structure, Mitochondria -- Distribution, Company distribution practices, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Symmetric cell division requires the even partitioning of genetic information and cytoplasmic contents between daughter cells. Whereas the mechanisms coordinating the segregation of the genome are well known, the processes that ensure organelle segregation between daughter cells remain less well understood.sup.1. Here we identify multiple actin assemblies with distinct but complementary roles in mitochondrial organization and inheritance in mitosis. First, we find a dense meshwork of subcortical actin cables assembled throughout the mitotic cytoplasm. This network scaffolds the endoplasmic reticulum and organizes three-dimensional mitochondrial positioning to ensure the equal segregation of mitochondrial mass at cytokinesis. Second, we identify a dynamic wave of actin filaments reversibly assembling on the surface of mitochondria during mitosis. Mitochondria sampled by this wave are enveloped within actin clouds that can spontaneously break symmetry to form elongated comet tails. Mitochondrial comet tails promote randomly directed bursts of movement that shuffle mitochondrial position within the mother cell to randomize inheritance of healthy and damaged mitochondria between daughter cells. Thus, parallel mechanisms mediated by the actin cytoskeleton ensure both equal and random inheritance of mitochondria in symmetrically dividing cells. During mitosis, complementary actin-based mechanisms ensure equal and random distributions of mitochondria among daughter cells following symmetrical cell division., Author(s): Andrew S. Moore [sup.1] [sup.2] [sup.3] , Stephen M. Coscia [sup.1] [sup.2] [sup.4] , Cory L. Simpson [sup.1] [sup.2] [sup.5] , Fabian E. Ortega [sup.6] , Eric C. Wait [...]

Published
2021
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3. High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

Author

Camp Jr., Charles H., Lee, Young Jong, Heddleston, John M., Hartshorn, Christopher M., Walker, Angela R. Hight, Rich, Jeremy N., Lathia, Justin D., and Cicerone, Marcus T.

Subjects
Physics - Optics, Physics - Instrumentation and Detectors
Abstract

We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser sources that probes the Raman spectrum over 3,000 cm$^{-1}$ and generates an especially strong response in the typically weak Raman "fingerprint" region through heterodyne amplification of the anti-Stokes photons with a large nonresonant background (NRB) while maintaining high spectral resolution of $<$ 13 cm$^{-1}$. For histology and pathology, this system shows promise in highlighting major tissue components in a non-destructive, label-free manner. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumors and the surrounding healthy brain matter., Comment: 22 pages (11 main, 11 supplemental), 9 figures (4 main, 5 supplemental; N.B.: reduced resolution for smaller file size-- contact author for full resolution images). This paper was submitted to Nature Photonics on October 2, 2013 (minor corrections applied). This version concatenates the supplementary section to the end of the main text-- rather than a separate file-- No document changes otherwise

Published
2014
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4. BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis

Author

McArthur, Kate, Whitehead, Lachlan W., Heddleston, John M., Li, Lucy, Padman, Benjamin S., Oorschot, Viola, Geoghegan, Niall D., Chappaz, Stephane, Davidson, Sophia, San Chin, Hui, Lane, Rachael M., Dramicanin, Marija, Saunders, Tahnee L., Sugiana, Canny, Lessene, Romina, Osellame, Laura D., Chew, Teng-Leong, Dewson, Grant, Lazarou, Michael, Ramm, Georg, Lessene, Guillaume, Ryan, Michael T., Rogers, Kelly L., van Delft, Mark F., and Kile, Benjamin T.

Published
2018

5. Supplementary Table 6 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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6. Supplementary Table 3 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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7. Supplementary Table 1 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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8. Supplementary Table 5 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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9. Supplementary Table 7 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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10. Supplementary Figures 1-12 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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11. Supplementary Methods, Figures 1-11, Table Legends 1-7 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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12. Data from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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13. Supplementary Table 4 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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14. Supplementary Table 2 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells

Author

Mathieu, Julie, primary, Zhang, Zhan, primary, Zhou, Wenyu, primary, Wang, Amy J., primary, Heddleston, John M., primary, Pinna, Claudia M.A., primary, Hubaud, Alexis, primary, Stadler, Bradford, primary, Choi, Michael, primary, Bar, Merav, primary, Tewari, Muneesh, primary, Liu, Alvin, primary, Vessella, Robert, primary, Rostomily, Robert, primary, Born, Donald, primary, Horwitz, Marshall, primary, Ware, Carol, primary, Blau, C. Anthony, primary, Cleary, Michele A., primary, Rich, Jeremy N., primary, and Ruohola-Baker, Hannele, primary

Published
2023
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16. The Cancer Stem Cell Paradigm

Author

Eyler, Christine E., Heddleston, John M., Hitomi, Masahiro, Guryanova, Olga A., Macswords, Jennifer M., Flavahan, William A., Hjelmeland, Anita B., Rich, Jeremy N., and Phinney, Donald G., editor

Published
2011
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17. Nonreceptor Tyrosine Kinase BMX Maintains Self-Renewal and Tumorigenic Potential of Glioblastoma Stem Cells by Activating STAT3

Author

Guryanova, Olga A., Wu, Qiulian, Cheng, Lin, Lathia, Justin D., Huang, Zhi, Yang, Jinbo, MacSwords, Jennifer, Eyler, Christine E., McLendon, Roger E., Heddleston, John M., Shou, Weinian, Hambardzumyan, Dolores, Lee, Jeongwu, Hjelmeland, Anita B., Sloan, Andrew E., Bredel, Markus, Stark, George R., Rich, Jeremy N., and Bao, Shideng

Published
2011
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19. Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration

Author

Arts, Janine JG, primary, Mahlandt, Eike K, additional, Grönloh, Max LB, additional, Schimmel, Lilian, additional, Noordstra, Ivar, additional, Gordon, Emma, additional, van Steen, Abraham CI, additional, Tol, Simon, additional, Walzog, Barbara, additional, van Rijssel, Jos, additional, Nolte, Martijn A, additional, Postma, Marten, additional, Khuon, Satya, additional, Heddleston, John M, additional, Wait, Eric, additional, Chew, Teng Leong, additional, Winter, Mark, additional, Montanez, Eloi, additional, Goedhart, Joachim, additional, and van Buul, Jaap D, additional

Published
2021
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20. Author response: Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration

Author

Arts, Janine JG, primary, Mahlandt, Eike K, additional, Grönloh, Max LB, additional, Schimmel, Lilian, additional, Noordstra, Ivar, additional, Gordon, Emma, additional, van Steen, Abraham CI, additional, Tol, Simon, additional, Walzog, Barbara, additional, van Rijssel, Jos, additional, Nolte, Martijn A, additional, Postma, Marten, additional, Khuon, Satya, additional, Heddleston, John M, additional, Wait, Eric, additional, Chew, Teng Leong, additional, Winter, Mark, additional, Montanez, Eloi, additional, Goedhart, Joachim, additional, and van Buul, Jaap D, additional

Published
2021
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25. Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration

Author

Arts, Janine J.G., primary, Mahlandt, Eike K., additional, Grönloh, Max L.B., additional, Schimmel, Lilian, additional, Noordstra, Ivar, additional, van Steen, Abraham C.I., additional, Tol, Simon, additional, van Rijssel, Jos, additional, Nolte, Martijn A., additional, Postma, Marten, additional, Khuon, Satya, additional, Heddleston, John M., additional, Wait, Eric, additional, Chew, Teng-Leong, additional, Winter, Mark, additional, Montanez, Eloi, additional, Goedhart, Joachim, additional, and van Buul, Jaap D., additional

Published
2021
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29. FMNL2 regulates dynamics of fascin in filopodia

Author

Pfisterer, Karin, primary, Levitt, James, additional, Lawson, Campbell D., additional, Marsh, Richard J., additional, Heddleston, John M., additional, Wait, Eric, additional, Ameer-Beg, Simon Morris, additional, Cox, Susan, additional, and Parsons, Maddy, additional

Published
2020
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30. Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis

Author

Sato, Yuko, primary, Hilbert, Lennart, additional, Oda, Haruka, additional, Wan, Yinan, additional, Heddleston, John M., additional, Chew, Teng-Leong, additional, Zaburdaev, Vasily, additional, Keller, Philipp, additional, Lionnet, Timothee, additional, Vastenhouw, Nadine, additional, and Kimura, Hiroshi, additional

Published
2019
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31. Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor

Author

O’Shaughnessy, Ellen C., primary, Stone, Orrin J., additional, LaFosse, Paul K., additional, Azoitei, Mihai L., additional, Tsygankov, Denis, additional, Heddleston, John M., additional, Legant, Wesley R., additional, Wittchen, Erika S., additional, Burridge, Keith, additional, Elston, Timothy C., additional, Betzig, Eric, additional, Chew, Teng-Leong, additional, Adalsteinsson, David, additional, and Hahn, Klaus M., additional

Published
2019
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37. A Moving Source of Matrix Components Is Essential for De Novo Basement Membrane Formation

Author

Matsubayashi, Yutaka, Louani, Adam, Dragu, Anca, Sanchez Sanchez, Besaiz J, Serna Morales, Eduardo, Yolland, Lawrence, György, Attila, Vizcay, Gema, Fleck, Roland A, Heddleston, John M, Chew, Teng-Leong, Siekhaus, Daria E, and Stramer, Brian M

Subjects
576 Genetics and evolution, hemocyte, cell migration, extracellular matrix, Macrophages, morphogenesis, Epithelial Cells, macrophage, basement membrane, Article, perlecan, Drosophila melanogaster, laminin, Cell Movement, collagen IV, 570 Life sciences, biology, Animals, Drosophila Proteins, Drosophila, Collagen
Abstract

Summary The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is unknown how all of these components are generated and subsequently constructed to form a fully mature BM in the living animal. Although BM formation is thought to simply involve a process of self-assembly [2], this concept suffers from a number of logistical issues when considering its construction in vivo. First, incorporation of BM components appears to be hierarchical [3, 4, 5], yet it is unclear whether their production during embryogenesis must also be regulated in a temporal fashion. Second, many BM proteins are produced not only by the cells residing on the BM but also by surrounding cell types [6, 7, 8, 9], and it is unclear how large, possibly insoluble protein complexes [10] are delivered into the matrix. Here we exploit our ability to live image and genetically dissect de novo BM formation during Drosophila development. This reveals that there is a temporal hierarchy of BM protein production that is essential for proper component incorporation. Furthermore, we show that BM components require secretion by migrating macrophages (hemocytes) during their developmental dispersal, which is critical for embryogenesis. Indeed, hemocyte migration is essential to deliver a subset of ECM components evenly throughout the embryo. This reveals that de novo BM construction requires a combination of both production and distribution logistics allowing for the timely delivery of core components., Highlights • Macrophages are major producers of basement membrane in the Drosophila embryo • Basement membrane components require hierarchical deposition during development • Macrophage migration is essential to evenly deliver a subset of matrix components • Uneven macrophage dispersal leads to uneven matrix incorporation and lethality, Drosophila macrophages undergo stereotyped developmental migrations to evenly populate the embryo. Matsubayashi et al. reveal that macrophages are the biggest producers of extracellular matrix in the embryo and that their dispersal is essential to evenly distribute a subset of matrix components during development.

Published
2017

40. Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis.

Author

Yuko Sato, Hilbert, Lennart, Haruka Oda, Yinan Wan, Heddleston, John M., Teng-Leong Chew, Zaburdaev, Vasily, Keller, Philipp, Lionnet, Timothee, Vastenhouw, Nadine, and Hiroshi Kimura

Subjects
HISTONE acetylation, RNA polymerase II, POST-translational modification, REGULATOR genes, CARRIER proteins
Abstract

Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with a-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms. [ABSTRACT FROM AUTHOR]

Published
2019
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41. A Moving Source of Matrix Components Is Essential for De Novo Basement Membrane Formation

Author

Matsubayashi, Yutaka, primary, Louani, Adam, additional, Dragu, Anca, additional, Sánchez-Sánchez, Besaiz J., additional, Serna-Morales, Eduardo, additional, Yolland, Lawrence, additional, Gyoergy, Attila, additional, Vizcay, Gema, additional, Fleck, Roland A., additional, Heddleston, John M., additional, Chew, Teng-Leong, additional, Siekhaus, Daria E., additional, and Stramer, Brian M., additional

Published
2017
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43. Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation

Author

Fritzsche, Marco, primary, Fernandes, Ricardo A., additional, Chang, Veronica T., additional, Colin-York, Huw, additional, Clausen, Mathias P., additional, Felce, James H., additional, Galiani, Silvia, additional, Erlenkämper, Christoph, additional, Santos, Ana M., additional, Heddleston, John M., additional, Pedroza-Pacheco, Isabela, additional, Waithe, Dominic, additional, de la Serna, Jorge Bernardino, additional, Lagerholm, B. Christoffer, additional, Liu, Tsung-li, additional, Chew, Teng-Leong, additional, Betzig, Eric, additional, Davis, Simon J., additional, and Eggeling, Christian, additional

Published
2017
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44. Protein-templated gold nanoparticle synthesis: protein organization, controlled gold sequestration, and unexpected reaction products

Author

Hart, Cassidy, primary, Abuladel, Nouf, additional, Bee, Madeleine, additional, Kreider, Megan C., additional, CVitan, Alexander C., additional, Esson, Moira M., additional, Farag, Andrew, additional, Ibeh, Trisha, additional, Kalivas, Eleni N., additional, Larco, Daniel-Mario, additional, Walker Long, Andrew, additional, Lymperopoulos, Loukas, additional, Mendel, Zachary, additional, Miles, Nancy, additional, Zareba, Carly M., additional, Schwabacher, James C., additional, Slucher, Helen, additional, Vinals, Javier, additional, Heddleston, John M., additional, Li, Wenyue, additional, Fox, Douglas M., additional, and Hartings, Matthew R., additional

Published
2017
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47. Detecting carbon in carbon: Exploiting differential charging to obtain information on the chemical identity and spatial location of carbon nanotube aggregates in composites by imaging X-ray photoelectron spectroscopy

Author

Gorham, Justin M., primary, Osborn, William A., additional, Woodcock, Jeremiah W., additional, Scott, Keana C.K., additional, Heddleston, John M., additional, Hight Walker, Angela R., additional, and Gilman, Jeffrey W., additional

Published
2016
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