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26 results on '"Hoben, Gwendolyn M."'

10. Vascularization is delayed in long nerve constructs compared to nerve grafts

Author

Farber, Scott J., Hoben, Gwendolyn M., Hunter, Daniel A., Yan, Ying, Johnson, Philip J., Mackinnon, Susan E., and Wood, Matthew D.

Subjects
Male, Platelet Endothelial Cell Adhesion Molecule-1, Disease Models, Animal, Isografts, Time Factors, Neovascularization, Pathologic, Rats, Inbred Lew, Animals, Transplantation, Homologous, Sciatic Neuropathy, Article, Nerve Regeneration, Rats
Abstract

Nerve regeneration across nerve constructs, such as acellular nerve allografts (ANAs), is inferior to nerve auto/isografts especially in the case of long defect lengths. Vascularization may contribute to poor regeneration. The time course of vascular perfusion within long grafts and constructs was tracked to determine vascularization.Male Lewis rat sciatic nerves were transected and repaired with 6 cm isografts or ANAs. At variable days following grafting, animals were perfused with Evans Blue albumin, and grafts were evaluated for vascular perfusion by a blinded observer.Vascularization at mid-graft was re-established within 3-4 days in 6 cm isografts, while it was established after 10 days in 6 cm ANAs.Vascular perfusion is reestablished over a shorter time course in long isografts when compared with long ANAs. The differences in vascularization of long ANAs compared with auto/isografts suggest regenerative outcomes across ANAs could be affected by vascularization rates. Muscle Nerve 54: 319-321, 2016.

Published
2016

11. Abstract 53

Author

Hoben, Gwendolyn M., primary, Ee, Xueping, additional, Schellhardt, Lauren, additional, Yan, Ying, additional, Hunter, Daniel, additional, Moore, Amy M., additional, Snyder-Warwick, Alison, additional, Stewart, Sheila, additional, Mackinnon, Susan E., additional, and Wood, Matthew D., additional

Published
2017
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14. Axonal Growth Arrests After an Increased Accumulation of Schwann Cells Expressing Senescence Markers and Stromal Cells in Acellular Nerve Allografts

Author

Poppler, Louis H., primary, Ee, Xueping, additional, Schellhardt, Lauren, additional, Hoben, Gwendolyn M., additional, Pan, Deng, additional, Hunter, Daniel A., additional, Yan, Ying, additional, Moore, Amy M., additional, Snyder-Warwick, Alison K., additional, Stewart, Sheila A., additional, Mackinnon, Susan E., additional, and Wood, Matthew D., additional

Published
2016
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16. Abstract 111

Author

Hoben, Gwendolyn M., primary, Ee, Xueping, additional, Prange, Lauren, additional, Hunter, Daniel, additional, Moore, Amy M., additional, Stewart, Sheila, additional, Mackinnon, Susan E., additional, and Wood, Matthew D., additional

Published
2015
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17. Abstract 54

Author

Hoben, Gwendolyn M., primary, Ee, Xueping, additional, Prange, Lauren, additional, Hunter, Daniel, additional, Moore, Amy M., additional, Stewart, Sheila, additional, Mackinnon, Susan E., additional, and Wood, Matthew D., additional

Published
2015
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19. Abstract 63

Author

Hoben, Gwendolyn M, primary, Newton, Piyaraj, additional, Hunter, Dan A, additional, Stewart, Sheila, additional, Johnson, Philip J, additional, Wood, Matthew D, additional, and Mackinnon, Susan E, additional

Published
2014
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25. Use of staurosporine, an actin-modifying agent, to enhance fibrochondrocyte matrix gene expression and synthesis.

Author

Hoben, Gwendolyn M. and Athanasiou, Kyriacos A.

Subjects
*GENE expression, *PROTEIN synthesis, *CARTILAGE cells, *CONNECTIVE tissues, *GENETIC regulation
Abstract

Modulation of the actin cytoskeleton in chondrocytes has been used to prevent or reverse dedifferentiation and to enhance protein synthesis. We have hypothesized that an actin-modifying agent, staurosporine, could be used with fibrochondrocytes to increase the gene expression and synthesis of critical fibrocartilage proteins. A range of concentrations (0.1–100 nM) was applied to fibrochondrocytes in monolayer and evaluated after 24 h and after 4 days. High-dose staurosporine treatment (10–100 nM) increased cartilage oligomeric matrix protein 60– to 500-fold and aggrecan gene expression two-fold. This effective range of staurosporine was then applied to scaffoldless tissue-engineered fibrochondrocyte constructs for 4 weeks. Whereas glycosaminoglycan synthesis was not affected, collagen content doubled, from 27.6 ± 8.8 μg in the untreated constructs to 55.2 ± 12.2 μg per construct with 100 nM treatment. When analyzed for specific collagens, the 10-nM group showed a significant increase in collagen type I content, whereas collagen type II was unaffected. A concomitant dose-dependent reduction was noted in construct contraction, reflecting the actin-disrupting action of staurosporine. Thus, staurosporine increases the gene expression for important matrix proteins and can be used to enhance matrix production and reduce contraction in tissue-engineered fibrocartilage constructs. [ABSTRACT FROM AUTHOR]

Published
2009
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