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Your search keyword '"Hiradate, Yuuki"' showing total 27 results
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27 results on '"Hiradate, Yuuki"'

20. Neurotensin Enhances Sperm Capacitation and Acrosome Reaction in Mice

Author

Hiradate, Yuuki, primary, Inoue, Hiroki, additional, Kobayashi, Norio, additional, Shirakata, Yoshiki, additional, Suzuki, Yutaka, additional, Gotoh, Aina, additional, Roh, Sang-gun, additional, Uchida, Takafumi, additional, Katoh, Kazuo, additional, Yoshida, Manabu, additional, Sato, Eimei, additional, and Tanemura, Kentaro, additional

Published
2014
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21. Site-specific phosphorylation of Tau protein is associated with deacetylation of microtubules in mouse spermatogenic cells during meiosis

Author

Inoue, Hiroki, primary, Hiradate, Yuuki, additional, Shirakata, Yoshiki, additional, Kanai, Kenta, additional, Kosaka, Keita, additional, Gotoh, Aina, additional, Fukuda, Yasuhiro, additional, Nakai, Yutaka, additional, Uchida, Takafumi, additional, Sato, Eimei, additional, and Tanemura, Kentaro, additional

Published
2014
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26. Localization and quantitative analysis of Cx43 in porcine oocytes during in vitro maturation.

Author

Lee S, Hiradate Y, Hoshino Y, Ko YG, Tanemura K, and Sato E

Subjects
Animals, Blotting, Western, Cell Membrane metabolism, Cells, Cultured, Cumulus Cells cytology, Female, Immunohistochemistry, Swine, Time Factors, Connexin 43 metabolism, Cumulus Cells metabolism, In Vitro Oocyte Maturation Techniques methods, Oocytes metabolism
Abstract

Many studies of the main gap junction protein, Cx43, have been conducted in porcine oocyte research, but they have been limited to investigations of cumulus-oocyte complexes (COCs). In this study, we verified Cx43 not in COCs, but in porcine oocytes during maturation, and conducted a quantitative time course analysis. The location and dynamics of Cx43 were examined by immunocytochemistry and western blotting, respectively. COCs were cultured in NCSU23 medium and processed for immunocytochemistry and western blotting at 0, 14, 28, and 42 h after denuding. A Cx43 signal was detected on oolemmas, transzonal projections and the surface of zona pellucidae. Western blotting showed that Cx43 band density increased from 0 to 14 h, and gradually decreased thereafter. Our results clarified that Cx43 is localized in the ooplasmic membrane through zona pellucidae and its level changes over time during culture in porcine oocytes.

Published
2016
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27. Extracellular ATP and dibutyryl cAMP enhance the freezability of rat epididymal sperm.

Author

Yamashiro H, Toyomizu M, Toyama N, Aono N, Sakurai M, Hiradate Y, Yokoo M, Moisyadi S, and Sato E

Subjects
Animals, Cryopreservation methods, Cryoprotective Agents, Embryo Culture Techniques veterinary, Female, Fertilization in Vitro methods, Fertilization in Vitro veterinary, Insemination, Artificial methods, Insemination, Artificial veterinary, Ionomycin pharmacology, Ionophores pharmacology, Male, Oxygen Consumption, Pregnancy, Rats, Rats, Wistar, Semen Preservation methods, Sperm Motility, Adenosine Triphosphate administration & dosage, Bucladesine administration & dosage, Cryopreservation veterinary, Epididymis cytology, Semen Preservation veterinary, Spermatozoa physiology
Abstract

We studied the effects of ATP, ionomycin, and dibutyryl cAMP (dbcAMP) on the motility, freezability, and oxygen consumption of rat epididymal sperm. In vitro fertilization and intrauterine insemination were performed by using frozen-thawed rat sperm. Frozen-thawed sperm diluted in raffinose-modified Krebs-Ringer bicarbonate solution-egg yolk extender containing 1.85 mM ATP and 100 microM dbcAMP exhibited considerably higher motility and viability than sperm diluted in dbcAMP-free extender. Addition of ionomycin and dbcAMP to ATP-containing extenders did not alter the oxygen consumption rate of sperm, suggesting that extracellular ionomycin and dbcAMP are not involved in the mobilization of mitochondrial energy substrates in sperm. Further, high rates of pronucleus formation and progression to the blastocyst stage were observed in embryos produced by the fertilization of oocytes with fresh sperm in an in vitro fertilization medium supplemented with ATP and dbcAMP. Oocytes were not penetrated by frozen-thawed sperm when cocultured with cumulus-oocyte complexes in a medium without ATP and dbcAMP. In contrast, cryopreserved sperm penetrated oocytes when the gametes were cultured in an ATP- and dbcAMP-containing medium, and the resultant embryos formed blastocysts. Our results show that the dilution of rat sperm in raffinose-modified Krebs-Ringer bicarbonate solution-egg yolk extender supplemented with ATP and dbcAMP prior to sperm cryopreservation enhances the freezability of the cryopreserved sperm. Furthermore, the in vitro fertilization medium we developed effectively supports the production of embryos from both fresh and cryopreserved rat sperm.

Published
2010

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