Search

Your search keyword '"Herbert, Jacquie F."' showing total 17 results
Start Over Author "Herbert, Jacquie F."
17 results on '"Herbert, Jacquie F."'

13. Placental function in lizards

Author

Thompson, Michael B., primary, Adams, Susan M., additional, Herbert, Jacquie F., additional, Biazik, Joanna M., additional, and Murphy, Christopher R., additional

Published
2004
Full Text
View/download PDF

16. Uptake of dextran‐FITC by epithelial cells of the chorioallantoic placentome and the omphalopleure of the placentotrophic lizard, Pseudemoia entrecasteauxii

Author

Stewart, James R., Thompson, Michael B., Attaway, Marcus B., Herbert, Jacquie F., and Murphy, Christopher R.

Abstract

Placental nutrient provision has evolved in multiple lineages of squamate reptiles and although possible structural specializations for placentotrophy have been described in a variety of species, neither the pathways nor the mechanisms of placental transfer are known. Lizards of the Australian genus Pseudemoia are placentotrophic and have elaborate placental structures that are thought to enhance nutrient transfer. The chorioallantoic placenta, which occupies the embryonic hemisphere of the egg, is regionally diversified into a large area with low epithelial height and a smaller placentome with cuboidal or columnar epithelia. Both regions are underlain by an extensive vascular bed. The abembryonic hemisphere of the egg is covered by an omphaloplacenta, which is similar to the placentome in having cuboidal or columnar epithelia but with a different embryonic vascular supply. We tested the hypothesis that embryonic epithelial cells of the placentome and the omphaloplacenta of Pseudemoia entrecasteauxii are each capable of endocytosis. Embryos (stages 33–39) with intact extraembryonic membranes were surgically removed from the uterus and incubated in a solution containing fluorescein isothiocyanate‐dextran (77,000 MW). The fluorescent label was detected in the cytoplasm of scattered populations of epithelial cells in both placental regions of all embryonic stages. We conclude that both the placentome and the omphaloplacenta of P. entrecasteauxii are sites of histotrophic nutrient transport. However, there are histological and cytological differences in the embryonic epithelia of these two placental regions. The histological differences reflect differences in the evolutionary precursors of each tissue. The cytological differences likely portray different functional specializations. J. Exp. Zool. 305, 2006. © 2006 Wiley‐Liss, Inc.

Published
2006
Full Text
View/download PDF

17. Expression and localization of Ca2+-ATPase in the uterus during the reproductive cycle of king quail (Coturnix chinensis) and zebra finch (Poephila guttata)

Author

Parker, Scott L., Lindsay, Laura A., Herbert, Jacquie F., Murphy, Christopher R., and Thompson, Michael B.

Subjects
*ADENOSINE triphosphatase, *QUAILS, *CALCIUM, *ENZYMES
Abstract

Abstract: Calcium ATPase (Ca2+-ATPase) is a key enzyme that participates in the translocation of calcium in the uterus of oviparous amniotes during eggshell formation. We used Western blot and indirect immunofluorescence microscopy to determine expression and localisation of uterine Ca2+-ATPase during the reproductive cycle of king quail and zebra finch. The pattern of Ca2+-ATPase expression and localisation during the reproductive cycle was similar for both species. Immunoblots of uterine extracts from quail and finch indicated that Ca 2+-ATPase expression is reduced in non-reproductive compared to reproductive females. Similarly, in non-reproductive females, weak apical immunofluorescent staining of Ca2+-ATPase is localised to epithelial cells in a small number of uterine tubular glands. A large increase in apical immunofluorescent staining of tubular gland epithelia occurs in both vitellogenic and reproductive females. The presence of Ca2+-ATPase on the apical surface of tubular gland epithelial cells suggests that the enzyme is involved in the translocation of calcium out of the tubular gland epithelia and into the concentrated fluid of the uterine lumen. Presence of Ca2+-ATPase in vitellogenic females indicates that the enzyme is expressed prior to the time of ovulation and eggshell calcification. [Copyright &y& Elsevier]

Published
2008
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results