25 results on '"Hiromi Nagaoka"'
Search Results
2. Whole-Genome Sequencing of Shiga Toxin–Producing Escherichia coli OX18 from a Fatal Hemolytic Uremic Syndrome Case
- Author
-
Kenichi Lee, Atsushi Iguchi, Kazuhiro Uda, Sohshi Matsumura, Isao Miyairi, Kenji Ishikura, Makoto Ohnishi, Junji Seto, Kanako Ishikawa, Noriko Konishi, Hiromi Obata, Ichiro Furukawa, Hiromi Nagaoka, Hirotaka Morinushi, Natsuki Hama, Ryohei Nomoto, Hiroshi Nakajima, Hideaki Kariya, Mitsuhiro Hamasaki, and Sunao Iyoda
- Subjects
Bacteria ,E. coli ,Og-typing ,OX18 ,Shiga toxin–producing Escherichia coli ,foodborne pathogens ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We report a fatal case of hemolytic uremic syndrome with urinary tract infection in Japan caused by Shiga toxin–producing Escherichia coli. We genotypically identified the isolate as OX18:H2. Whole-genome sequencing revealed 3 potentially pathogenic lineages (OX18:H2, H19, and H34) that have been continuously isolated in Japan.
- Published
- 2021
- Full Text
- View/download PDF
3. Isolation Methods of Escherichia albertii from Food and Environment Water, and the Analysis of Isolates.
- Author
-
Sakura ARAI, Akito MIZOKOSHI, Miyuki SAEKI, Keiko KIMATA, Keita YANAGIMOTO, Seiya HARADA, Satoko YAMAYA, Yuki TOKOI, Tomoko FUKUDOME, Hiromi NAGAOKA, Kaori YAMADA, Natsuki HAMA, Takuya YAMANAKA, Akihiko TSUCHIYA, Yukiko ASANO, Yukiko NAKAMURA, Norihisa MATSUNAGA, Taketoshi TAKARA, Takayuki KONNO, and Noriko KONISHI
- Abstract
Escherichia albertii is an emerging enteropathogen and its distribution in various foods and environmental samples has been reported in many regions around the world. In this study, we aimed to identify effective isolation and detection methods for E. albertii in various foods and environmental water samples. E. albertii-specific polymerase chain reaction (PCR) was positive in chicken, oyster, river water, and wastewater samples, and E. albertii was isolated from these PCR-positive samples except the wastewater sample. E. albertii was not isolated from any of the samples without screening PCR; therefore, PCR is useful for the detection and isolation of E. albertii in foods and environmental water samples. The effect of two-step enrichment with four kinds of selective enrichment broth was compared with cycle threshold (Ct) values of the E. albertii-specific real-time PCR assay and the isolation results. The Ct values in three out of five samples were lower in the second enriched culture than those of the first enriched culture, and E. albertii was isolated from enriched cultures showed Ct values <25. These results suggest that the population of E. albertii in these three samples increased in the second enriched culture compared with the first enriched culture, and isolating E. albertii from an enriched culture showing Ct values <25 is an efficient method. Genetic analysis was performed to E. albertii isolates from food, environmental water, and human fecal samples, and all the isolates possessed eae, and isolates from chicken, pork, and river water samples showed the same EAOg type as E. albertii isolated from human fecal samples. Therefore, it was suggested that a continuous attention should be paid to E. albertii in food and environment. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Development of a Novel Real-Time Polymerase Chain Reaction Assay to DetectEscherichia albertiiin Chicken Meat
- Author
-
Sakura Arai, Tadasuke Ooka, Mizuha Shibata, Yuhki Nagai, Yuki Tokoi, Hiromi Nagaoka, Rika Maeda, Akihiko Tsuchiya, Yuka Kojima, Kenji Ohya, Takahiro Ohnishi, Noriko Konishi, Kayoko Ohtsuka, and Yukiko Hara-Kudo
- Subjects
Animal Science and Zoology ,Applied Microbiology and Biotechnology ,Microbiology ,Food Science - Published
- 2022
- Full Text
- View/download PDF
5. Antimicrobial Resistance of Salmonella Isolated from the Contents of Chicken Cecum in Shizuoka Prefecture
- Author
-
Shiro MIZUMOTO, Kana SUZUKI, Kai OKOSHI, Aya OGAWA, Rikiya KUGE, Hirotaka MORINUSHI, Hiromi NAGAOKA, and Koichi MURAKAMI
- Published
- 2021
- Full Text
- View/download PDF
6. Whole-Genome Sequencing of Shiga Toxin–Producing Escherichia coli OX18 from a Fatal Hemolytic Uremic Syndrome Case
- Author
-
Kanako Ishikawa, Sohshi Matsumura, Kazuhiro Uda, Ken-ichi Lee, Mitsuhiro Hamasaki, Isao Miyairi, Natsuki Hama, Makoto Ohnishi, Ryohei Nomoto, Noriko Konishi, Junji Seto, Hiromi Obata, Sunao Iyoda, Ichiro Furukawa, Hirotaka Morinushi, Kenji Ishikura, Hideaki Kariya, Atsushi Iguchi, Hiromi Nagaoka, and Hiroshi Nakajima
- Subjects
Microbiology (medical) ,Epidemiology ,Shiga toxin–producing Escherichia coli ,030231 tropical medicine ,Infectious and parasitic diseases ,RC109-216 ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Japan ,medicine ,Humans ,030212 general & internal medicine ,foodborne pathogens ,Shiga toxin-producing Escherichia coli ,Escherichia coli ,Escherichia coli Infections ,Original Research ,Whole genome sequencing ,Whole-Genome Sequencing of Shiga Toxin–Producing Escherichia coli OX18 from a Fatal Hemolytic Uremic Syndrome Case ,Shiga-Toxigenic Escherichia coli ,Whole Genome Sequencing ,Bacteria ,biology ,enteric infections ,Dispatch ,E. coli ,biology.organism_classification ,OX18 ,Og-typing ,STEC ,food safety ,Infectious Diseases ,whole-genome sequencing ,Hemolytic-Uremic Syndrome ,Medicine - Abstract
We report a fatal case of hemolytic uremic syndrome with urinary tract infection in Japan caused by Shiga toxin–producing Escherichia coli. We genotypically identified the isolate as OX18:H2. Whole-genome sequencing revealed 3 potentially pathogenic lineages (OX18:H2, H19, and H34) that have been continuously isolated in Japan.
- Published
- 2021
- Full Text
- View/download PDF
7. Evaluating Methods for Detecting Escherichia albertii in Chicken Meat
- Author
-
Takatoshi Takara, Kenji Ohya, Yukiko Asano, Yuki Tokoi, Takayuki Konno, Yukiko Hara-Kudo, Hiromi Nagaoka, Hiroyuki Maruyama, Noriko Konishi, Kayoko Ohtsuka, Hiroko Uchiyama, and Sakura Arai
- Subjects
Escherichia ,Meat ,food.ingredient ,Rhamnose ,Biology ,Microbiology ,Enrichment culture ,Escherichia albertii ,03 medical and health sciences ,chemistry.chemical_compound ,food ,Japan ,Animals ,Agar ,Food science ,Lactose ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,biology.organism_classification ,Culture Media ,chemistry ,Food Microbiology ,MacConkey agar ,Chickens ,Nested polymerase chain reaction ,Bacteria ,Food Science - Abstract
Escherichia albertii is an emerging foodborne pathogen. The source of the E. albertii infection in most foodborne outbreaks is unknown because E. albertii is difficult to isolate from suspected food or water. E. albertii has a broad host range among birds and can be isolated from chicken meat. In this study, PCR assay, enrichment, and isolation conditions for detecting E. albertii in chicken meat were evaluated. The growth of 47 E. albertii strains isolated in Japan between 1994 and 2018 and a type strain was evaluated in modified EC broth (mEC) and mEC supplemented with novobiocin (NmEC) and on media containing carbohydrates. The enzyme used for the nested PCR, the enrichment conditions, the most-probable-number (MPN) method, and agar media were also evaluated with chicken meat. To distinguish E. albertii from presumptive non-E. albertii bacteria, desoxycholate hydrogen sulfide lactose agar (DHL), MacConkey agar (MAC), and these agars supplemented with rhamnose and xylose (RX-DHL and RX-MAC, respectively) were used. All E. albertii strains grew in mEC and NmEC at both 36 and 42°C and did not utilize rhamnose, sucrose, or xylose. Both the first and nested PCRs with TaKaRa Ex Taq, which was 10 to 100 times more active than the other enzymes, produced positive results in enrichment culture of 25 g of chicken meat inoculated with >20 CFU of E. albertii and incubated in mEC and NmEC at 42°C for 22 ± 2 h. Thus, the first PCR was sensitive enough to detect E. albertii in chicken meat. The MPN values in mEC and NmEC were 0.5- and 2.3-fold higher than the original inoculated bacterial levels, respectively. E. albertii in chicken meat was more efficiently isolated with enrichment in NmEC (70.1 to 100%) and plating onto RX-DHL (85.4%) and RX-MAC (100%) compared with enrichment in mEC (53.5 to 83.3%) and plating onto DHL (70.1%) and MAC (92.4%). Thus, optimized conditions for the surveillance of E. albertii contamination in food and investigations of E. albertii outbreaks, including the infectious dose, were clarified. HIGHLIGHTS
- Published
- 2021
- Full Text
- View/download PDF
8. Antimicrobial Drug-resistance Profile of Vibrio Parahaemolyticus isolated from Japanese Horse Mackerel (Trachurus Japonicus)
- Author
-
Keiichi Goto, Hiromi Nagaoka, Hideki Suzuki, Shiro Mizumoto, Tasturo Nishino, Hirotaka Morinushi, and Shigeki Yamamoto
- Subjects
Serotype ,Antibiotic resistance ,biology ,Trachurus japonicus ,Vibrio parahaemolyticus ,Environmental exposure ,Antimicrobial ,biology.organism_classification ,Horse mackerel ,Bacteria ,Microbiology - Abstract
This study aimed at investigating antimicrobial resistance (AMR) profile of Vibrio parahaemolyticus (V. parahaemolyticus). The bacteria were isolated from wild-caught and farmed Japanese horse mackerel (Trachurus japonicus), and examined for the antimicrobial drug resistance. Furthermore, the serotype, and the genes of thermostable direct hemolysin (tdh) and cholera toxin transcriptional activator (toxR) of the isolates were investigated by using a serotype testing kit and PCR method. Eighty-eight and 126 V. parahaemolyticus strains were isolated from wild-caught and farmed Japanese horse mackerel, respectively. Ten and 18 distinct serotypes were detected from wild-caught and farmed Japanese horse mackerel. All strains were negative for tdh genes but positive for toxR genes. Resistances to ampicillin (ABP) and to both ABP and fosfomycin (FOM) were observed in 54 and 23 strains from the wild-caught fish, while those resistant strains from farm fish were 112 and 7 strains. Multidrug-resistance to three or four drugs including ABP was observed in one or two strains from the wild-caught fish. These results strongly suggest that the environmental exposure of antimicrobial drugs results in the spread of resistant genes in Japanese horse mackerel. This study highlights the need for monitoring the spread of resistance genes to the human intestinal flora as well as to other bacteria in the environment.
- Published
- 2021
- Full Text
- View/download PDF
9. Draft Genome Sequence of Campylobacter jejuni ST-508 Strain Shizu21005, Isolated from an Asymptomatic Food Handler in Japan, 2021
- Author
-
Aya Ogawa, Hiromi Nagaoka, and Hiroshi Asakura
- Subjects
Immunology and Microbiology (miscellaneous) ,Genetics ,Molecular Biology - Abstract
Here, we report a draft genome sequence of Campylobacter jejuni strain Shizu21005, isolated from a food handler with no symptoms in Japan on March 2021. Its genome size was 1,656,785 bp, with 2 rRNAs, 35 tRNAs, and a coverage of 330×.
- Published
- 2022
- Full Text
- View/download PDF
10. Antimicrobial Drug-resistance Profile of
- Author
-
Tasturo, Nishino, Hideki, Suzuki, Shiro, Mizumoto, Hirotaka, Morinushi, Hiromi, Nagaoka, Keiichi, Goto, and Shigeki, Yamamoto
- Subjects
antimicrobial drug resistance ,Japanese horse mackerel (Trachurus japonicus) ,Short Communication ,Vibrio parahaemolyticus - Abstract
This study aimed at investigating antimicrobial resistance (AMR) profile of Vibrio parahaemolyticus (V. parahaemolyticus). The bacteria were isolated from wild-caught and farmed Japanese horse mackerel (Trachurus japonicus), and examined for the antimicrobial drug resistance. Furthermore, the serotype, and the genes of thermostable direct hemolysin (tdh) and cholera toxin transcriptional activator (toxR) of the isolates were investigated by using a serotype testing kit and PCR method. Eighty-eight and 126 V. parahaemolyticus strains were isolated from wild-caught and farmed Japanese horse mackerel, respectively. Ten and 18 distinct serotypes were detected from wild-caught and farmed Japanese horse mackerel. All strains were negative for tdh genes but positive for toxR genes. Resistances to ampicillin (ABP) and to both ABP and fosfomycin (FOM) were observed in 54 and 23 strains from the wild-caught fish, while those resistant strains from farm fish were 112 and 7 strains. Multidrug-resistance to three or four drugs including ABP was observed in one or two strains from the wild-caught fish. These results strongly suggest that the environmental exposure of antimicrobial drugs results in the spread of resistant genes in Japanese horse mackerel. This study highlights the need for monitoring the spread of resistance genes to the human intestinal flora as well as to other bacteria in the environment.
- Published
- 2021
11. Contrasting Results from Two Commercial Kits Testing for the Presence of Clostridium perfringens Enterotoxin in Feces from Norovirus-Infected Human Patients
- Author
-
Asako Nakamura, Arimi Nakamoto, Yuki Carle, Shuji Fujimoto, Shinichiro Hirai, Yoshiyuki Aihara, Hiromi Nagaoka, Hiroaki Shigemura, Taisei Ishioka, Koichi Murakami, Akiko Kubomura, Kazunori Oishi, Hirokazu Kimura, and Takumi Motoya
- Subjects
Adult ,Diarrhea ,Male ,Adolescent ,Enzyme-Linked Immunosorbent Assay ,Enterotoxin ,Biology ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,Microbiology ,Elisa kit ,Enterotoxins ,Feces ,Young Adult ,medicine ,Humans ,Child ,Aged ,Caliciviridae Infections ,Aged, 80 and over ,Clostridium perfringens ,Middle Aged ,Latex fixation test ,Gastrointestinal Microbiome ,Toxin detection ,Norovirus ,Female ,medicine.symptom ,Latex Fixation Tests - Abstract
Background Detection of Clostridium perfringens enterotoxin (CPE) is critical for disease surveillance; however, commercial testing kits produce contrasting results. Methods We examined the cause of the differing results from a reversed passive latex agglutination (RPLA) assay (PET-RPLA Toxin Detection Kit) and an enzyme-linked immunosorbent assay (C. perfringens Enterotoxin ELISA Kit) using 73 human norovirus-positive fecal samples from gastroenteritis patients across 22 episodes in Japan. Results CPE was detected in 39/73 samples using the RPLA method; however, ELISA-based examination of 10 RPLA-positive samples produced negative results. Moreover, cpe was not detected in any of the RPLA-positive (n = 32) or -negative (n = 5) samples, and C. perfringens was only isolated from one RPLA-positive sample. Conclusions An ELISA-based testing approach may be more reliable than RPLA assays for CPE detection from human fecal samples. These findings may also be applicable to the detection of other foodborne diseases.
- Published
- 2020
12. Coinfection with Human Norovirus and Escherichia coli O25:H4 Harboring Two Chromosomal blaCTX-M-14 Genes in a Foodborne Norovirus Outbreak in Shizuoka Prefecture, Japan
- Author
-
Takashi Kanda, Hiroaki Shigemura, Naoto Takahashi, Michiko Asanuma, Hiromi Nagaoka, Makoto Kuroda, Fumie Suzuki, Shiro Mizumoto, Kana Suzuki, Satowa Suzuki, Kai Ohkoshi, Shinichiro Hirai, Hirokazu Kimura, Mizuha Mochizuki, Takaharu Maehata, Motoi Suzuki, Aya Ogawa, Tsuyoshi Sekizuka, Koichi Murakami, Taisei Ishioka, and Hirotaka Morinushi
- Subjects
Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Microbiology ,Genome ,Chromosomes ,beta-Lactamases ,Disease Outbreaks ,03 medical and health sciences ,Plasmid ,Japan ,medicine ,Escherichia coli ,Humans ,Escherichia coli Infections ,030304 developmental biology ,0303 health sciences ,030306 microbiology ,Coinfection ,Norovirus ,Outbreak ,medicine.disease ,Subtyping ,Anti-Bacterial Agents ,Multilocus sequence typing ,Food Science - Abstract
Hospital-acquired infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are a global problem. Healthy people can carry ESBL-producing E. coli in the intestines; thus, E. coli from healthy people can potentially cause hospital-acquired infections. Therefore, the transmission routes of ESBL-producing E. coli from healthy persons should be determined. A foodborne outbreak of human norovirus (HuNoV) GII occurred at a restaurant in Shizuoka, Japan, in 2018. E. coli O25:H4 was isolated from some of the HuNoV-infected customers. Pulsed-field gel electrophoresis showed that these E. coli O25:H4 strains originated from one clone. Because the only epidemiological link among the customers was eating food from this restaurant, the customers were concurrently infected with E. coli O25:H4 and HuNoV GII via the restaurant food. Whole genome analysis revealed that the E. coli O25:H4 strains possessed genes for regulating intracellular iron and expressing the flagellum and flagella. Extraintestinal pathogenic E. coli often express these genes on the chromosome. Additionally, the E. coli O25:H4 strains had plasmids harboring nine antimicrobial resistance genes. These strains harbored ESBL-encoding blaCTX-M-14 genes on two loci of the chromosome and had higher ESBL activity. Multilocus sequence typing and fimH subtyping revealed that the E. coli O25:H4 strains from the outbreak belonged to the subclonal group, ST131-fimH30R, which has been driving ESBL epidemics in Japan. Because the E. coli O25:H4 strains isolated in the outbreak belonged to a subclonal group spreading in Japan, foods contaminated with ESBL-producing E. coli might contribute to spreading these strains among healthy persons. The isolated E. coli O25:H4 strains produced ESBL and contained plasmids with multiple antimicrobial resistance genes, which may make it difficult to select antimicrobials for treating extraintestinal infections caused by these strains. HIGHLIGHTS
- Published
- 2020
13. Coinfection with Human Norovirus and Escherichia coli O25:H4 Harboring Two Chromosomal blaCTX-M-14 Genes in a Foodborne Norovirus Outbreak in Shizuoka Prefecture, Japan.
- Author
-
HIROMI NAGAOKA, SHINICHIRO HIRAI, HIROTAKA MORINUSHI, SHIRO MIZUMOTO, KANA SUZUKI, HIROAKI SHIGEMURA, NAOTO TAKAHASHI, FUMIE SUZUKI, MIZUHA MOCHIZUKI, MICHIKO ASANUMA, TAKAHARU MAEHATA, AYA OGAWA, KAI OHKOSHI, TSUYOSHI SEKIZUKA, TAISEI ISHIOKA, SATOWA SUZUKI, HIROKAZU KIMURA, MAKOTO KURODA, MOTOI SUZUKI, and KOICHI MURAKAMI
- Abstract
Hospital-acquired infections caused by extended-spectrum β-lactamase (ESBL)–producing Escherichia coli are a global problem. Healthy people can carry ESBL-producing E. coli in the intestines; thus, E. coli from healthy people can potentially cause hospital-acquired infections. Therefore, the transmission routes of ESBL-producing E. coli from healthy persons should be determined. A foodborne outbreak of human norovirus (HuNoV) GII occurred at a restaurant in Shizuoka, Japan, in 2018. E. coli O25:H4 was isolated from some of the HuNoV-infected customers. Pulsed-field gel electrophoresis showed that these E. coli O25:H4 strains originated from one clone. Because the only epidemiological link among the customers was eating food from this restaurant, the customers were concurrently infected with E. coli O25:H4 and HuNoV GII via the restaurant food. Whole genome analysis revealed that the E. coli O25:H4 strains possessed genes for regulating intracellular iron and expressing the flagellum and flagella. Extraintestinal pathogenic E. coli often express these genes on the chromosome. Additionally, the E. coli O25:H4 strains had plasmids harboring nine antimicrobial resistance genes. These strains harbored ESBL-encoding blaCTX-M-14 genes on two loci of the chromosome and had higher ESBL activity. Multilocus sequence typing and fimH subtyping revealed that the E. coli O25:H4 strains from the outbreak belonged to the subclonal group, ST131-fimH30R, which has been driving ESBL epidemics in Japan. Because the E. coli O25:H4 strains isolated in the outbreak belonged to a subclonal group spreading in Japan, foods contaminated with ESBL-producing E. coli might contribute to spreading these strains among healthy persons. The isolated E. coli O25:H4 strains produced ESBL and contained plasmids with multiple antimicrobial resistance genes, which may make it difficult to select antimicrobials for treating extraintestinal infections caused by these strains. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
14. Contrasting Results from Two Commercial Kits Testing for the Presence of Clostridium perfringens Enterotoxin in Feces from Norovirus-Infected Human Patients.
- Author
-
Taisei Ishioka, Yoshiyuki Aihara, Yuki Carle, Hiroaki Shigemura, Akiko Kubomura, Takumi Motoya, Arimi Nakamoto, Asako Nakamura, Shuji Fujimoto, Shinichiro Hirai, Kazunori Oishi, Hiromi Nagaoka, Hirokazu Kimura, and Koichi Murakami
- Subjects
NOROVIRUS diseases ,CLOSTRIDIUM perfringens ,ENTEROTOXINS ,FOODBORNE diseases ,ENZYME-linked immunosorbent assay ,FECES - Abstract
Background: Detection of Clostridium perfringens enterotoxin (CPE) is critical for disease surveillance; however, commercial testing kits produce contrasting results. Methods: We examined the cause of the differing results from a reversed passive latex agglutination (RPLA) assay (PET-RPLA Toxin Detection Kit) and an enzyme-linked immunosorbent assay (C. perfringens Enterotoxin ELISA Kit) using 73 human norovirus-positive fecal samples from gastroenteritis patients across 22 episodes in Japan. Results: CPE was detected in 39/73 samples using the RPLA method; however, ELISA-based examination of 10 RPLA-positive samples produced negative results. Moreover, cpe was not detected in any of the RPLA-positive (n = 32) or -negative (n = 5) samples, and C. perfringens was only isolated from one RPLA-positive sample. Conclusions: An ELISA-based testing approach may be more reliable than RPLA assays for CPE detection from human fecal samples. These findings may also be applicable to the detection of other foodborne diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
15. Prevalence and Clinical Characterization of Coxiella burnetii Infection in Patients with Protracted Low-grade Fever
- Author
-
Yukiko Ishikawa, Masato Akiyama, Yasuhiro Hakamata, and Hiromi Nagaoka
- Subjects
Adult ,DNA, Bacterial ,Male ,medicine.medical_specialty ,Fever ,Population ,Q fever ,Asymptomatic ,Gastroenterology ,Serology ,Low-grade fever ,Japan ,Seroepidemiologic Studies ,Internal medicine ,Prevalence ,Humans ,Medicine ,Endocarditis ,Prospective Studies ,education ,education.field_of_study ,biology ,business.industry ,General Medicine ,Middle Aged ,Coxiella burnetii ,biology.organism_classification ,medicine.disease ,Antibodies, Bacterial ,Virology ,Titer ,Chronic Disease ,Female ,medicine.symptom ,Q Fever ,business - Abstract
We report here a persistent form of Coxiella burnetii infection. There have been no prospective surveys of chronic C. burnetii infection reported in Japan. Until recently, it was not possible to distinguish between previous and current infection with serological tests for antibody to C. burnetii. The nested PCR method, however, allows us to appreciate the current infection by detecting C. burnetii DNA with high sensitivity. Inoculation method using an A/J mouse was performed to confirm the viability of C. burnetii. To obtain an approximation of the prevalence of C. burnetii infection in the general population, we evaluated a random sample of patients with symptoms of continuous low-grade fever for one month or more. Analysis of 54 subjects with protracted debility and fatigue symptoms identified 13 subjects as carriers of C. burnetii (24.1%). There were no significant differences in age, C-reactive protein levels (0.69 +/- 1.19 mg/dl), white blood cell counts (6,089 +/- 2,189/microliter), eosinophil (3.4 +/- 3.6%) between the patients with C. burnetii infection and infection-free subjects. All thirteen patients had experienced protracted low-grade fever (up to 37.5 degrees C) for four months to seven years (30.5 +/- 27.7 months). Transthoracic echocardiography showed no evidence of endocarditis, or echosonography revealed no abnormal findings in the liver or kidneys. Although domestic animals constitute an important reservoir of C. burnetii, only two of the positive subjects had direct contact with them and none of the positive subjects were occupationally exposed to farm animals or common sources of infection. None had a history of hospitalizations for pneumonia or hepatic disease. Interestingly, five of the thirteen patients had a history of consulting a psychiatrist, and furthermore, one had a history of several admissions in a psychiatric hospital due to chronic fatigue symptoms. Ten of the patients had a high IgE titer (> 295 IU/ml), which shows a higher prevalence than in patients without C. burnetii (76.9%: 22.0%, P = 0.001). Four of them had markedly elevated IgE levels, in excess of 2,000 IU/ml. The mean value of IgE was higher in the patients with C. burnetii infection than in infection-free subjects (1,388 +/- 1,706: 533 +/- 913 IU/ml, p < 0.045). Two subjects were rheumatoid factor positive and another three had autoimmune thyroiditis. Twelve of the 13 subjects provided written informed consent for treatment with minocycline (200 mg/day). One month later, all subject became asymptomatic and apyretic (37.1 +/- 0.43 degrees C to 36.7 +/- 0.56 degrees C; p < 0.025), and nested PCR did not identify C. burnetii DNA in serum samples. It should be noted that persistent symptoms including low-grade fever were observed for two weeks after the start of medication. Furthermore, three patients had persistent symptoms, and DNA detection by the nested PCR method became positive in all three patients within a few months.
- Published
- 2002
- Full Text
- View/download PDF
16. Isolation ofCoxiella burnetiifrom Children with Influenza-Like Symptoms in Japan
- Author
-
To Ho, Hiroshi Hattori, Sousuke Akahane, Hideto Fukushi, Masaaki Sugieda, Tomohiro Nishio, Hiromi Nagaoka, Masato Akiyama, and Katsuya Hirai
- Subjects
Immunology ,Q fever ,Microbiology ,Giemsa stain ,Rickettsiaceae ,Cell Line ,Dogs ,Japan ,Antigen ,Virology ,Influenza, Human ,medicine ,Animals ,Humans ,Child ,biology ,bacterial infections and mycoses ,medicine.disease ,Coxiella burnetii ,biology.organism_classification ,Antibodies, Bacterial ,Rickettsiosis ,Influenza A virus ,Acute Disease ,biology.protein ,bacteria ,Antibody ,Q Fever ,Rickettsiales - Abstract
The prevalence of Coxiella burnetii antibodies was investigated by indirect immunofluorescence (IF) test in 55 paired sera (acute and convalescent phases) of school children who had influenza-like symptoms. Of the convalescent serum samples examined, 18 (32.7%) sera reacted positively to phase II antigen of C. burnetii. Coxiella-like organism was isolated from the sera of 13 children after injection of the 18 acute phase sera into mice. The organism was identified as C. burnetii by Giemsa staining and the IF antigen test of mouse spleen smears, the polymerase chain reaction (PCR) method, electron microscopic observations of the mouse spleen cells, and the IF antibody test of mouse sera. This is the first report of isolation of C. burnetii from serum specimens of children having influenza-like symptoms. The evidence that C. burnetii was isolated from people indigenous to Japan at a considerably high incidence suggested that C. burnetii may be widespread as a cause of influenza-like symptoms in Japan.
- Published
- 1996
- Full Text
- View/download PDF
17. Comparison of Japanese isolates of Coxiella burnetii by PCR-RFLP and sequence analysis
- Author
-
Katsuya Hirai, Masako Andoh, Tsuyoshi Yamaguchi, Hiromi Nagaoka, and Hideto Fukushi
- Subjects
DNA, Bacterial ,Sequence analysis ,Immunology ,Molecular Sequence Data ,Q fever ,Microbiology ,Polymerase Chain Reaction ,Virology ,Genetic variation ,medicine ,Animals ,Humans ,Point Mutation ,Amino Acid Sequence ,Peptide sequence ,Gene ,Genetics ,biology ,Base Sequence ,Nucleic acid sequence ,bacterial infections and mycoses ,Coxiella burnetii ,biology.organism_classification ,medicine.disease ,Isocitrate Dehydrogenase ,bacteria ,Restriction fragment length polymorphism ,Q Fever ,Sequence Alignment - Abstract
The genetic variation of Japanese isolates of Coxiella burnetii, the agent of Q fever, was found for the first time. Forty-nine out of 72 isolates had the chronic pattern of the isocitrate hydrogenase gene. Sequence analysis revealed that the isolates have a specific nucleotide sequence. The putative amino acid sequence was the same as that of chronic reference strains. These results suggest the variation of C. burnetii isolates in Japan.
- Published
- 2004
18. [Serological cross-reaction among Bartonella henselae, Chlamydia pneumoniae and Coxiella burnetii by indirect fluorescence antibody method]
- Author
-
Hiromi Nagaoka, Akiko Umeda, Hidechika Iino, Kazunobu Ouchi, Chizuru Ishida, Kumiko Tsujino, Masato Tsukahara, Kyoko Murakami, and Hidehiro Tsuneoka
- Subjects
Q fever ,Cross Reactions ,Serology ,Antigen ,Medicine ,Humans ,Fluorescent Antibody Technique, Indirect ,Chlamydophila Infections ,Bartonella henselae ,biology ,business.industry ,Antibody titer ,Cat-Scratch Disease ,Cat-scratch disease ,General Medicine ,Chlamydophila pneumoniae ,bacterial infections and mycoses ,medicine.disease ,Coxiella burnetii ,biology.organism_classification ,Virology ,biology.protein ,bacteria ,Antibody ,business ,Q Fever - Abstract
We studied the serological cross-reactions among Bartonella henselae, Chlamydia pneumoniae and Coxiella burnetii by indirect fluorescence antibody (IFA) method, using sera from 8 patients with cat scratch disease (CSD), 13 patients with C. pneumoniae infection and 12 patients with acute Q fever. B. henselae IgG antibody was negative in 13 patients with C. pneumoniae infection, and was positive in 3 (titers being 1:64) of 12 patients with Q fever, whereas B. henselae IgM antibody was negative in all the patients with C. pneumoniae infection or Q fever. C. burnetii IgG antibody was removed by absorption of these 3 sera with C. burnetii antigens, whereas B. henselae IgG antibody did not change. C. pneumoniae IgG antibody was positive in 3 (titers being 1:125 in two, 1:32 in one) of 8 patients with CSD. Both C. pneumoniae and B. henselae IgG antibody titers were significantly reduced by absorption of these 3 sera with B. henselae antigens. C. burnetii IgG or IgM antibodies were negative in all patients with CSD. In conclusion, no serological cross-reaction between B. henselae and C. burnetii was observed. On the other hand. B. henselae IgG antibody cross-reacted to C. pneumoniae antigens, whereas C. pneumoniae IgG antibody did not cross-react to B. henselae antigens. Our findings suggest that determination of B. henselae IgG or IgM antibodies were not influenced by C. pneumoniae and C. burnetii antigens.
- Published
- 2001
19. Intractable Q fever treated with recombinant gamma interferon
- Author
-
Hiromi Nagaoka, Yutaka Morisawa, Hiroshi Wakiguchi, Takanobu Kurashige, and Tomoki Takechi
- Subjects
Microbiology (medical) ,Male ,medicine.drug_class ,Antibiotics ,Q fever ,macromolecular substances ,Asymptomatic ,Interferon-gamma ,Medicine ,Humans ,Interferon gamma ,Antibacterial agent ,biology ,business.industry ,Minocycline ,bacterial infections and mycoses ,medicine.disease ,Coxiella burnetii ,biology.organism_classification ,Virology ,Infectious Diseases ,Rickettsiosis ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Immunology ,bacteria ,medicine.symptom ,business ,Q Fever ,medicine.drug - Abstract
A 3-year-old boy with Q fever received several kinds of antibiotics including minocycline, but spiking fever and positive PCR of Coxiella burnetii continued for several months. He became asymptomatic and his abnormal laboratory data normalized after the administration of gamma interferon three times a week.
- Published
- 2001
20. Coxiella burnetii lymphadenitis: a possible fever focus in acute Q fever
- Author
-
Toshiya Shinohara, Yukio Sakiyama, Tohru Watanabe, Yukihiro Kusunoki, Akihiko Miyanoshita, Hiromi Nagaoka, and Tadashi Ariga
- Subjects
Male ,Focus (computing) ,biology ,Adolescent ,business.industry ,Q fever ,Coxiella burnetii ,biology.organism_classification ,medicine.disease ,Virology ,Lymphadenitis ,Pediatrics, Perinatology and Child Health ,Immunology ,medicine ,Humans ,Lymph Nodes ,business ,Q Fever ,Radionuclide Imaging ,Immunocompetence ,Neck - Published
- 2001
21. [Epidemiological analysis of influenza B virus belonging to B/Victoria/2/87 lineage isolated in off-season of 1998 and late epidemic season in Shizuoka Prefecture]
- Author
-
Hideki Miyamoto, Reiko Nerome, Hiromi Nagaoka, Masaaki Sugieda, Yoshinobu Miwa, Keiji Sahara, Setsuko Nakajima, and Masato Akiyama
- Subjects
medicine.medical_specialty ,Lineage (genetic) ,biology ,Epidemic season ,viruses ,Outbreak ,General Medicine ,Virology ,H5N1 genetic structure ,Virus ,Disease Outbreaks ,Titer ,Influenza B virus ,Japan ,Epidemiology ,Influenza, Human ,biology.protein ,medicine ,Humans ,Seasons ,Antibody ,Child - Abstract
In Shizuoka Prefecture, influenza B viruses belonging to B/Victoria/2/87 lineage caused an outbreak among school children in the off-season of 1998, and the same B viruses were mainly isolated during the following epidemic. Low titer of HI antibody among children for influenza B virus belonging to the same lineage was a recognized factor in the causation of the distinctive epidemic. The herald virus strains seemed to be antigenically similar to the late epidemic virus strains, but the former strains were not genetically close to the latter. This indicates that the herald viruses are not always the parental viruses for the following influenza season.
- Published
- 2000
22. Incidence of Amantadine-resistant Influenza A Virus Isolated in 1999/2000 Epidemic Season in Shizuoka Prefecture
- Author
-
Hiromi Nagaoka, Hiromi Uenoyama, Masato Akiyama, Setsuko Nakajima, Keiji Sahara, and Masaaki Sugieda
- Subjects
Epidemic season ,Incidence (epidemiology) ,Amantadine ,Drug Resistance, Microbial ,General Medicine ,Biology ,medicine.disease_cause ,Antiviral Agents ,Virology ,Influenza A virus ,Child, Preschool ,medicine ,Humans ,Female ,medicine.drug - Published
- 2001
- Full Text
- View/download PDF
23. Outbreak of Influenza B Virus Infections in Summer Season
- Author
-
Hiromi Nagaoka, Hideki Miyamoto, Keiji Sahara, Masaaki Sugieda, and Yoshinobu Miwa
- Subjects
Veterinary medicine ,Adolescent ,Outbreak ,General Medicine ,Biology ,Virus ,Disease Outbreaks ,Summer season ,Influenza B virus ,Japan ,Influenza, Human ,Humans ,Seasons ,Child - Published
- 1999
- Full Text
- View/download PDF
24. Isolation of Coxiella burnetii from the Vagina of Feline Clients at Veterinary Clinics
- Author
-
Tokuhiro Nishina, Masaaki Sugieda, Masato Akiyama, Kôsaku Fujiwara, Hiromi Nagaoka, and Sousuke Akahane
- Subjects
General Veterinary ,Isolation (health care) ,biology ,business.industry ,Veterinary clinics ,Cat Diseases ,Coxiella burnetii ,biology.organism_classification ,Virology ,Mice ,medicine.anatomical_structure ,Vacuoles ,Vagina ,Cats ,Animals ,Medicine ,Female ,Q Fever ,business ,Cyclophosphamide ,Spleen - Published
- 1998
- Full Text
- View/download PDF
25. Identification of Rickettsiae Isolated in Japan as Coxiella burnetii by 16S rRNA Sequencing
- Author
-
Yasutake Yanagihara, Katsuya Hirai, Katsuji Sawaki, Masato Akiyama, Toshiyuki Masuzawa, and Hiromi Nagaoka
- Subjects
DNA, Bacterial ,Molecular Sequence Data ,Immunology ,Microbiology ,Rickettsiaceae ,Japan ,RNA, Ribosomal, 16S ,Genotype ,Animals ,Humans ,Gene ,DNA Primers ,biology ,Sequence Analysis, DNA ,Ribosomal RNA ,bacterial infections and mycoses ,biology.organism_classification ,Coxiella burnetii ,16S ribosomal RNA ,Virology ,Milk ,bacteria ,Cattle ,Q Fever ,Rickettsiales ,Bacteria - Abstract
The 16S rRNA genes of Japanese Coxiella isolates obtained from various sources and geographical areas were directly sequenced by dideoxynucleotide chain termination methods in which Taq DNA polymerase was used. The levels of sequence similarity among Japanese, European, and American isolates were more than 99%, and the Japanese isolates were identified as Coxiella burnetii, C. burnetii strains isolated worldwide, including Japan, were found to be very similar.
- Published
- 1997
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.