50 results on '"Hoang, Johnson"'
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2. The transcriptional response to low temperature is weakly conserved across the Enterobacteriaceae
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Hoang, Johnson, primary and Stoebel, Daniel, additional
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- 2024
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3. Transfection of Unmodified MicroRNA Using Monolayer-Coated Au Nanoparticles as Gene-Delivery Vehicles
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Hoang, Johnson, primary, Patil, Sagar L., additional, Srinoi, Pannaree, additional, Liu, Tingting, additional, Marquez, Maria D., additional, Khantamat, Orawan, additional, Tuntiwechapikul, Wirote, additional, Gunaratne, Preethi H., additional, and Lee, T. Randall, additional
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- 2023
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4. Factors Influencing Eye Screening Adherence Among Latinx Patients With Diabetes: A Qualitative Study
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Pelayo, Christian, primary, Mora Pinzón, Maria, additional, Lock, Loren J., additional, Fowlkes, Christiana, additional, Stevens, Chloe L., additional, Hoang, Johnson, additional, Garcia, Juan L., additional, Jacobson, Nora A., additional, Channa, Roomasa, additional, and Liu, Yao, additional
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- 2023
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- View/download PDF
5. Combined minimally invasive conjunctival surgery with lens repositioning for traumatic bleb leak with dislocated intraocular lens
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Hoang, Johnson, primary, Gutowski, Michal, additional, Altaweel, Michael, additional, and Liu, Yao, additional
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- 2023
- Full Text
- View/download PDF
6. Aqueous Dearomatization/Diels-Alder Cascade to a Grandifloracin Precursor
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Shimizu, Emily A., Cory, Brett, Hoang, Johnson, Castro, Giovanni G., Jung, Michael F., and Vosburg, David A.
- Abstract
A green laboratory experiment has been developed in which students perform an aqueous oxidation/cycloaddition reaction to convert salicyl alcohol into a pentacyclic diepoxydione that is readily isolated by filtration. Drawing on their knowledge of periodate-mediated 1,2-diol cleavage, students propose a mechanism for the oxidation of salicyl alcohol (which is not a 1,2-diol) and the structure of the transient product (prior to a spontaneous Diels-Alder dimerization). Students then characterize salicyl alcohol and their diepoxide product by mass spectrometry, IR spectroscopy, and [superscript 1]H, [superscript 13]C, and two-dimensional NMR spectroscopy. The only organic solvents used are small amounts for IR and NMR spectroscopy.
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- 2019
- Full Text
- View/download PDF
7. Perspectives of Latinx Patients with Diabetes on Teleophthalmology, Artificial Intelligence-Based Image Interpretation, and Virtual Care: A Qualitative Study
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Pelayo, Christian, primary, Hoang, Johnson, additional, Mora Pinzón, Maria, additional, Lock, Loren J., additional, Fowlkes, Christiana, additional, Stevens, Chloe L., additional, Jacobson, Nora A., additional, Channa, Roomasa, additional, and Liu, Yao, additional
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- 2023
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8. Self-Assembled Monolayers Derived from Positively Charged Adsorbates on Plasmonic Substrates for MicroRNA Delivery: A Review
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Hoang, Johnson, primary, Tajalli, Pooria, additional, Omidiyan, Mina, additional, Marquez, Maria D., additional, Khantamat, Orawan, additional, Tuntiwechapikul, Wirote, additional, Li, Chien-Hung, additional, Kohlhatkar, Arati, additional, Tran, Hung-Vu, additional, Gunaratne, Preethi H., additional, and Lee, T. Randall, additional
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- 2023
- Full Text
- View/download PDF
9. The timing of transcription of RpoS-dependent genes varies across multiple stresses in Escherichia coli K-12.
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Adams, Josephine, Hoang, Johnson, Petroni, Emily, Ashby, Ethan, Hardin, Johanna, and Stoebel, Daniel M.
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- 2023
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- View/download PDF
10. The timing of transcription of RpoS-dependent genes varies across multiple stresses
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Adams, Josephine, primary, Hoang, Johnson, additional, Petroni, Emily, additional, Ashby, Ethan, additional, Hardin, Johanna, additional, and Stoebel, Daniel M., additional
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- 2023
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11. Extreme mito-nuclear discordance within Anthozoa, with notes on unique properties of their mitochondrial genomes
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Quattrini, Andrea M., primary, Snyder, Karen, additional, Purow-Ruderman, Risa, additional, Seiblitz, Isabela G.L., additional, Hoang, Johnson, additional, Floerke, Natasha, additional, Ramos, Nina I., additional, Wirshing, Herman H., additional, Rodriguez, Estefanía, additional, and McFadden, Catherine S., additional
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- 2022
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12. Human Infection with Ehrlichia muris–like Pathogen, United States, 2007–2013
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Diep K. Hoang Johnson, Elizabeth Schiffman, Jeffrey P. Davis, David Neitzel, Lynne M. Sloan, William L. Nicholson, Thomas R. Fritsche, Christopher R. Steward, Julie A. Ray, Tracy K. Miller, Michelle A. Feist, Timothy S. Uphoff, Joni J. Franson, Amy L. Livermore, Alecia K. Deedon, Elitza S. Theel, and Bobbi Pritt
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Ehrlichia muris ,ehrlichiosis ,Ixodes scapularis ,bacteria ,United States ,epidemiology ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
An Ehrlichia muris–like (EML) pathogen was detected among 4 patients in Minnesota and Wisconsin during 2009. We characterized additional cases clinically and epidemiologically. During 2004–2013, blood samples from 75,077 patients from all 50 United States were tested by PCR from the groEL gene for Ehrlichia spp. and Anaplasma phagocytophilum. During 2007–2013, samples from 69 (0.1%) patients were positive for the EML pathogen; patients were from 5 states: Indiana (1), Michigan (1), Minnesota (33), North Dakota (3), and Wisconsin (31). Most (64%) patients were male; median age was 63 (range 15–94) years; and all 69 patients reported likely tick exposure in Minnesota or Wisconsin. Fever, malaise, thrombocytopenia, and lymphopenia were the most common symptoms. Sixteen (23%) patients were hospitalized (median 4 days); all recovered, and 96% received doxycycline. Infection with the EML pathogen should be considered for persons reporting tick exposure in Minnesota or Wisconsin.
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- 2015
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13. Passive Animal Surveillance to Identify Ticks in Wisconsin, 2011–2017
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Xia Lee, Darby S. Murphy, Diep Hoang Johnson, and Susan M. Paskewitz
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Ixodidae ,hard ticks ,host record ,county record ,Science - Abstract
The introduction of new tick species poses a risk to human and animal health. Systematic active surveillance programs are expensive and uncommon. We evaluated a passive animal surveillance program as a monitoring tool to document the geographic distribution and host associations of ticks in Wisconsin. Passive surveillance partners included veterinary medical clinics, domestic animal shelters, and wildlife rehabilitation centers from 35 of the 72 Wisconsin counties. A total of 10,136 tick specimens were collected from 2325 animals from July 2011 to November 2017 and included Dermacentor variabilis Say (29.7% of all ticks), Ixodes texanus Banks (25.5%), Ixodes scapularis Say (19.5%), Haemaphysalis leporispalustris Packard (13.8%), Ixodes cookei Packard (4.4%), and Dermacentor albipictus Packard (1.7%). Less common species (Ixodes dentatus Marx, Ixodes sculptus Neumann, Ixodes marxi Banks, Amblyomma americanum Linnaeus, and Rhipicephalus sanguineus Latreille. Of the 2325 animals that were examined, most were domestic dogs (53%), eastern cottontail rabbits (16%), domestic cats (15%), and North American raccoons (11%). An additional 21 mammal and 11 bird species were examined at least once during the six years of the study. New county records are summarized for each species. Public health, academic, and veterinary and animal care partners formed a community of practice enabling effective statewide tick surveillance.
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- 2019
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14. Barriers and facilitators to influenza-like illness absenteeism among healthcare workers in a tertiary-care healthcare system, 2017–2018 influenza season
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Julie A Keating, Diep Hoang Johnson, Shabvon Johnson, Jero Bean, Linda Stevens, Daniel Shirley, Fauzia Osman, and Nasia Safdar
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Microbiology (medical) ,medicine.medical_specialty ,Epidemiology ,Health Personnel ,Staffing ,03 medical and health sciences ,0302 clinical medicine ,Absenteeism ,Influenza, Human ,Health care ,Sore throat ,medicine ,Humans ,030212 general & internal medicine ,0303 health sciences ,Influenza-like illness ,030306 microbiology ,business.industry ,Odds ratio ,Cross-Sectional Studies ,Infectious Diseases ,Disciplinary action ,Family medicine ,Sick leave ,Seasons ,medicine.symptom ,business ,Delivery of Health Care - Abstract
Objective:Influenza can be introduced and propagated in healthcare settings by healthcare workers (HCWs) working while ill with influenza. However, reasons driving this behavior are unclear. In this study, we examined barriers to and facilitators of absenteeism during the influenza season.Design:Cross-sectional mixed methods study.Setting:Ambulatory and inpatient settings in a large, tertiary-care healthcare system.Methods:An anonymous electronic survey was sent to HCWs between June 11 and July 13, 2018, asking participants to self-report influenza-like illness (ie, ILI symptoms of fever, chills, cough, or sore throat) during the 2017–2018 influenza season. We conducted a logistical regression analysis to identify factors associated with absenteeism.Results:Of 14,250 HCWs, 17% responded to the survey. Although 1,180 respondents (51%) reported symptoms of ILI, 575 (43%) did not stay home while ill. The most commonly perceived barriers to ILI absenteeism included being understaffed (odds ratio [OR], 1.78; P = .04), unable to find a replacement for work (OR, 2.26; P = .03), desiring not to use time off (OR, 2.25; P = .003), and paid by the hour or unable to afford being absent (OR, 2.05; P = .02). Common perceived facilitators of absenteeism included support from coworkers and management, clearer policy, better sick days availability, and lower perceived threat of disciplinary action.Conclusions:Reporting to work with ILI symptoms is common among HCWs. Most barriers and facilitators are related to systems. Addressing system factors, such as policies regarding sick days and sick leave and ensuring adequate backup staffing, is likely to facilitate absenteeism among ill HCWs.
- Published
- 2021
15. Enhanced Arboviral Surveillance to Increase Detection of Jamestown Canyon Virus Infections, Wisconsin, 2011–2016
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Lina I Elbadawi, Maria C Mora-Pinzon, David M. Warshauer, Jeffrey P. Davis, J. Erin Staples, Mark V. Wegner, Diep K. Hoang Johnson, Eduard Matkovic, and Rebecca A. Osborn
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Adult ,Male ,Adolescent ,viruses ,030231 tropical medicine ,Population ,Encephalitis Virus, California ,Viral Plaque Assay ,Disease ,Antibodies, Viral ,Arbovirus ,Orthobunyavirus ,03 medical and health sciences ,Wisconsin ,0302 clinical medicine ,Encephalitis, California ,Meningoencephalitis ,Virology ,medicine ,Animals ,Humans ,Child ,education ,Aged ,Aged, 80 and over ,education.field_of_study ,Jamestown Canyon virus ,biology ,business.industry ,virus diseases ,Articles ,Middle Aged ,medicine.disease ,biology.organism_classification ,Meningitis, Viral ,Infectious Diseases ,Immunoglobulin M ,Epidemiological Monitoring ,Female ,Parasitology ,Public Health ,Seasons ,business ,Meningitis ,Encephalitis - Abstract
Jamestown Canyon virus (JCV), a mosquito-borne Orthobunyavirus (within the California serogroup), can cause severe neuroinvasive disease. According to national data during 2000–2013, 42% of the 31 documented JCV disease cases in the United States were detected in residents from Wisconsin. The Wisconsin Division of Public Health enhanced JCV surveillance by implementing routine use of JCV-specific immunoglobulin M (IgM) antibody testing followed by confirmatory JCV-specific plaque reduction neutralization testing on all patients with suspected cases of arboviral infection who had tests positive for arboviral immunoglobin at commercial laboratories. During 2011–2016, of the 287 Wisconsin specimens tested on the Arbovirus IgM Antibody Panel, 30 JCV cases were identified (26 confirmed and four probable). Twenty-seven (90%) JCV cases were detected after 2013. Among all cases, 17 (56%) were male and the median age was 54 years (range: 10–84 years). Fifteen patients had neuroinvasive disease, including meningitis (n = 9) and meningoencephalitis (n = 6). Although historically considered rare, the relatively high rate (0.12 cases/100,000 population) of diagnosis of JCV infections among Wisconsin residents during 2013–2016 compared with that in previous years suggests occurrence is widespread throughout Wisconsin and historically may have been under-recognized. This study aims to raise awareness of JCV infection for differential diagnosis among the arboviral diseases. Improved and timely diagnosis of arboviral disease is important in that it will provide more information regarding emerging infections and promote preventive measures to avoid mosquito-borne exposure and infection among residents of and visitors to affected areas.
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- 2019
16. Barriers and facilitators to influenza-like illness absenteeism among healthcare workers in a tertiary-care healthcare system, 2017–2018 influenza season
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Hoang Johnson, Diep, primary, Osman, Fauzia, additional, Bean, Jero, additional, Stevens, Linda, additional, Shirley, Daniel, additional, Keating, Julie A., additional, Johnson, Shabvon, additional, and Safdar, Nasia, additional
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- 2021
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17. Passive Animal Surveillance to Identify Ticks in Wisconsin, 2011–2017
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Susan M. Paskewitz, Diep Hoang Johnson, Darby S. Murphy, and Xia Lee
- Subjects
Ixodidae ,Rhipicephalus sanguineus ,030231 tropical medicine ,ved/biology.organism_classification_rank.species ,Zoology ,Tick ,Article ,Amblyomma americanum ,03 medical and health sciences ,0302 clinical medicine ,030212 general & internal medicine ,lcsh:Science ,Dermacentor variabilis ,Dermacentor albipictus ,biology ,Ixodes cookei ,ved/biology ,hard ticks ,host record ,biology.organism_classification ,3. Good health ,Ixodes scapularis ,Insect Science ,lcsh:Q ,county record ,Haemaphysalis leporispalustris - Abstract
The introduction of new tick species poses a risk to human and animal health. Systematic active surveillance programs are expensive and uncommon. We evaluated a passive animal surveillance program as a monitoring tool to document the geographic distribution and host associations of ticks in Wisconsin. Passive surveillance partners included veterinary medical clinics, domestic animal shelters, and wildlife rehabilitation centers from 35 of the 72 Wisconsin counties. A total of 10,136 tick specimens were collected from 2325 animals from July 2011 to November 2017 and included Dermacentor variabilis Say (29.7% of all ticks), Ixodes texanus Banks (25.5%), Ixodes scapularis Say (19.5%), Haemaphysalis leporispalustris Packard (13.8%), Ixodes cookei Packard (4.4%), and Dermacentor albipictus Packard (1.7%). Less common species (<, 1% of collection) included Ixodes dentatus Marx, Ixodes sculptus Neumann, Ixodes marxi Banks, Amblyomma americanum Linnaeus, and Rhipicephalus sanguineus Latreille. Of the 2325 animals that were examined, most were domestic dogs (53%), eastern cottontail rabbits (16%), domestic cats (15%), and North American raccoons (11%). An additional 21 mammal and 11 bird species were examined at least once during the six years of the study. New county records are summarized for each species. Public health, academic, and veterinary and animal care partners formed a community of practice enabling effective statewide tick surveillance.
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- 2019
- Full Text
- View/download PDF
18. RGS6 Mediates Effects of Voluntary Running on Adult Hippocampal Neurogenesis
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Gao, Yu, primary, Shen, Minjie, additional, Gonzalez, Jose Carlos, additional, Dong, Qiping, additional, Kannan, Sudharsan, additional, Hoang, Johnson T., additional, Eisinger, Brian E., additional, Pandey, Jyotsna, additional, Javadi, Sahar, additional, Chang, Qiang, additional, Wang, Daifeng, additional, Overstreet-Wadiche, Linda, additional, and Zhao, Xinyu, additional
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- 2020
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19. DNA Binding on Self-Assembled Monolayers Terminated with Mixtures of Ammonium and Trimethylammonium Groups: Toward a Gene-Delivery Platform
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Hoang, Johnson, primary, Park, Chul Soon, additional, Marquez, Maria D., additional, Gunaratne, Preethi H., additional, and Lee, T. Randall, additional
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- 2020
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20. Passive Animal Surveillance to Identify Ticks in Wisconsin, 2011–2017
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Lee, Xia, primary, Murphy, Darby S., additional, Hoang Johnson, Diep, additional, and Paskewitz, Susan M., additional
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- 2019
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21. Proposal to reclassify Ehrlichia muris as Ehrlichia muris subsp. muris subsp. nov. and description of Ehrlichia muris subsp. eauclairensis subsp. nov., a newly recognized tick-borne pathogen of humans
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Jeffrey P. Davis, Elizabeth Schiffman, David F. Neitzel, Tomoko Tajima, Lynne M. Sloan, Curtis M. Nelson, Christopher D. Paddock, Sandor E. Karpathy, Cynthia S. Goldsmith, Diep K. Hoang Johnson, Michelle E. J. Allerdice, Susan M. Paskewitz, Bobbi S. Pritt, Yasuko Rikihisa, and Ulrike G. Munderloh
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DNA, Bacterial ,0301 basic medicine ,Peromyscus ,Sequence analysis ,Minnesota ,030231 tropical medicine ,Ehrlichia ,Subspecies ,Microbiology ,Mice ,03 medical and health sciences ,Wisconsin ,Taxonomic Description ,0302 clinical medicine ,Japan ,parasitic diseases ,Animals ,Humans ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Ehrlichia muris ,Ixodes ,biology ,Ehrlichiosis ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Virology ,Anaplasmataceae ,Bacterial Typing Techniques ,030104 developmental biology ,Ixodes scapularis ,Female ,Rickettsiales - Abstract
We have previously described a novel taxon of the genus Ehrlichia (type strain WisconsinT), closely related to Ehrlichia muris, that causes human ehrlichiosis among patients with exposures to ticks in the upper midwestern USA. DNA from this bacterium was also detected in Ixodes scapularis and Peromyscus leucopus collected in Minnesota and Wisconsin. To determine the relationship between the E. muris-like agent (EMLA) and other species of the genus Ehrlichia phenotypic, genotypic and epidemiologic comparisons were undertaken, including sequence analysis of eight gene loci (3906 nucleotides) for 39 EMLA DNA samples and the type strain of E. muris AS145T. Three loci were also sequenced from DNA of nine strains of E. muris from mouse spleens from Japan. All sequences from E. muris were distinct from homologous EMLA sequences, but differences between them were less than those observed among other species of the genus Ehrlichia. Phenotypic comparison of EMLA and E. muris revealed similar culture and electron microscopic characteristics, but important differences were noted in their geographic distribution, ecological associations and behavior in mouse models of infection. Based on these comparisons, we propose that type strain WisconsinT represents a novel subspecies, Ehrlichia murissubsp. eauclairensis,subsp. nov. This strain is available through the Centers for Disease Control and Prevention Rickettsial Isolate Reference Collection (CRIRC EMU002T) and through the Collection de Souches de l’Unité des Rickettsies (CSURP2883 T). The subspecies Ehrlichia murissubsp. muris subsp. nov. is automatically created and the type strain AS145T is also available through the same collections (CRIRC EMU001T, CSUR E2T). Included is an emended description of E. muris.
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- 2017
22. Barriers and Facilitators to Injection Safety in Ambulatory Care Settings
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Diep Hoang Johnson, Nasia Safdar, Daniel Shirley, Claire Leback, Kelli Rogers, and Laura Anderson
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Microbiology (medical) ,Epidemiology ,Best practice ,Staffing ,030501 epidemiology ,Safety standards ,Injections ,Midwestern United States ,Interviews as Topic ,03 medical and health sciences ,Patient safety ,0302 clinical medicine ,Ambulatory care ,Ambulatory Care ,Infection control ,Medicine ,Humans ,030212 general & internal medicine ,Academic Medical Centers ,Cross Infection ,Infection Control ,business.industry ,medicine.disease ,Checklist ,Infectious Diseases ,Ambulatory ,Practice Guidelines as Topic ,Medical emergency ,Guideline Adherence ,Patient Safety ,0305 other medical science ,business - Abstract
OBJECTIVEIdentify factors referred to as barriers and facilitators that can prevent or assist safe injection practices in ambulatory care settings to guide quality improvement.DESIGNIn this mixed-methods study, we utilized observations and interviews.SETTINGThis study was conducted at ambulatory clinics at a midwestern academic medical center from May through August 2017. Sites included a variety of clinical settings that performed intramuscular, intradermal, intravenous, or intra-articular injections.PARTICIPANTS AND INTERVENTIONSDirect observations of injections and interviews of ambulatory care staff were conducted. An observation checklist was created, including standards of injection safety from nationally recognized guidelines. Interview questions were developed using the System Engineering Initiative for Patient Safety (SEIPS) model. Interviews were recorded, transcribed, and then coded by 2 investigators.RESULTSIn total, 106 observations and 36 interviews were completed at 21 clinics. Injection safety standards with the lowest adherence included using needleless access devices to prepare injections (33%) and the proper use of multidose vials (CONCLUSIONSPerceived barriers and facilitators to infection control elements of injection safety are interconnected with SEIPS elements of persons, organizations, technologies, tasks, and environment. Direct observations demonstrated that knowledge of safety injection standards does not necessarily translate to best practices and may not match self-reported data.Infect Control Hosp Epidemiol 2018;39:841–848
- Published
- 2018
23. Aqueous Dearomatization/Diels–Alder Cascade to a Grandifloracin Precursor
- Author
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Shimizu, Emily A., primary, Cory, Brett, additional, Hoang, Johnson, additional, Castro, Giovanni G., additional, Jung, Michael E., additional, and Vosburg, David A., additional
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- 2019
- Full Text
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24. Enhanced Arboviral Surveillance to Increase Detection of Jamestown Canyon Virus Infections, Wisconsin, 2011–2016
- Author
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Matkovic, Eduard, primary, Hoang Johnson, Diep K., additional, Staples, J. Erin, additional, Mora-Pinzon, Maria C., additional, Elbadawi, Lina I., additional, Osborn, Rebecca A., additional, Warshauer, David M., additional, Wegner, Mark V., additional, and Davis, Jeffrey P., additional
- Published
- 2019
- Full Text
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25. Quaternary Ammonium-Terminated Films Formed from Mixed Bidentate Adsorbates Provide a High-Capacity Platform for Oligonucleotide Delivery
- Author
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Hoang, Johnson, primary, Park, Chul Soon, additional, Lee, Han Ju, additional, Marquez, Maria D., additional, Zenasni, Oussama, additional, Gunaratne, Preethi H., additional, and Lee, T. Randall, additional
- Published
- 2018
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26. Loss of MeCP2 in immature neurons leads to impaired network integration
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Sun, Yi, primary, Gao, Yu, additional, Tidei, Joseph J, additional, Shen, Minjie, additional, Hoang, Johnson T, additional, Wagner, Daniel F, additional, and Zhao, Xinyu, additional
- Published
- 2018
- Full Text
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27. Prevalence and Distribution of Human and Tick Infections with the Ehrlichia muris-Like Agent and Anaplasma phagocytophilum in Wisconsin, 2009-2015
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Diep K. Hoang Johnson, Darby S. Murphy, Susan M. Paskewitz, Theoren Loo, Scott R. Larson, and Xia Lee
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0301 basic medicine ,Male ,Nymph ,Veterinary medicine ,Anaplasmosis ,Ixodidae ,animal diseases ,030231 tropical medicine ,030106 microbiology ,Ehrlichia ,Tick ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Wisconsin ,Virology ,parasitic diseases ,medicine ,Animals ,Humans ,Ehrlichia muris ,biology ,Ehrlichiosis ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Anaplasma phagocytophilum ,Tick Infestations ,Infectious Diseases ,Ixodes scapularis ,Ixodes ,Female - Abstract
Ehrlichiosis and anaplasmosis are important emerging tickborne zoonoses that affect both humans and animals. Knowledge of the geographic distribution and prevalence of Ehrlichia spp. and Anaplasma phagocytophilum in Wisconsin is important information as a baseline for future comparisons. Reported human cases between 2009 and 2015 were identified using the Wisconsin Electronic Disease Surveillance System (WEDSS) and mapped by county of residence. Vector surveillance was established using ticks collected from animals by partners, including veterinary medical clinics, domestic animal shelters, and wildlife rehabilitation centers from 40 Wisconsin counties. A total of 1835 Ixodes scapularis tick specimens (larvae, nymphs, and adults) were collected from 18 different domestic and wildlife species from July 2011 to November 2015. An additional 1136 nymphs were collected by drag sampling at 23 locations in 19 counties in 2015. A real-time PCR assay that detects and distinguishes several Ehrlichia species, including a pathogenic Ehrlichia muris-like agent (EMLA), and A. phagocytophilum was performed on adult and nymphal ticks. A total of 757 I. scapularis ticks (predominately adults) were tested from animal collections, with 67 (8.9%) individuals positive for A. phagocytophilum and 22 (2.9%) positive for EMLA DNA. Of the 1150 questing nymphs, 62 (5.4%) were positive for A. phagocytophilum and 10 (0.9%) were positive for EMLA DNA. Specimens of I. scapularis that were positive for A. phagocytophilum were found in 27 of the 33 counties surveyed. Specimens that were positive for EMLA were less common and were found in nine counties. This study provides the first statewide survey of I. scapularis ticks for these pathogens and indicates that the risk of human exposure is widely distributed.
- Published
- 2017
28. Prevalence ofBorrelia burgdorferiandAnaplasma phagocytophiluminIxodes scapularis(Acari: Ixodidae) Nymphs Collected in Managed Red Pine Forests in Wisconsin
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Kenneth F. Raffa, David R. Coyle, Diep K. Hoang Johnson, Michael A. McGeehin, Susan M. Paskewitz, Matthew Murphy, Xia Lee, and Robert J. Murphy
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Nymph ,Veterinary medicine ,Range (biology) ,Tick ,Polymerase Chain Reaction ,Wisconsin ,Animals ,Acari ,Borrelia burgdorferi ,Ecosystem ,Lyme Disease ,Ixodes ,General Veterinary ,biology ,Ecology ,Ehrlichiosis ,Pinus ,bacterial infections and mycoses ,biology.organism_classification ,Anaplasma phagocytophilum ,Infectious Diseases ,Ixodes scapularis ,Insect Science ,Parasitology ,Seasons ,Ixodidae - Abstract
Changes in the structure of managed red pine forests in Wisconsin caused by interacting root- and stem-colonizing insects are associated with increased abundance of the blacklegged tick, Ixodes scapularis Say, in comparison with nonimpacted stands. However, the frequency and variability of the occurrence of tick-borne pathogens in this coniferous forest type across Wisconsin is unknown. Red pine forests were surveyed from 2009 to 2013 to determine the prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum in questing I. scapularis nymphs. Polymerase chain reaction analysis revealed geographical differences in the nymphal infection prevalence (NIP) of these pathogens in red pine forests. In the Kettle Moraine State Forest (KMSF) in southeastern Wisconsin, NIP of B. burgdorferi across all years was 35% (range of 14.5-53.0%). At the Black River State Forest (BRSF) in western Wisconsin, NIP of B. burgdorferi across all years was 26% (range of 10.9-35.5%). Differences in NIP of B. burgdorferi between KMSF and BRSF were statistically significant for 2010 and 2011 and for all years combined (P < 0.05). NIP ofA. phagocytophilum (human agent) averaged 9% (range of 4.6-15.8%) at KMSF and 3% (range of 0-6.4%) at BRSF, and was significantly different between the sites for all years combined (P < 0.05). Differences in coinfection of B. burgdorferi and A. phagocytophilum were not statistically significant between KMSF and BRSF, with an average of 3.4% (range of 1.7-10.5%) and 2.5% (range of 0-5.5%), respectively. In 2013, the density of infected nymphs in KMSF and BRSF was 14 and 30 per 1000m2, respectively, among the highest ever recorded for the state. Differences in the density of nymphs and NIP among sites were neither correlated with environmental factors nor time since tick colonization. These results document significant unexplained variation in tick-borne pathogens between coniferous forests in Wisconsin that warrants further study.
- Published
- 2014
29. Barriers and Facilitators to Injection Safety in Ambulatory Care Settings
- Author
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Leback, Claire, primary, Hoang Johnson, Diep, additional, Anderson, Laura, additional, Rogers, Kelli, additional, Shirley, Daniel, additional, and Safdar, Nasia, additional
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- 2018
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30. Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States
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Bobbi S. Pritt, Lynne M. Sloan, Gongping Liu, Christopher R. Steward, David Boxrud, Xia Lee, Laurel B. Respicio-Kingry, Elizabeth Schiffman, David F. Neitzel, Jeffrey P. Davis, Diep K. Hoang Johnson, Robin Patel, Elitza S. Theel, Michelle A. Feist, Tracy K. Miller, Jeannine M. Petersen, Martin E. Schriefer, Susan M. Paskewitz, Adam J. Replogle, Luke C. Kingry, Cole L. Irish, Alecia Deedon, Jenna Bjork, and Paul S. Mead
- Subjects
0301 basic medicine ,DNA, Bacterial ,Minnesota ,030231 tropical medicine ,030106 microbiology ,Microbiology ,Article ,Serology ,Midwestern United States ,03 medical and health sciences ,0302 clinical medicine ,Lyme disease ,Wisconsin ,Borrelia mayonii ,Borrelia burgdorferi Group ,Borrelia ,RNA, Ribosomal, 16S ,parasitic diseases ,medicine ,Animals ,Humans ,Borrelia burgdorferi ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Lyme Disease ,biology ,Ixodes ,General Medicine ,Sequence Analysis, DNA ,biology.organism_classification ,16S ribosomal RNA ,medicine.disease ,bacterial infections and mycoses ,Virology ,Bacterial Typing Techniques ,Ixodes scapularis ,Genes, Bacterial ,Female - Abstract
Lyme borreliosis (LB) is a multisystem disease caused by spirochetes in the Borrelia burgdorferisensu lato (Bbsl) genospecies complex. We previously described a novel Bbsl genospecies (type strain MN14-1420T) that causes LB among patients with exposures to ticks in the upper midwestern USA. Patients infected with the novel Bbsl genospecies demonstrated higher levels of spirochetemia and somewhat differing clinical symptoms as compared with those infected with other Bbsl genospecies. The organism was detected from human specimens using PCR, microscopy, serology and culture. The taxonomic status was determined using an eight-housekeeping-gene (uvrA, rplB, recG, pyrG, pepX, clpX, clpA and nifS) multi-locus sequence analysis (MLSA) and comparison of 16S rRNA gene, flaB, rrf-rrl, ospC and oppA2 nucleotide sequences. Using a system threshold of 98.3 % similarity for delineation of Bbsl genospecies by MLSA, we demonstrated that the novel species is a member of the Bbsl genospecies complex, most closely related to B. burgdorferisensu stricto (94.7-94.9 % similarity). This same species was identified in Ixodes scapularis ticks collected in Minnesota and Wisconsin. This novel species, Borrelia mayonii sp. nov, is formally described here. The type strain, MN14-1420, is available through the Deutsche Sammlung von Mikroorganismen und Zelkulturen GmbH (DSM 102811) and the American Type Culture Collection (ATCC BAA-2743).
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- 2016
31. Human Infection with Ehrlichia muris-like Pathogen, United States, 2007-2013(1)
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Elizabeth Schiffman, Lynne M. Sloan, Amy L. Livermore, Diep K. Hoang Johnson, David F. Neitzel, Bobbi S. Pritt, Jeffrey P. Davis, Joni J. Franson, Alecia Deedon, Michelle A. Feist, Tracy K. Miller, Julie A. Ray, William L. Nicholson, Christopher R. Steward, Elitza S. Theel, Thomas R. Fritsche, and Timothy S. Uphoff
- Subjects
Microbiology (medical) ,Adult ,Male ,Ehrlichiosis ,Adolescent ,Minnesota ,vector-borne infections ,tickborne infections ,Serology ,Human Infection with Ehrlichia muris–like Pathogen, United States, 2007–2013 ,Ticks ,Wisconsin ,Zoonoses ,parasitic diseases ,medicine ,Ehrlichia muris ,Animals ,Humans ,Serologic Tests ,bacteria ,Pathogen ,Aged ,Aged, 80 and over ,biology ,Ehrlichia ,Research ,Middle Aged ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,Virology ,Anaplasma phagocytophilum ,Anaplasmataceae ,United States ,Infectious Diseases ,ehrlichiosis ,Ixodes scapularis ,surveillance ,Female ,epidemiology - Abstract
This pathogen has been detected in patients from 5 states, all of whom reported likely tick exposure in Minnesota or Wisconsin., An Ehrlichia muris–like (EML) pathogen was detected among 4 patients in Minnesota and Wisconsin during 2009. We characterized additional cases clinically and epidemiologically. During 2004–2013, blood samples from 75,077 patients from all 50 United States were tested by PCR from the groEL gene for Ehrlichia spp. and Anaplasma phagocytophilum. During 2007–2013, samples from 69 (0.1%) patients were positive for the EML pathogen; patients were from 5 states: Indiana (1), Michigan (1), Minnesota (33), North Dakota (3), and Wisconsin (31). Most (64%) patients were male; median age was 63 (range 15–94) years; and all 69 patients reported likely tick exposure in Minnesota or Wisconsin. Fever, malaise, thrombocytopenia, and lymphopenia were the most common symptoms. Sixteen (23%) patients were hospitalized (median 4 days); all recovered, and 96% received doxycycline. Infection with the EML pathogen should be considered for persons reporting tick exposure in Minnesota or Wisconsin.
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- 2015
32. Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high spirochaetaemia: a descriptive study
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Michelle A. Feist, Tracy K. Miller, Luke C. Kingry, Cole L. Irish, Bobbi S. Pritt, Julie A. Ray, Robin Patel, Susan M. Paskewitz, Lynne M. Sloan, Diep K. Hoang Johnson, Jeffrey P. Davis, Paul S. Mead, Elizabeth Schiffman, Martin E. Schriefer, Alecia Deedon, Laurel B. Respicio-Kingry, Jeannine M. Petersen, Christopher R. Steward, Jenna Bjork, Elitza S. Theel, Adam J. Replogle, Xia Lee, and David F. Neitzel
- Subjects
0301 basic medicine ,DNA, Bacterial ,Pathology ,medicine.medical_specialty ,030231 tropical medicine ,Spirochaetales Infections ,Polymerase Chain Reaction ,Article ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Lyme disease ,Borrelia burgdorferi Group ,Sensu ,Borrelia mayonii ,law ,parasitic diseases ,medicine ,Animals ,Humans ,Borrelia burgdorferi ,Polymerase chain reaction ,Lyme Disease ,biology ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Virology ,United States ,030104 developmental biology ,Infectious Diseases ,Ixodes scapularis ,Lyme disease microbiology ,Spirochaete - Abstract
Summary Background Lyme borreliosis is the most common tick-borne disease in the northern hemisphere. It is a multisystem disease caused by Borrelia burgdorferi sensu lato genospecies and characterised by tissue localisation and low spirochaetaemia. In this study we aimed to describe a novel Borrelia species causing Lyme borreliosis in the USA. Methods At the Mayo clinic, from 2003 to 2014, we tested routine clinical diagnostic specimens from patients in the USA with PCR targeting the oppA1 gene of B burgdorferi sensu lato. We identified positive specimens with an atypical PCR result (melting temperature outside of the expected range) by sequencing, microscopy, or culture. We collected Ixodes scapularis ticks from regions of suspected patient tick exposure and tested them by oppA1 PCR. Findings 100 545 specimens were submitted by physicians for routine PCR from Jan 1, 2003 to Sept 30, 2014. From these samples, six clinical specimens (five blood, one synovial fluid) yielded an atypical oppA1 PCR product, but no atypical results were detected before 2012. Five of the six patients with atypical PCR results had presented with fever, four had diffuse or focal rash, three had symptoms suggestive of neurological inclusion, and two were admitted to hospital. The sixth patient presented with knee pain and swelling. Motile spirochaetes were seen in blood samples from one patient and cultured from blood samples from two patients. Among the five blood specimens, the median oppA1 copy number was 180 times higher than that in 13 specimens that tested positive for B burgdorferi sensu stricto during the same time period. Multigene sequencing identified the spirochaete as a novel B burgdorferi sensu lato genospecies. This same genospecies was detected in ticks collected at a probable patient exposure site. Interpretation We describe a new pathogenic Borrelia burgdorferi sensu lato genospecies (candidatus Borrelia mayonii ) in the upper midwestern USA, which causes Lyme borreliosis with unusually high spirochaetaemia. Clinicians should be aware of this new B burgdorferi sensu lato genospecies, its distinct clinical features, and the usefulness of oppA1 PCR for diagnosis. Funding US Centers for Disease Control and Prevention Epidemiology and Laboratory Capacity for Infectious Diseases (ELC) Cooperative Agreement and Mayo Clinic Small Grant programme.
- Published
- 2015
33. Proposal to reclassify Ehrlichia muris as Ehrlichia muris subsp. muris subsp. nov. and description of Ehrlichia muris subsp. eauclairensis subsp. nov., a newly recognized tick-borne pathogen of humans
- Author
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Pritt, Bobbi S., primary, Allerdice, Michelle E. J., additional, Sloan, Lynne M., additional, Paddock, Christopher D., additional, Munderloh, Ulrike G., additional, Rikihisa, Yasuko, additional, Tajima, Tomoko, additional, Paskewitz, Susan M., additional, Neitzel, David F., additional, Hoang Johnson, Diep K., additional, Schiffman, Elizabeth, additional, Davis, Jeffrey P., additional, Goldsmith, Cynthia S., additional, Nelson, Curtis M., additional, and Karpathy, Sandor E., additional
- Published
- 2017
- Full Text
- View/download PDF
34. Jamestown Canyon Virus Disease in the United States—2000–2013
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Diep K. Hoang Johnson, Marc Fischer, Daniel M. Pastula, Alan P. Dupuis, Jennifer L. White, and J. Erin Staples
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Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Adolescent ,viruses ,Disease ,Encephalitis Virus, California ,Orthobunyavirus ,Young Adult ,Encephalitis, California ,Virology ,medicine ,Viral meningitis ,Animals ,Humans ,Child ,Aged ,Jamestown Canyon virus ,biology ,business.industry ,Public health ,Meningoencephalitis ,virus diseases ,Articles ,Middle Aged ,biology.organism_classification ,medicine.disease ,United States ,Infectious Diseases ,Culicidae ,Linear Models ,Parasitology ,Female ,business ,Meningitis ,Encephalitis - Abstract
Jamestown Canyon virus (JCV) is a mosquito-borne orthobunyavirus in the California serogroup that can cause an acute febrile illness, meningitis, or meningoencephalitis. We describe epidemiologic and clinical features for JCV disease cases occurring in the United States during 2000-2013. A case of JCV disease was defined as an acute illness in a person with laboratory evidence of a recent JCV infection. During 2000-2013, we identified 31 cases of JCV disease in residents of 13 states. The median age was 48 years (range, 10-69) and 21 (68%) were male. Eleven (35%) case patients had meningoencephalitis, 6 (19%) meningitis, 7 (23%) fever without neurologic involvement, and 7 (23%) had an unknown clinical syndrome. Fifteen (48%) were hospitalized and there were no deaths. Health-care providers and public health officials should consider JCV disease in the differential diagnoses of viral meningitis and encephalitis, obtain appropriate specimens for testing, and report cases to public health authorities.
- Published
- 2015
35. Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States
- Author
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Pritt, Bobbi S., primary, Respicio-Kingry, Laurel B., additional, Sloan, Lynne M., additional, Schriefer, Martin E., additional, Replogle, Adam J., additional, Bjork, Jenna, additional, Liu, Gongping, additional, Kingry, Luke C., additional, Mead, Paul S., additional, Neitzel, David F., additional, Schiffman, Elizabeth, additional, Hoang Johnson, Diep K., additional, Davis, Jeffrey P., additional, Paskewitz, Susan M., additional, Boxrud, David, additional, Deedon, Alecia, additional, Lee, Xia, additional, Miller, Tracy K., additional, Feist, Michelle A., additional, Steward, Christopher R., additional, Theel, Elitza S., additional, Patel, Robin, additional, Irish, Cole L., additional, and Petersen, Jeannine M., additional
- Published
- 2016
- Full Text
- View/download PDF
36. Loss of MeCP2 in immature neurons leads to impaired network integration.
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Sun, Yi, Gao, Yu, Tidei, Joseph J, Shen, Minjie, Hoang, Johnson T, Wagner, Daniel F, and Zhao, Xinyu
- Published
- 2019
- Full Text
- View/download PDF
37. Occurrence ofAmblyomma americanum(Acari: Ixodidae) and Human Infection WithEhrlichia chaffeensisin Wisconsin, 2008–2015
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Megan Christenson, Megan E. Meller, Susan M. Paskewitz, Diep K. Hoang Johnson, Scott R. Larson, Julia Jensen, and Xia Lee
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0106 biological sciences ,Ehrlichiosis ,Ixodidae ,Medical entomology ,030231 tropical medicine ,Zoology ,Tick ,01 natural sciences ,Amblyomma americanum ,03 medical and health sciences ,Wisconsin ,0302 clinical medicine ,parasitic diseases ,medicine ,Animals ,Humans ,Ehrlichia chaffeensis ,Acari ,General Veterinary ,biology ,Deer ,biology.organism_classification ,medicine.disease ,010602 entomology ,Infectious Diseases ,Insect Science ,Epidemiological Monitoring ,Arachnid Vectors ,Parasitology ,Tick dragging - Abstract
Because of the increasing incidence of human ehrlichiosis in Wisconsin, we assessed reports of human infections by Ehrlichia chaffeensis and the distribution of its vector, the lone star tick (Amblyomma americanum (L.)). From 2008 through 2015, 158 probable and confirmed human cases of E. chaffeensis infections were reported to the Wisconsin Department of Health Services. Five cases without travel history outside of Wisconsin were confirmed as E. chaffeensis by polymerase chain reaction. Surveillance for the vector occurred from 2008 through 2015 and was based on active and passive methods, including examination of white-tailed deer, collections from live-trapped small mammals, submissions of ticks removed from wild and domestic animals through the Wisconsin Surveillance of Animals for Ticks (SWAT) program, digital or physical submissions by the public to the University of Wisconsin Insect Diagnostic or Medical Entomology laboratories, and active tick dragging. More than 50 lone star ticks (46 adults, 6 nymphs, and 1 larva) were identified. Lone star ticks were more commonly found in south central Wisconsin, particularly in Dane County, where discovery of more than one life stage in a single year indicates possible establishment.
- Published
- 2016
38. Hunter-killed deer surveillance to assess changes in the prevalence and distribution of Ixodes scapularis (Acari: Ixodidae) in Wisconsin
- Author
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Susan M. Paskewitz, Xia Lee, Diep K. Hoang Johnson, and Kristin Hardy
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Male ,Veterinary medicine ,Range (biology) ,Distribution (economics) ,Odocoileus ,Lyme disease ,Ticks ,Wisconsin ,medicine ,Animals ,Acari ,General Veterinary ,biology ,Ixodes ,business.industry ,Incidence (epidemiology) ,Deer ,biology.organism_classification ,medicine.disease ,Insect Vectors ,Tick Infestations ,Infectious Diseases ,Ixodes scapularis ,Insect Science ,Parasitology ,Female ,Seasons ,business ,Animal Distribution ,Ixodidae - Abstract
As a result of the increasing incidence of Lyme disease and other tick-borne pathogens in Wisconsin, we assessed the distribution of adult blacklegged ticks through collections from hunter-killed deer in 2008 and 2009 and compared results with prior surveys beginning in 1981. Volunteers staffed 21 Wisconsin Department of Natural Resources registration stations in 21 counties in the eastern half of Wisconsin in 2008 and 10 stations in seven counties in northwestern Wisconsin in 2009. In total, 786 and 300 white-tailed deer (Odocoileus virginianus) were examined in 2008 and 2009, respectively. All but three stations in 2008 were positive for ticks and all stations in 2009 were positive for ticks. The three sites negative for ticks occurred within the eastern half of Wisconsin. The results indicate that range expansion of Ixodes scapularis (Say) is continuing and the risk of tick exposure is increasing, especially in the eastern one-third of the state.
- Published
- 2013
39. Emergence of a New Pathogenic Ehrlichia Species, Wisconsin and Minnesota, 2009
- Author
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Lynne M. Sloan, David M. Warshauer, Jennifer H. McQuiston, Mark P. Wilhelm, Vipul A. Trivedi, Diep K. Hoang Johnson, Bobbi S. Pritt, Ulrike G. Munderloh, Susan M. Paskewitz, William L. Nicholson, Scott A. Martin, Marina E. Eremeeva, Kristina M. McElroy, Christopher R. Steward, Robin Patel, Jevon McFadden, Mary E. Bjorgaard, Joni J. Franson, Gongping Liu, Matthew J. Binnicker, David F. Neitzel, Kay Bogumill, Curtis M. Nelson, Scott A. Cunningham, and Jeffrey P. Davis
- Subjects
Male ,Ehrlichiosis ,Minnesota ,Ehrlichia ,Polymerase Chain Reaction ,Article ,Serology ,Young Adult ,Wisconsin ,Zoonoses ,medicine ,Ehrlichia chaffeensis ,Animals ,Humans ,Phylogeny ,Ehrlichia muris ,biology ,Ixodes ,business.industry ,Zoonosis ,General Medicine ,Middle Aged ,biology.organism_classification ,medicine.disease ,Virology ,Ixodes scapularis ,Female ,business - Abstract
Ehrlichiosis is a clinically important, emerging zoonosis. Only Ehrlichia chaffeensis and E. ewingii have been thought to cause ehrlichiosis in humans in the United States. Patients with suspected ehrlichiosis routinely undergo testing to ensure proper diagnosis and to ascertain the cause.We used molecular methods, culturing, and serologic testing to diagnose and ascertain the cause of cases of ehrlichiosis.On testing, four cases of ehrlichiosis in Minnesota or Wisconsin were found not to be from E. chaffeensis or E. ewingii and instead to be caused by a newly discovered ehrlichia species. All patients had fever, malaise, headache, and lymphopenia; three had thrombocytopenia; and two had elevated liver-enzyme levels. All recovered after receiving doxycycline treatment. At least 17 of 697 Ixodes scapularis ticks collected in Minnesota or Wisconsin were positive for the same ehrlichia species on polymerase-chain-reaction testing. Genetic analyses revealed that this new ehrlichia species is closely related to E. muris.We report a new ehrlichia species in Minnesota and Wisconsin and provide supportive clinical, epidemiologic, culture, DNA-sequence, and vector data. Physicians need to be aware of this newly discovered close relative of E. muris to ensure appropriate testing, treatment, and regional surveillance. (Funded by the National Institutes of Health and the Centers for Disease Control and Prevention.).
- Published
- 2011
40. Jamestown Canyon Virus Disease in the United States—2000–2013
- Author
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Pastula, Daniel M., primary, Hoang Johnson, Diep K., additional, Fischer, Marc, additional, White, Jennifer L., additional, Staples, J. Erin, additional, and Dupuis, Alan P., additional
- Published
- 2015
- Full Text
- View/download PDF
41. Tickborne Powassan virus infections among Wisconsin residents
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Diep K Hoang, Johnson, J Erin, Staples, Mark J, Sotir, David M, Warshauer, and Jeffrey P, Davis
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Male ,Wisconsin ,Population Surveillance ,Humans ,Female ,Middle Aged ,Encephalitis, Tick-Borne ,Aged - Abstract
Powassan virus (POWV) is a tickborne Flavivirus that causes a rare but potentially life-threatening illness. The first reported case of POWV infection in a Wisconsin resident occurred in 2003. Enhanced surveillance and testing detected 2 additional cases.Patient specimens with a positive or equivocal immunoglobulin M (IgM) antibody to an arbovirus were sent from commercial laboratories to the Wisconsin State Laboratory of Hygiene and forwarded to the Centers for Disease Control and Prevention (CDC) for confirmatory testing. Patients with laboratory confirmed POWV infections were interviewed to obtain demographic, clinical, and epidemiologic information.POWV infections were confirmed in 3 adult Wisconsin residents in 2003, 2006, and 2007; illness onsets occurred during May and June. Two patients were hospitalized and all survived. One patient had a dual infection with POWV and Anaplasma phaghocytophilum. Specimens from all 3 patients were initially reported as positive for IgM antibody to either St Louis encephalitis or California serogroup viruses; POWV-specific antibody was detected during confirmatory testing at the CDC. Each patient had exposures to known or likely tick habitats in different counties within 30 days before illness onset.These are the first diagnosed human POWV infections in Wisconsin. Because all 3 patients were initially identified as having other arboviral infections using commercial screening kits, routine confirmatory testing is essential for proper diagnosis of most arboviral infections. Wisconsin residents should be educated regarding risks of acquiring and ways to prevent POWV infection and other tickborne diseases when spending time outdoors.
- Published
- 2010
42. Multistate outbreak of Escherichia coli O157:H7 infection associated with consumption of packaged spinach, August-September 2006: the Wisconsin investigation
- Author
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Diep K. Hoang Johnson, Cindy Koschmann, Jeffrey P. Davis, John R. Archer, Umid M. Sharapov, Juliana Grant, Arthur M. Wendel, and Timothy A. Monson
- Subjects
Microbiology (medical) ,Adult ,Male ,Veterinary medicine ,medicine.medical_specialty ,Adolescent ,media_common.quotation_subject ,Escherichia coli O157 ,Disease Outbreaks ,Foodborne Diseases ,Feces ,Wisconsin ,Hygiene ,Spinacia oleracea ,Internal medicine ,Epidemiology ,medicine ,Humans ,Child ,Escherichia coli Infections ,media_common ,Aged ,Aged, 80 and over ,business.industry ,Public health ,Case-control study ,Pulsenet ,Outbreak ,Infant ,Odds ratio ,Middle Aged ,Confidence interval ,Electrophoresis, Gel, Pulsed-Field ,Infectious Diseases ,Logistic Models ,Databases as Topic ,Case-Control Studies ,Child, Preschool ,Food Microbiology ,Female ,business - Abstract
Background. Escherichia coli O157:H7 infection often causes hemorrhagic colitis and hemolytic uremic syndrome. Methods. In 2006, the Wisconsin Division of Public Health and the Wisconsin State Laboratory of Hygiene, in cooperation with other local, state, and federal partners, investigated an outbreak of E. coli O157: H7 infection. Results. In September 2006, the Wisconsin Division of Public Health and the Wisconsin State Laboratory of Hygiene were able to link geographically dispersed E. coli O157: H7 isolates recovered from the stool samples of ill persons, all of which had the same pulsed-field gel electrophoresis pattern (i.e outbreak pattern). Investigators conducted a case-control study with control subjects (n = 86) matched to case patients (n = 49) by age, sex, and residential location. All case patients' onsets of illness occurred during the period from 20 August through 14 September 2006. Illness was associated with spinach consumption (matched odds ratio, 82.1; 95% confidence interval, 14.7 to >1000). Of the 49 case patients, 26 (53%) recalled eating brand A spinach. On multibrand analysis, only brand A was associated with illness (undefined matched odds ratio; 95% confidence interval, 6.8-infinity). Wisconsin's agriculture laboratory isolated E. coli O157:H7 with the outbreak pattern from spinach in 2 brand A packages, both produced on 15 August 2006. Conclusions. The rapid multijurisdictional epidemiologic and laboratory response, including timely pulsed-field gel electrophoresis pattern analysis and PulseNet posting, facilitated prompt voluntary recall of brand A spinach.
- Published
- 2009
43. Norovirus activity--United States, 2006-2007
- Author
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Jenkins, P., Greene, S., Davis, J.P., Archer, J.R., Hoang-Johnson, D., Quinn, M., Duncan, P., Johnson, G., Rosen, B.I., Smith, P., Reddy, V., Schlegelmilch, J., Pendarvis, J., Donovan, M., Gunn, J.E., Barry, M.A., Davies, M., Vinje, J., Widdowson, M-A., Moore, Z., Schaffzin, J.K., and Tate, J.E.
- Subjects
United States. Centers for Disease Control and Prevention -- Powers and duties ,United States. Centers for Disease Control and Prevention -- Reports ,Gastroenteritis -- Development and progression ,Gastroenteritis -- Statistics ,Gastroenteritis -- Prevention ,Norovirus -- Evaluation - Abstract
In late 2006, CDC began receiving requests from numerous state public health departments for information about a perceived increase in the number of outbreaks of acute gastroenteritis (AGE), especially those [...]
- Published
- 2007
44. Self-Assembled Monolayer Films Derived from Tridentate Cyclohexyl Adsorbates with Alkyl Tailgroups of Increasing Chain Length
- Author
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Singhana, Burapol, primary, Jamison, Andrew C., additional, Hoang, Johnson, additional, and Lee, T. Randall, additional
- Published
- 2013
- Full Text
- View/download PDF
45. Human Infection with Ehrlichia muris-like Pathogen, United States, 2007-2013(1).
- Author
-
Hoang Johnson, Diep K., Schiffman, Elizabeth K., Davis, Jeffrey P., Neitzel, David F., Sloan, Lynne M., Nicholson, William L., Fritsche, Thomas R., Steward, Christopher R., Ray, Julie A., Miller, Tracy K., Feist, Michelle A., Uphoff, Timothy S., Franson, Joni J., Livermore, Amy L., Deedon, Alecia K., Theel, Elitza S., Pritt, Bobbi S., and Johnson, Diep K Hoang
- Abstract
An Ehrlichia muris-like (EML) pathogen was detected among 4 patients in Minnesota and Wisconsin during 2009. We characterized additional cases clinically and epidemiologically. During 2004-2013, blood samples from 75,077 patients from all 50 United States were tested by PCR from the groEL gene for Ehrlichia spp. and Anaplasma phagocytophilum. During 2007-2013, samples from 69 (0.1%) patients were positive for the EML pathogen; patients were from 5 states: Indiana (1), Michigan (1), Minnesota (33), North Dakota (3), and Wisconsin (31). Most (64%) patients were male; median age was 63 (range 15-94) years; and all 69 patients reported likely tick exposure in Minnesota or Wisconsin. Fever, malaise, thrombocytopenia, and lymphopenia were the most common symptoms. Sixteen (23%) patients were hospitalized (median 4 days); all recovered, and 96% received doxycycline. Infection with the EML pathogen should be considered for persons reporting tick exposure in Minnesota or Wisconsin. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
46. Multistate Outbreak ofEscherichia coliO157:H7 Infection Associated with Consumption of Packaged Spinach, August–September 2006: The Wisconsin Investigation
- Author
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Wendel, Arthur M., primary, Hoang Johnson, Diep, additional, Sharapov, Umid, additional, Grant, Juliana, additional, Archer, John R., additional, Monson, Timothy, additional, Koschmann, Cindy, additional, and Davis, Jeffrey P., additional
- Published
- 2009
- Full Text
- View/download PDF
47. Multistate outbreak of Salmonella Typhimurium infections associated with eating ground beef--United States, 2004
- Author
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Cronquist, A., Wedel, S., Albanese, B., Sewell, C.M., Hoang-Johnson, D., Ihry, T., Lynch, M., Lockett, J., Kazerouni, N., O'Reilly, C., and Ferguson, D.
- Subjects
Beef -- Health aspects ,Infection -- Health aspects - Abstract
Salmonella infections cause an estimated 1.4 million human illnesses and 400 deaths annually in the United States (1). Although the incidence of several other foodborne bacterial infections decreased substantially during [...]
- Published
- 2006
48. Self-Assembled Monolayer Films Derived from TridentateCyclohexyl Adsorbates with Alkyl Tailgroups of Increasing Chain Length.
- Author
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Singhana, Burapol, Jamison, Andrew C., Hoang, Johnson, and Lee, T. Randall
- Published
- 2013
- Full Text
- View/download PDF
49. Transfection of Unmodified MicroRNA Using Monolayer-Coated Au Nanoparticles as Gene-Delivery Vehicles.
- Author
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Hoang J, Patil SL, Srinoi P, Liu T, Marquez MD, Khantamat O, Tuntiwechapikul W, Gunaratne PH, and Lee TR
- Subjects
- Humans, Gold chemistry, Transfection, Gene Transfer Techniques, MicroRNAs genetics, Metal Nanoparticles chemistry
- Abstract
This article describes a monolayer-coated gold nanoparticle-based transfection system for the delivery of microRNA (miRNA) into human osteosarcoma (HOS) cells. Two distinct ammonium-terminated adsorbates were used in this study, which provided a platform for ionic bonding of the miRNA onto gold nanoparticles (AuNPs). The custom-designed monolayer-coated gold nanoparticles were characterized by dynamic light scattering, gel mobility shift assay, transmission electron microscopy, ultraviolet-visible spectrometry, zeta potential, and X-ray photoelectron spectroscopy. The miRNA-loaded gold nanoparticles were transfected, and the level of intracellular miRNA delivered and taken up by cells was measured by Taqman qPCR. The overall analysis indicated a successful delivery of miRNA into the HOS cells at an ∼11,000-fold increase compared to nontreated cells.
- Published
- 2024
- Full Text
- View/download PDF
50. Travel-associated Dengue illnesses among Wisconsin residents, 2002-2008.
- Author
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Sotir MJ, Hoang Johnson DK, and Davis JP
- Subjects
- Adolescent, Adult, Aged, Child, Enzyme-Linked Immunosorbent Assay, Female, Humans, Interviews as Topic, Male, Middle Aged, Retrospective Studies, Risk Factors, Wisconsin epidemiology, Dengue epidemiology, Travel
- Abstract
Introduction: Dengue infections in humans can result in self-limited illness or conditions that can be severe and life-threatening. Persons traveling to many tropical regions are at risk for dengue infection. This report retrospectively summarizes travel-associated dengue cases occurring among Wisconsin residents from 2002 through 2008., Methods: We used a surveillance case definition based on the Centers for Disease Control and Prevention (CDC) 1996 dengue illness case definition. Detection of dengue-specific IgM antibody in serum specimens was used for laboratory confirmation of dengue. Clinical and travel histories, mosquito exposure, and repellent use were obtained from patients by interview using arbovirus-specific data collection forms., Results: During 2002-2008, 32 travel-associated dengue illnesses were reported among Wisconsin residents; none met the case criteria of dengue hemorrhagic fever or dengue shock syndrome. Fever (100%), headache (90%), and myalgia (87%) were the most frequently reported signs and symptoms. Nine (28%) patients were hospitalized; no deaths occurred. Onsets in 25 (81%) of 31 patients with reported travel histories occurred after return to Wisconsin. Eighteen (56%) of the 32 patients were female; median age was 35.5 years (range 12 to 68 years). Patients most frequently reported travel to Mexico/Central America (45%) or the Caribbean Islands (39%). Cases occurred during all months. Reported mosquito exposure was high among patients (85%), but consistent repellent use was low (6%)., Conclusions: Dengue illnesses occur in travelers to dengue-endemic tropical areas. Travelers to these areas must take precautions to prevent mosquito bites. Clinicians should consider dengue in travelers who develop febrile illnesses with headache or myalgia within 2 weeks of their return. Arboviral diseases, including dengue, are reportable in Wisconsin.
- Published
- 2009
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