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Despite the fact that low-dimensional carbons (LDCs, 1D/2D) materials are very interesting due to their intriguing electrical properties, we still attempt to enrich them by high N-content in order to enjoy their electro-applications. We here report a template-free synthesis of 1D/2D LDC with high N content (>40 ​at%) and tunable aspect ratios from molecular formamide (FA). The 1D/2D LDC is in polyaminoimidazole as confirmed by pair distribution function analysis, and 1D growth mode can be altered to 2D by simply adding a 2D-guiding molecule of melamine. Electrochemical properties of the LDC can be finely tuned by adjusting the solvothermal temperature and melamine dosage. It is revealed that the optimal 2D LDC delivers superior O2-to-H2O2 yield (687.2 ​mmol·g−1⋅h−1) and Faradic efficiency (87.5%). Considering the heavy N content and high adjustability of aspect ratio, the FA-derived LDCs potentially open new synthesis routes for structural carbon materials for broad electrochemical applications.

The insulation co-ordination of converter transformer winding has a great influence on power system security. An equivalent circuit of a 500 kV converter transformer winding structure, including a LC equivalent network circuit and an ideal transformer in parallel, was firstly established. A combined 12-pulse rectifier equivalent circuit model, mainly consisting of six single-phase converter transformer and 12 converter valves, was built in Power Systems Computer Aided Design (PSCAD) software. In certain operating states of the converter value, the transient voltage distribution was analysed when lightning strokes the DC bus of a typical converter station. The results show that the dual winding model can present the transient voltage characteristics of the converter transformer. The maximum lightning transient voltage is located at the end of the star-connected winding near the neutral point and reaches 1270 kV, and also the maximum gradient voltage occurs at the winding near the terminal point. In different operating states for the converter valve, the transient voltages of a three-phase winding behave as per the similar distributing rules. For the star-connected phase winding, the lightning transient voltage of the winding disc reaches the maximum magnitude when lightning strike occurs at the peak of the phase voltage on the valve side.

A strong environmental regulatory framework enhances green technology innovation (GTI), which is crucial for sustainable economic growth. We construct SDM models by using panel data from 108 cities in China’s Yangtze River Economic Belt (YREB) from 2011 to 2020 to investigate the effects of heterogeneous environmental regulations (ER) on GTI in local and neighboring cities. The moderating influence of digital financial inclusion (DFI) is also examined within the SDM model. Our findings reveal that: (1) Different types of ER have varying impacts on GTI. (2) Command-and-control environmental regulation (CER) hinders local GTI but promotes GTI in neighboring cities. Both market-based (MER) and public-participation environmental regulations (PER) promote GTI in both local and neighboring cities. (3) DFI positively moderates the impact of ER on GTI by providing flexible finance support to enterprises. The study concludes with policy recommendations to improve environmental regulation systems, enhance regional synergistic governance, and promote digital financial inclusion for green sustainable development.

To improve the high-value utilization of the coffee processing by-product, the coffee peel insoluble dietary fiber (CPIDF) was modified by wet ball milling, the effects of ball milling time on the composition, structure and functional properties of the CPIDF was investigated. The results showed that the soluble dietary fiber (SDF) content of CPIDF increased significantly (P

Abstract Background As one of the most important staple food crops, rice produces large of agronomic biomass residues that contain lots of secondary cell walls (SCWs). Membrane trafficking plays key roles in SCWs biosynthesis, but information association membrane trafficking and SCWs formation in plants is limited. Results In this study, we report the function characterization of a rice mutant, c ulm e asily f ragile 3 (cef3), that exhibits growth retardation and fragile culm phenotype with significantly altered cell wall composition and reduced secondary wall thickness. Map-based cloning revealed that CEF3 encodes a homologous protein of Arabidopsis STOMATAL CYTOKINESIS DEFECTIVE2 (SCD2). The saccharification assays revealed that CEF3 mutation can improve biomass enzymatic saccharification. Expression pattern analysis indicated that CEF3 is ubiquitously expressed in many organs at different developmental stages. Subcellular localization revealed that CEF3 is a Golgi-localized protein. The FM4-64 uptake assay revealed CEF3 is involved in endocytosis. Furthermore, mutation of CEF3 not only affected cellulose synthesis-related genes expression, but also altered the abundance of cellulose synthase catalytic subunit 9 (OsCESA9) in the PM and in the endomembrane systems. Conclusions This study has demonstrated that CEF3 participates in the membrane trafficking that is essential for normal cellulose and other polysaccharides biosynthesis of the secondary cell wall, thereby manipulation of CEF3 could alter cellulose content and enhance biomass enzymatic saccharification in rice plants. Therefore, the study of the function of CEF3 can provide a strategy for genetic modification of SCWs in bioenergy crops.

Grain weight is a major determinant in rice yield, which is tightly associated with grain size. However, the underlying molecular mechanisms that control this trait remain unclear. Here, we report a rice (Oryza sativa) mutant, low grain weight (lgw), which shows that reduced grain length is caused by decreased cell elongation and proliferation. Map-based cloning revealed that all mutant phenotypes resulted from a nine-base pair (bp) deletion in LGW, which encodes the kinesin-like protein BRITTLE CULM12 (BC12). Protein sequence alignment analysis revealed that the mutation site was located at the nuclear localization signal (NLS) of LGW/BC12, resulting in the lgw protein not being located in the nucleus. LGW is preferentially expressed in both culms and roots, as well as in the early developing panicles. Overexpression of LGW increased the grain length, indicating that LGW is a positive regulator for regulating grain length. In addition, LGW/BC12 is directly bound to the promoter of GW7 and activates its expression. Elevating the GW7 expression levels in lgw plants rescued the small grain size phenotype. We conclude that LGW regulates grain development by directly binding to the GW7 promoter and activating its expression. Our findings revealed that LGW plays an important role in regulating grain size, and manipulation of this gene provides a new strategy for regulating grain weight in rice.

Abstract Background Cellulose synthase (CESA) mutants have potential use in straw processing due to their lower cellulose content, but almost all of the mutants exhibit defective phenotypes in plant growth and development. Balancing normal plant growth with reduced cellulose content remains a challenge, as cellulose content and normal plant growth are typically negatively correlated with one another. Result Here, the rice (Oryza sativa) semi-dominant brittle culm (sdbc) mutant Sdbc1, which harbors a substitution (D387N) at the first conserved aspartic acid residue of OsCESA9, exhibits lower cellulose content and reduced secondary wall thickness as well as enhanced biomass enzymatic saccharification compared with the wild type (WT). Further experiments indicated that the OsCESA9D387N mutation may compete with the wild-type OsCESA9 for interacting with OsCESA4 and OsCESA7, further forming non-functional or partially functional CSCs. The OsCESA9/OsCESA9D387N heterozygous plants increase salt tolerance through scavenging and detoxification of ROS and indirectly affecting related gene expression. They also improve rice straw return to the field due to their brittle culms and lower cellulose content without any negative effects in grain yield and lodging. Conclusion Hence, OsCESA9D387N allele can improve rice salt tolerance and provide the prospect of the rice straw for biofuels and bioproducts due to its improved enzymatic saccharification.

Silkworm pupa oil (SPO) contains unsaturated fatty acids, tocopherols, and phytosterols, which can regulate serum total cholesterol or be used as an antioxidant. In this study, we investigated the impacts of SPO on the antioxidant stress and lipid metabolism of Caenorhabditis elegans. The lifespan of the C. elegans fed with different SPO concentrations was determined. The levels of endogenous reactive oxygen species (ROS) were analyzed with the fluorescent probe method. The activity of antioxidant enzymes and the content of malondialdehyde (MDA) were analyzed. The transcription level of specific mRNA was characterized with q-PCR. The survival time of the mutant strain under oxidative stress was determined by daf-2 (CB1370) mutant, sod-3 (GA186) mutant, and skn-1 (EU31) mutant. As for the lipid metabolism, the lipid accumulation was determined with an Oil-Red-O (ORO) staining. The transcription level of specific mRNA was determined by q-PCR. The results showed that the SPO feeding enhanced the activities of antioxidant enzyme by upregulating the expression of the genes skn-1, and sod-3 to decrease the production of ROS and MDA, which prolonged the life of nematodes treated with juglone. ORO staining analysis indicated the feeding of SPO decreased intestinal fat accumulation, downregulated expression of fat-5, fat-6, fat-7, and nhr-80, and upregulated age-1 and tph-1 expression. Conclusively, SPO enhanced the antioxidant capacity by regulating the skn-1 and sod-3 expression of antioxidant gene and reducing the fat accumulation by the insulin/IGF signaling pathway and nuclear hormone receptor nhr-80 signaling pathway of nematodes. This study provides new evidence for the antioxidant and lipid-lowering mechanisms of SPO in C. elegans.

Commercially available biomedical wearable sensors to measure tensile force/strain still struggle with miniaturization in terms of weight, size, and conformability. Flexible and epidermal electronic devices have been utilized in these applications to overcome these issues. However, current sensors still require a power supply and some form of powered data transfer, which present challenges to miniaturization and to applications. Here, we report on the development of flexible, passive (thus zero power consumption), and biocompatible nanostructured photonic devices that can measure tensile strain in real time by providing an optical readout instead of an electronic readout. Hierarchical silver (Ag) nanostructures in various thicknesses of 20–60 nm were fabricated and embedded on a stretchable substrate using e-beam lithography and a low-temperature dewetting process. The hierarchical Ag nanostructures offer more design flexibility through a two-level design approach. A tensional force applied in one lateral (x- or y-) direction of the stretchable substrate causes a Poisson contraction in the other, and as a result, a shift in the reflected light of the nanostructures. A clear blue shift of more than 100 nm in peak reflectance in the visible spectrum was observed in the reflected color, making the devices applicable in a variety of biomedical photonic sensing applications.

The low reactivity of lignin hinders its application as a phenol substitute in phenol–formaldehyde (PF) resin. Therefore, the combination of fractionation and phenolation was adopted to enhance the reactivity of lignin for preparing a phenol–formaldehyde resin adhesive. Sugarcane bagasse kraft lignin and its fractions were employed to replace 40 wt% of phenol to prepare a PF adhesive. The fractionation increased the reactivity of lignin, however the as-prepared lignin-based PF (LPF) hardly met its application requirements as an adhesive. Therefore, the phenolation of lignin under an acidic condition was adopted to further improve its reactivity. The phenolated lignin was characterized by FTIR, gel permeation chromatography, and NMR, indicating its active sites increased while its molecular weight decreased. The phenolated lignin was used to replace 40 wt% of phenol to prepare a PF adhesive (PLPF) which was further employed to prepare plywood. The results indicated that the combination of fractionation and phenolation effectively enhanced the reactivity of lignin, and eventually improved the properties of the PLPF and its corresponding plywood. The free formaldehyde content of PLPF decreased to 0.16%. The wet bonding strength of the as-prepared plywood increased to 1.36 MPa, while the emission of formaldehyde decreased to 0.31 mL/L.

Existing laparoscopic surgery systems use a single laparoscope to visualize the surgical area with a limited field of view (FoV), necessitating maneuvering the laparoscope to search a target region. In some cases, the laparoscope needs to be moved from one surgical port to another one to detect target organs. These maneuvers would cause longer surgical time and degrade the efficiency of operation. We hypothesize that if an array of cameras can be deployed to provide a stitched video with an expanded FoV and small blind spots, the time required to perform multiple tasks at different sites can be significantly reduced. We developed a micro-camera array that can enlarge the FoV and reduce blind spots between the cameras by optimizing the angle of cameras. The video stream of this micro-camera array was designed to be processed in real-time to provide a stitched video with the expanded FoV. We mounted this micro-camera array to a Fundamentals of Laparoscopic Surgery (FLS) laparoscopic trainer box and designed an experiment to validate the hypothesis above. Surgeons, residents, and a medical student were recruited to perform a modified bean drop task, and the completion time was compared against that measured using a traditional single-camera laparoscope. It was observed that utilizing the micro-camera array, the completion time of the modified bean drop task was 203 ± 55 s while using the laparoscope, the completion time was 245 ± 114 s, with a p-value of 0.00097. It is also observed that the benefit of using an FoV-expanded camera array does not diminish for subjects who are more experienced. This test provides convincing evidence and validates the hypothesis that expanded FoV with small blind spots can reduce the operation time for laparoscopic surgical tasks.

MnO2-deposited lignin-based carbon fiber (MnO2-LCF) mats are fabricated for supercapacitor applications. LCF mats are produced from alkali lignin via electrospinning followed by stabilization and carbonization. The carbonization process is carried out at 800, 900, and 1000 °C, and the corresponding mats are denoted as MnO2-LCF-800, MnO2-LCF-900, and MnO2-LCF-1000, respectively. The LCF mats are immersed in a KMnO4 solution at room temperature for 72 h to obtain MnO2-LCF mats. The scanning electron microscopy and X-ray diffraction analysis confirm the deposition of MnO2 on the LCFs. The Brunauer−Emmett−Teller analysis, X-ray spectroscopy, and Raman spectroscopy reveal that MnO2-LCF-800 mat possesses a large number of mesopores and Mn vacancies as compared to MnO2-LCF-900 mat and MnO2-LCF-1000 mat. Consequently, MnO2-LCF-800 mat possesses the best electrochemical properties with a specific capacitance of 131.28 F∙g−1, an energy density of 14.77 Wh∙kg−1, and a power density of 135.01 W∙kg−1 at a specific current of 0.3 A∙g−1. Hence, MnO2-LCF-800 mat shows high potential to be used as a high-performance supercapacitor.

We present a flexible variable-focus converging microlens actuated by electrowetting on dielectric (EWOD). The microlens is made of two immiscible liquids and a soft polymer, polydimethylsiloxane (PDMS). Parylene intermediate layer is used to produce robust flexible electrode on PDMS. A low-temperature PDMS-compatible fabrication process has been developed to reduce the stress on the lens structure. The lens has been demonstrated to be able to conform to curved surfaces smoothly. The focal length of the microlens is 29–38 mm on a flat surface, and 31–41 mm on a curved surface, varying with the voltage applied. The resolving power of the microlens is 25.39 line pairs per mm by a 1951 United States Air Force (USAF) resolution chart and the lens aberrations are measured by a Shack-Hartmann wavefront sensor. The focal length behavior on a curved surface is discussed and for the current lens demonstrated the focal length is slightly longer on the curved surface as a result of the effect of the curved PDMS substrate.

We present a wide-angle, broad-spectrum cylindrical lens based on reflections from an array of three-dimensional, high-aspect-ratio micro-mirrors fabricated on a cylindrical elastomeric substrate, functionally inspired by natural reflecting superposition compound eyes. Our device can perform one-dimensional focusing and beam-shaping comparable to conventional refraction-based cylindrical lenses, while avoiding chromatic aberration. The focal length of our cylindrical lens is 1.035 mm, suitable for micro-optical systems. Moreover, it demonstrates a wide field of view of 152° without distortion, as well as modest spherical aberrations. Our work could be applied to diverse applications including laser diode collimation, barcode scanning, holography, digital projection display, microlens arrays, and optical microscopy.

We report on polydimethlysiloxane (PDMS) microlenses and microlens arrays on flat and curved substrates fabricated via a relatively simple process combining liquid-phase photopolymerization and a single molding step. The mold for the formation of the PDMS lenses is fabricated by photopolymerizing a polyacrylamide (PAAm) pre-hydrogel. The shrinkage of PAAm after its polymerization forms concave lenses. The lenses are then transferred to PDMS by a single step molding to form PDMS microlens array on a flat substrate. The PAAm concave lenses are also transferred to PDMS and another flexible polymer, Solaris, to realize artificial compound eyes. The resultant microlenses and microlens arrays possess good uniformity and optical properties. The focal length of the lenses is inversely proportional to the shrinkage time. The microlens mold can also be rehydrated to change the focal length of the ultimate PDMS microlenses. The spherical aberration is 2.85 μm and the surface roughness is on the order of 204 nm. The microlenses can resolve 10.10 line pairs per mm (lp/mm) and have an f-number range between f/2.9 and f/56.5. For the compound eye, the field of view is 113°.

Tunable-focus liquid lenses provide focal length tuning for optical systems, e.g., cameras, where physical movement of rigid lenses are not an option or not preferable. In this work we present a magnetically actuated liquid lens utilizing the Lorentz force to vary the focal length as the current through the system is varied. The resulting lens can operate as both a diverging and a converging lens depending on the direction of current applied and has a large range of focal lengths, from −305 mm to –111 mm and from 272 mm to 146 mm. We also characterized the aberrations of the lens during the actuation with a Shack–Hartmann wavefront sensor, and utilized the lens for imaging, during which we measured a resolution of 7.13 lp/mm.

We have fabricated a fully-flexible, focus-tunable microlens array on a sheet and demonstrated its imaging capabilities. Each liquid lens of the array is individually tunable via electrowetting on dielectric (EWOD) actuation and is situated on a polydimethylsiloxane (PDMS) substrate, which allows the lens array to operate as a reconfigurable optical system. In particular, we observed a significant increase in the field of view (FOV) of the system to 40.4° by wrapping it on a cylindrical surface as compared to the FOV of 21.5° obtained by the array on a planer surface. We also characterized the liquid lenses of the system, observing a range of focus length from 20.2 mm to 9.2 mm as increased voltage was applied to each EWOD lens. A Shack−Hartmann wavefront sensor (SHWS) was used to measure the wavefront of the lens as it was actuated, and the aberrations of the lens were assessed by reporting the Zernike coefficients of the wavefronts.

In this paper, we propose a novel multi-view image denoising algorithm based on convolutional neural network (MVCNN). Multi-view images are arranged into 3D focus image stacks (3DFIS) according to different disparities. The MVCNN is trained to process each 3DFIS and generate a denoised image stack that contains the recovered image information for regions of particular disparities. The denoised image stacks are then fused together to produce a denoised target view image using the estimated disparity map. Different from conventional multi-view denoising approaches that group similar patches first and then perform denoising on those patches, our CNN-based algorithm saves the effort of exhaustive patch searching and greatly reduces the computational time. In the proposed MVCNN, residual learning and batch normalization strategies are also used to enhance the denoising performance and accelerate the training process. Compared with the state-of-the-art single image and multi-view denoising algorithms, experiments show that the proposed CNN-based algorithm is a highly effective and efficient method in Gaussian denoising of multi-view images.

The quality and the extent of intra-abdominal visualization are critical to a laparoscopic procedure. Currently, a single laparoscope is inserted into one of the laparoscopic ports to provide intra-abdominal visualization. The extent of this field of view (FoV) is rather restricted and may limit efficiency and the range of operations. Here we report a trocar-camera assembly (TCA) that promises a large FoV, and improved efficiency and range of operations. A video stitching program processes video data from multiple miniature cameras and combines these videos in real-time. This stitched video is then displayed on an operating monitor with a much larger FoV than that of a single camera. In addition, we successfully performed a standard and a modified bean drop task, without any distortion, in a simulator box by using the TCA and taking advantage of its FoV which is larger than that of the current laparoscopic cameras. We successfully demonstrated its improved efficiency and range of operations. The TCA frees up a surgical port and potentially eliminates the need of physical maneuvering of the laparoscopic camera, operated by an assistant.

An optimal camera placement problem is investigated. The objective is to maximize the area of the field of view (FoV) of a stitched video obtained by stitching video streams from an array of cameras. The positions and poses of these cameras are restricted to a given set of selections. The camera array is designed to be placed inside the abdomen to support minimally invasive laparoscopic surgery. Hence, a few non-traditional requirements/constraints are imposed: Adjacent views are required to overlap to support image registration for seamless video stitching. The resulting effective FoV should be a contiguous region without any holes and should be a convex polygon. With these requirements, traditional camera placement algorithms cannot be directly applied to solve this problem. In this work, we show the complexity of this problem grows exponentially as a function of the problem size, and then present a greedy polynomial time heuristic solution that approximates well to the globally optimal solution. We present a new approach to directly evaluate the combined coverage area (area of FoV) as the union of a set of quadrilaterals. We also propose a graph-based approach to ensure the stitching requirement (overlap between adjacent views) is satisfied. We present a method to find a convex polygon with maximum area from a given polygon. Several design examples show that the proposed algorithm can achieve larger FoV area while using much less computing time.

Electrowetting-on-dielectric (EWOD) is a fast, well-established actuation method for a variety of applications, from microfluidics to electrowetting displays to electrowetting lenses. We therefore seek to develop a robust, scalable fabrication method for the realization of EWOD on a flexible polydimethylsiloxane (PDMS) substrate in order to increase the range of possible applications. We fabricated a 5 × 5 array of individually controlled electrowetting cells to manipulate silicone oil droplets via EWOD. The fabrication process utilized exclusively flexible materials to improve the robustness of the overall device, and processing methods were adapted to accommodate the particular challenges posed by flexible materials. Simulation of the EWOD devices was conducted using ANSYS Fluent and showed the change in contact angle in response to voltage applied. Fabricated devices were also tested, with actuation of the oil droplet observed with up to 100 V (RMS) AC applied across underlying electrodes. We demonstrated fabrication of a fully flexible array and verified actuation to center droplets over the electrodes. This work may be expanded to address more specific flexible applications for EWOD.

The study and application of microscale lenses and lens arrays have been actively researched in recent years; new approaches in the fabrication of microlenses and microlens arrays have emerged. Also, novel applications of these microlenses and microlens arrays have been demonstrated. In an effort to disseminate the current advances in this specialized field of microlenses and microlens arrays, and to encourage discussion on the future research directions while stimulating research interests in this area, a Special Issue of Micromachines has been dedicated to “Microlenses”. [...]

Harvesting and storing solar energy has become more and more important. Current solid-state photovoltaic cells and conventional photoelectrochemical cells are not capable of directly storing the converted energy, which has to be facilitated by connecting to external storing devices. We demonstrate a device architecture that can convert and store solar energy in the electrical form within an intrinsically single structure. Mobile charge is internally stored, based on the coupling between photoelectrochemical and ferroelectric effects. The tested device architecture can be photo-charged under 1000 W/m2 of white light to an open-circuit voltage of 0.47V with a capacity of 37.62 mC/cm2. After removal of the light source, the mobile charge stored lasts more than 8 hours, and the open-circuit output voltage lasts more than 24 hours.