Search

Your search keyword '"Hemkemeyer, Sandra A"' showing total 19 results
Start Over Author "Hemkemeyer, Sandra A"
19 results on '"Hemkemeyer, Sandra A"'

1. Lymphatic Mechanoregulation in Development and Disease

Author

Hemkemeyer, Sandra A., Carlantoni, Claudia, Frye, Maike, Karamanos, Nikos K., Series Editor, Kletsas, Dimitris, Editorial Board Member, Oh, Eok-Soo, Editorial Board Member, Passi, Alberto, Editorial Board Member, Pihlajaniemi, Taina, Editorial Board Member, Ricard-Blum, Sylvie, Editorial Board Member, Sagi, Irit, Editorial Board Member, Savani, Rashmin, Editorial Board Member, Watanabe, Hideto, Editorial Board Member, Papadimitriou, Evangelia, editor, and Mikelis, Constantinos M., editor

Published
2023
Full Text
View/download PDF

6. The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling

Author

Carlantoni, Claudia, primary, Liekfeld, Leon, additional, Hemkemeyer, Sandra A., additional, Schreier, Danny, additional, Saygi, Ceren, additional, Kurelic, Roberta, additional, Cardarelli, Silvia, additional, Kalucka, Joanna, additional, Schulte, Christian, additional, Beerens, Manu, additional, Mailer, Reiner, additional, Schäffer, Tilman, additional, Naro, Fabio, additional, Pellegrini, Manuela, additional, Nikolaev, Viacheslav O., additional, Renné, Thomas, additional, and Frye, Maike, additional

Published
2023
Full Text
View/download PDF

8. Therapeutic Validation of GEF-H1 Using a De Novo Designed Inhibitor in Models of Retinal Disease

Author

Mills, Clare, primary, Hemkemeyer, Sandra A., additional, Alimajstorovic, Zerin, additional, Bowers, Chantelle, additional, Eskandarpour, Malihe, additional, Greenwood, John, additional, Calder, Virginia, additional, Chan, A. W. Edith, additional, Gane, Paul J., additional, Selwood, David L., additional, Matter, Karl, additional, and Balda, Maria S., additional

Published
2022
Full Text
View/download PDF

9. A Thromboxane A 2 Receptor-Driven COX-2–Dependent Feedback Loop That Affects Endothelial Homeostasis and Angiogenesis

Author

Eckenstaler, Robert, primary, Ripperger, Anne, additional, Hauke, Michael, additional, Petermann, Markus, additional, Hemkemeyer, Sandra A., additional, Schwedhelm, Edzard, additional, Ergün, Süleyman, additional, Frye, Maike, additional, Werz, Oliver, additional, Koeberle, Andreas, additional, Braun, Heike, additional, and Benndorf, Ralf A., additional

Published
2022
Full Text
View/download PDF

13. Local Myo9b RhoGAP activity regulates cell motility

Author

Hemkemeyer, Sandra A., primary, Vollmer, Veith, additional, Schwarz, Vera, additional, Lohmann, Birgit, additional, Honnert, Ulrike, additional, Taha, Muna, additional, Schnittler, Hans-Joachim, additional, and Bähler, Martin, additional

Published
2021
Full Text
View/download PDF

15. Multiple roles of filopodial dynamics in particle capture and phagocytosis and phenotypes of Cdc42 and Myo10 deletion

Author

Horsthemke, Markus, primary, Bachg, Anne C., additional, Groll, Katharina, additional, Moyzio, Sven, additional, Müther, Barbara, additional, Hemkemeyer, Sandra A., additional, Wedlich-Söldner, Roland, additional, Sixt, Michael, additional, Tacke, Sebastian, additional, Bähler, Martin, additional, and Hanley, Peter J., additional

Published
2017
Full Text
View/download PDF

16. Dendritic Cell Motility and T Cell Activation Requires Regulation of Rho-Cofilin Signaling by the Rho-GTPase Activating Protein Myosin IXb

Author

Xu, Yan, primary, Pektor, Stefanie, additional, Balkow, Sandra, additional, Hemkemeyer, Sandra A., additional, Liu, Zhijun, additional, Grobe, Kay, additional, Hanley, Peter J., additional, Shen, Limei, additional, Bros, Matthias, additional, Schmidt, Talkea, additional, Bähler, Martin, additional, and Grabbe, Stephan, additional

Published
2014
Full Text
View/download PDF

17. MarvelD3 couples tight junctions to the MEKK1–JNK pathway to regulate cell behavior and survival

Author

Steed, Emily, primary, Elbediwy, Ahmed, additional, Vacca, Barbara, additional, Dupasquier, Sébastien, additional, Hemkemeyer, Sandra A., additional, Suddason, Tesha, additional, Costa, Ana C., additional, Beaudry, Jean-Bernard, additional, Zihni, Ceniz, additional, Gallagher, Ewen, additional, Pierreux, Christophe E., additional, Balda, Maria S., additional, and Matter, Karl, additional

Published
2014
Full Text
View/download PDF

18. Abstract 13973: Human iPSC-Derived Multi-Cell-Type Engineered Heart Tissues as an in vitroModel to Study Pathological Cardiac Hypertrophy

Author

Wrona, Kinga M, Albert, Alba M. M, Eckardt, Dominik, Herbel, Christoph, Krause, Julia, Starbatty, Jutta, Hemkemeyer, Sandra A A, Frye, Maike, Cuello, Friederike, Eschenhagen, Thomas, and Hirt, Marc N

Abstract

IntroductionPathological cardiac hypertrophy has not been fully recapitulated in the in vitrosettings. Currently used in vitromodels are presumably oversimplified since the vast majority are based on iPSC-derived cardiomyocytes (CMs) exclusively and do not include any cardiac non-myocytes. The aim of this study was to generate engineered heart tissues (EHTs) containing five hiPSC-derived cardiac cell types, i.e., CMs, endothelial cells (ECs), macrophages (Mϕs), fibroblasts (FBs), and smooth muscle cells (SMCs) and treat them with prohypertrophic compounds.Methods:Mesoderm induction preceded all cell-type-specific differentiation steps. CMs were then generated by Wnt signaling inhibition and ECs by VEGF stimulation. Mϕ differentiation required treatment with IL-3+M-CSF. FBs and SMCs were generated by Wnt pathway activation followed by bFGF or TGFβ+bFGF stimulation. The characterization of different iPSC-derivatives and EHTs was based on the MICS (MACSima Imaging Cycling Staining) technology and profiling of hallmark genes and proteins. To study the response to prohypertrophic stimuli, EHTs were treated with 20 μM phenylephrine (PE) and 50 nM endothelin-1 (ET-1) for 9-10 days.Results:The expression of cell-type-specific markers and functional assays were the determinants of successful differentiation. For instance, CMs were identified based on cTnT expression and contractile capabilities, Mϕs by expression of CD68 and phagocytic activity, and ECs by CD31 expression and tube formation abilities. Multi-cell-type EHTs were assembled from 60% CMs, 20% ECs, 10% Mϕs, 5% FBs and 5% SMCs. These EHTs displayed accelerated development, i.e., faster remodeling and earlier onset of beating compared to CM-only EHTs. Preliminary results show that multi-cell-type EHTs exhibited higher maximal forces and increased stiffness compared to CM-only controls. The exposure to PE+ET-1 led to a decrease in force, activation of the hypertrophic gene program, and elevation of NT-pro BNP production.Conclusions:We successfully generated functional multi-cell-type EHTs containing five hiPSC-derived cardiac cell types. Upon hypertrophic stimulation, these EHTs developed a pathological phenotype that resembles the hallmarks of a failing human heart.

Published
2022
Full Text
View/download PDF

19. Dendritic Cell Motility and T Cell Activation Requires Regulation of Rho-Cofilin Signaling by the Rho-GTPase Activating Protein Myosin IXb.

Author

Yan Xu, Pektor, Stefanie, Balkow, Sandra, Hemkemeyer, Sandra A., Zhijun Liu, Grobe, Kay, Hanley, Peter J., Limei Shen, Bros, Matthias, Schmidt, Talkea, Bähler, Martin, and Grabbe, Stephan

Subjects
*DENDRITIC cells, *CELL migration, *LYMPHOID tissue, *T cells, *IMMUNE response, *MYOSIN
Abstract

Directed migration of stimulated dendritic cells (DCs) to secondary lymphoid organs and their interaction with Ag-specific T cells is a prerequisite for the induction of primary immune responses. In this article, we show that murine DCs that lack myosin IXB (Myo9b), a motorized negative regulator of RhoA signaling, exhibit increased Rho signaling activity and downstream actomyosin contractility, and inactivation of the Rho target protein cofilin, an actin-depolymerizing factor. On a functional level, Myo9b-/- DCs showed impaired directed migratory activity both in vitro and in vivo. Moreover, despite unaltered Ag presentation and costimulatory capabilities, Myo9b-/- DCs were poor T cell stimulators in vitro in a three-dimensional collagen matrix and in vivo, associated with altered DC-T cell contact dynamics and T cell polarization. Accordingly, Myo9b-/- mice showed an attenuated ear-swelling response in a model of contact hypersensitivity. The impaired migratory and T cell stimulatory capacity of Myo9b-/- DCs was restored in large part by pharmacological activation of cofilin. Taken together, these results identify Myo9b as a negative key regulator of the Rho/RhoA effector Rho-kinase [Rho-associated coiled-coil-forming kinase (ROCK)]/LIM domain kinase signaling pathway in DCs, which controls cofilin inactivation and myosin II activation and, therefore may control, in part, the induction of adaptive immune responses. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results