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Exposures to exogenous particles is of increasing concern to human health. Characterising the concentrations, chemical species, distribution, and involvement of the stimulus with the tissue microanatomy is essential in understanding the associated biological response. However, no single imaging technique can interrogate all these features at once which confounds and limits correlative analyses. Developments of synchronous imaging strategies, allowing multiple features to be identified simultaneously, is essential to assess spatial relationships between these key features with greater confidence. Here we present data to first highlight complications of correlative analysis between the tissue microanatomy and elemental composition associated with imaging serial tissue sections. This is achieved by assessing both the cellular and elemental distribution in 3-dimensional space using optical microscopy on serial sections and confocal X-ray fluorescence spectroscopy on bulk samples respectively. We propose a new imaging strategy using lanthanide tagged antibodies with X-ray fluorescence spectroscopy. Using simulations, a series of lanthanide tags were identified as candidate labels for scenarios where tissue sections are imaged. The feasibility and value of the proposed approach is shown where an exposure of Ti was identified concurrently with CD45 positive cells at sub-cellular resolutions. Significant heterogeneity in the distribution of exogenous particles and cells can be present between immediately adjacent serial sections showing clear need of synchronous imaging methods. The proposed approach enables elemental compositions to be correlated with the tissue microanatomy in a highly multiplexed and non-destructive manner at high spatial resolutions with the opportunity for subsequent guided analysis.

As a contribution towards a regional environmental context of human-climate interactions, the ICDP co-funded Chew Bahir Drilling Project, a part of the HSPDP (Hominin Sites and Paleolakes Drilling Project), recovered ~280-m long cores of sedimentary strata through continental scientific drilling in southern Ethiopia. The fluvio-lacustrine coring locality in the Chew Bahir basin is situated near key archaeological and paleoanthropological sites, such as the Omo-Kibish where the Omo 1 and 2 Homo sapiens fossils were recovered.Here we present the 620,000-year environmental record from Chew Bahir that provides an extraordinary opportunity to examine the potential influence of climate variability on hominin evolution, cultural innovation and dispersal during the Middle to Late Pleistocene. The near-continuous Chew Bahir record documents 13 environmental episodes that differ in length and character, potentially inducing habitat changes influencing hominin biological and cultural transformation. We infer that long-lasting and relatively stable humid conditions from ~620,000–275,000 years BP (Episodes 1–6) were interrupted by several abrupt and extreme hydroclimatic oscillations. This phase coincided with the appearance of high anatomical diversity in hominin groups. During Episodes 7–9 (~275,000–60,000 years BP), a pronounced pattern of climatic cyclicity was paralleled by the gradual transition from Acheulean to Middle Stone Age technologies, the emergence of H. sapiens in eastern Africa, and a key phase of human social and cultural innovation. Episodes 10–12 (~60,000–10,000 years BP), marked by high-frequency climate oscillations, is contemporaneous with the global dispersal of H. sapiens, facilitated by continued technological innovation and the alignment of humid pulses between eastern Africa and the eastern Mediterranean.Prospectively, the Chew Bahir record represents a crucial component for the Middle and Late Pleistocene in the ongoing efforts of the scientific community (future and upcoming ICDP-funded projects) to address questions in Africa across four topical core areas: paleoclimate, paleoenvironment, basin evolution, and modern lake systems.

In this study, we synthesize terrestrial and marine proxy records, spanning the past 620,000 years, to decipher pan-African climate variability and its drivers and potential linkages to hominin evolution. We find a tight correlation between moisture availability across Africa to El Niño Southern Ocean oscillation (ENSO) variability, a manifestation of the Walker Circulation, that was most likely driven by changes in Earth’s eccentricity. Our results demonstrate that low-latitude insolation was a prominent driver of pan-African climate change during the Middle to Late Pleistocene. We argue that these low-latitude climate processes governed the dispersion and evolution of vegetation as well as mammals in eastern and western Africa by increasing resource-rich and stable ecotonal settings thought to have been important to early modern humans.

Despite the vast array of different geochronological tools available, dating the Paleolithic remains one of the discipline’s greatest challenges. This review focuses on two different dating approaches: trapped charge and amino acid geochronology. While differing in their fundamental principles, both exploit time-dependent changes in signals found within crystals to generate a chronology for the material dated and hence, the associated deposits. Within each method, there is a diverse range of signals that can be analyzed, each covering different time ranges, applicable to different materials and suitable for different paleoenvironmental and archaeological contexts. This multiplicity of signals can at first sight appear confusing, but it is a fundamental strength of the techniques, allowing internal checks for consistency and providing more information than simply a chronology. For each technique, we present an overview of the basis for the time-dependent signals and the types of material that can be analyzed, with examples of their archaeological application, as well as their future potential.

Understanding eastern African paleoclimate is critical for contextualizing early human evolution, adaptation, and dispersal, yet Pleistocene climate of this region and its governing mechanisms remain poorly understood due to the lack of long, orbitally-resolved, terrestrial paleoclimate records. Here we present leaf wax hydrogen isotope records of rainfall from paleolake sediment cores from key time windows that resolve long-term trends, variations, and high-latitude effects on tropical African precipitation. Eastern African rainfall was dominantly controlled by variations in low-latitude summer insolation during most of the early and middle Pleistocene, with little evidence that glacial–interglacial cycles impacted rainfall until the late Pleistocene. We observe the influence of high-latitude-driven climate processes emerging from the last interglacial (Marine Isotope Stage 5) to the present, an interval when glacial–interglacial cycles were strong and insolation forcing was weak. Our results demonstrate a variable response of eastern African rainfall to low-latitude insolation forcing and high-latitude-driven climate change, likely related to the relative strengths of these forcings through time and a threshold in monsoon sensitivity. We observe little difference in mean rainfall between the early, middle, and late Pleistocene, which suggests that orbitally-driven climate variations likely played a more significant role than gradual change in the relationship between early humans and their environment.

Comprehensive mapping and the Briticechrono geochronology provides a reconstruction of the last advance and retreat of the only land-terminating ice lobe of the western British Irish Ice Sheet. The Irish Sea Glacier was fed by ice from Lake District, Irish Sea and Wales, and extended to maximum limits in the English Midlands. During ice retreat after 27 kyrs, a series of reverse bedrock slopes rendered proglacial lakes endemic in the land-system. Not resembling the more extensive definitions of the classical ‘Glacial Lake Lapworth’, these ice contact lakes were smaller time transgressive moraine- and bedrock-dammed basins that evolved with ice marginal retreat. Combining, for the first time on glacial sediments, OSL bleaching profiles for cobbles with single grain and small aliquot OSL measurements on sands, has produced a coherent chronology from these heterogeneously bleached samples, and constrained for the Irish Sea Glacier a post 30ka ice maximum advance, 26.5±1.8ka maximum extent, and 25.3±1.6 to 20.6±2.2ka retreat vacating the region. With retreat of the Irish Sea Glacier an opportunistic Welsh re-advance 19.7±2.5ka took advantage of the vacated space and rode over Irish Sea Glacier moraines. Our geomorphological chronosequence shows a glacial system forced by climate, but mediated by piracy of ice sources shared with the larger and marine terminating Irish Sea Ice Stream to the west. The Irish Sea Glacier underwent changes flow regime and fronting environments driven by stagnation and decline as the primary impetus to advance was diverted. Ultimately, the glacier of the English Midlands display complex uncoupling and realignment during deglaciation and ice margin retreat towards upland hinterlands ~17.8 kyrs (Lake District and Pennines) and asynchronous behaviour as individual adjacent ice lobes became increasingly important in driving the landform record.

Reconstructions of climatic and environmental conditions can contribute to current debates about the factors that influenced early human dispersal within and beyond Africa. Here we analyse a 200,000-year multi-proxy paleoclimate record from Chew Bahir, a tectonic lake basin in the southern Ethiopian rift. Our record reveals two modes of climate change, both associated temporally and regionally with a specific type of human behavior. The first is a long-term trend towards greater aridity between 200,000 and 60,000 years ago, modulated by precession-driven wet-dry cycles. Here, more favorable wetter environmental conditions may have facilitated long-range human expansion into new territory, while less favorable dry periods may have led to spatial constriction and isolation of local human populations. The second mode of climate change observed since 60,000 years ago mimics millennial to centennial-scale Dansgaard-Oeschger cycles and Heinrich events. We hypothesize that human populations may have responded to these shorter climate fluctuations with local dispersal between montane and lowland habitats.

In this study, we synthesize terrestrial and marine proxy records, spanning the past 620 ky, to decipher pan-African climate variability and its drivers and potential linkages to hominin evolution. We find a tight correlation between moisture availability across Africa to El Niño Southern Ocean oscillation (ENSO) variability, a manifestation of the Walker Circulation, that was most likely driven by changes in Earth's eccentricity. Our results demonstrate that low-latitude insolation was a prominent driver of pan-African climate change during the Middle to Late Pleistocene. We argue that these low-latitude climate processes governed the dispersion and evolution of vegetation as well as mammals in eastern and western Africa by increasing resource-rich and stable ecotonal settings thought to have been important to early modern humans.

In this study we synthesize terrestrial and marine proxy records spanning the last 620,000 years, to decipher pan-African climate variability, its drivers and potential linkages to hominin evolution. We find a tight correlation between moisture availability across Africa to Walker and Hadley Circulation variability that were most likely driven by changes in Earth´s eccentricity. Our results demonstrate that low latitude insolation rather than glacial-interglacial cyclicity was the predominant driver of pan-African climate change during the mid to late Pleistocene. We argue that these low-latitude climate processes governed the dispersion and evolution of vegetation, as well as mammals, in eastern and western Africa, ultimately by increasing resource-rich and stable ecotonal settings that have long thought to have been the preferred habitats of hominins.

Alkali feldspar is routinely used in retrospective dosimetry using luminescence methods. However there is a signal loss over time, termed ‘anomalous fading’, which results in age underestimation if uncorrected. Although significant improvements have been made in recent years, luminescence dating of feldspars remains challenging. This paper investigates the relationships between chemistry, structural state and the scale of exsolution with thermoluminescence (TL) emission spectra and infrared stimulated luminescence (IRSL) fading rates. We measure TL emission spectra, where possible linking the recombination site to physical features of the feldspar crystals. We show that fading rates are lowest in ordered end-member Na- and K-feldspars but significantly greater in disordered end-members, showing that Al–Si order influences fading. As well as having very low fading rates, ordered end-member samples have distinctive TL emission spectra, with the yellow-green emission dominant, while all other samples have a dominant blue emission. Perthite, i.e. exsolved members of the (Na,K)-feldspar solid solution, show greater fading than disordered end-members and fading is greatest in semi-coherent macroperthite. We propose that the state of Al–Si-order, and the occurrence of defects and dislocations at the perthite lamellar interfaces influence anomalous fading rates in feldspar.

Gingivitis is an extremely common oral inflammatory condition and can be induced in humans using an acute 21-day experimental gingivitis model. Neutrophils are known to be highly prevalent in the gingival crevice during gingival inflammation; however, the effect of gingivitis and the associated biofilm on peripheral blood neutrophils (PBN) is not well characterised. Thus, the aim of this study was to examine the impact of inflammation induced by experimental gingivitis and its resolution upon the function of PBN. Fifteen systemically healthy volunteers undertook a split-mouth 21-day experimental gingivitis study followed by a resolution phase of 14 days. PBN function, including reactive oxygen species (ROS) production, neutrophil extracellular trap (NET) release, directional chemotactic accuracy and expression of host mediators in gingival crevicular fluid (GCF), were measured at baseline (day 0), on day 21 and on day 35. NET formation and ROS production were significantly elevated at day 21. Chemotactic speed was also elevated in response to bacterial peptide fMLP at day 21. At day 35, ROS production in response to an Fcgamma stimulant, opsonised Staphylococcus aureus, remained elevated. The data presented suggest a lasting biological impact of the experimental gingivitis on PBN function even after clinical symptoms have abated.

Feldspars are ubiquitous natural dosimeters widely used in luminescence dating. Despite decades of research, the lattice defects as well as the mechanisms involved in luminescence production in feldspars remain poorly understood. The recently developed method of infrared photoluminescence (IRPL) excitation-emission spectroscopy has revealed the presence of two electron trapping centres emitting IRPL at 1.3 eV and 1.41 eV (IRPL1.3 and IRPL1.41 centres), and it enables detailed investigations into the ground and excited state energies of these centres. Here we make measurements of a range of single crystal alkali feldspars to understand the effects of feldspar chemical composition, crystal structure and framework disorder on the physical characteristics of IRPL1.3 and IRPL1.41 electron trapping centres. Measurements of our sample suite reveals IRPL and IR-radioluminescence (RL) emissions at 1.41 eV, 1.3 eV and, for the first time, at 1.2 eV. Our results show that whilst the IRPL1.3 trapping centre is unaffected by the M site cation occupancy, the presence of IRPL1.41 trapping centres seems to be linked to the presence of K+ ions on M sites. However, no clear trends in IRPL and IR-RL emission energies and signal intensities with chemical composition of the samples were found. Exploring the effect of framework disorder on IRPL1.3 and IRPL1.41 emissions revealed no significant changes to IRPL and IR-RL emission energies or ground state energies of the trapping centres, suggesting that the corresponding defects are not located on bridging O ions. Variations in ground state energies across the whole sample suite range from 2.04 eV to 2.20 eV for the IRPL1.3 centre and from 2.16 eV to 2.46 eV for the IRPL1.41 centre. Variations in trap depth seem to be driven by other factors than sample chemistry, degree of Al3+disorder and number of phases present in a single crystal feldspar. Interestingly, the IR resonance peak is invariant between samples. Regarding the use of IRPL in luminescence dating, we show that optical resetting differs for the three different emissions, with the emission at ∼1.41 eV not being reset in some samples even after 18 h of solar bleaching 2021 IOP Publishing Ltd.

International audience; The BRITICE-CHRONO Project has generated a suite of recently published radiocarbon ages from deglacial sequences offshore in the Celtic and Irish seas and terrestrial cosmogenic nuclide and optically stimulated luminescence ages from adjacent onshore sites. All published data are integrated here with new geochronological data from Wales in a revised Bayesian analysis that enables reconstruction of ice retreat dynamics across the basin. Patterns and changes in the pace of deglaciation are conditioned more by topographic constraints and internal ice dynamics than by external controls. The data indicate a major but rapid and very short-lived extensive thin ice advance of the Irish Sea Ice Stream (ISIS) more than 300 km south of St George's Channel to a marine calving margin at the shelf break at 25.5 ka; this may have been preceded by extensive ice accumulation plugging the constriction of St George's Channel. The release event between 25 and 26 ka is interpreted to have stimulated fast ice streaming and diverted ice to the west in the northern Irish Sea into the main axis of the marine ISIS away from terrestrial ice terminating in the English Midlands, a process initiating ice stagnation and the formation of an extensive dead ice landscape in the Midlands.

Humans have been adapting to more demanding habitats in the course of their evolutionary history. Nevertheless, environmental changes coupled with overpopulation naturally limit competition for resources. In order to find such limits, reconstructions of climate and population changes are increasingly used for the continent of our origin, Africa. However, continuous and high-resolution records of climate-human interactions are still scarce. Using a 280 m sediment core from Chew Bahir*, a wide tectonic basin in southern Ethiopia, we reconstruct the paleoenvironmental conditions during the development of Homo sapiens. The complete multiproxy record of the composite core covers the last ~600 ka , allowing tests of hypotheses about the influence of climate change on human evolution and technological innovation from the Late Acheulean to the Middle/Late Stone Age, and on dispersal within and out of Africa. Here we present results from the uppermost 100 meters of the Chew Bahir core, spanning the last 200 kiloyears (ka). The record shows two modes of environmental change that are associated with two types of human mobility. The first mode is a long-term trend towards a more arid climate, overlain by precession-driven wet-dry alternation. Through comparison with the archaeological record, humid episodes appear to have led to the opening of ‘green’ networks between favourable habitats and thus to increased human mobility on a regional scale. The second mode of environmental change resembles millennial-scale Dansgaard-Oeschger and Heinrich events, which seem to coincide with enhanced vertical mobility from the Ethiopian rift to the highlands, especially in the time frame between ~65–21 ka BP. The coincidence of climate change and human mobility patterns help to define the limiting conditions for early Homo sapiens in eastern Africa.___________________* cored in the context of HSPDP (Hominin Sites and Paleolakes Drilling Project) and CRC (Collaborative Research Centre) 806 “Our way to Europe”

What role did climate dynamics play in the evolution and dispersal of Homo sapiens within and beyond Africa, and in key cultural innovations? Were gradual climatic changes, rapid shifts from wet to dry, or short-term climate flickers the main driver of human evolution and migration? As a contribution towards an enhanced understanding of those possible human-climate interactions the Chew Bahir Drilling Project, part of the Hominin Sites and Paleolakes Drilling Project (HSPDP) and the Collaborative Research Center (CRC) 806 “Our way to Europe”, recovered two ~280 m-long sediment cores from a deep, tectonically-bound basin in the southern Ethiopian rift in late 2014. The Chew Bahir record covers the past ~600 ka of environmental history, a critical time period that includes the transition from the Acheulean to the Middle Stone Age, and the origin and dispersal of Homo sapiens. Here we present the results from our multi-proxy study of the Chew Bahir 280 m-long composite core, providing a detailed and high-resolution record of eastern Africa’s climate oscillations during the last ~600 ka. To determine sediment age we used a Bayesian model to combine ages derived from radiocarbon dating of ostracodes, optically-stimulated luminescence (OSL) dating of quartz, Argon-Argon (40Ar/39Ar) dating of feldspar grains from some key (micro)tephra layers, and correlation on the basis of geochemistry of a tephra unit in the core to a known and dated tephra in the outcrop. We used high-resolution geophysical and geochemical indicators, such as the established aridity proxy K, sediment colour and authigenic minerals to differentiate between climate fluctuations on different time scales and magnitudes. Our results show that the full proxy record from Chew Bahir can be divided into three phases with similar trends in central tendency and dispersion. Phase I from ~600 to ~430 kyr BP shows a long-term shift from humid to arid conditions while slightly increasing the variability and ending with the most extreme oscillations between full humidity and extreme aridity. The transition into Phase II (~430 to ~200 kyr BP) is marked by a pronounced millennial-scale humidity increase. Phase II reflects generally more humid conditions and there is evidence of double humidity increase tendency. Firstly, between ~430 and ~315 kyr BP (Phase IIa), and again from ~280 to ~195 kyr BP (Phase IIb), with only slight changes in long-term variability. Since ~200 kyr BP (Phase III), a long-term aridification trend sets in, similar to Phase I, but with a distinct increase in variability and amplitudes. All of these changes would have had significant implications for shaping our ancestors’ living environments, both broadening and limiting their options in response to the different degrees and rates of climatic stress. The Chew Bahir record, one of the very few long terrestrial environmental records from continental eastern Africa, can contribute to testing the influence of low versus high latitude climate change in driving the expansion, contraction and fragmentation of early human habitats.

The sediment cores of the Chew Bahir drilling project, part of the Hominin Sites and Paleolakes Drilling Project (HSPDP), from southern Ethiopia, were used to reconstruct climatic changes by analyzing the sediment geochemistry with high-resolution XRF scanning. To interpret the multidimensional XRF dataset we computed a principal component analysis. We used the first principal component (PC1) to detect changes in variability by running a windowed standard deviation analysis and additionally a change point analysis to detect the exact timing of variability changes. Additionally we used the established Chew Bahir log(K/Zr) aridity proxy, representing clay mineral chemistry- detrital input ratio and compared it to a new Chew Bahir climate indicator, the log(Ca/Ti) proxy, an evaporation signal that is probably inversely related to lake level stands. We find that the log(Ca/Ti) record is also an exceptionally good climate indicator because, compared to the established log(K/Zr) proxy, it reacts with greater amplitude to insolation-controlled signals such as orbital precession. This is confirmed by the log (Ca/Ti) record showing a very clear signal during the African Humid Period, which is however less pronounced in the log(K/Zr) record. To gain a deeper understanding of the climate cycles and their temporal evolution, we computed a continuous wavelet transformation (CWT) for each of the climate proxies, and studied temporal changes in their cyclicity. Our results indicate that in addition to the precession cycle (~ 20 kyr), the Chew Bahir climate record contains earth eccentricity cycles (~ 100 kyr), as well as half-precession cycles during high eccentricity. During low eccentricity (450-350 kyr ago), we find reduced variability, three of five changes in standard deviation, damped precession and half precession cycles, and an abrupt transition from dry to wet climate, possibly due to climatic change in high latitudes which may be related to the Mid-Bruhnes event (MBE). The results confirm that during high eccentricity the tropics are insolation controlled, largely independent of the high latitudes, whereas during low eccentricity the climate of tropical eastern Africa is sensitive to climatic drivers other than precession, possibly originating from high latitudes. Such a period occurring 450 to 350 kyr ago could have led to large regional differences in moisture availability and may have affected early humans by habitat separation, which by isolating populations, resulted in technological diversification. This possible scenario may help to explain the technological transition from Middle Stone Age (MSA) to Acheulean technology that was documented in the Olorgesailie basin during the same time period.

The recent introduction of post-IR IRSL measurement protocols has prompted a resurgence in luminescence applications using feldspar, some of which are affected by anomalous fading related signal loss. Many fading-corrected feldspar ages are reported in the literature, however few of those ages have been corrected using the model of Huntley (2006) [Huntley, D.J., 2006. An explanation of the power-law decay of luminescence. Journal of Physics: Condensed Matter 18(4), 1359–1365]. Here we present a new R function that calculates fading-corrected ages using the model of Huntley (2006), implemented with either a single-saturating exponential (1EXP) or general-order kinetic (GOK) fit. We evaluate the performance of the model through (i) contrasting measured and modelled field saturation values for a suite of 41 published saturated samples, and (ii) through using the model to fading-correct feldspar ages of samples with independent age control. Our results indicate that when implemented with 1EXP this model has an accuracy of 10% for predicting sample saturation, but that independent ages may be overestimated when the model is used to fading-correct samples across a range of timescales. In contrast, providing that the dose response curve has been characterised beyond 600 Gy, implementing the Huntley (2006) model with a GOK fit yields accurate age estimations. Modelled age overestimation for 1EXP is associated with dose response curve deviation from a single-saturating exponential. Finally we contrast the laboratory measured light levels of a suite of 50 saturated samples with their corresponding fading rates. We show that these saturated samples may yield De values below 2D0, and thus that 2D0 is not an effective screening criterion for sample saturation where no anomalous fading correction is made.

Basic assumptions of the single aliquot regenerative dose (SAR) protocol are tested using the violet stimulated luminescence (VSL) signal from quartz. The VSL signal is shown to be reduced to a sufficiently low background level between SAR steps, and the SAR protocol appears to adequately correct for sensitivity changes during measurement. The VSL SAR protocol can recover a large (405 Gy) laboratory beta dose within uncertainties, however the mean value for the dose recovery ratio is commonly 0.8 or less. This poor behaviour is echoed in the measurements of equivalent dose (De) for a sample with an expected De of ∼354 Gy, which underestimates De by 50–70%. Further investigations are required to understand the mechanisms underlying these underestimations in VSL SAR De values.

Sets of sandy beach ridges and intervening swales define shoreline sections of the shallow St Lucia wetland system within the iSimangaliso Wetland Park, World Heritage Site in northern KwaZulu-Natal province, South Africa. The sets comprise 3–10 beach ridges, the most prominent being 80–150 m wide and rising 0.5–2 m above the adjacent swales. The highest beach ridge crests elevated 3.2–4.6 m above mean sea level are furthest from the present shoreline and the lowest ridges, rising about 0.5 m above the swales, occur closest to the present mean lake level shoreline. This investigation assesses the genesis of the sandy beach ridges on five strand plain remnants within the estuarine lake. The ridges were topographically surveyed and their ages estimated using optically stimulated luminescence (OSL) dating. OSL dating reveals that the oldest beach ridge formed around 6240 years ago, with a sequence of beach ridges having accumulated during the period ~4000 to 1500 years ago reflecting coeval accretion around the lake. The present mean lake level shoreline was only reached within the past ~600 years. Sedimentation changed from marine-dominated to lacustrine deposition in the estuary during the period of beach ridge accretion. The dated beach ridges, supported by new radiocarbon dates of fixed biological indicators from Holocene intertidal zone settings on the coast, are used with published sea-level curves to set the context of periodic beach ridge accretion in the marine-linked estuarine lake. The dated ridges suggest that episodic regression of the estuarine lake shoreline occurred after a mid-Holocene sea-level highstand. The record of sea-level change is also reflected by the long-term natural shrinking and shallowing of the proto-St Lucia lagoon/estuarine lake in the context of reduced marine influences due to closure of former marine channels by progressive barrier dune accretion. The sequence of coeval beach ridges reflects a pulsed lowering of relative sea-level in the shallowing estuarine lake. Height differences between the coeval ridges at sites around the lake reflect local environmental controls including the range of wind fetch distances across shallow lake compartments, wave height and wind-induced seiche effects.

Changes in East Asian summer and winter monsoon intensity have played a pivotal role in the prosperity and decline of society in the past, and will be important for future climate scenarios. However, the phasing of changes in the intensity of East Asian summer and winter monsoons on millennial and centennial timescales during the Holocene is unclear, limiting our ability to understand the factors driving past and future changes in the monsoon system. Here, we present a high resolution (up to multidecadal) loess record for the last 3.3 ka from the southern Chinese Loess Plateau that clearly demonstrates the relationship between changes in the intensity of the East Asian summer and winter monsoons, particularly at multicentennial scales. At multimillennial scales, the East Asian summer monsoon shows a steady weakening, while the East Asian winter monsoon intensifies continuously. At multicentennial scales, a prominent ∼700–800 yr cycle in the East Asian summer and winter monsoon intensity is observed, and here too the two monsoons are anti-phase. We conclude that multimillennial changes are driven by Northern Hemisphere summer insolation, while multicentennial changes can be correlated with solar activity and changing strength of the Atlantic meridional overturning circulation.

The Shirasuka lowlands, located on the Enshu-nada coast of central Japan, record evidence for numerous extreme wave events. Here we test the applicability of using the luminescence signal from feldspars to date these young (130 procedure. We demonstrate that this IRSL50 (pre-IR130) signal results in reliable ages when corrected for fading, and the post-IR IRSL130 stimulation functions as an optical wash for both the natural/regenerated luminescence signal (Lx) and the test dose signal (Tx), lowering the recuperation and removing any remaining charge from previous steps in the protocol. The single grain IRSL50 (pre-IR130) ages generated cover the historical record of the past 800 years and correlate well with past earthquakes and tsunamis in 1361 CE, 1498 CE and 1605 CE. Another identified tsunami deposit may correlate with the 1707 CE earthquake and tsunami. A slope failure deposit, probably caused by the earthquake in 1944 CE, is also identified. This study demonstrates that accurate ages can be determined for the young, extreme wave events at this site using the luminescence signal from feldspars.

A series of dose recovery experiments are undertaken on grains of potassium-rich feldspar using a single aliquot regenerative dose (SAR) protocol, measuring the post-infrared infrared stimulated luminescence (post-IR IRSL) signal. The ability to successfully recover a laboratory dose depends upon the size of the test dose used. It is shown that using current SAR protocols, the magnitude of the luminescence response (Tx) to the test dose is dependent upon the size of the luminescence signal (Lx) from the prior regeneration dose because the post-IR IRSL signal is not reduced to a low level at the end of measuring Lx. Charge originating from the regeneration dose is carried over into measurement of Tx. When the test dose is small (i.e. 1%–15% of the given dose) this carry-over of charge dominates the signal arising from the test dose. In such situations, Tx is not an accurate measure of sensitivity change. Unfortunately, because the carry-over of charge is so tightly coupled to the size of the signal arising from the regeneration dose, standard tests such as recycling will not identify this failure of the sensitivity correction. The carry-over of charge is due to the difficulty of removing the post-IR IRSL signal from feldspars during measurement, and is in stark contrast with the fast component of the optically stimulated luminescence (OSL) signal from quartz for which the SAR protocol was originally designed.

Climatic change is widely acknowledged to have played a role in the dispersal of modern humans out of Africa, but the timing is contentious. Genetic evidence links dispersal to climatic change ~60,000 years ago, despite increasing evidence for earlier modern human presence in Asia. We report a deep seismic and near-continuous core record of the last 150,000 years from Lake Tana, Ethiopia, close to early modern human fossil sites and to postulated dispersal routes. The record shows varied climate towards the end of the penultimate glacial, followed by an abrupt change to relatively stable moist climate during the last interglacial. These conditions could have favoured selection for behavioural versatility, population growth and range expansion, supporting models of early, multiple dispersals of modern humans from Africa.

This study compares natural and laboratory generated thermally transferred optically stimulated luminescence (TT-OSL) dose response curves (DRCs) for fine-grain quartz extracts from the Luochuan loess section in central China. Both DRCs saturate at high doses relative to the quartz OSL signal; the natural TT-OSL DRC saturates at about 2200 Gy and laboratory DRCs saturate at about 2700 Gy. However, the natural and laboratory TT-OSL DRCs deviate from one another at circa 150 Gy resulting in TT-OSL equivalent dose underestimation relative to palaeodoses expected from dose rates and independent age control. The lifetime of the TT-OSL signal at 10 °C, calculated from values of trap parameters E and s, is compared against the value for lifetime of the TT-OSL signal in nature at average burial temperature as determined from the age underestimation caused by deviation of the natural and laboratory generated DRCs. These two independent assessments of TT-OSL signal lifetime at Luochuan give similar values, suggesting that laboratory measurements of thermal stability reflect natural burial lifetimes and can potentially be used to correct TT-OSL ages for the difference between natural and laboratory dose response curves.

The evolution of Holocene moisture in semiarid and arid regions of Central Asia is key to understanding changes in current and future water resources, and has been a hotly debated topic in the past few decades. Central Asian loess deposits have shown potential as records of past moisture changes, particularly during the Holocene. Here, the variations in effective moisture during the Holocene in the semiarid regions of the Yili Basin, Central Asia, are obtained from three loess sections. Reliable chronologies were constructed using 26 quartz optically stimulated luminescence (OSL) ages. The strengthening pedogenesis, steady increases in magnetic susceptibility, and continuous decreases in mean grain size in the three loess sections all suggest that effective moisture continuously increased throughout the Holocene, indicating a dry early Holocene, a moderately humid middle Holocene, and a wet late Holocene. Rainfall carried by the Asian summer monsoon and the Westerlies contributes to the moisture changes in Central Asia. However, considering the dominance of evaporation over rainfall in controlling aridity-humidity shifts in this region, the continuous weakening of evaporation caused by a decrease in Northern Hemisphere summer insolation and associated atmospheric temperatures is primarily responsible for the wetting trend throughout the Holocene in the semiarid regions of the Yili Basin.

A suite of 10 samples collected from an 11 m thick colluvial sequence at Voordrag, KwaZulu-Natal, South Africa, have been used to undertake a comparison of different luminescence methods. Good agreement is found between single grain quartz optically stimulated luminescence (OSL) and single grain K-feldspar post-infrared infrared-stimulated luminescence (post-IR IRSL) ages, with the exception of the basal samples where the quartz OSL signal is saturated. Multiple grain quartz OSL consistently yields ages older than single grain OSL methods. Multiple grain feldspar ages derived from the IRSL50 signal are underestimated due to anomalous fading. A previously published radiocarbon chronology yields ages that are younger than those from single grain quartz OSL and post-IR IRSL, and this is most likely due to contamination with younger carbon. Identifying the effect of saturation on the quartz OSL signal remains challenging when quartz is dated in isolation. However, using a paired quartz/feldspar dating approach is an effective way of identifying the impact of saturation on the OSL signal.

Existing infrared photoluminescence (IRPL) systems have used pulsed infrared stimulation (~830 nm) and measured IRPL emission (at 880 or 955 nm) using time resolved data collection with photomultipliers. Breakthrough of the infrared stimulation light overwhelms the IRPL, but the delayed emission during the laser-off period has been used instead. This paper describes a system for measurement of the IRPL signal from single sand-sized grains of feldspar. The attachment uses an electron-multiplying charge-coupled device (EMCCD) imaging system, and has two innovations that make it possible to use such a detector to obtain IRPL data. First, the optical detection system has been designed to minimise stray light and maximise the efficiency with which filters reject the stimulation light. This acts to reduce, but not eliminate, the breakthrough. Second, by placing the sample to be measured in a clearly defined sample grid, the spatial resolution provided by the EMCCD has been used to differentiate between regions of the image where IRPL is emitted and adjacent regions where only breakthrough is expected. This allows quantification of the breakthrough and effective subtraction to isolate the IRPL signal from the grains of interest. The attachment has been used to measure IRPL from single sand-sized grains of feldspar from an aeolian dune from New Zealand. A 1W UV LED (365 nm) is also added to the system and this is effective at resetting the IRPL signal, permitting a single aliquot regenerative dose (SAR) protocol to be used to measure equivalent dose (De). Measurement of a known laboratory dose (104 Gy) demonstrates the reproducibility of the attachment, with no overdispersion observed in the resulting single grain De values. The recovered dose is within 10% of the given dose. The natural IRPL signal yields De values from single grains with low overdispersion (22%) and giving a weighted mean value (103 ± 5.8 Gy) that is consistent with that obtained using post-IR IRSL measurements (105 ± 3.8 Gy). The attachment described here provides IRPL measurements on single grains suitable for exploring the potential of this novel and exciting signal for dating geological sediments.

Climatic change is widely acknowledged to have played a role in the dispersal of modern humans out of Africa, but the timing is contentious. Genetic evidence links dispersal to climatic change ~60,000 years ago, despite increasing evidence for earlier modern human presence in Asia. We report a deep seismic and near-continuous core record of the last 150,000 years from Lake Tana, Ethiopia, close to early modern human fossil sites and to postulated dispersal routes. The record shows varied climate towards the end of the penultimate glacial, followed by an abrupt change to relatively stable moist climate during the last interglacial. These conditions could have favoured selection for behavioural versatility, population growth and range expansion, supporting models of early, multiple dispersals of modern humans from Africa.

This work was supported by the UK Space Agency CREST3 program under grant ST/P001998/1. Research in Next Generation Luminescence methods in Aberystwyth is supported by NERC grant CC003, and by HEFCW infrastructure funding for SPARCL. In recent years a number of portable instruments have been built for measuring the optically stimulated luminescence (OSL) signal from naturally occurring minerals. Some of these instruments have incorporated ionising radiation sources, giving the possibility of determining an equivalent dose (De), but little use has been made of these. One challenge has been that heating samples in this type of equipment is a major engineering challenge, yet methods for De determination use thermal pretreatments to remove charge from unstable traps, making signals arising from irradiation in nature and the laboratory comparable. This paper explores three strategies for obtaining accurate estimates of the De of samples in situations where thermal treatments are not possible: (1) deriving a correction factor based on comparing De values obtained using protocols with and without heating; (2) removing the contribution from the 110 °C TL peak and other unstable defects by component fitting the unheated OSL signal; and (3) adding a small beta dose to the sample prior to measurement of the natural luminescence signal so that the 110 °C TL peak is filled, making this measurement comparable with regeneration measurements where this peak is also populated. All three methods are promising when applied to quartz that has been physically separated from samples using standard laboratory procedures. The next step in this work will be to explore whether such methods can be applied to mixed mineral assemblages as would be encountered in the field. Postprint

The numerous dispersal events that have occurred during the prehistory of hominin lineages are the subject of longstanding and increasingly active debate in evolutionary anthropology. As well as research into the dating and geographic extent of such dispersals, there is an increasing focus on the factors that may have been responsible for dispersal. The growing body of detailed regional palaeoclimatic data is invaluable in demonstrating the often close relationship between changes in prehistoric environments and the movements of hominin populations. The scenarios constructed from such data are often overly simplistic, however, concentrating on the dynamics of cyclical contraction and expansion during severe and ameliorated conditions respectively. This contribution proposes a two-stage hypothesis of hominin dispersal in which populations (1) accumulate high levels of climatic tolerance during highly variable climatic phases, and (2) express such heightened tolerance via dispersal in subsequent low-variability phases. Likely dispersal phases are thus proposed to occur during stable climatic phases that immediately follow phases of high climatic variability. Employing high resolution palaeoclimatic data from Lake Tana, Ethiopia, the hypothesis is examined in relation to the early dispersal of Homo sapiens out of East Africa and into the Levant. A dispersal phase is identified in the Lake Tana record between c. 112,550 and c. 96,975 years ago, a date bracket that accords well with the dating evidence for H. sapiens occupation at the sites of Qafzeh and Skhul. Results are discussed in relation to the complex pattern of H. sapiens dispersal out of East Africa, with particular attention paid to the implications of recent genetic chronologies for the origin of non-African modern humans.

In recent years opercula of the snail species Bithynia tentaculata have been shown to emit thermoluminescence (TL) signals that can be used to determine equivalent dose, and may be capable of dating events throughout the entire Quaternary period. Concentric growth lines are a notable feature of almost all B. tentaculata opercula, but it is not known whether the luminescence emitted by the opercula is influenced by these structures. This study uses a newly developed EMCCD imaging system to measure the TL signals from opercula. A combination of microscopic analysis of the opercula using visible imagery, and measurement of the TL using the EMCCD system has been undertaken. Variations in TL intensity and equivalent dose (De) are seen, but the two are not correlated. Changes in TL intensity broadly mimic the concentric growth structures, but the largest variations in intensity are between different margins of the opercula, not individual growth bands. The EMCCD system makes it possible to produce a two dimensional map of the De measured from an operculum. Dose recovery experiments give De values that are consistent with each other across the whole opercula. Measurement of the De arising from irradiation in nature shows significant variability across a single operculum, but at present the reason for this variability is unknown.

The Qinghai-Tibetan Plateau is an important area for the study of Quaternary glaciation. Optically stimulated luminescence (OSL) dating has the potential to contribute to the chronology of glaciation in this region, but it is important to assess the accuracy of OSL dating of these glacial sediments. In this study, single grain quartz OSL signals are examined for five glacial samples collected from the moraines outside the Baiyu Valley, southeastern Tibet. The quartz grains exhibit poor luminescence characteristics, with a small proportion of grains passing the screening criteria. Grains which pass the screening criteria have relatively low signal intensity, leading to De values with large uncertainties. MAM and CAM were used to determine De values for these samples. The OSL ages are consistent with the sequence of events derived from the geomorphological relationship of the samples, and also with previous published radiocarbon ages. However, it is more difficult to reconcile the OSL ages and the terrestrial cosmogenic nuclide (TCN) 10Be ages. Analysis of both single grain quartz OSL data and TCN 10Be data is complex in this area. Further work is required to increase confidence in the OSL ages generated for the glacial sediments from this region.

Thermal pretreatments are used prior to optically stimulated luminescence (OSL) measurements in methods of dose determination, but the reproducibility of such heating appears to be poor within laboratories, and between laboratories. Monitoring the position of the 110 °C thermoluminescence (TL) peak in quartz can be used to routinely assess the reproducibility of heating within a repeated set of measurements on an aliquot (e.g. during the single aliquot regenerative dose protocol) providing feedback on whether changes in sample preparation can improve reproducibility. Poor reproducibility between measurements from a single aliquot are likely to arise from grains between the hotplate and sample holder. Variability between aliquots may also be affected by deformed sample holders. Differences between readers may also be caused by differences in the calibration of the hotplate. Reproducibility of heating can be improved within runs, and between instruments, by taking care to avoid loose grains, by removing deformed sample holders, and if necessary by calibrating hotplates. It is suggested that individual aliquots should only be accepted if the apparent 110 °C TL peak temperature within a sequence of measurements varies by 5 °C or less. Improving the reproducibility of thermal treatment is important in reducing scatter in equivalent dose determinations based on OSL from quartz, but also for signals such as TT-OSL from quartz, and the various feldspar luminescence signals. Temperature control is also critical for accurate kinetic analyses.

This paper provides a brief overview of the most common dating techniques applied in palaeoclimate and palaeoenvironmental studies including four radiometric and isotopic dating methods (radiocarbon, 230Th disequilibrium, luminescence, cosmogenic nuclides) and two incremental methods based on layer counting (ice layer, varves). For each method, concise background information about the fundamental principles and methodological approaches is provided. We concentrate on the time interval of focus for the INTIMATE (Integrating Ice core, MArine and TErrestrial records) community (60–8 ka). This dating guide addresses palaeoclimatologists who aim at interpretation of their often regional and local proxy time series in a wider spatial context and, therefore, have to rely on correlation with proxy records obtained from different archives from various regions. For this reason, we especially emphasise scientific approaches for harmonising chronologies for sophisticated and robust proxy data integration. In this respect, up-to-date age modelling techniques are presented as well as tools for linking records by age equivalence including tephrochronology, cosmogenic 10Be and palaeomagnetic variations. Finally, to avoid inadequate documentation of chronologies and assure reliable correlation of proxy time series, this paper provides recommendations for minimum standards of uncertainty and age datum reporting.

In this study, geomorphological, sedimentological, and geochronological work was conducted on unconfined and confined reaches of three rivers in the eastern interior of South Africa in order to quantify the relative rates of floodplain reworking and alluvial preservation along river courses with variable valley confinement and lithology. Using optically stimulated luminescence (OSL) dating techniques, new chronologies for the Schoonspruit and Mooi River were created and the existing Klip River chronology was expanded. The results suggest that floodplains in both unconfined and confined reaches preserve complex spatial and temporal patterns of alluviation, although differences in boundary conditions lead to variation in the processes and rates of floodplain construction and reworking. On the Klip and Mooi Rivers where local base levels are stable, channels in unconfined reaches rework floodplain sediments through slow lateral migration punctuated by local erosion during avulsion events. On the Schoonspruit where base level has lowered, the channel in the unconfined reach is incised, the floodplain is abandoned, and a large gully has formed. In the confined reaches of all three rivers, the narrow floodplains are reworked by scour and fill activity and limited lateral migration. The OSL results suggest that unconfined reaches preserve relatively continuous alluvial records that extend into the Pleistocene, while the floodplains in the confined reaches preserve relatively discontinuous alluvial records biased toward the late Holocene. The alluvial geochronologic records in these systems preserve signals of changes in local base level controlled by variation in lithology and incision rather than climate change. By defining the processes, rates, and patterns of floodplain reworking in reaches with different degrees of valley confinement and channel incision, the findings contribute to understanding how rivers build, modify, and preserve alluvial sedimentary archives.

The Kulshan caldera formed at ∼1.15 Ma on the present-day site of Mt. Baker, Washington State, northwest USA and erupted a compositionally zoned (dacite-rhyolite) magma and a correlative eruptive, the Lake Tapps tephra. This tephra has previously been described, but only from the Puget Lowland of NW Washington. Here an occurrence of a Kulshan caldera correlative tephra is described from the Quaternary Palouse loess at the Washtucna site (WA-3). Site WA-3 is located in east-central Washington, ∼340 km southeast of the Kulshan caldera and ∼300 km east-southeast of the Lake Tapps occurrence in the Puget Lowland. Major- and trace element chemistry and location of the deposit at Washtucna within reversed polarity sediments indicates that it is not correlative with the Mesa Falls, Rockland, Bishop Ash, Lava Creek B or Huckleberry Ridge tephras. Instead the Washtucna deposit is related to the Lake Tapps tephra by fractional crystallisation, but is chemically distinct, a consequence of its eruption from a compositionally zoned magma chamber. The correlation of the Washtucna occurrence to the Kulshan caldera-forming eruption indicates that it had an eruptive volume exceeding 100 km3, and that its tephra could provide a valuable early-Pleistocene chronostratigraphic marker in the Pacific Northwest.

Background Aggressive periodontitis is associated with the presence of Aggregatibacter actinomycetemcomitans, a leukotoxin (Ltx)-producing periodontal pathogen. Ltx has the ability to lyse white blood cells including neutrophils. Objectives This study was aimed at investigating the interactions between neutrophils and Ltx with regard to the chemotactic properties of Ltx and the release of neutrophil extracellular traps (NETs). Methods Neutrophils from healthy blood donors were isolated and incubated for 30 min and 3 h with increasing concentrations of Ltx (1, 10, and 100 ng/mL) as well as with A. actinomycetemcomitans strains (NCTC 9710 and HK 1651) producing different levels of Ltx. Formation of NETs and cell lysis were assessed by microscopy, fluorescence-based assays, and measurement of released lactate dehydrogenase. Neutrophil migration in response to different Ltx gradients was monitored by real-time video microscopy, and image analysis was performed using ImageJ software. Results Although Ltx (10 and 100 ng/mL) and the leukotoxic A. actinomycetemcomitans strain HK 1651 lysed some neutrophils, other cells were still capable of performing NETosis in a concentration-dependent manner. Low doses of Ltx and the weakly leukotoxic strain NCTC 9710 did not lead to neutrophil lysis, but did induce some NETosis. Furthermore, all three concentrations of Ltx enhanced random neutrophil movement; however, low directional accuracy was observed compared with the positive control (fMLP). Conclusions The results indicate that Ltx acts both as a neutrophil activator and also causes cell death. In addition, Ltx directly induces NETosis in neutrophils prior to cell lysis. In future studies, the underlying pathways involved in Ltx-meditated neutrophil activation and NETosis need to be investigated further.

It has previously been observed that laboratory-generated quartz optically stimulated luminescence (OSL) signals from different samples have similar dose response curves (DRCs) after they are normalized using a test dose. It therefore seems likely that growth of the normalized signal due to natural irradiation of quartz may also follow a general dose response curve. The existence of such a curve is investigated by constructing a natural DRC from the test dose-normalized natural OSL signals of seven samples from the Luochuan section of the Chinese Loess Plateau. The same aliquots are then used to build single aliquot regenerative (SAR) DRCs, making it possible to compare the natural and laboratory constructed curves. Two main differences are observed. Firstly, the laboratory-generated DRCs are best fitted with double saturating exponential functions whereas the natural DRC is equally well fitted with a single saturating function. Secondly, in the laboratory-generated DRCs the normalized OSL signal continues to increase at high laboratory doses (>500 Gy), whereas no growth is seen at these doses in the equivalent natural DRC. These differences between natural- and laboratory-generated DRCs are still apparent even if data are manipulated to isolate the fast component, or if a sensitivity corrected multiple aliquot regenerative (SC-MAR) dose procedure is used. This suggests that the observed differences are not due to the influence of different components or inter-regenerative dose cycle sensitivity changes. The divergence between the natural- and laboratory-generated DRC means that the current maximum limit of quartz OSL dating at the Luochuan section is 150 Gy, as D e estimates above this value are likely to be underestimations.

Concern over anomalous fading has been the biggest single factor responsible for deterring the widespread use of the infra-red stimulated luminescence (IRSL) or thermoluminescence (TL) signal from feldspars for luminescence dating. There has therefore been great interest in the use of the recently proposed Post-IR IRSL signal, because it has been shown to significantly reduce the degree of anomalous fading observed in feldspars and therefore potentially provides a means of circumventing the issue. This study undertakes a systematic investigation into various preheat and Post-IR IRSL measurement conditions proposed in the literature, by using two samples from the Halfway House loess section in Alaska which bracket the Old Crow tephra which has been dated using fission track methods. Preheat plateau tests show a dramatic change in equivalent dose with Post-IR IRSL measurement conditions, and further tests reveal that these changes are driven by preheat temperature rather than Post-IR IR stimulation temperature. Dose recovery tests on laboratory-bleached material mimic the findings of the natural preheat plateau test data, and sensitivity change between the first and second Single Aliquot Regenerative dose (SAR) measurement cycle is found to be responsible. Comparison of the Post-IR IRSL ages with the independent age control shows that, for the samples in this study, the Post-IR IR signal stimulated at 290 °C is inappropriate for dating. However, use of lower preheat (250–300 °C) and Post-IR IR stimulation temperatures from 225 to 270 °C gave rise to ages which were in agreement with the independent age control.