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31 results on '"Hong, Yean Ju"'

17. Inhibition of neural stem cell aging through the transient induction of reprogramming factors.

Author

Han, Min Ji, Lee, Won Ji, Choi, Joonhyuk, Hong, Yean Ju, Uhm, Sang Jun, Choi, Youngsok, and Do, Jeong Tae

Abstract

Adult stem cells age during long‐term in vitro culture, and neural stem cells (NSCs), which can self‐renew and differentiate into neurons and glial cells, also display reduced differentiation potential after repeated passaging. However, the mechanistic details underlying this process remain unclear. In this study, we found that long‐term in vitro culture of NSCs resulted in aging‐related upregulation of inflammatory‐ and endoplasmic reticulum (ER) stress‐related genes, including the proinflammatory cytokines interleukin (IL)1β and IL6, the senescence‐associated enzyme matrix metallopeptidase 13 (MMP13), and the ER stress‐responsive transcription factor activating transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP). However, the cyclic and transient induction of four reprogramming factors (POU domain, class 5, transcription factor 1, also known as octamer‐binding transcription factor 4; SRY [sex determining region Y]‐box 2; Kruppel‐like factor 4; and myelocytomatosis oncogene; collectively referred to as OSKM) can inhibit NSC aging, as indicated by the decreased expression of the inflammatory and ER stress‐related genes. We used ROSA‐4F NSCs, which express OSKM from only one allele, to minimize the potential for full reprogramming or tumor formation during NSC rejuvenation. We expect that this novel rejuvenation method will enhance the potential of NSCs as a clinical approach to the treatment of neurological diseases. [ABSTRACT FROM AUTHOR]

Published
2021
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26. Mitochondrial and Metabolic Remodeling During Reprogramming and Differentiation of the Reprogrammed Cells

Author

Choi, Hyun Woo, primary, Kim, Jin Hoi, additional, Chung, Mi Kyung, additional, Hong, Yean Ju, additional, Jang, Hyun Sik, additional, Seo, Bong Jong, additional, Jung, Taek Hee, additional, Kim, Jong Soo, additional, Chung, Hyung Min, additional, Byun, Sung June, additional, Han, Sung Gu, additional, Seo, Han Geuk, additional, and Do, Jeong Tae, additional

Published
2015
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30. Cellular Reprogramming Using Protein and Cell-Penetrating Peptides.

Author

Seo BJ, Hong YJ, and Do JT

Subjects
Animals, Cell Lineage, Cell Membrane Permeability, Cell Transdifferentiation, Cell-Penetrating Peptides genetics, Cellular Reprogramming Techniques, Humans, Protein Transport, Proteins genetics, Transcription Factors genetics, Cell-Penetrating Peptides metabolism, Cellular Reprogramming, Induced Pluripotent Stem Cells metabolism, Proteins metabolism, Transcription Factors metabolism
Abstract

Recently, stem cells have been suggested as invaluable tools for cell therapy because of their self-renewal and multilineage differentiation potential. Thus, scientists have developed a variety of methods to generate pluripotent stem cells, from nuclear transfer technology to direct reprogramming using defined factors, or induced pluripotent stem cells (iPSCs). Considering the ethical issues and efficiency, iPSCs are thought to be one of the most promising stem cells for cell therapy. Induced pluripotent stem cells can be generated by transduction with a virus, plasmid, RNA, or protein. Herein, we provide an overview of the current technology for iPSC generation and describe protein-based transduction technology in detail.

Published
2017
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31. Generation of Partially Reprogrammed Cells and Fully Reprogrammed iPS Cells by Plasmid Transfection.

Author

Kim JS, Choi HW, Hong YJ, and Do JT

Subjects
Animals, Cell Culture Techniques methods, Cells, Cultured, Chimera genetics, Embryo Culture Techniques methods, Female, Fibroblast Growth Factor 2 pharmacology, Genes, Reporter, Homeodomain Proteins genetics, Homeodomain Proteins physiology, Kruppel-Like Factor 4, Kruppel-Like Transcription Factors genetics, Kruppel-Like Transcription Factors physiology, Male, Mice, Nanog Homeobox Protein, Octamer Transcription Factor-3 genetics, Octamer Transcription Factor-3 physiology, Proto-Oncogene Proteins c-myc genetics, Proto-Oncogene Proteins c-myc physiology, SOXB1 Transcription Factors genetics, SOXB1 Transcription Factors physiology, Transfection, Cellular Reprogramming drug effects, Cellular Reprogramming Techniques methods, Genetic Vectors genetics, Induced Pluripotent Stem Cells cytology, Plasmids genetics
Abstract

Induced pluripotent stem (iPS) cells can be directly generated from somatic cells by overexpression of defined transcription factors. iPS cells can perpetually self-renew and differentiate into all cell types of an organism. iPS cells were first generated through infection with retroviruses that contain reprogramming factors. However, development of an exogene-free iPS cell generation method is crucial for future therapeutic applications, because integrated exogenes result in the formation of tumors in chimeras and regain pluripotency after differentiation in vitro. Here, we describe a method to generate iPS cells by transfection of plasmid vectors and to convert partially reprogrammed cells into fully reprogrammed iPS cells by switching from mouse ESC culture conditions to KOSR-based media with bFGF. We also describe basic methods used to characterize fully reprogrammed iPS cells.

Published
2016
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