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Recent developments in workflows and techniques for the integration and analysis of terrestrial LiDAR (Light Detection And Ranging) and conventional outcrop datasets are demonstrated through three case studies. The first study shows the power of three-dimensional (3D) data visualization, in association with an innovative surface-modelling technique, for establishing large-scale 3D stratigraphical frameworks. The second presents an approach to derive reliable geometrical data on sediment-body geometries, whereas the third presents a new technique to quantify the proportions, distributions and variability of sedimentary facies directly from outcrop. In combination, these techniques provide essential conditioning data for geocellular and stochastic facies modelling. Built upon robust, reproducible and quantitative data, the resultant models combine realistic 3D geological architectures with sufficient quantities of reliable numerical data required for stable statistical analysis and establishing uncertainty. Together this new information provides detailed understanding and quantification of the 3D complexity of the sedimentary systems in question, thus offering insights of value for predicting the subsurface anatomy of analogous petroleum systems. As such, use of LiDAR, when combined with conventional field geology, offers a powerful tool for quantitative outcrop analysis, tightly constraining 3D structural and stratigraphical interpretations, and effectively increasing the statistical significance of outcrop analogues for reservoir characterization.

When imaginal disc fragments from Drosophila are cultured in adult female hosts, they either duplicate the part of the pattern specified by the fate map, or regenerate to replace the missing part. The new tissue is added by proliferation of a small number of cells from the cut edge, brought together when the wound heals to form a regeneration blastema. Specification of the new pattern has been explained by assuming interactions among cells of different positional value in the regeneration blastema. In order to identify genes which might mediate these events, we screened over eight hundred independently isolated autosomal insertions of an enhancer-sensitive P-element, for altered lac-z expression in regenerating discs following cell death induced by a temperature-sensitive cell-lethal mutation. Two further screens divided the positive lines into four groups based on appropriate timing of the lac-z response in the cell-lethal mutant background and the expected response to an alternate source of cell death. Expression in wing disc fragments cultured in vivo was most frequent in the target class defined by the screens. In this direct test, lac-z expression was found in 23 lines and in most cases was spatially and temporally correlated with the formation of the regeneration blastema. Our results suggest a very substantial transcriptional response during the early stages of imaginal disc regeneration. lac-z expression in control imaginal discs, embryos and adult ovaries of the positive lines was also assayed. The selected insertions included: a small class expressed only in discs undergoing regeneration and apparently not at any other stage, possibly representing genes active exclusively in regeneration; a larger class expressed in the embryo or during oogenesis, but not normally in imaginal discs, as expected for functions recruited from earlier stages of the developmental program; and finally a class with spatially patterned expression in normal discs. This class included several insertions with expression associated with compartment boundaries, including one at the decapentaplegic (dpp), and one at the crumbs (crb) locus, a growth factor homologue, and an EGF-repeat gene respectively. Some of the expression patterns observed in cultured disc fragments provide evidence for cell communication in the regeneration blastema.

A theoretical and experimental study of drive-line torsional vibrations of a European saloon car is reported. Vibration histories, taken from a number of stations along the drive-line, and passenger compartment noise levels, were recorded in a series of road tests. Resonances were identified from spectral maps and order plots based on the results of digital analyses. A torsional resonance was identified as causing high noise levels in the car.