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Your search keyword '"Hermsen, Harm P.H."' showing total 16 results
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16 results on '"Hermsen, Harm P.H."'

1. Mimicking of K(sup)+ activation by double mutation of glutamate 795 and glutamate 820 of gastric H(sup)+, K(sup)+ -ATPase

Author

Hermsen, Harm P.H., Swarts, Herman G.P., Wassink, Lianne, Koenderink, Jan B., Willems, Peter H.G.M., and De Pont, Jan Joep H.H.M.

Subjects
Enzymes -- Structure-activity relationship, Amino acids -- Structure-activity relationships, Mutation (Biology) -- Analysis, Adenosine triphosphatase -- Physiological aspects, Biological sciences, Chemistry
Abstract

Results show that mutants Glu820 and Glu795 of the rat gastric proton, potassium- ATPase exhibit ATPase activity constitutively and increased ATP phosphorylation as shown by double mutations, involving replacement of glutamate residues with glutamine and alanine at positions 795 and 820, respectively.

Published
2001

2. The carbonyl group of glutamic acid-795 is essential for gastric H (super)+,K (super)+ -ATPase activity

Author

Hermsen, Harm P.H., Koenderink, Jan B., Swarts, Herman G.P., and De Pont, Jan Joep H.H.M.

Subjects
Biochemistry -- Research, Glutamate -- Research, Gastritis -- Prevention, Cells -- Analysis, Mutation (Biology) -- Analysis, Biological sciences, Chemistry
Abstract

Research has been conducted on the glutamic acid Glu (super)795. The generation and expression of mutants in Sf9 insect cells are described.

Published
2000

11. High-affinity ouabain binding by a chimeric gastric H+, K+-ATPase containing transmembrane...

Author

Koenderink, Jan B and Hermsen, Harm P.H.

Subjects
*PROTEIN binding, *BIOTRANSFORMATION (Metabolism)
Abstract

Investigates the role of different transmembrane helices or hairpins in the binding of the drug ouabain. Transmembrane region involved in the specific interaction of the ouabain with enzymes responsible for active cation transport; Dephosphorylation of the phosphorylated enzymes.

Published
2000
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15. Teh negatively charged glutamic acid 820 of the gastric H+,K+-ATPase α-subunit plays an essential role in K+ binding and transition of the E2 form.

Author

Hermsen, Harm P.H., Swarts, Herman G.P., Koenderink, Jan B., and H.H.M. De Pont, Jan Joep

Subjects
*HYDROGEN/POTASSIUM ATPase, *GLUTAMIC acid
Abstract

Examines the essential role of negatively charged glutamic acid of the gastric hydrogen/potassium ATPase in potassium binding. Phosphorylation of mutant cells; Ability of the mutant to form a phosphorylated intermediate; Inhibition of enzymes.

Published
1998

16. Functional expression of Na+,K+-ATPase and their hybrids in insect cells.

Author

Koenderink, Jan B., Swarts, Herman G.P., Hermsen, Harm P.H., and H.H.M. De Pont, Jan Joep

Subjects
*ADENOSINE triphosphatase, *INSECT cell biotechnology
Abstract

Examines the functional expression of sodium/potassium ATPase and hydrogen/potassium ATPase in insect cells. Structural features of the adenosine triphosphatase (ATP); Information about regions responsible for drug and cation specificity; Measurement of the ATP-phosphorylation levels.

Published
1998

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