1. Four amino acids play an important role in the allergenicity of hemocyanin allergen.
- Author
-
Luan H, Lu J, Shi W, and Lu Y
- Subjects
- Animals, Humans, Mice, Caco-2 Cells, Immunoglobulin E immunology, Epitopes immunology, Brachyura immunology, Cytokines metabolism, Amino Acid Sequence, Mutation, Allergens immunology, Allergens genetics, Allergens chemistry, Hemocyanins immunology, Hemocyanins chemistry, Amino Acids immunology, Mast Cells immunology, Mast Cells metabolism
- Abstract
To identify the key amino acids (AAs) affecting the allergenicity of hemocyanin (HC) allergens from Chinese mitten crabs, in this study, two epitopes, P1-SHFTGSKSNPEQR and P2-LSPGANTITR were employed and four potential key AAs (P1: F3 and N9 and P2: N6 and R10) were predicted. Mast cell and mouse models revealed that four mutants induced lower levels of immunoglobulin E (IgE) and Th2 type cytokines (15.47-49.89 %), proving that F3, N9, N6, and R10 were the key AAs of two epitopes. Mutants reduce allergic responses via the Th2 pathway. However, the roles of every key AA affecting allergenicity were different (P1-F3 > N9 and P2-N6 > R10). In addition, lower transport and higher efflux were observed in the mutants during transport absorption by Caco-2 cells. The allergenicity of HC was stronger when the transport absorption efficiency of epitopes and mutants was higher and their efflux was lower. Our study provides a novel method for revealing the allergenic molecular mechanisms of food allergens., Competing Interests: Declaration of competing interest The authors attested that there were no conflicts of interest concerning this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF