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9 results on '"Hoffken, H"'

3. Crystal structure and enzyme kinetics of the (S)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain EbN1

Author

Hoffken, H. Wolfgang, Minh Duong, Friedrich, Thomas, Breuer, Michael, Hauer, Bernhard, Reinhardt, Richard, Rabus, Ralf, and Heider, Johann

Subjects
Escherichia coli -- Physiological aspects, Escherichia coli -- Research, Matter, Kinetic theory of -- Analysis, Crystals -- Structure, Crystals -- Research, Biological sciences, Chemistry
Abstract

The coding gene (ped) for (S)-1-phenylethanol dehydrogenase (PED) is cloned from strain EbN1 and heterologously overexpressed in Escherichia coli and the enzyme is purified and crystallized. The kinetic studies revealed a highly cooperative catalysis of the enzyme and strong regulation of its activity and a possible relation between the structural features and regulation of the enzyme if presented.

Published
2006

4. Parkinson's Disease is Overdiagnosed Clinically at Baseline in Diagnostically Uncertain Cases: A 3-Year European Multicenter Study with Repeat [I-123]FP-CIT SPECT

Author

Marshall, VL, Reininger, CB, Marquardt, M, Patterson, J, Hadley, DM, Oertel, WH, Benamer, HTS, Kemp, P, Burn, D, Tolosa, E, Kulisevsky, J, Cunha, L, Costa, D, Booij, J, Tatsch, K, Chaudhuri, KR, Ulm, G, Pogarell, O, Hoffken, H, Gerstner, A, and Grosset, DG

Subjects
SWEDD, Parkinson's disease, [I-123]FP-CIT, SPECT
Abstract

Over-diagnosis of Parkinson's disease (PD) is suggested by specialist review of community diagnosis, and in postmortem studies. In specialist centers 4 to 15% of patients entered into clinical trials as early PD do not have functional imaging support for a PD diagnosis. In a European multicenter prospective, longitudinal study, we compared clinical diagnosis with functional SPECT imging using [(123)-I]FP-CIT (DaTSCAN (TM). GE Healthcare). Repeat observations were performed over 3 years in patients with tremor and/or parkinsonism in whom there was initial diagnostic uncertainty between degenerative parkinsonism and nondegenerative tremor disorders. Video-recording of clinical features was scores independently of functional imaging results by two blinded clinicians at 36 months (= gold standard clincial diagnosis). Three readers, unaware of the clincial diagnosis, classified the images as normal or abnormal by visual inspection. The main endpoint was the sensitivity and specificity of SPECT imaging at baseline compared with the gold standard. In 99 patients completing the three serial assessments, on-site clinical diagnosis overdiagnosed degenerative parkinsonism at baseline in diagnostically uncertain cases compared with the gold standard clinical diagnosis (at 36 months), the latter giving sensitivity of 93% and specificity of 46%. The corresponding baseline [(123)-I]FP-CIT SPECT results showed a mean sensitivity of 78% and a specificity of 97%. Inter-reader agreement for rating scans as normal or abnormal was high (Cohen's (kappa) over cap = 0.94-0.97). (C) 2008 Movement Disorder Society

Published
2009

8. Tyrosine hydroxylase deficiency with severe clinical course: clinical and biochemical investigations and optimization of therapy.

Author

Dionisi-Vici C, Hoffmann GF, Leuzzi V, Hoffken H, Bräutigam C, Rizzo C, Steebergen-Spanjers GC, Smeitink JA, and Wevers RA

Subjects
Child, Preschool, Dopamine Agents administration & dosage, Dopamine Agents adverse effects, Drug Therapy, Combination, Humans, Levodopa administration & dosage, Levodopa adverse effects, Male, Metabolism, Inborn Errors metabolism, Monoamine Oxidase Inhibitors administration & dosage, Selegiline administration & dosage, Metabolism, Inborn Errors diagnosis, Metabolism, Inborn Errors drug therapy, Tyrosine 3-Monooxygenase deficiency
Abstract

Tyrosine hydroxylase deficiency was diagnosed after determination of cerebrospinal fluid neurotransmitters and DNA analysis in a child with severe axial hypotonia and hypokinesia associated with dystonic and ballistic movements. L-dopa therapy was unsuccessful, whereas a combination with selegiline, a selective monoamine oxidase-beta inhibitor, with low-dose L-dopa markedly improved the severe clinical picture.

Published
2000
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9. Embryogenesis and organogenesis of Carausius morosus under spaceflight conditions.

Author

Bucker H, Facius R, Horneck G, Reitz G, Graul EH, Berger H, Hoffken H, Ruther W, Heinrich W, Beaujean R, and Enge W

Subjects
Animals, Centrifugation, Gravitation, Insecta embryology, Insecta radiation effects, Larva growth & development, Larva radiation effects, Radiation Monitoring instrumentation, Radiometry, Regeneration, Abnormalities, Radiation-Induced, Cosmic Radiation, Insecta growth & development, Ovum radiation effects, Space Flight instrumentation, Weightlessness
Abstract

The influence of cosmic radiation and/or microgravity on insect development was studied during the 7 day German Spacelab Mission D1. Eggs of Carausius morosus of five stages differing in sensitivity to radiation and in capacity to regeneration were allowed to continue their development in the BIORACK 22 degrees C incubator, either at microgravity conditions or on the 1 g reference centrifuge. Using the Biostack concept--eggs in monolayers were sandwiched between visual track detectors--and the 1 g reference centrifuge, we were able to separate radiation effects from microgravity effects and also from combined effects of these two factors in space. After retrieval, hatching rates, growth kinetics and anomaly frequencies were determined in the different test samples. The early stages of development turned out to be highly sensitive to single hits of cosmic ray particles as well as to the temporary exposure to microgravity during their development. In some cases, the combined action of radiation and microgravity even amplified the effects exerted by the single parameters of space. Hits by single HZE particles caused early effects, such as body anomalies, as well as late effects, such as retarded growth after hatching. Microgravity exposure lead to a reduced hatching rate. A synergistic action of HZE particle hits and microgravity was established in the unexpectedly high frequency of anomal larvae. However, it cannot be excluded, that cosmic background radiation or low LET HZE particles are also causally involved in damage observed in the microgravity samples.

Published
1986
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