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16 results on '"Holland, Harry"'

1. Advancing wide implementation of precision oncology: A liquid nitrogen‐free snap freezer preserves molecular profiles of biological samples

Author

van der Wijngaart, Hanneke, primary, Jagga, Sahil, additional, Dekker, Henk, additional, de Goeij, Richard, additional, Piersma, Sander R., additional, Pham, Thang V., additional, Knol, Jaco C., additional, Zonderhuis, Babs M., additional, Holland, Harry J., additional, Jiménez, Connie R., additional, Verheul, Henk M. W., additional, Vanapalli, Srinivas, additional, and Labots, Mariette, additional

Published
2023
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2. Advancing wide implementation of precision oncology:A liquid nitrogen-free snap freezer preserves molecular profiles of biological samples

Author

van der Wijngaart, Hanneke, Jagga, Sahil, Dekker, Henk, de Goeij, Richard, Piersma, Sander R., Pham, Thang V., Knol, Jaco C., Zonderhuis, Babs M., Holland, Harry J., Jiménez, Connie R., Verheul, Henk M.W., Vanapalli, Srinivas, Labots, Mariette, van der Wijngaart, Hanneke, Jagga, Sahil, Dekker, Henk, de Goeij, Richard, Piersma, Sander R., Pham, Thang V., Knol, Jaco C., Zonderhuis, Babs M., Holland, Harry J., Jiménez, Connie R., Verheul, Henk M.W., Vanapalli, Srinivas, and Labots, Mariette

Abstract

Purpose: In precision oncology, tumor molecular profiles guide selection of therapy. Standardized snap freezing of tissue biospecimens is necessary to ensure reproducible, high-quality samples that preserve tumor biology for adequate molecular profiling. Quenching in liquid nitrogen (LN2) is the golden standard method, but LN2 has several limitations. We developed a LN2-independent snap freezer with adjustable cold sink temperature. To benchmark this device against the golden standard, we compared molecular profiles of biospecimens. Methods: Cancer cell lines and core needle normal tissue biopsies from five patients' liver resection specimens were used to compare mass spectrometry (MS)-based global phosphoproteomic and RNA sequencing profiles and RNA integrity obtained by both freezing methods. Results: Unsupervised cluster analysis of phosphoproteomic and transcriptomic profiles of snap freezer versus LN2-frozen K562 samples and liver biopsies showed no separation based on freezing method (with Pearson's r 0.96 (range 0.92–0.98) and >0.99 for K562 profiles, respectively), while samples with +2 h bench-time formed a separate cluster. RNA integrity was also similar for both snap freezing methods. Molecular profiles of liver biopsies were clearly identified per individual patient regardless of the applied freezing method. Two to 25 s freezing time variations did not induce profiling differences in HCT116 samples. Conclusion: The novel snap freezer preserves high-quality biospecimen and allows identification of individual patients' molecular profiles, while overcoming important limitations of the use of LN2. This snap freezer may provide a useful tool in clinical cancer research and practice, enabling a wider implementation of (multi-)omics analyses for precision oncology.

Published
2023

5. Development of a microminiature sorption cooler

Author

Burger, Johannes, Holland, Harry, terBrake, Marcel, Rogalla, Horst, and Wade, Larry

Subjects
Engineering (General)
Abstract

The development of a microcooler for operations below 80 K, for low temperature electronic devices requiring small cooling powers of the order of 10 mW is described. A sorption compressor combined with Joule-Thomson (JT) expansion was selected for miniaturization. The advantage of the system is considered to be its absence of moving parts, with the exception of the check valves. This facilitates the scaling down of the system, minimizes interferences and contributes to a long lifetime. The requirements for small scale cooling of low temperature electronics are listed. The sorption compressor and the JT expansion are described. A parameter study is reviewed together with the scaling of the sorption compressor.

Published
1997

16. Long-life micro vacuum chamber for a micromachined cryogenic cooler.

Author

Haishan Cao, Vermeer, Cristian H., Vanapalli, Srinivas, Holland, Harry J., and ter Brake, H. J. Marcel

Subjects
VACUUM chambers, MICROELECTROMECHANICAL systems, CRYOGENICS, COOLING of electronic appliances, THERMAL insulation, STAINLESS steel
Abstract

Micromachined cryogenic coolers can be used for cooling small electronic devices to improve their performance. However, for reaching cryogenic temperatures, they require a very good thermal insulation from the warm environment. This is established by a vacuum space that for adequate insulation has to be maintained at a pressure of 0.01 Pa or lower. In this paper, the challenge of maintaining a vacuum chamber with a volume of 3.6 × 10-5 m³ and an inner wall area of 8.1 × 10-3 m² at a pressure no higher than 0.01 Pa for five years is theoretically analyzed. The possible sources of gas, the mechanisms by which these gases enter the vacuum space and their effects on the pressure in the vacuum chamber are discussed. In a long-duration experiment with four stainless steel chambers of the above dimensions and equipped with a chemical getter, the vacuum pressures were monitored for a period of two years. In that period, the measured pressure increase stayed within 0.01 Pa. This study can be used to guide the design of long-lifetime micro vacuum chambers that operate without continuous mechanical pumping. [ABSTRACT FROM AUTHOR]

Published
2015
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