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Summary: Electric syntrophy between fatty acid oxidizers and methanogens through direct interspecies electron transfer (DIET) is essential for balancing acidogenesis and methanogenesis in anaerobic digestion. Promoting DIET using electrically conductive additives proved effective in enhancing methanogenesis; however, its possibility to affect other microbial redox reactions in methanogenic systems has been little studied. This study provides the first confirmation of the electro-syntrophic coupling of sulfide oxidation to S0 with CO2-reducing methanogenesis in sulfur-rich methanogenic cultures supplemented with conductive magnetite (100–700-nm particle size). The H2S content in biogas, initially exceeding 5000 ppmv, decreased to below 1 ppmv along with an accumulation of extracellular S0 (60–70 mg/L; initially

This study experimentally investigates the effect of hydrogen addition on combustion and thermal characteristics of impinging non-premixed jet flames for low-heating values gases (LHVGs). We evaluate the flame morphology and stability using a concentric non-premixed combustor with an impingement plate. OH radicals are visualized using the OH* chemiluminescence and OH-planar laser-induced fluorescence (OH-PLIF) system. Emission characteristics are investigated by calculating CO and NOx emission indices. The results show that the flame stability region narrows as the heating value decreases but expands as hydrogen has been added. The low-OH radical intensity of LHVGs increases with the hydrogen addition. EICO and EINOx decrease with the reduction of heating values. EICO rapidly declines near the lifted flame limit due to the premixing of fuel and air downstream of the flame region. The effect of the hydrogen addition on EINOx is insignificant and shows very low emissions. The heat transfer rate into cooling water indicates a linear tendency with thermal power regardless of the fuel type. These findings show that LHVGs can be employed in existing-impinging flame systems so long as they remain within flame stability regions. Furthermore, hydrogen addition positively affects the expansion of flame stability, enhancing the utility of LHVGs.

BackgroundHealthcare workers have been identified as being at risk of occupational voice disorders. Among them, nurses working in intensive care units (ICUs) are particularly vulnerable due to the risk factors that are associated with their exposure to high levels of noise. Thus, this study aimed to determine the prevalence of voice disorders among ICU nurses.MethodsA questionnaire was administered to 100 ICU nurses from four hospitals in China. The questionnaire assessed vocal-related symptoms, perceived voice handicap, frequently heard noise sources, and the quality of communications.ResultsResults indicate that the most frequently reported voice symptoms were ‘voice tiredness’ and ‘voiceless’. Nurses working more than 50 h per week experienced voice symptoms more frequently than nurses working for 40–50 h per week. The median value of the perceived voice handicap score (VHI-30) was 23, indicating mild voice handicap, while 24% of the nurses reported severe voice handicap. Longer working hours and working at patient wards were significantly associated with higher VHI-30 scores. The nurses also reported that the quality of verbal communication with patients and colleagues and voice problems worsened during the COVID-19 pandemic.ConclusionMore than 20% of nurses reported severe voice handicap, however, voice handicap among ICU nurses did not appear universally to all nurses. Further research is necessary to identify the risk factors associated with voice disorders and the mechanism behind such heterogeneity among ICU nurses.

The combustion and thermal characteristics of impinging premixed flames for low heating value gases (LHVGs) composed of methane, propane, and nitrogen are experimentally investigated. For the experiment, a combustor with an impingement plate is used to study the flame stability regions based on flame shapes and visualize OH radical distribution. The OH radical distribution is observed via OH* chemiluminescence and OH planar laser-induced fluorescence (OH-PLIF). Thermal characteristics are investigated by measuring the temperature distribution of the plate and the heat transfer rate into the cooling water. The results show that the flame stability region narrows as the heating value of the fuels decreases. Also, as the equivalence ratio increases (i.e., »1), the flame stability region is expanded. The OH radical intensity of LHVGs is observed to be lower than that of methane. The OH-PLIF images show where OH radicals are more distributed in the flame. The temperature distribution of the plate is determined by the flame shape according to the heating value, and the heat transfer rate into the water has a linear tendency to the fuel's thermal power. Based on these findings, LHVGs can be utilized without system improvement within the flame stability range in the impinging flame system.

Cell adhesion is generally a prerequisite to the microbial bioleaching of sulfide minerals, and surface biofilm formation is modulated via quorum sensing (QS) communication. We explored the impact of the overexpression of endogenous QS machinery on the covellite bioleaching capabilities of Acidithiobacillus ferrooxidans, a representative acidophilic chemolithoautotrophic bacterium. Cells were engineered to overexpress the endogenous qs-I operon or just the afeI gene under control of the tac promoter. Both strains exhibited increased transcriptional gene expression of afeI and improved cell adhesion to covellite, including increased production of extracellular polymeric substances and increased biofilm formation. Under low iron conditions, the improved bioleaching of covellite was more evident when afeI was overexpressed alone as compared to the native operon. These observations demonstrate the potential for the genetic modulation of QS as a mechanism for increasing the bioleaching efficiency of covellite, and potentially other copper sulfide minerals.

Abstract Because it is important to develop new sustainable sources of edible protein, insects have been recommended as a new protein source. This study applied Life Cycle Assessment (LCA) to investigate the environmental impact of small-scale edible insect production unit in South Korea. IMPACT 2002 + was applied as the baseline impact assessment (IA) methodology. The CML-IA baseline, EDIP 2003, EDP 2013, ILCD 2011 Midpoint, and ReCiPe midpoint IA methodologies were also used for LCIA methodology sensitivity analysis. The protein, fat contents, and fatty acid profile of the investigated insect (Protaetia brevitarsis seulensis larvae) were analyzed to determine its potential food application. The results revealed that the studied edible insect production system has beneficial environmental effects on various impact categories (ICs), i.e., land occupation, mineral extraction, aquatic and terrestrial ecotoxicity, due to utilization of bio-waste to feed insects. This food production system can mitigate the negative environmental effects of those ICs, but has negative environmental impact on some other ICs such as global warming potential. By managing the consumption of various inputs, edible insects can become an environmentally efficient food production system for human nutrition.

Restricted interests (RIs) in autism spectrum disorder (ASD) are clinically impairing interests of unusual focus or intensity. They are a subtype of restricted and repetitive behaviors which are one of two diagnostic criteria for the disorder. Despite the near ubiquity of RIs in ASD, the neural basis for their development is not well understood. However, recent cognitive neuroscience findings from nonclinical samples and from individuals with ASD shed light on neural mechanisms that may explain the emergence of RIs. We propose the nexus model of RIs in ASD, a novel conceptualization of this symptom domain that suggests that RIs may reflect a co-opting of brain systems that typically serve to integrate complex attention, memory, semantic, and social communication functions during development. The nexus model of RIs hypothesizes that when social communicative development is compromised, brain functions typically located within the lateral surface of cortex may expand into social processing brain systems and alter cortical representations of various cognitive functions during development. These changes, in turn, promote the development of RIs as an alternative process mediated by these brain networks. The nexus model of RIs makes testable predictions about reciprocal relations between the impaired development of social communication and the emergence of RIs in ASD and suggests novel avenues for treatment development.

There are large uncertainties in the single-scattering (i.e., morphologies) and microphysical (i.e., concentrations) properties of ice particles whose size are less than ~100 µm. Insufficient resolutions of the most advanced cloud probes (e.g., cloud particle imager) cannot resolve the micrometer-scale morphologies of small ice particles. Further, the shattering of large ice particles on probes’ inlets or tips causes uncertainties in the measurement of the concentrations of small ice particles. These uncertainties have large impacts on the single-scattering and microphysical properties of small ice particles that are utilized to quantify the bulk radiative properties of cirrus. In this study, the impacts of uncertainties in the morphologies and concentrations of small ice particles on the bulk radiative properties of tropical cirrus were calculated using measurements acquired during the Tropical Warm Pool-International Cloud Experiment. Five different models (i.e., budding Buckyball, Chebyshev particle, droxtal, Gaussian random sphere, and sphere) that represent the shapes of small ice particles were used to calculate the single-scattering properties. The bulk radiative properties, average phase-function (P11¯), and average asymmetry parameter (g¯) were computed by combining the measured size/habit distributions and the calculated single-scattering properties of ice particles. The impacts of the selection of varying morphologies of small particles on the bulk radiative properties were quantified. For these calculations, the possible range of the concentrations of small ice particles which depend on the degree of shattered large particles were also used. The impacts of varying the single-scattering properties of small ice particles on the bulk radiative properties were the largest in the upper parts of cirrus (T < −60 °C), while they were the smallest in the lower parts of cirrus (−45 < T < −30 °C). The impacts of uncertainties in the concentrations of small ice particles on the bulk radiative properties were largest in the lower parts of cirrus (−45 < T < −30 °C), whereas they were smallest in the upper parts of cirrus (T < −60 °C). The effect of shattering was maximum in the lower parts of cirrus, whilst it was minimum in the upper parts of cirrus. The combined impacts of uncertainties in the single-scattering (i.e., morphologies) and microphysical (i.e., concentrations) properties of small ice particles revealed variations of up to 11.2% (127.1%; 67.3%) of the integrated intensity in the forward (sideward; backward) angles in P11¯ and a corresponding change in g¯ by up to 12.61%.

The consolidation of long-term memory for sensitization and synaptic facilitation in Aplysia requires synthesis of new mRNA including the immediate early gene Aplysia CCAAT enhancer-binding protein (ApC/EBP). After the rapid induction of ApC/EBP expression in response to repeated treatments of 5-hydroxytryptamine (5-HT), ApC/EBP mRNA is temporarily expressed in sensory neurons of sensory-to-motor synapses. However, the molecular mechanism underlying the rapid degradation of ApC/EBP transcript is not known. Here, we cloned an AU-rich element (ARE)-binding protein, ApAUFl, which functions as a destabilizing factor for ApC/EBP mRNA. ApAUFl was found to bind to the 3′ UTR of ApC/EBP mRNA that contains AREs and subsequently reduces the expression of ApC/EBP 3′ UTR-containing reporter genes. Moreover, overexpres-sion of ApAUFl inhibited the induction of ApC/EBP mRNA in sensory neurons and also impaired long-term facilitation of sensory-to-motor synapses by repetitive 5-HT treatments. These results provide evidence for a critical role of the posttranscriptional modification of ApC/EBP mRNA during the consolidation of synaptic plasticity. [ABSTRACT FROM AUTHOR]

Aplysia CCAAT enhancer-binding protein (ApC/EBP), a key molecular switch in 5-hydroxytryptamine (5-HT)-induced long-term facilitation of Aplysia, is quickly and transiently expressed in response to a 5-HT stimulus, but the mechanism underlying this dynamic expression profile remains obscure. Here, we report that the dynamic expression of ApC/EBP during long-term facilitation is regulated at the post-transcriptional level by AU-rich element (ARE)-binding proteins. We found that the 3′UTR of ApC/EBP mRNA contains putative sequences for ARE, which is a representative post-transcriptional cis-acting regulatory element that modulates the stability and/or the translatability of a distinct subset of labile mRNAs. We cloned the Aplysia homologue of embryonic lethal abnormal visual system homologue (ELAV/Hu) protein, one of the best-studied RNA-binding proteins that associate with ARE, and elucidated the involvement of Aplysia ELAV/Hu protein in ApC/EBP gene expressional regulation. Cloned Aplysia ELAV/Hu protein, Aplysia embryonic lethal abnormal visual system (ApELAV), bound to an AU-rich region within the 3′UTR of ApC/EBP mRNA. Additionally, ApELAV controlled the expression of ApC/EBP 3′UTR-containing reporter gene by functioning as a stability-enhancing factor. In particular, 5-HT-induced long-term facilitation was impaired when the AU-rich region within the 3′UTR of ApC/EBP was over-expressed, which suggests the significance of this region in 5-HT-induced ApC/EBP expression, and in the resultant formation of long-term facilitation. Our results imply that the Aplysia ARE-binding protein, ApELAV, can regulate ApC/EBP gene expression at the mRNA level, and accordingly, ARE-mediated post-transcriptional mechanism may serve a crucial function in regulating the expression of ApC/EBP in response to a 5-HT stimulus. [ABSTRACT FROM AUTHOR]

Abstract Background Noninvasive prenatal testing (NIPT) using massively parallel sequencing of cell-free DNA (cfDNA) is increasingly being used to predict fetal chromosomal abnormalities. However, concerns over erroneous predictions which occur while performing NIPT still exist in pregnant women at high risk for fetal aneuploidy. We performed the largest-scale clinical NIPT study in Korea to date to assess the risk of false negatives and false positives using next-generation sequencing. Methods A total of 447 pregnant women at high risk for fetal aneuploidy were enrolled at 12 hospitals in Korea. They underwent definitive diagnoses by full karyotyping by blind analysis and received aneuploidy screening at 11–22 weeks of gestation. Three steps were employed for cfDNA analyses. First, cfDNA was sequenced. Second, the effect of GC bias was corrected using normalization of samples as well as LOESS and linear regressions. Finally, statistical analysis was performed after selecting a set of reference samples optimally adapted to a test sample from the whole reference samples. We evaluated our approach by performing cfDNA testing to assess the risk of trisomies 13, 18, and 21 using the sets of extracted reference samples. Results The adaptive selection algorithm presented here was used to choose a more optimized reference sample, which was evaluated by the coefficient of variation (CV), demonstrated a lower CV and higher sensitivity than standard approaches. Our adaptive approach also showed that fetal aneuploidies could be detected correctly by clearly splitting the z scores obtained for positive and negative samples. Conclusions We show that our adaptive reference selection algorithm for optimizing trisomy detection showed improved reliability and will further support practitioners in reducing both false negative and positive results.

Humic substances (HSs) are chromogenic organic assemblies that are widespread in the environment, including soils, oceans, rivers, and coal-related resources. HSs are known to directly and indirectly stimulate plants based on their versatile organic structures. Their beneficial activities have led to the rapid market growth of agronomical HSs. However, there are still several technical issues and concerns to be addressed to advance sustainable agronomical practices for HSs and allow growers to use HSs reliably. First, it is necessary to elucidate the evident structure (component)–function relationship of HSs. Specifically, the core structural features of HSs corresponding to crop species, treatment method (i.e., soil, foliar, or immersion applications), and soil type-dependent plant stimulatory actions as well as specific plant responses (e.g., root genesis and stress resistance) should be detailed to identify practical crop treatment methodologies. These trials must then be accompanied by means to upgrade crop marketability to help the growers. Second, structural differences of HSs depending on extraction sources should be compared to develop quality control and assurance measures for agronomical uses of HSs. In particular, coal-related HSs obtainable in bulk amounts for large farmland applications should be structurally and functionally distinguishable from other natural HSs. The diversity of organic structures and components in coal-based HSs must thus be examined thoroughly to provide practical information to growers. Overall, there is a consensus amongst researchers that HSs have the potential to enhance soil quality and crop productivity, but appropriate research directions should be explored for growers’ needs and farmland applications.

Hybrid electric vehicles (HEVs) and plug-in hybrid electric vehicles (PHEVs) are evolving rapidly since the introduction of Toyota Prius into the market in 1997. As the world needs more fuel-efficient vehicles to mitigate climate change, the role of HEVs and PHEVs are becoming ever more important. While fuel economies of HEVs and PHEVs are superior to those of internal combustion engine (ICE) powered vehicles, they are partially powered by batteries and therefore they resemble characteristics of battery electric vehicles (BEVs) such as dependence of fuel economy on ambient temperatures. It is also important to understand how different extent of hybridization (a.k.a., hybridization ratio) affects fuel economy under various driving conditions. In addition, it is of interest to understand how HEVs and PHEVs compare with BEVs at a similar vehicle weight. This study investigated the relationship between vehicle mass and vehicle performance parameters, mainly fuel economy and driving range of PHEVs focused on 2018 and 2019 model years using the test data available from fuel economy website of the US Environmental Protection Agency (EPA). Previous studies relied on modeling to understand mass impact on fuel economy for HEV as there were not enough number of HEVs in the market to draw a trendline at the time. The study also investigated the effect of ambient temperature for HEVs and PHEVs and kinetic energy recovery of the regenerative braking using the vehicle testing data for model year 2013 and 2015 from Idaho National Lab (INL). The current study assesses current state-of-art for PHEVs. It also provides analysis of experimental results for validation of vehicle dynamic and other models for PHEVs and HEVs.

Air conditioner power consumption accounts for a large fraction of the total power used by hybrid and electric vehicles. This study examined the effects of three different cabin air ventilation settings on mobile air conditioner (MAC) power consumption, such as fresh mode with air conditioner on (ACF), fresh mode with air conditioner off (ACO), and air recirculation mode with air conditioner on (ACR). Tests were carried out for both indoor chassis dynamometer and on-road tests using a 2012 Toyota Prius plug-in hybrid electric vehicle. Real-time power consumption and fuel economy were calculated from On-Board Diagnostic-II (OBD-II) data and compared with results from the carbon balance method. MAC consumed 28.4% of the total vehicle power in ACR mode when tested with the Supplemental Federal Test Procedure (SFTP) SC03 driving cycle on the dynamometer, which was 6.1% less than in ACF mode. On the other hand, ACR and ACF mode did not show significant differences for the less aggressive on-road tests. This is likely due to the significantly lower driving loads experienced in the local driving route compared to the SC03 driving cycle. On-road and SC03 test results suggested that more aggressive driving tends to magnify the effects of the vehicle HVAC (heating, ventilation, and air conditioning) system settings. ACR conditions improved relative fuel economy (or vehicle energy efficiency) to that of ACO conditions by ~20% and ~8% compared to ACF conditions for SC03 and on-road tests, respectively. Furthermore, vehicle cabin air quality was measured and analyzed for the on-road tests. ACR conditions significantly reduced in-cabin particle concentrations, in terms of aerosol diffusion charger signal, by 92% compared to outside ambient conditions. These results indicate that cabin air recirculation is a promising method to improve vehicle fuel economy and improve cabin air quality.

This study investigated scaling trends of commercially available light-duty battery electric vehicles (BEVs) ranging from model year 2011 to 2018. The motivation of this study is to characterize the status of BEV technology with respect to BEV performance parameters to better understand the limitations and potentials of BEV. The raw data was extracted from three main sources: INL (Idaho National Laboratory) website, EPA (Environmental Protection Agency) Fuel Economy website, and the websites BEV manufacturers and internet in general. Excellent scaling trends were found between the EPA driving range per full charge of a battery and the battery capacity normalized by vehicle weight. In addition, a relatively strong correlation was found between EPA city fuel economy and vehicle curb weight, while a weak correlation was found between EPA highway fuel economy and vehicle curb weight. An inverse power correlation was found between 0⁻60 mph acceleration time and peak power output from battery divided by vehicle curb weight for 10 BEVs investigated at INL. Tests done on the environmentally controlled chamber chassis dynamometer at INL show that fuel economy drops by 19 ± 5% for the summer driving condition with air conditioner on and 47 ± 7% for the winter driving condition.