28 results on '"Hoegl S"'
Search Results
2. The beta-adrenoceptor antagonist propranolol counteracts anti-inflammatory effects of isoflurane in rat endotoxemia
- Author
-
BOOST, K. A., FLONDOR, M., HOFSTETTER, C., PLATACIS, I., STEGEWERTH, K., HOEGL, S., NGUYEN, T., MUHL, H., and ZWISSLER, B.
- Published
- 2007
3. Wirkung von inhaliertem Iloprost auf systemische Inflammation und beatmungsinduzierten Lungenschaden
- Author
-
Flondor, M., primary, Hoegl, S., additional, Scheiermann, P., additional, Boost, K., additional, Zwissler, B., additional, and Hofstetter, C., additional
- Published
- 2009
- Full Text
- View/download PDF
4. Pulmonary ventilation and perfusion imaging with dual-energy CT.
- Author
-
Thieme SF, Hoegl S, Nikolaou K, Fisahn J, Irlbeck M, Maxien D, Reiser MF, Becker CR, Johnson TR, Thieme, Sven F, Hoegl, Sandra, Nikolaou, Konstantin, Fisahn, Juergen, Irlbeck, Michael, Maxien, Daniel, Reiser, Maximilian F, Becker, Christoph R, and Johnson, Thorsten R C
- Abstract
Objective: To evaluate the feasibility of dual-energy CT (DECT) ventilation imaging in combination with DE perfusion mapping for a comprehensive assessment of ventilation, perfusion, morphology and structure of the pulmonary parenchyma.Methods: Two dual-energy CT acquisitions for xenon-enhanced ventilation and iodine-enhanced perfusion mapping were performed in patients under artificial respiration. Parenchymal xenon and iodine distribution were mapped and correlated with structural or vascular abnormalities.Results: In all datasets, image quality was sufficient for a comprehensive image reading of the pulmonary CTA images, lung window images and pulmonary functional parameter maps and led to expedient results in each patient.Conclusion: With dual-source CT systems, DECT of the lung with iodine or xenon administration is technically feasible and makes it possible to depict the regional iodine or xenon distribution representing the local perfusion and ventilation. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
5. Inhaled levosimendan reduces mortality and release of proinflammatory mediators in a rat model of experimental ventilator-induced lung injury.
- Author
-
Boost KA, Hoegl S, Dolfen A, Czerwonka H, Scheiermann P, Zwissler B, and Hofstetter C
- Abstract
OBJECTIVES: Mechanical ventilation during critical care can cause structural and functional disturbances in the lung with subsequent release of proinflammatory mediators, termed ventilator-induced lung injury (VILI). VILI progressively provokes decreased efficiency of gas exchange with subsequent hypoxic pulmonary vasoconstriction leading to cardiopulmonary alterations, such as pulmonary hypertension and right heart failure. We therefore aimed to evaluate whether inhalation therapy with levosimendan, a calcium-sensitizer with pulmonary vasodilating properties, could attenuate VILI and improve short-term survival in a rat experimental model. DESIGN: Experimental animal model. SETTING: University hospital. SUBJECTS: Forty male Sprague-Dawley rats. INTERVENTIONS: Rats were randomly treated as follows (n = 8, each group): 1) inhalation of the solvent only before induction of VILI, no further intervention; 2) inhalation of 240 microg of levosimendan before VILI induction; 3) inhalation of 24 microg of levosimendan before VILI induction; 4) intravenous administration of 24 microg/kg levosimendan before VILI induction; 5) control group with surgical preparation only. All groups were observed for 4 hrs. MEASUREMENTS AND MAIN RESULTS: After 4 hrs following induction of VILI, levels of interleukin-1beta and macrophage inflammatory protein-2 in plasma and bronchoalveolar lavage fluid were analyzed by enzyme-linked immunosorbent assay. Nitric oxide release from alveolar macrophages was measured by Griess assay. Content of matrix metalloproteinase-2 and matrix metalloproteinase-9 in bronchoalveolar lavage fluid was analyzed by gelatin zymography. Inhalation of 240 microg of levosimendan significantly improved survival after 4 hrs and mean arterial blood pressure compared with VILI only. Additionally, inhalation of 240 microg and infusion of 24 microg/kg levosimendan significantly reduced the release of interleukin-1beta, the nitric oxide release from alveolar macrophages, macrophage inflammatory protein-2 in plasma, and the macrophage inflammatory protein-2 and matrix metalloproteinase-9 content in bronchoalveolar lavage fluid compared with VILI only. CONCLUSIONS: Our study demonstrates that prophylactic inhalation of 240 microg of levosimendan improves survival and reduces release of inflammatory mediators in our experimental model of VILI. This might affect the clinical prophylaxis and treatment of VILI. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
6. Cuff overinflation and endotracheal tube obstruction: case report and experimental study
- Author
-
Mack Martin G, Hoegl Sandra, Scheller Bertram, Hofstetter Christian, Zwissler Bernhard, and Byhahn Christian
- Subjects
Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Abstract Background Initiated by a clinical case of critical endotracheal tube (ETT) obstruction, we aimed to determine factors that potentially contribute to the development of endotracheal tube obstruction by its inflated cuff. Prehospital climate and storage conditions were simulated. Methods Five different disposable ETTs (6.0, 7.0, and 8.0 mm inner diameter) were exposed to ambient outside temperature for 13 months. In addition, every second of these tubes was mechanically stressed by clamping its cuffed end between the covers of a metal emergency case for 10 min. Then, all tubes were heated up to normal body temperature, placed within the cock of a syringe, followed by stepwise inflation of their cuffs to pressures of 3 kPa and ≥12 kPa, respectively. The inner lumen of the ETT was checked with the naked eye for any obstruction caused by the external cuff pressure. Results Neither in tubes that were exposed to ambient temperature (range: -12°C to +44°C) nor in those that were also clamped, visible obstruction by inflated cuffs was detected at any of the two cuff pressure levels. Conclusions We could not demonstrate a critical obstruction of an ETT by its inflated cuff, neither when the cuff was over-inflated to a pressure of 12 kPa or higher, nor in ETTs that had been exposed to unfavorable storage conditions and significant mechanical stress.
- Published
- 2010
- Full Text
- View/download PDF
7. Wirkung von inhaliertem Iloprost auf systemische Inflammation und beatmungsinduzierten Lungenschaden.
- Author
-
Flondor, M., Hoegl, S., Scheiermann, P., Boost, K. A., Zwissler, B., and Hofstetter, C.
- Published
- 2010
- Full Text
- View/download PDF
8. Digital Home-Monitoring of Patients after Kidney Transplantation: The MACCS Platform.
- Author
-
Duettmann W, Naik MG, Schmidt D, Pfefferkorn M, Kurz M, Graf V, Kreichgauer A, Hoegl S, Haenska M, Gielsdorf T, Breitenstein T, Osmanodja B, Glander P, Bakker J, Mayrdorfer M, Gethmann CJ, Bachmann F, Choi M, Schrezenmeier E, Zukunft B, Halleck F, and Budde K
- Subjects
- Humans, Kidney Diseases therapy, Electronic Health Records statistics & numerical data, Health Level Seven statistics & numerical data, Kidney Diseases physiopathology, Kidney Transplantation methods, Monitoring, Ambulatory methods, Software, Telemedicine
- Abstract
The MACCS (Medical Assistant for Chronic Care Service) platform enables secure sharing of key medical information between patients after kidney transplantation and physicians. Patients provide information such as vital signs, well-being, and medication intake via smartphone apps. The information is transferred directly into a database and electronic health record at the kidney transplant center, which is used for routine patient care and research. Physicians can send an updated medication plan and laboratory data directly to the patient app via this secure platform. Other features of the app are medical messages and video consultations. Consequently, the patient is better-informed, and self-management is facilitated. In addition, the transplant center and the patient's local nephrologist automatically exchange notes, medical reports, laboratory values, and medication data via the platform. A telemedicine team reviews all incoming data on a dashboard and takes action, if necessary. Tools to identify patients at risk for complications are under development. The platform exchanges data via a standardized secure interface (Health Level 7 (HL7), Fast Healthcare Interoperability Resources (FHIR)). The standardized data exchange based on HL7 FHIR guarantees interoperability with other eHealth solutions and allows rapid scalability to other chronic diseases. The underlying data protection concept is in concordance with the latest European General Data Protection Regulation. Enrollment started in February 2020, and 131 kidney transplant recipients are actively participating as of July 2020. Two large German health insurance companies are currently funding the telemedicine services of the project. The deployment for other chronic kidney diseases and solid organ transplant recipients is planned. In conclusion, the platform is designed to enable home monitoring and automatic data exchange, empower patients, reduce hospitalizations, and improve adherence, and outcomes after kidney transplantation.
- Published
- 2021
- Full Text
- View/download PDF
9. Capturing the multifactorial nature of ARDS - "Two-hit" approach to model murine acute lung injury.
- Author
-
Hoegl S, Burns N, Angulo M, Francis D, Osborne CM, Mills TW, Blackburn MR, Eltzschig HK, and Vohwinkel CU
- Subjects
- Animals, Female, Hydrochloric Acid toxicity, Lipopolysaccharides toxicity, Male, Mice, Mice, Inbred C57BL, Ventilator-Induced Lung Injury complications, Disease Models, Animal, Respiratory Distress Syndrome etiology, Respiratory Distress Syndrome physiopathology
- Abstract
Severe acute respiratory distress syndrome (ARDS) presents typically with an initializing event, followed by the need for mechanical ventilation. Most animal models of ALI are limited by the fact that they focus on a singular cause of acute lung injury (ALI) and therefore fail to mimic the complex, multifactorial pathobiology of ARDS. To better capture this scenario, we provide a comprehensive characterization of models of ALI combining two injuries: intra tracheal (i.t.) instillation of LPS or hypochloric acid (HCl) followed by ventilator-induced lung injury (VILI). We hypothesized, that mice pretreated with LPS or HCl prior to VILI and thus receiving a ("two-hit injury") will sustain a superadditive lung injury when compared to VILI. Mice were allocated to following treatment groups: control with i.t. NaCl, ventilation with low peak inspiratory pressure (PIP), i.t. HCl, i.t. LPS, VILI (high PIP), HCl i.t. followed by VILI and LPS i.t. followed by VILI. Severity of injury was determined by protein content and MPO activity in bronchoalveolar lavage (BAL), the expression of inflammatory cytokines and histopathology. Mice subjected to VILI after HCl or LPS instillation displayed augmented lung injury, compared to singular lung injury. However, mice that received i.t. LPS prior to VILI showed significantly increased inflammatory lung injury compared to animals that underwent i.t. HCl followed by VILI. The two-hit lung injury models described, resulting in additive but differential acute lung injury recaptures the clinical relevant multifactorial etiology of ALI and could be a valuable tool in translational research., (© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.)
- Published
- 2018
- Full Text
- View/download PDF
10. Preventing ventilator-induced lung injury-what does the evidence say?
- Author
-
Hoegl S and Zwissler B
- Abstract
Competing Interests: Conflicts of Interest: The authors have no conflicts of interest to declare.
- Published
- 2017
- Full Text
- View/download PDF
11. NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury.
- Author
-
Hoegl S, Ehrentraut H, Brodsky KS, Victorino F, Golden-Mason L, Eltzschig HK, and McNamee EN
- Subjects
- Acute Lung Injury complications, Adoptive Transfer, Animals, Antibodies metabolism, Cytokines metabolism, Inflammation Mediators metabolism, Lipopolysaccharides, Mice, Inbred C57BL, Pneumonia complications, Pneumonia immunology, Pneumonia pathology, T-Box Domain Proteins metabolism, Acute Lung Injury immunology, Acute Lung Injury pathology, Killer Cells, Natural immunology, Neutrophil Infiltration immunology, Receptors, Interleukin-8B metabolism
- Abstract
A critical step in the pathogenesis of acute lung injury (ALI) is excessive recruitment of polymorphonuclear neutrophils (PMNs) into the lungs, causing significant collateral tissue damage. Defining the molecular and cellular steps that control neutrophil infiltration and activation during ALI is therefore of important therapeutic relevance. Based on previous findings implicating the transcription factor Tbet in mucosal Th1-inflammation, we hypothesized a detrimental role for Tbet during ALI. In line with our hypothesis, initial studies of endotoxin-induced lung injury revealed a marked protection of Tbet
-/- mice, including attenuated neutrophilia compared to WT counterparts. Surprisingly, subsequent studies identified natural killer (NK) cells as the major source of pulmonary Tbet during ALI. In addition, a chemokine screen suggested that mature Tbet+ NK-cells are critical for the production of pulmonary CXCL1 and -2, thereby contributing to pulmonary PMN recruitment. Indeed, both NK-cell Ab depletion and adoptive transfer studies provide evidence for NK cells in the orchestration of neutrophil recruitment during endotoxin-induced ALI. Taken together, these findings identify a novel role for Tbet+ NK-cells in initiating the early events of noninfectious pulmonary inflammation., (© Society for Leukocyte Biology.)- Published
- 2017
- Full Text
- View/download PDF
12. Acute respiratory distress syndrome following cardiovascular surgery: current concepts and novel therapeutic approaches.
- Author
-
Hoegl S, Zwissler B, Eltzschig HK, and Vohwinkel C
- Subjects
- Humans, Respiration, Artificial adverse effects, Ventilator-Induced Lung Injury prevention & control, Ventilator-Induced Lung Injury therapy, Cardiovascular Surgical Procedures adverse effects, Postoperative Complications therapy, Respiratory Distress Syndrome etiology, Respiratory Distress Syndrome therapy
- Abstract
Purpose of Review: This review gives an update on current treatment options and novel concepts on the prevention and treatment of the acute respiratory distress syndrome (ARDS) in cardiovascular surgery patients., Recent Findings: The only proven beneficial therapeutic options in ARDS are those that help to prevent further ventilator-induced lung injury, such as prone position, use of lung-protective ventilation strategies, and extracorporeal membrane oxygenation. In the future also new approaches like mesenchymal cell therapy, activation of hypoxia-elicited transcription factors or targeting of purinergic signaling may be successful outside the experimental setting. Owing to the so far limited treatment options, it is of great importance to determine patients at risk for developing ARDS already perioperatively. In this context, serum biomarkers and lung injury prediction scores could be useful., Summary: Preventing ARDS as a severe complication in the cardiovascular surgery setting may help to reduce morbidity and mortality. As cardiovascular surgery patients are of greater risk to develop ARDS, preventive interventions should be implemented early on. Especially, use of low tidal volumes, avoiding of fluid overload and restrictive blood transfusion regimes may help to prevent ARDS.
- Published
- 2016
- Full Text
- View/download PDF
13. Hypoxia signaling during acute lung injury.
- Author
-
Vohwinkel CU, Hoegl S, and Eltzschig HK
- Subjects
- Animals, Humans, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Inflammation Mediators metabolism, Lung metabolism, Lung pathology, Acute Lung Injury metabolism, Hypoxia metabolism, Signal Transduction physiology
- Abstract
Acute lung injury (ALI) is an inflammatory lung disease that manifests itself in patients as acute respiratory distress syndrome and thereby contributes significantly to the morbidity and mortality of patients experiencing critical illness. Even though it may seem counterintuitive, as the lungs are typically well-oxygenated organs, hypoxia signaling pathways have recently been implicated in the resolution of ALI. For example, functional studies suggest that transcriptional responses under the control of the hypoxia-inducible factor (HIF) are critical in optimizing alveolar epithelial carbohydrate metabolism, and thereby dampen lung inflammation during ALI. In the present review we discuss functional roles of oxygenation, hypoxia and HIFs during ALI, mechanisms of how HIFs are stabilized during lung inflammation, and how HIFs can mediate lung protection during ALI., (Copyright © 2015 the American Physiological Society.)
- Published
- 2015
- Full Text
- View/download PDF
14. Alveolar Epithelial A2B Adenosine Receptors in Pulmonary Protection during Acute Lung Injury.
- Author
-
Hoegl S, Brodsky KS, Blackburn MR, Karmouty-Quintana H, Zwissler B, and Eltzschig HK
- Subjects
- Acute Lung Injury drug therapy, Acute Lung Injury genetics, Acute Lung Injury pathology, Adenosine A2 Receptor Agonists administration & dosage, Administration, Inhalation, Animals, Disease Models, Animal, Gene Deletion, Genetic Predisposition to Disease, Humans, Macrophages, Alveolar metabolism, Mice, Mice, Knockout, Neutrophils metabolism, Organ Specificity genetics, Receptor, Adenosine A2B genetics, Severity of Illness Index, Acute Lung Injury metabolism, Alveolar Epithelial Cells metabolism, Receptor, Adenosine A2B metabolism
- Abstract
Acute lung injury (ALI) is an acute inflammatory lung disease that causes morbidity and mortality in critically ill patients. However, there are many instances where ALI resolves spontaneously through endogenous pathways that help to control excessive lung inflammation. Previous studies have implicated the extracellular signaling molecule adenosine and signaling events through the A2B adenosine receptor in lung protection. In this context, we hypothesized that tissue-specific expression of the A2B adenosine receptor is responsible for the previously described attenuation of ALI. To address this hypothesis, we exposed mice with tissue-specific deletion of Adora2b to ALI, utilizing a two-hit model where intratracheal LPS treatment is followed by injurious mechanical ventilation. Interestingly, a head-to-head comparison of mice with deletion of Adora2b in the myeloid lineage (Adora2b(loxP/loxP) LysM Cre(+)), endothelial cells (Adora2b(loxP/loxP) VE-cadherin Cre(+)), or alveolar epithelial cells (Adora2b(loxP/loxP) SPC Cre(+)) revealed a selective increase in disease susceptibility in Adora2b(loxP/loxP) SPC Cre(+) mice. More detailed analysis of Adora2b(loxP/loxP) SPC Cre(+) mice confirmed elevated lung inflammation and attenuated alveolar fluid clearance. To directly deliver an A2B adenosine receptor-specific agonist to alveolar epithelial cells, we subsequently performed studies with inhaled BAY 60-6583. Indeed, aerosolized BAY 60-6583 treatment was associated with attenuated pulmonary edema, improved histologic lung injury, and dampened lung inflammation. Collectively, these findings suggest that alveolar epithelial A2B adenosine receptor signaling contributes to lung protection, and they implicate inhaled A2B adenosine receptor agonists in ALI treatment., (Copyright © 2015 by The American Association of Immunologists, Inc.)
- Published
- 2015
- Full Text
- View/download PDF
15. Worsening respiratory function in mechanically ventilated intensive care patients: feasibility and value of xenon-enhanced dual energy CT.
- Author
-
Hoegl S, Meinel FG, Thieme SF, Johnson TR, Eickelberg O, Zwissler B, and Nikolaou K
- Subjects
- Adult, Aged, Contrast Media, Female, Humans, Male, Middle Aged, Radiography, Dual-Energy Scanned Projection, Reproducibility of Results, Respiration Disorders etiology, Respiration, Artificial adverse effects, Sensitivity and Specificity, Respiration Disorders diagnosis, Respiration Disorders rehabilitation, Respiration, Artificial methods, Respiratory Function Tests methods, Tomography, X-Ray Computed methods, Xenon
- Abstract
Objectives: To evaluate the feasibility and incremental diagnostic value of xenon-enhanced dual-energy CT in mechanically ventilated intensive care patients with worsening respiratory function., Methods: The study was performed in 13 mechanically ventilated patients with severe pulmonary conditions (acute respiratory distress syndrome (ARDS), n=5; status post lung transplantation, n=5; other, n=3) and declining respiratory function. CT scans were performed using a dual-source CT scanner at an expiratory xenon concentration of 30%. Both ventilation images (Xe-DECT) and standard CT images were reconstructed from a single CT scan. Findings were recorded for Xe-DECT and standard CT images separately. Ventilation defects on xenon images were matched to morphological findings on standard CT images and incremental diagnostic information of xenon ventilation images was recorded if present., Results: Mean xenon consumption was 2.95 l per patient. No adverse events occurred under xenon inhalation. In the visual CT analysis, the Xe-DECT ventilation defects matched with pathologic changes in lung parenchyma seen in the standard CT images in all patients. Xe-DECT provided additional diagnostic findings in 4/13 patients. These included preserved ventilation despite early pneumonia (n=1), more confident discrimination between a large bulla and pneumothorax (n=1), detection of an airway-to-pneumothorax fistula (n=1) and exclusion of a suspected airway-to-mediastinum fistula (n=1). In all 4 patients, the additional findings had a substantial impact on patients' management., Conclusions: Xenon-enhanced DECT is safely feasible and can add relevant diagnostic information in mechanically ventilated intensive care patients with worsening respiratory function., (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
16. Effects of short-term infusion of lipid emulsions on pro-inflammatory cytokines and lymphocyte apoptosis in septic and non-septic rats.
- Author
-
Scheiermann P, Ott J, Hoegl S, Hecker M, Hofstetter C, Seeger W, Zwissler B, Bausch B, Boost KA, and Mayer K
- Subjects
- Animals, Cytokines genetics, Drug Administration Schedule, Fatty Acids, Nonesterified blood, Fish Oils administration & dosage, Fish Oils pharmacology, Gene Expression Regulation drug effects, Inflammation blood, Inflammation metabolism, Lymphocytes cytology, Male, Random Allocation, Rats, Rats, Sprague-Dawley, Soybean Oil administration & dosage, Soybean Oil pharmacology, Time Factors, Apoptosis drug effects, Cytokines metabolism, Fat Emulsions, Intravenous administration & dosage, Fat Emulsions, Intravenous pharmacology, Lymphocytes drug effects, Sepsis metabolism
- Abstract
Long-term administration of PUFA is known to modulate immune functions and apoptotic pathways depending on the respective amount of n-6 and n-3 fatty acids (FA). Data on short-term effects on apoptotic pathways are rare. Apoptosis of splenic lymphocytes is the hallmark of detrimental sepsis. Therefore, we aimed to compare the immediate effects of parenterally administered n-6-enriched soyabean oil (SO)- and n-3-enriched fish oil (FO)-based lipid emulsions after laparotomy (LAP; sham procedure) and after induction of acute, severe sepsis by caecal ligation and incision. After 390 min of observation time, plasma was analysed for IL-1β, IL-6 and NEFA. Apoptosis in splenic lymphocytes was quantified by Annexin-V expression. After LAP, infusion of both FO and SO did not change cytokine concentrations. Sepsis increased both cytokines. FO but not SO further augmented the rise. After LAP, SO increased NEFA, and both lipid emulsions reduced free arachidonic acid (AA). Sepsis resulted in a dramatic decrease in NEFA and AA. The drop in NEFA and AA was prevented by both SO and FO. In addition, FO resulted in an increased concentration of n-3 FA under both conditions. Infusion of both lipid emulsions induced apoptosis in splenic lymphocytes after LAP. Sepsis-induced apoptosis was not further enhanced by FO or SO. The present study shows that short-term administration of FO as opposed to SO caused pro-inflammatory effects during sepsis. Moreover, short-term administration of both SO and FO suffices to induce apoptosis in splenic lymphocytes. Finally, SO and FO do not further enhance sepsis-induced splenic apoptosis.
- Published
- 2011
- Full Text
- View/download PDF
17. Comparing hemodynamics, blood gas analyses and proinflammatory cytokines in endotoxemic and severely septic rats.
- Author
-
Scheiermann P, Hoegl S, Hofstetter C, Pfeilschifter J, Zwissler B, Mühl H, Boost KA, and Scheller B
- Subjects
- Acidosis, Animals, Blood Gas Analysis, Cecum immunology, Cecum surgery, Cells, Cultured, Cytokines genetics, Cytokines immunology, Endotoxemia chemically induced, Hemodynamics, Humans, Lipopolysaccharides administration & dosage, Male, Models, Animal, Rats, Rats, Sprague-Dawley, Cecum metabolism, Cytokines metabolism, Endotoxemia blood, Endotoxemia immunology, Inflammation Mediators metabolism
- Abstract
Lipopolysaccharide (LPS) is often used in short-term models of inflammation. Since endotoxemia and sepsis are different entities we have recently established a short-term sepsis model in rats induced by cecal ligation and incision (CLI). This retrospective study was conducted in order to identify similarities and differences between both experimental approaches. 32 anesthetized/ventilated male rats from the following four groups were analysed (each n=8): CTRL-group (0.9% NaCl i.v.); LPS-group (5mg/kg i.v.); SHAM-group (laparotomy); CLI-group (1.5 cm blade incision). Mean arterial blood pressure (MAP) and blood gas parameters (arterial base excess (BE) and pH) were continuously recorded. Total observation time was 300 min. Plasma samples were obtained afterwards. LPS and CLI induced significant arterial hypotension and metabolic acidosis compared to CTRL- or SHAM-group, respectively. Yet, between the LPS- and CLI-groups, there were no differences in MAP, BE and pH. LPS significantly induced IL-1β, IL-6 and TNF-α in the plasma. In contrast, CLI showed a clear tendency towards increased IL-1β and IL-6 plasma levels and did not affect TNF-α. Our results indicate that the CLI sepsis model is suitable for short-term investigations on hemodynamic alterations and blood gas analyses during sepsis. 300 min after the proinflammatory insult, plasma concentrations of IL-1β and IL-6 in the plasma remain considerably lower after CLI compared to endotoxemia. Low TNF-α concentrations 300 min after sepsis induction could be interpreted as considerable immunosuppression during CLI sepsis., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
18. Protective properties of inhaled IL-22 in a model of ventilator-induced lung injury.
- Author
-
Hoegl S, Bachmann M, Scheiermann P, Goren I, Hofstetter C, Pfeilschifter J, Zwissler B, and Muhl H
- Subjects
- Aerosols metabolism, Animals, Cell Line, Tumor, Disease Models, Animal, Epithelial Cells cytology, Humans, Inflammation, Lung metabolism, Lung Neoplasms metabolism, Male, Phosphorylation, Rats, Rats, Sprague-Dawley, STAT3 Transcription Factor metabolism, Interleukin-22, Interleukins metabolism, Ventilator-Induced Lung Injury metabolism
- Abstract
High-pressure ventilation induces barotrauma and pulmonary inflammation, thus leading to ventilator-induced lung injury (VILI). IL-22 has both immunoregulatory and tissue-protective properties. Functional IL-22 receptor expression is restricted to nonleukocytic cells, such as alveolar epithelial cells. When applied via inhalation, IL-22 reaches the pulmonary system directly and in high concentrations, and may protect alveolar epithelial cells against cellular stress and biotrauma associated with VILI. In A549 lung epithelial cells, IL-22 was able to induce rapid signal transducer and activator of transcription (STAT)-3 phosphorylation/activation, and hereon mediated stable suppressor of cytokine signaling (SOCS) 3 expression detectable even 24 hours after onset of stimulation. In a rat model of VILI, the prophylactic inhalation of IL-22 before induction of VILI (peak airway pressure = 45 cm H(2)O) protected the lung against pulmonary disintegration and edema. IL-22 reduced VILI-associated biotrauma (i.e., pulmonary concentrations of macrophage inflammatory protein-2, IL-6, and matrix metalloproteinase 9) and mediated pulmonary STAT3/SOCS3 activation. In addition, despite a short observation period of 4 hours, inhaled IL-22 resulted in an improved survival of the rats. These data support the hypothesis that IL-22, likely via activation of STAT3 and downstream genes (e.g., SOCS3), is able to protect against cell stretch and pulmonary baro-/biotrauma by enhancing epithelial cell resistibility.
- Published
- 2011
- Full Text
- View/download PDF
19. Cuff overinflation and endotracheal tube obstruction: case report and experimental study.
- Author
-
Hofstetter C, Scheller B, Hoegl S, Mack MG, Zwissler B, and Byhahn C
- Subjects
- Germany, Humans, Temperature, Air Pressure, Airway Obstruction etiology, Equipment Failure, Intubation, Intratracheal instrumentation
- Abstract
Background: Initiated by a clinical case of critical endotracheal tube (ETT) obstruction, we aimed to determine factors that potentially contribute to the development of endotracheal tube obstruction by its inflated cuff. Prehospital climate and storage conditions were simulated., Methods: Five different disposable ETTs (6.0, 7.0, and 8.0 mm inner diameter) were exposed to ambient outside temperature for 13 months. In addition, every second of these tubes was mechanically stressed by clamping its cuffed end between the covers of a metal emergency case for 10 min. Then, all tubes were heated up to normal body temperature, placed within the cock of a syringe, followed by stepwise inflation of their cuffs to pressures of 3 kPa and > or =12 kPa, respectively. The inner lumen of the ETT was checked with the naked eye for any obstruction caused by the external cuff pressure., Results: Neither in tubes that were exposed to ambient temperature (range: -12 degrees C to +44 degrees C) nor in those that were also clamped, visible obstruction by inflated cuffs was detected at any of the two cuff pressure levels., Conclusions: We could not demonstrate a critical obstruction of an ETT by its inflated cuff, neither when the cuff was over-inflated to a pressure of 12 kPa or higher, nor in ETTs that had been exposed to unfavorable storage conditions and significant mechanical stress.
- Published
- 2010
- Full Text
- View/download PDF
20. Effects of intravenous and inhaled levosimendan in severe rodent sepsis.
- Author
-
Scheiermann P, Ahluwalia D, Hoegl S, Dolfen A, Revermann M, Zwissler B, Muhl H, Boost KA, and Hofstetter C
- Subjects
- Administration, Inhalation, Animals, Humans, Hydrazones administration & dosage, Models, Animal, Phosphodiesterase Inhibitors administration & dosage, Pyridazines administration & dosage, Random Allocation, Rats, Rats, Sprague-Dawley, Rodentia, Sepsis diagnosis, Sepsis etiology, Severity of Illness Index, Simendan, Survival Analysis, Treatment Outcome, Hydrazones pharmacology, Injections, Intraventricular, Phosphodiesterase Inhibitors pharmacology, Pyridazines pharmacology, Sepsis drug therapy
- Abstract
Purpose: We aimed at comparing the effects of intravenous (i.v.) and inhaled (inh.) levosimendan (LEVO) on survival, inflammatory cytokines and the apoptotic mediator caspase-3 in a rat model of severe sepsis induced by cecal ligation and incision (CLI)., Methods: Twenty-eight anesthetized/ventilated male Sprague-Dawley rats (body weight 528 +/- 20 g) underwent laparotomy. Cecal mobilisation served as control (SHAM, n = 7). In all other groups, severe sepsis was induced by CLI. No further intervention occurred in the CLI-group (n = 7). 180 min after CLI, 24 microg/kg i.v. LEVO was administered in the CLI + LEVO-IV-group (n = 7), and 24 microg/kg inh. LEVO was administered via jet nebulizer in the CLI + LEVO-INH-group (n = 7)., Results: CLI induced arterial hypotension, with i.v. and inh. LEVO attenuating blood pressure decrease over 390 min [CLI 34(31/50), CLI + LEVO-IV 82(69/131)*, CLI + LEVO-INH 78(62/85)* mmHg; median(25/75% quartile), *P < 0.05]. CLI induced metabolic acidosis. I.v. and inh. LEVO avoided arterial pH [CLI 7.18(7.16/7.2), CLI + LEVO-IV 7.27(7.24/7.31)*, CLI + LEVO-INH 7.26(7.24/7.28)*] and base excess deterioration [CLI -19(-21.8/-17.9), CLI + LEVO-IV -13(-14.8/-12)*, CLI + LEVO-INH -12.7(-14/-12.2)* mmol/l]. Overall mortality in the CLI-group was 57% compared to 0%* in both LEVO-treated groups after 390 min. LEVO administration significantly attenuated the increase in proinflammatory interleukin (IL)-1beta [CLI 896(739/911), CLI + LEVO-IV 302(230/385)*, CLI + LEVO-INH 346(271/548) pg/ml] and IL-6 [CLI 35651(31413/35816), CLI + LEVO-IV 21156(18397/28026), CLI + LEVO-INH 13674(10105/24843) pg/ml] in the plasma and reduced cleaved caspase-3 expression in the spleen., Conclusions: In a rat model of severe sepsis induced by CLI, i.v. and inh. LEVO equally attenuated arterial hypotension, metabolic acidosis and prolonged survival. Moreover, i.v. and inh. LEVO inhibited proinflammatory mediator release and reduced splenic caspase-3 expression.
- Published
- 2009
- Full Text
- View/download PDF
21. Sevoflurane and isoflurane decrease TNF-alpha-induced gene expression in human monocytic THP-1 cells: potential role of intracellular IkappaBalpha regulation.
- Author
-
Boost KA, Leipold T, Scheiermann P, Hoegl S, Sadik CD, Hofstetter C, and Zwissler B
- Subjects
- Anesthetics, Inhalation pharmacology, Cell Line, Down-Regulation drug effects, Gene Expression Regulation drug effects, Heme Oxygenase-1 genetics, Heme Oxygenase-1 metabolism, Humans, I-kappa B Proteins metabolism, Interleukin-8 genetics, Interleukin-8 metabolism, Intracellular Fluid metabolism, NF-KappaB Inhibitor alpha, NF-kappa B metabolism, Protein Transport drug effects, Sevoflurane, I-kappa B Proteins physiology, Isoflurane pharmacology, Methyl Ethers pharmacology, Monocytes drug effects, Tumor Necrosis Factor-alpha pharmacology
- Abstract
The nuclear factor (NF)-kappaB/inhibitory (I)kappaBalpha pathway is one of the most important intracellular signal transduction pathways during inflammation which is induced by a variety of major early response cytokines. Recent studies suggest that volatile anesthetics interfere with inflammatory cytokine production through inhibition of intracellular signal transduction pathways. We, therefore, aimed to investigate the effects of the volatile anesthetics sevoflurane and isoflurane on NF-kappaB/IkappaBalpha-dependent intracellular signal transduction in human monocytic THP-1 cells induced by tumor necrosis factor-alpha (TNF-alpha) and production of interleukin-8 (IL-8) and downstream heme oxygenase-1 (HO-1). THP-1 cells, a human monocytic cell line, were used in an in vitro model which enables the exposure to volatile anesthetics. Using this model, THP-1 cells were subjected to sevoflurane or isoflurane exposure (1 MAC each) and were stimulated with TNF-alpha (50 or 100 ng/ml). Compared to untreated cells, expression of intracellular HO-1-protein and release of IL-8 into cell culture supernatants and corresponding mRNA expression were attenuated in THP-1 cells exposed to sevoflurane and isoflurane, respectively. Moreover, translocation of NF-kappaB and degradation of IkappaBalpha were markedly reduced by both anesthetics. Notably, under unstimulated conditions, exposure to sevoflurane induced a sustained upregulation of the IkappaBalpha content in THP-1 cells. We demonstrated inhibition of TNF-alpha-induced gene expression and release of IL-8 and HO-1 in human monocytic THP-1 cells exposed to both volatile anesthetics. This was associated with an upregulated intracellular IkappaBalpha content followed by decreased NF-kappaB translocation. This was more sustained during exposure to sevoflurane and may provide an additional intracellular mechanism for the anti-inflammatory effects associated with sevoflurane administration.
- Published
- 2009
- Full Text
- View/download PDF
22. Inhaled IL-10 reduces biotrauma and mortality in a model of ventilator-induced lung injury.
- Author
-
Hoegl S, Boost KA, Czerwonka H, Dolfen A, Scheiermann P, Muhl H, Zwissler B, and Hofstetter C
- Subjects
- Administration, Inhalation, Animals, Biomarkers analysis, Biomarkers blood, Bronchoalveolar Lavage Fluid chemistry, Chemokine CXCL2 analysis, Chemokine CXCL2 blood, Disease Models, Animal, HSP70 Heat-Shock Proteins analysis, Interleukin-10 therapeutic use, Interleukin-1beta analysis, Interleukin-1beta blood, Lung metabolism, Lung pathology, Lung physiopathology, Macrophages, Alveolar metabolism, Male, Matrix Metalloproteinase 9 analysis, Positive-Pressure Respiration adverse effects, Random Allocation, Rats, Rats, Sprague-Dawley, Ventilator-Induced Lung Injury metabolism, Ventilator-Induced Lung Injury mortality, Interleukin-10 administration & dosage, Ventilator-Induced Lung Injury prevention & control
- Abstract
Background: High-pressure ventilation induces barotrauma and pulmonary inflammation, thus leading to ventilator-induced lung injury (VILI). By limiting the pulmonal inflammation cascade the anti-inflammatory cytokine interleukin (IL)-10 may have protective effects. Via inhalation, IL-10 reaches the pulmonary system directly and in high concentrations., Methods: Thirty six male, anesthetized and mechanically ventilated Sprague-Dawley rats were randomly assigned to the following groups (n=9, each): SHAM: pressure controlled ventilation with p(max)=20cmH(2)O, PEEP=4; VILI: ventilator settings were changed for 20min to p(max)=45cmH(2)O, PEEP=0; IL-10(high): inhalation of 10microg/kg IL-10 prior to induction of VILI; and IL-10(low): inhalation of 1microg/kg IL-10 prior to induction of VILI. All groups were ventilated and observed for 4h., Results: High-pressure ventilation increased the concentrations of macrophage inflammatory protein (MIP)-2 and IL-1beta in bronchoalveolar lavage fluid (BALF) and plasma. This effect was reduced by the inhalation of IL-10 (10microg/kg). Additionally, IL-10 increased the animal survival time (78% vs. 22% 4-h mortality rate) and reduced NO-release from ex vivo cultured alveolar macrophages. Moreover, VILI-induced pulmonary heat shock protein-70 expression was reduced by IL-10 aerosol in a dose-dependent manner. Similarly, the activation of matrix metalloproteinase (MMP)-9 in BALF was reduced dose-dependently by IL-10. IL-10-treated animals showed a lower macroscopic lung injury score and less impairment of lung integrity and gas exchange., Conclusions: Prophylactic inhalation of IL-10 improved survival and reduced lung injury in experimental VILI. Results indicate that this effect may be mediated by the inhibition of stress-induced inflammation and pulmonary biotrauma.
- Published
- 2009
- Full Text
- View/download PDF
23. Cecal ligation and incision: an acute onset model of severe sepsis in rats.
- Author
-
Scheiermann P, Hoegl S, Revermann M, Ahluwalia D, Zander J, Boost KA, Nguyen T, Zwissler B, Muhl H, and Hofstetter C
- Subjects
- Acidosis physiopathology, Acute Disease, Animals, Blood Pressure physiology, Body Temperature physiology, Heart Rate physiology, Hydrogen-Ion Concentration, Interleukin-1beta blood, Interleukin-6 blood, Ligation, Male, Peritoneum microbiology, Peritonitis physiopathology, Rats, Rats, Sprague-Dawley, Sepsis physiopathology, Tidal Volume physiology, Cecum surgery, Disease Models, Animal, Peritonitis microbiology, Sepsis microbiology
- Abstract
Background: Sepsis is a leading cause of death among critically ill patients. Up to now, severe sepsis with acute onset in animals has been induced mainly through injection of single bacteria species or endotoxin and not through a surgical procedure, which might adequately mirror the situation in septic patients. We therefore aimed to establish a surgical model of severe sepsis in rodents fulfilling international sepsis criteria., Materials and Methods: Twenty-eight anesthetized/ventilated Sprague Dawley rats underwent laparotomy and cecal mobilization. The cecum was either replaced into the abdomen (SHAM, n = 14) or the cecum and the mesenteric blood vessels were ligated, and the cecum was opened through a 1.5 cm blade incision (cecal ligation and incision, CLI, n = 14)., Results: Within 390 min, mortality was 0% (SHAM) and 50% (CLI), respectively. Compared with SHAM, CLI resulted in a 43% reduction of mean arterial blood pressure and in severe metabolic acidosis as measured by arterial base excess and pH. CLI led to a 15-fold increase in mononuclear cell population and to a 5-fold accumulation of nitrite in peritoneal lavage. Abdominal swabs from the Douglas cavity in CLI-animals showed gram-positive and gram-negative bacterial growth on agar compared with sterile swabs from SHAM-animals. In CLI-animals, plasma IL-1beta level was increased to 435 pg/mL (SHAM: 10 pg/mL) and plasma IL-6 level to 19718 pg/mL (SHAM: 832 pg/mL)., Conclusions: CLI causes bacterial peritonitis with subsequent systemic inflammation and organ dysfunction. Thus, CLI mimics clinical sepsis and provides a surgical short term model of severe sepsis in rodents.
- Published
- 2009
- Full Text
- View/download PDF
24. Short-term exposure to high-pressure ventilation leads to pulmonary biotrauma and systemic inflammation in the rat.
- Author
-
Hoegl S, Boost KA, Flondor M, Scheiermann P, Muhl H, Pfeilschifter J, Zwissler B, and Hofstetter C
- Subjects
- Animals, Bronchoalveolar Lavage Fluid cytology, Bronchoalveolar Lavage Fluid immunology, Chemokine CXCL2 metabolism, Inflammation pathology, Inflammation physiopathology, Interleukin-1beta metabolism, Lung pathology, Lung physiopathology, Male, Multiple Organ Failure etiology, Multiple Organ Failure pathology, Multiple Organ Failure physiopathology, Rats, Rats, Sprague-Dawley, Time Factors, Inflammation etiology, Lung Injury, Positive-Pressure Respiration adverse effects
- Abstract
Though often lifesaving, mechanical ventilation itself bears the risk of lung damage [ventilator-induced lung injury (VILI)]. The underlying molecular mechanisms have not been fully elucidated, but stress-induced mediators seem to play an important role in biotrauma related to VILI. Our purpose was to evaluate an animal model of VILI that allows the observation of pathophysiologic changes along with parameters of biotrauma. For VILI induction, rats (n=16) were ventilated with a peak airway pressure (pmax) of 45 cm H2O and end-expiratory pressure (PEEP) of 0 for 20 min, followed by an observation time of 4 h. In the control group (n=8) the animals were ventilated with a pmax of 20 cm H2O and PEEP of 4. High-pressure ventilation resulted in an increase in paCO2 and a decrease in paO2 and mean arterial pressure. Only 4 animals out of 16 survived 4 h and VILI lungs showed severe macroscopic and microscopic damage, oedema and neutrophil influx. High-pressure ventilation increased the cytokine levels of macrophage inflammatory protein-2 and IL-1beta in bronchoalveolar lavage and plasma. VILI also induced pulmonary heat shock protein-70 expression and the activity of matrix metalloproteinases. The animal model used enabled us to observe the effect of high-pressure ventilation on mortality, lung damage/function and biotrauma. Thus, by combining barotrauma with biotrauma, this animal model may be suitable for studying therapeutical approaches to VILI.
- Published
- 2008
25. Norepinephrine and vasopressin counteract anti-inflammatory effects of isoflurane in endotoxemic rats.
- Author
-
Hofstetter C, Boost KA, Hoegl S, Flondor M, Scheller B, Muhl H, Pfeilschifter J, and Zwissler B
- Subjects
- Animals, Blood Pressure drug effects, Cells, Cultured, Cytokines blood, Drug Interactions, Endotoxemia chemically induced, Heart Rate drug effects, Humans, Lipopolysaccharides pharmacology, Macrophages, Alveolar drug effects, Macrophages, Alveolar metabolism, Male, Nitrites metabolism, Oxygen metabolism, Rats, Rats, Sprague-Dawley, Subcellular Fractions drug effects, Anti-Inflammatory Agents pharmacology, Endotoxemia pathology, Isoflurane pharmacology, Norepinephrine pharmacology, Vasopressins pharmacology
- Abstract
Volatile anesthetics such as isoflurane have been shown to offer anti-inflammatory effects during experimental endotoxemia whereas the alpha-adrenergic vasopressor norepinephrine exhibits proinflammatory properties on systemic cytokine release under the same conditions. However, during major surgery and in patients with systemic inflammatory response syndrome or sepsis both agents are frequently administered concurrently. We therefore aimed to investigate the influence of preexisting i.v. administration of noradrenaline or vasopressin on the anti-inflammatory effects of isoflurane during experimental endotoxemia. Anesthetized, ventilated Sprague-Dawley rats (n=7 per group) were randomly treated. In the LPS-only group, animals received lipopolysaccharide (LPS, 5 mg/kg, i.v.) with no further specific treatment. In the LPS-isoflurane group, isoflurane inhalation at 1 MAC was initiated simultaneously with induction of endotoxemia (LPS 5 mg/kg, i.v.). Animals in the LPS-isoflurane-norepinephrine group received norepinephrine infusion at 50 microg/kg/h 10 min prior to injection of LPS and inhalation of isoflurane. In the LPS-isoflurane-vasopressin group, vasopressin was administered at 0.5 IE/kg/h 10 min prior to LPS and isoflurane. In the LPS-norepinephrine and the LPS-vasopressin groups the infusion of each vasopressor was started prior to LPS injection without any application of isoflurane. A Sham group served as the control. After 4 h of endotoxemia, plasma levels of TNFalpha, IL-1beta and IL-10 were measured. Alveolar macrophages (AM) were cultured ex vivo for nitrite assay. Induction of endotoxemia resulted in a significant rise in measured plasma cytokines and nitrite production from cultured AM. Inhalation of isoflurane significantly attenuated plasma levels of TNFalpha (-65%) and IL-1beta (-53%) compared to the LPS-only group whereas it had no effect on nitrite production from cultured AM. Preexisting infusions of norepinephrine or vasopressin abolished the anti-inflammatory effects of isoflurane. The data demonstrate that the administration of norepinephrine or vasopressin both counteracted the anti-inflammatory effects of inhaled isoflurane on proinflammatory cytokine release during experimental endotoxemia in rats.
- Published
- 2007
26. Targeting caspase-1 by inhalation-therapy: effects of Ac-YVAD-CHO on IL-1 beta, IL-18 and downstream proinflammatory parameters as detected in rat endotoxaemia.
- Author
-
Boost KA, Hoegl S, Hofstetter C, Flondor M, Stegewerth K, Platacis I, Pfeilschifter J, Muhl H, and Zwissler B
- Subjects
- Administration, Inhalation, Aerosols, Animals, Blood Pressure drug effects, Cysteine Proteinase Inhibitors administration & dosage, Endotoxemia blood, Heart Rate drug effects, Male, Oligopeptides administration & dosage, Rats, Rats, Sprague-Dawley, Tumor Necrosis Factors metabolism, Caspase Inhibitors, Cysteine Proteinase Inhibitors therapeutic use, Endotoxemia drug therapy, Interleukin-18 blood, Interleukin-1beta blood, Oligopeptides therapeutic use
- Abstract
Objective: We set out to investigate whether the nebulized and inhaled specific caspase-1 inhibitor Ac-YVAD-CHO has the potential to attenuate the pulmonary and systemic release of the caspase-1-dependent cytokines interleukin-1 beta (IL-1 beta) and interleukin-18 (IL-18) as well as their downstream enzymes iNOS and COX-2 in rat experimental endotoxaemia., Design and Setting: Controlled, randomized animal study in a university research facility., Subject: Male Sprague-Dawley rats (n=32) were randomly treated as follows: Inhaled Ac-YVAD-CHO was administered in eight rats at a inhaled total dosage of 5 mg and in eight rats at a inhaled total dose of 0.5 mg before infusion of lipopolysaccharide (LPS; 5 mg/kg, i.v.). Eight animals received LPS only. Eight animals served as controls without endotoxaemia., Measurements and Results: After 4h of endotoxaemia, levels of IL-1 beta, IL-18 and TNF-alpha in plasma and bronchoalveolar fluid (BALF) were analyzed. Nitric oxide (NO) release from alveolar macrophages was measured by Griess assay. Amounts of iNOS protein in alveolar macrophages and COX-2 protein in lung homogenates were determined by Western blotting. Significant reductions in release of IL-1 beta (-58%, p<0.05) and IL-18 (-51%, p<0.05) in plasma and IL-1 beta (-59%, p<0.05) in BALF were found in animals pretreated with inhaled caspase-1 inhibitor compared with animals without therapy. Expression of iNOS in alveolar macrophages and COX-2 in lung tissue was concurrently decreased in the treatment groups compared with control animals., Conclusions: Our data demonstrate that administration of the caspase-1 inhibitor Ac-YVAD-CHO by inhalation is able to reduce the pulmonary and systemic release of proinflammatory mediators in rat endotoxaemia. These results further underscore that inhalation may constitute an effective route of anti-inflammatory drug administration, beneficial in the clinical setting of ARDS.
- Published
- 2007
- Full Text
- View/download PDF
27. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
- Author
-
Hofstetter C, Flondor M, Hoegl S, Muhl H, and Zwissler B
- Subjects
- Administration, Inhalation, Aerosols, Animals, Endotoxemia chemically induced, Interleukin-10 administration & dosage, Lipopolysaccharides, Male, Nebulizers and Vaporizers, Rats, Rats, Sprague-Dawley, Bronchoalveolar Lavage Fluid chemistry, Cytokines metabolism, Endotoxemia metabolism, Inflammation Mediators metabolism, Interleukin-10 pharmacology
- Abstract
Objective: We aimed to clarify if inhaled interleukin (IL)-10 attenuates pulmonary and systemic inflammation as indicated by reduced content of proinflammatory mediators in bronchoalveolar lavage fluid (BALF) and plasma in experimental endotoxemia in the rat., Design: Laboratory experiment., Setting: University research institute., Subjects: Anesthetized, ventilated rats (sd, 550 +/- 50 g)., Interventions: Rats were randomly treated as follows: Nebulized IL-10 (calculated deposition fraction, 0.1 microg/lung) was administered in eight rats before infusion of lipopolysaccharide (5 mg/kg, intravenously). Eight animals received the same insult with no further treatment. Eight rats served as controls without endotoxemia but with aerosolized saline., Measurements and Main Results: BALF and plasma levels of tumor necrosis factor (TNF)-alpha, IL-1beta, IL-6, interferon (IFN)-gamma, and RANTES were analyzed. Alveolar macrophages were cultured ex vivo for nitrite assay. In those animals treated with IL-10-aerosol, BALF levels of proinflammatory cytokines were reduced significantly compared with animals without IL-10 therapy (TNF-alpha, -87%; IL-1beta, -73%; IL-6, -44%; IFN-gamma, -39%; RANTES, -84%). In addition, nitrite release from cultured alveolar macrophages was suppressed by IL-10 inhalation (-96%). With the exception of TNF-alpha, similar results were observed for plasma levels of proinflammatory cytokines., Conclusions: The present data indicate that nebulized IL-10 reached the lungs in therapeutic effective concentrations and elicited anti-inflammatory effects on immunocompetent cells that are comparable to those already known from its intravenous administration in experimental endotoxemia.
- Published
- 2005
- Full Text
- View/download PDF
28. Aerosol delivery during mechanical ventilation to the rat.
- Author
-
Hofstetter C, Flondor M, Hoegl S, Thein E, Kemming G, Kisch-Wedel H, Kreyling W, and Zwissler B
- Subjects
- Animals, Blood Pressure, Fluorescent Dyes pharmacokinetics, Heart Rate, Lung Compliance, Male, Microspheres, Pulmonary Alveoli, Rats, Rats, Sprague-Dawley, Technetium, Aerosols, Nebulizers and Vaporizers, Respiration, Artificial instrumentation, Respiration, Artificial methods
- Abstract
The authors have adjusted a jet nebulizer to a mechanical ventilator (Servo Ventilator, Siemens) to deliver an aerosol to rats. They aimed to clarify whether a modified jet nebulizer generating particles with a mass median aerodynamic diameter of 2 microm would be effective and safe in intubated ventilated rats. Fluorescent microspheres (diameter: 1.0 microm) were aerosolized to verify qualitatively and quantitatively intrapulmonary deposition. Particle deposition fraction was 3.8% (1.3%) of the delivered dose (median [interquartile range]). There was no evidence for any adverse event as assessed from heart rate, mean arterial pressure, PaO2 and PaCO2 before, during, and after nebulization. No pulmonary tissue trauma was detected histologically., (Copyright Taylor & Francis Inc.)
- Published
- 2004
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.