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8. Novel lipid emulsion for total parenteral nutrition based on 18-carbon n–3 fatty acids elicits a superior immunometabolic phenotype in a murine model compared with standard lipid emulsions

Author

Lucchinetti, Eliana, Lou, Phing-How, Holtzhauer, Gregory, Noureddine, Nazek, Wawrzyniak, Paulina, Hartling, Ivan, Lee, Megan, Strachan, Erin, Clemente-Casares, Xavier, Tsai, Sue, Rogler, Gerhard, Krämer, Stefanie D, Hersberger, Martin, and Zaugg, Michael

Published
2022
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14. The Novel Lipid Emulsion Vegaven Is Well Tolerated and Elicits Distinct Biological Actions Compared With a Mixed-Oil Lipid Emulsion Containing Fish Oil: A Parenteral Nutrition Trial in Piglets

Author

Lucchinetti, Eliana; https://orcid.org/0000-0003-3338-1279, Lou, Phing-How; https://orcid.org/0000-0003-2090-1132, Chakravarty, Akash; https://orcid.org/0000-0002-3022-1539, Marcolla, Camila Schultz; https://orcid.org/0000-0002-0010-4841, Pauline, Mirielle L; https://orcid.org/0000-0001-8700-0035, Wizzard, Pamela R; https://orcid.org/0000-0002-3567-1846, Field, Catherine J; https://orcid.org/0000-0001-7285-4767, Wine, Eytan; https://orcid.org/0000-0002-3458-4142, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Wales, Paul W; https://orcid.org/0000-0001-7206-735X, Turner, Justine M; https://orcid.org/0000-0002-5540-6660, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, Zaugg, Michael; https://orcid.org/0000-0002-2016-8973, Lucchinetti, Eliana; https://orcid.org/0000-0003-3338-1279, Lou, Phing-How; https://orcid.org/0000-0003-2090-1132, Chakravarty, Akash; https://orcid.org/0000-0002-3022-1539, Marcolla, Camila Schultz; https://orcid.org/0000-0002-0010-4841, Pauline, Mirielle L; https://orcid.org/0000-0001-8700-0035, Wizzard, Pamela R; https://orcid.org/0000-0002-3567-1846, Field, Catherine J; https://orcid.org/0000-0001-7285-4767, Wine, Eytan; https://orcid.org/0000-0002-3458-4142, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Wales, Paul W; https://orcid.org/0000-0001-7206-735X, Turner, Justine M; https://orcid.org/0000-0002-5540-6660, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, and Zaugg, Michael; https://orcid.org/0000-0002-2016-8973

Published
2024

15. Size of lipid emulsion droplets influences metabolism in human CD4+ T cells

Author

Noureddine, Nazek; https://orcid.org/0000-0003-0308-5807, Holtzhauer, Gregory; https://orcid.org/0000-0002-5560-2354, Wawrzyniak, Paulina; https://orcid.org/0000-0001-9641-2103, Srikanthan, Pakeerathan, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, Rogler, Gerhard; https://orcid.org/0000-0002-1733-9188, Lucchinetti, Eliana; https://orcid.org/0000-0003-3338-1279, Zaugg, Michael; https://orcid.org/0000-0002-2016-8973, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Noureddine, Nazek; https://orcid.org/0000-0003-0308-5807, Holtzhauer, Gregory; https://orcid.org/0000-0002-5560-2354, Wawrzyniak, Paulina; https://orcid.org/0000-0001-9641-2103, Srikanthan, Pakeerathan, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, Rogler, Gerhard; https://orcid.org/0000-0002-1733-9188, Lucchinetti, Eliana; https://orcid.org/0000-0003-3338-1279, Zaugg, Michael; https://orcid.org/0000-0002-2016-8973, and Hersberger, Martin; https://orcid.org/0000-0002-4472-329X

Published
2024

16. Investigating thyroid function and iodine status in adolescents with and without paediatric major depressive disorder

Author

Osuna, Ester; https://orcid.org/0000-0001-7706-6102, Baumgartner, Jeannine; https://orcid.org/0000-0002-9107-4181, Walther, Andreas; https://orcid.org/0000-0003-4516-1783, Emery, Sophie; https://orcid.org/0000-0002-4073-6771, Albermann, Mona, Baumgartner, Noemi; https://orcid.org/0000-0001-8368-6316, Schmeck, Klaus; https://orcid.org/0000-0003-3114-902X, Walitza, Susanne; https://orcid.org/0000-0002-8161-8683, Strumberger, Michael A; https://orcid.org/0000-0001-7929-4328, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Zimmermann, Michael B, Häberling, Isabelle, Berger, Gregor E; https://orcid.org/0000-0003-2030-141X, Herter-Aeberli, Isabelle; https://orcid.org/0000-0003-0134-6217, Omega-3 study team, Osuna, Ester; https://orcid.org/0000-0001-7706-6102, Baumgartner, Jeannine; https://orcid.org/0000-0002-9107-4181, Walther, Andreas; https://orcid.org/0000-0003-4516-1783, Emery, Sophie; https://orcid.org/0000-0002-4073-6771, Albermann, Mona, Baumgartner, Noemi; https://orcid.org/0000-0001-8368-6316, Schmeck, Klaus; https://orcid.org/0000-0003-3114-902X, Walitza, Susanne; https://orcid.org/0000-0002-8161-8683, Strumberger, Michael A; https://orcid.org/0000-0001-7929-4328, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Zimmermann, Michael B, Häberling, Isabelle, Berger, Gregor E; https://orcid.org/0000-0003-2030-141X, Herter-Aeberli, Isabelle; https://orcid.org/0000-0003-0134-6217, and Omega-3 study team

Published
2024

17. Iron status in Swiss adolescents with paediatric major depressive disorder and healthy controls: a matched case–control study

Author

Osuna, Ester; https://orcid.org/0000-0001-7706-6102, Baumgartner, Jeannine; https://orcid.org/0000-0002-9107-4181, Wunderlin, Olivia, Emery, Sophie; https://orcid.org/0000-0002-4073-6771, Albermann, Mona, Baumgartner, Noemi; https://orcid.org/0000-0001-8368-6316, Schmeck, Klaus; https://orcid.org/0000-0003-3114-902X, Walitza, Susanne; https://orcid.org/0000-0002-8161-8683, Strumberger, Michael, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Zimmermann, Michael B, Häberling, Isabelle, Berger, Gregor E; https://orcid.org/0000-0003-2030-141X, Herter-Aeberli, Isabelle; https://orcid.org/0000-0003-0134-6217, Omega-3 Study Team, Drechsler, Renate; https://orcid.org/0000-0002-4243-6596, Grünblatt, Edna; https://orcid.org/0000-0001-8505-7265, et al, Osuna, Ester; https://orcid.org/0000-0001-7706-6102, Baumgartner, Jeannine; https://orcid.org/0000-0002-9107-4181, Wunderlin, Olivia, Emery, Sophie; https://orcid.org/0000-0002-4073-6771, Albermann, Mona, Baumgartner, Noemi; https://orcid.org/0000-0001-8368-6316, Schmeck, Klaus; https://orcid.org/0000-0003-3114-902X, Walitza, Susanne; https://orcid.org/0000-0002-8161-8683, Strumberger, Michael, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Zimmermann, Michael B, Häberling, Isabelle, Berger, Gregor E; https://orcid.org/0000-0003-2030-141X, Herter-Aeberli, Isabelle; https://orcid.org/0000-0003-0134-6217, Omega-3 Study Team, Drechsler, Renate; https://orcid.org/0000-0002-4243-6596, Grünblatt, Edna; https://orcid.org/0000-0001-8505-7265, and et al

Published
2024

18. Expert consensus report on lipid mediators: Role in resolution of inflammation and muscle preservation

Author

Serhan, Charles N; https://orcid.org/0000-0003-4627-8545, Bäck, Magnus; https://orcid.org/0000-0003-0853-5141, Chiurchiù, Valerio; https://orcid.org/0000-0002-0316-7311, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Mittendorfer, Bettina; https://orcid.org/0000-0002-1746-8545, Calder, Philip C; https://orcid.org/0000-0002-6038-710X, Waitzberg, Dan L; https://orcid.org/0000-0002-9196-9372, Stoppe, Christian; https://orcid.org/0000-0002-2028-2039, Klek, Stanislaw; https://orcid.org/0000-0002-7887-3464, Martindale, Robert G; https://orcid.org/0000-0002-9159-4486, Serhan, Charles N; https://orcid.org/0000-0003-4627-8545, Bäck, Magnus; https://orcid.org/0000-0003-0853-5141, Chiurchiù, Valerio; https://orcid.org/0000-0002-0316-7311, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Mittendorfer, Bettina; https://orcid.org/0000-0002-1746-8545, Calder, Philip C; https://orcid.org/0000-0002-6038-710X, Waitzberg, Dan L; https://orcid.org/0000-0002-9196-9372, Stoppe, Christian; https://orcid.org/0000-0002-2028-2039, Klek, Stanislaw; https://orcid.org/0000-0002-7887-3464, and Martindale, Robert G; https://orcid.org/0000-0002-9159-4486

Abstract

This meeting report presents a consensus on the biological aspects of lipid emulsions in parenteral nutrition, emphasizing the unanimous support for the integration of lipid emulsions, particularly those containing fish oil, owing to their many potential benefits beyond caloric provision. Lipid emulsions have evolved from simple energy sources to complex formulations designed to improve safety profiles and offer therapeutic benefits. The consensus highlights the critical role of omega‐3 polyunsaturated fatty acids (PUFAs), notably eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), found in fish oil and other marine oils, for their anti‐inflammatory properties, muscle mass preservation, and as precursors to the specialized pro‐resolving mediators (SPMs). SPMs play a significant role in immune modulation, tissue repair, and the active resolution of inflammation without impairing host defense mechanisms. The panel's agreement underscores the importance of incorporating fish oil within clinical practices to facilitate recovery in conditions like surgery, critical illness, or immobility, while cautioning against therapies that might disrupt natural inflammation resolution processes. This consensus not only reaffirms the role of specific lipid components in enhancing patient outcomes, but also suggests a shift towards nutrition‐based therapeutic strategies in clinical settings, advocating for the proactive evidence‐based use of lipid emulsions enriched with omega‐3 PUFAs. Furthermore, we should seek to apply our knowledge concerning DHA, EPA, and their SPM derivatives, to produce more informative randomized controlled trial protocols, thus allowing more authoritative clinical recommendations.

Published
2024

19. The EBI2-oxysterol axis promotes the development of intestinal lymphoid structures and colitis

Author

Wyss, Annika, Raselli, Tina, Perkins, Nathan, Ruiz, Florian, Schmelczer, Gérard, Klinke, Glynis, Moncsek, Anja, Roth, René, Spalinger, Marianne R., Hering, Larissa, Atrott, Kirstin, Lang, Silvia, Frey-Wagner, Isabelle, Mertens, Joachim C., Scharl, Michael, Sailer, Andreas W., Pabst, Oliver, Hersberger, Martin, Pot, Caroline, Rogler, Gerhard, and Misselwitz, Benjamin

Published
2019
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25. Expert consensus report on lipid mediators: Role in resolution of inflammation and muscle preservation.

Author

Serhan, Charles N., Bäck, Magnus, Chiurchiù, Valerio, Hersberger, Martin, Mittendorfer, Bettina, Calder, Philip C., Waitzberg, Dan L., Stoppe, Christian, Klek, Stanislaw, Martindale, Robert G., Calder, Philip, Cogle, Sarah, Evans, David, Gramlich, Leah, Martindale, Robert, McClave, Stephen, Mundi, Manpreet, Muscaritoli, Maurizio, Nishikawa, Reid, and Patel, Jayshil

Published
2024
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29. Associations of n-3 polyunsaturated fatty acid status and intake with paediatric major depressive disorder in Swiss adolescents: A case-control study

Author

Osuna, Ester; https://orcid.org/0000-0001-7706-6102, Herter-Aeberli, Isabelle; https://orcid.org/0000-0003-0134-6217, Probst, Silvia, Emery, Sophie; https://orcid.org/0000-0002-4073-6771, Albermann, Mona, Baumgartner, Noemi; https://orcid.org/0000-0001-8368-6316, Strumberger, Michael, Ricci, Cristian, Schmeck, Klaus, Walitza, Susanne; https://orcid.org/0000-0002-8161-8683, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Zimmermann, Michael B, Häberling, Isabelle, Berger, Gregor; https://orcid.org/0000-0003-2030-141X, Baumgartner, Jeannine, Osuna, Ester; https://orcid.org/0000-0001-7706-6102, Herter-Aeberli, Isabelle; https://orcid.org/0000-0003-0134-6217, Probst, Silvia, Emery, Sophie; https://orcid.org/0000-0002-4073-6771, Albermann, Mona, Baumgartner, Noemi; https://orcid.org/0000-0001-8368-6316, Strumberger, Michael, Ricci, Cristian, Schmeck, Klaus, Walitza, Susanne; https://orcid.org/0000-0002-8161-8683, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Zimmermann, Michael B, Häberling, Isabelle, Berger, Gregor; https://orcid.org/0000-0003-2030-141X, and Baumgartner, Jeannine

Abstract

Background: Observational studies suggest a link between n-3 polyunsaturated fatty acid (PUFA) intake, n-3 PUFA status, and depression in adults, but studies in adolescents are scarce. This study aimed to determine associations of n-3 PUFA status and intake with paediatric major depressive disorder (pMDD) in Swiss adolescents. Methods: We conducted a matched case-control study in 95 adolescents diagnosed with pMDD and 95 healthy controls aged 13 to <18 years. We analysed red blood cell (RBC) fatty acid (FA) composition (% of total FA). n-3 PUFA intake was assessed using a focused food frequency questionnaire and depression severity was assessed by the Children's Depression Rating Scale-Revised (CDRS-R). Results: Mean RBC eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were lower in cases than controls (EPA:0.41 ± 0.11 vs 0.46 ± 0.12, p < 0.001; DHA:4.07 ± 1.04 vs 4.73 ± 1.04, p < 0.001). Subsequently, the mean RBC n-3 index was lower (4.51 ± 1.10 vs 5.20 ± 1.11, p < 0.001) and the n-6/n-3 PUFA ratio higher (5.51 ± 1.25 vs 4.96 ± 1.08, p < 0.001) in cases than controls. Adolescents with a higher n-3 index had lower odds for depression (OR = 0.49 [95%CI:0.32-0.71]). In contrast, the n-6/n-3 PUFA ratio was associated with higher odds for depression (OR = 1.58 [95%CI:1.14-2.25]). Intake of alpha-linolenic acid, EPA and DHA did not differ between cases and controls. Conclusion: Our results suggest that a higher RBC n-3 PUFA status during adolescence is associated with a lower risk for pMDD, whereas a higher n-6/n-3 PUFA ratio is associated with a higher risk for pMDD. Differences in n-3 PUFA intake did not explain the observed differences in n-3 PUFA status.

Published
2023

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Author

Cambiaggi, Lucia, Chakravarty, Akash; https://orcid.org/0000-0002-3022-1539, Noureddine, Nazek; https://orcid.org/0000-0003-0308-5807, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Cambiaggi, Lucia, Chakravarty, Akash; https://orcid.org/0000-0002-3022-1539, Noureddine, Nazek; https://orcid.org/0000-0003-0308-5807, and Hersberger, Martin; https://orcid.org/0000-0002-4472-329X

Published
2023

31. Crosstalk within peripheral blood mononuclear cells mediates anti-inflammatory effects of n-3 PUFA-rich lipid emulsions in parenteral nutrition

Author

Wawrzyniak, Paulina; https://orcid.org/0000-0001-9641-2103, Hubeli, Barbara, Wawrzyniak, Marcin; https://orcid.org/0000-0002-6911-8010, Noureddine, Nazek; https://orcid.org/0000-0003-0308-5807, Walberg, Asa; https://orcid.org/0000-0002-6922-8375, Scharl, Sylvie, Turina, Matthias; https://orcid.org/0000-0002-1040-3511, Scharl, Michael; https://orcid.org/0000-0002-6729-1469, Zaugg, Michael; https://orcid.org/0000-0002-2016-8973, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, Rogler, Gerhard; https://orcid.org/0000-0002-1733-9188, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Wawrzyniak, Paulina; https://orcid.org/0000-0001-9641-2103, Hubeli, Barbara, Wawrzyniak, Marcin; https://orcid.org/0000-0002-6911-8010, Noureddine, Nazek; https://orcid.org/0000-0003-0308-5807, Walberg, Asa; https://orcid.org/0000-0002-6922-8375, Scharl, Sylvie, Turina, Matthias; https://orcid.org/0000-0002-1040-3511, Scharl, Michael; https://orcid.org/0000-0002-6729-1469, Zaugg, Michael; https://orcid.org/0000-0002-2016-8973, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, Rogler, Gerhard; https://orcid.org/0000-0002-1733-9188, and Hersberger, Martin; https://orcid.org/0000-0002-4472-329X

Abstract

Background and aims: Parenteral nutrition (PN) rich in n-6 and n-3 long-chain fatty acids is used in clinical practice for nourishing patients who are unable to receive adequate nutrition through their digestive systems. In this study, we compare the effect on inflammation of the commonly used lipid emulsions Omegaven (n-3-rich) and Intralipid (n-6-rich) in human peripheral blood mononuclear cells (PBMCs).

Published
2023

33. Improved diagnostics of purine and pyrimidine metabolism disorders using LC-MS/MS and its clinical application

Author

Cremonesi, Alessio, Meili, David, Rassi, Anahita, Poms, Martin, Tavazzi, Barbara, Škopová, Václava, Häberle, Johannes, Zikánová, Marie, Hersberger, Martin, University of Zurich, and Cremonesi, Alessio

Subjects
10036 Medical Clinic, Biochemistry (medical), Clinical Biochemistry, 610 Medicine & health, General Medicine, 1308 Clinical Biochemistry, 2704 Biochemistry (medical)
Abstract

Objectives To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantify 41 different purine and pyrimidine (PuPy) metabolites in human urine to allow detection of most known disorders in this metabolic pathway and to determine reference intervals. Methods Urine samples were diluted with an aqueous buffer to minimize ion suppression. For detection and quantification, liquid chromatography was combined with electrospray ionization, tandem mass spectrometry and multiple reaction monitoring. Transitions and instrument settings were established to quantify 41 analytes and nine stable-isotope-labeled internal standards (IS). Results The established method is precise (intra-day CV: 1.4–6.3%; inter-day CV: 1.3–15.2%), accurate (95.2% external quality control results within ±2 SD and 99.0% within ±3 SD; analyte recoveries: 61–121%), sensitive and has a broad dynamic range to quantify normal and pathological metabolite concentrations within one run. All analytes except aminoimidazole ribonucleoside (AIr) are stable before, during and after sample preparation. Moreover, analytes are not affected by five cycles of freeze-thawing (variation: −5.6 to 7.4%), are stable in thymol (variation: −8.4 to 12.9%) and the lithogenic metabolites also in HCl conserved urine. Age-dependent reference intervals from 3,368 urine samples were determined and used to diagnose 11 new patients within 7 years (total performed tests: 4,206). Conclusions The presented method and reference intervals enable the quantification of 41 metabolites and the potential diagnosis of up to 25 disorders of PuPy metabolism.

Published
2023
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34. The Role of α-Linolenic Acid and Its Oxylipins in Human Cardiovascular Diseases

Author

Cambiaggi, Lucia, Chakravarty, Akash, Noureddine, Nazek, Hersberger, Martin, and University of Zurich

Subjects
Inorganic Chemistry, 10036 Medical Clinic, Organic Chemistry, 610 Medicine & health, General Medicine, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, Catalysis, Computer Science Applications
Abstract

α-linolenic acid (ALA) is an essential C-18 n-3 polyunsaturated fatty acid (PUFA), which can be elongated to longer n-3 PUFAs, such as eicosapentaenoic acid (EPA). These long-chain n-3 PUFAs have anti-inflammatory and pro-resolution effects either directly or through their oxylipin metabolites. However, there is evidence that the conversion of ALA to the long-chain PUFAs is limited. On the other hand, there is evidence in humans that supplementation of ALA in the diet is associated with an improved lipid profile, a reduction in the inflammatory biomarker C-reactive protein (CRP) and a reduction in cardiovascular diseases (CVDs) and all-cause mortality. Studies investigating the cellular mechanism for these beneficial effects showed that ALA is metabolized to oxylipins through the Lipoxygenase (LOX), the Cyclooxygenase (COX) and the Cytochrome P450 (CYP450) pathways, leading to hydroperoxy-, epoxy-, mono- and dihydroxylated oxylipins. In several mouse and cell models, it has been shown that ALA and some of its oxylipins, including 9- and 13-hydroxy-octadecatrienoic acids (9-HOTrE and 13-HOTrE), have immunomodulating effects. Taken together, the current literature suggests a beneficial role for diets rich in ALA in human CVDs, however, it is not always clear whether the described effects are attributable to ALA, its oxylipins or other substances present in the supplemented diets.

Published
2023
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39. Lipoproteins

Author

Hersberger, Martin, Rohrer, Lucia, von Eckardstein, Arnold, Blau, Nenad, editor, Duran, Marinius, editor, and Gibson, K. Michael, editor

Published
2008
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43. Quantitative profiling of inflammatory and pro-resolving lipid mediators in human adolescents and mouse plasma using UHPLC-MS/MS

Author

Hartling, Ivan, Cremonesi, Alessio, Osuna, Ester, Lou, Phing-How, Lucchinetti, Eliana, Zaugg, Michael, Hersberger, Martin, and University of Zurich

Subjects
0301 basic medicine, Adolescent, Linoleic acid, Clinical Biochemistry, 610 Medicine & health, eicosanoids, LC-MS/MS, lipid mediators, oxylipins, reference value, total parenteral nutrition, 030204 cardiovascular system & hematology, Protectin D1, Pharmacology, Biochemistry, medical, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Tandem Mass Spectrometry, Fatty Acids, Omega-3, Animals, Humans, Maresin, Chromatography, High Pressure Liquid, Inflammation, Chemistry, Biochemistry (medical), General Medicine, Eicosapentaenoic acid, 030104 developmental biology, 10036 Medical Clinic, Docosahexaenoic acid, Eicosanoids, lipids (amino acids, peptides, and proteins), Arachidonic acid, Docosapentaenoic acid, Resolvin
Abstract

Objectives Lipid mediators are bioactive lipids which help regulate inflammation. We aimed to develop an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to quantify 58 pro-inflammatory and pro-resolving lipid mediators in plasma, determine preliminary reference ranges for adolescents, and investigate how total parenteral nutrition (TPN) containing omega-3 polyunsaturated fatty acid (n-3 PUFA) or n-6 PUFA based lipid emulsions influence lipid mediator concentrations in plasma. Methods Lipid mediators were extracted from plasma using SPE and measured using UHPLC-MS/MS. EDTA plasma was collected from healthy adolescents between 13 and 17 years of age to determine preliminary reference ranges and from mice given intravenous TPN for seven days containing either an n-3 PUFA or n-6 PUFA based lipid emulsion. Results We successfully quantified 43 lipid mediators in human plasma with good precision and recovery including several leukotrienes, prostaglandins, resolvins, protectins, maresins, and lipoxins. We found that the addition of methanol to human plasma after blood separation reduces post blood draw increases in 12-hydroxyeicosatetraenoic acid (12-HETE), 12-hydroxyeicosapentaenoic acid (12-HEPE), 12S-hydroxyeicosatrienoic acid (12S-HETrE), 14-hydroxydocosahexaenoic acid (14-HDHA) and thromboxane B2 (TXB2). Compared to the n-6 PUFA based TPN, the n-3 PUFA based TPN increased specialized pro-resolving mediators such as maresin 1 (MaR1), MaR2, protectin D1 (PD1), PDX, and resolvin D5 (RvD5), and decreased inflammatory lipid mediators such as leukotriene B4 (LTB4) and prostaglandin D2 (PGD2). Conclusions Our method provides an accurate and sensitive quantification of 58 lipid mediators from plasma samples, which we used to establish a preliminary reference range for lipid mediators in plasma samples of adolescents; and to show that n-3 PUFA, compared to n-6 PUFA rich TPN, leads to a less inflammatory lipid mediator profile in mice.

Published
2021
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47. Endothelial Barrier Disruption by Lipid Emulsions Containing a High Amount of N3 Fatty Acids (Omegaven) but Not N6 Fatty Acids (Intralipid)

Author

Gueguen, Emilie; https://orcid.org/0000-0002-8187-8792, Morsy, Yasser; https://orcid.org/0000-0001-8234-1877, Scharl, Michael; https://orcid.org/0000-0002-6729-1469, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, Zaugg, Michael; https://orcid.org/0000-0002-2016-8973, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Rogler, Gerhard; https://orcid.org/0000-0002-1733-9188, Wawrzyniak, Marcin; https://orcid.org/0000-0002-6911-8010, Gueguen, Emilie; https://orcid.org/0000-0002-8187-8792, Morsy, Yasser; https://orcid.org/0000-0001-8234-1877, Scharl, Michael; https://orcid.org/0000-0002-6729-1469, Krämer, Stefanie D; https://orcid.org/0000-0002-0426-4340, Zaugg, Michael; https://orcid.org/0000-0002-2016-8973, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Rogler, Gerhard; https://orcid.org/0000-0002-1733-9188, and Wawrzyniak, Marcin; https://orcid.org/0000-0002-6911-8010

Published
2022

48. Reference ranges for the polyethylene glycol (PEG) precipitation activity (%PPA) of eight routine enzyme activities

Author

Bürki, Carmen, Volleberg, Martin, Blomgren, Linnea; https://orcid.org/0000-0003-3833-0227, Froese, Sean; https://orcid.org/0000-0003-1557-3517, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Bürki, Carmen, Volleberg, Martin, Blomgren, Linnea; https://orcid.org/0000-0003-3833-0227, Froese, Sean; https://orcid.org/0000-0003-1557-3517, and Hersberger, Martin; https://orcid.org/0000-0002-4472-329X

Abstract

Macroenzymes are high-molecular weight forms of enzymes whose presence in human sera can lead to non-pathological, elevated enzyme activities, resulting in further unnecessary clinical evaluation. Precipitation with polyethylene glycol (PEG) is an efficient method for removing macroforms from patient samples and can therefore be used for their identification. Cut-offs (99. Percentiles) for the PEG precipitation activity (%PPA) for eight routine enzyme activities were determined on Abbott's Alinity c, namely: AST (61%), ALT (70%), GGT (41%), LDH (45%), lipase (56%), ALP (17%), CK (36%) and PAMY (45%). Two macroforms (PAMY and CK) were then identified by gel filtration chromatography. We suggest that a %PPA above the enzyme-specific cut-off makes the presence of a macroform possible while a %PPA ≥80%, i.e. markedly above the cut-off, makes it very likely for all enzymes.

Published
2022

49. Copeptin Release in Arterial Hypotension and Its Association with Severity of Disease in Critically Ill Children

Author

Baumann, Philipp; https://orcid.org/0000-0002-1244-8131, Gotta, Verena; https://orcid.org/0000-0001-6254-5207, Atkinson, Andrew; https://orcid.org/0000-0001-5834-8315, Deisenberg, Markus, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Roggia, Adam; https://orcid.org/0000-0002-2268-8737, Schmid, Kevin; https://orcid.org/0000-0002-4042-8891, Cannizzaro, Vincenzo; https://orcid.org/0000-0002-5587-160X, Baumann, Philipp; https://orcid.org/0000-0002-1244-8131, Gotta, Verena; https://orcid.org/0000-0001-6254-5207, Atkinson, Andrew; https://orcid.org/0000-0001-5834-8315, Deisenberg, Markus, Hersberger, Martin; https://orcid.org/0000-0002-4472-329X, Roggia, Adam; https://orcid.org/0000-0002-2268-8737, Schmid, Kevin; https://orcid.org/0000-0002-4042-8891, and Cannizzaro, Vincenzo; https://orcid.org/0000-0002-5587-160X

Abstract

Low copeptin levels may indicate inadequate arginine-vasopressin release promoting arterial hypotension, whereas high copeptin concentrations may reflect disease severity. This single-center prospective non-randomized clinical trial analyzed the course of blood copeptin in critically ill normo- and hypotensive children and its association with disease severity. In 164 patients (median age 0.5 years (interquartile range 0.1, 2.9)), the mean copeptin concentration at baseline was 43.5 pmol/L. Though not significantly different after 61 h (primary outcome, mean individual change: −12%, p = 0.36, paired t-test), we detected 1.47-fold higher copeptin concentrations during arterial hypotension when compared to normotension (mixed-effect ANOVA, p = 0.01). In total, 8 out of 34 patients (23.5%) with low copeptin concentrations <10 pmol/L were hypotensive. Copeptin was highest in the adjusted mixed-effect regression analysis within the first day (+20% at 14 h) and decreased significantly at 108 h (−27%) compared to baseline (p = 0.002). Moreover, we found a significant association with vasopressor-inotrope treatment intensity, infancy (1–12 months) and cardiopulmonary bypass (all p ≤ 0.001). In conclusion, high copeptin values were associated with arterial hypotension and severity of disease in critically ill children. This study does not support the hypothesis that low copeptin values might be indicative of arginine-vasopressin deficiency. View Full-Text Keywords: neonate; intensive care; biomarker; antidiuretic hormone; vasopressin; arginine-vasopressin; vasopressor; inotrope; cardiopulmonary bypass

Published
2022

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