99 results on '"Hosoda, R."'
Search Results
2. SIRT1 protects the heart against doxorubicin-induced cardiotoxicity by mediating the DNA damage response via deacetylation of histone H2AX
- Author
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Kuno, A, primary, Hosoda, R, additional, and Horio, Y, additional
- Published
- 2021
- Full Text
- View/download PDF
3. Seakeeping [and Discussion]
- Author
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Hosoda, R., Robinson, D. W., Nicholson, K., and Victory, G.
- Published
- 1991
4. The histone deacetylase SIRT1 mediates the proper DNA repair response by targeting histone H2AX to protect against doxorubicin-induced cardiotoxicity
- Author
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Kuno, A, primary, Hosoda, R, additional, and Horio, Y, additional
- Published
- 2020
- Full Text
- View/download PDF
5. Rearing of a coleopterous predator,Trogossita japonica (Col.: Trogossitidae), on artificial diets
- Author
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Ogura, N. and Hosoda, R.
- Published
- 1995
- Full Text
- View/download PDF
6. Semiconductor Nanostructure Design for Thermoelectric Property Control
- Author
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Nakamura, Y., primary, Ishibe, T., additional, Taniguchi, T., additional, Terada, T., additional, Hosoda, R., additional, and Sakane, Sh., additional
- Published
- 2019
- Full Text
- View/download PDF
7. Effect of (S)-α-fluoromethylhistidine and SMS 201-995 on growth of histamine-producing gastric carcinoid ofMastomys natalensis
- Author
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Hosoda, S., Hosoda, R., and Konishi, T.
- Published
- 1993
- Full Text
- View/download PDF
8. Cytosolic SIRT3 protects PC12 cells against amyloid beta toxicity
- Author
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Matsushita, T., primary, Hayashi, T., additional, Hosoda, R., additional, Kuno, A., additional, Iwahara, N., additional, Hisahara, S., additional, Shimohama, S., additional, and Horio, Y., additional
- Published
- 2017
- Full Text
- View/download PDF
9. DNIC responses evoked by chemical or mechanical craniofacial conditioning stimuli in healthy men and women
- Author
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Bagues, A., Lars Arendt-Nielsen, Martin, M. I., Hosoda, R., and Kelun Wang
- Published
- 2010
10. Technical feasibility of "Eco-Platform" - technology integration for the 21st century
- Author
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Hosoda, R., primary, Murota, S., additional, and Nakatani, S., additional
- Published
- 2003
- Full Text
- View/download PDF
11. Application of marine radar for ocean wave monitoring.
- Author
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Kojima, T., Hosoda, R., Susaki, H., Hiraoka, Y., Iwasaki, Y., and Yamada, T.
- Published
- 2004
- Full Text
- View/download PDF
12. ANGIOTENSIN - CONVERTING ENZYME GENE POLYMORPHISM IN PATIENTS WITH NEUROPATHIC PAIN
- Author
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Kimura, T., primary, Komatsu, T., additional, Hosoda, R., additional, Nishiwaki, K., additional, and Shimada, Y., additional
- Published
- 1998
- Full Text
- View/download PDF
13. 59 Characterization of presenilins overexpressed in culture cells
- Author
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Tomita, T., primary, Maruyama, K., additional, Saido, T.C., additional, Hosoda, R., additional, Tokuhiro, S., additional, Kume, H., additional, Kuzume, H., additional, Asada, H., additional, Kawamura, Y., additional, Iwatsubo, T., additional, and Obata, K., additional
- Published
- 1996
- Full Text
- View/download PDF
14. 703 Detection of isoaspartyl form of amyloid β protein in Aizheimer's disease brains
- Author
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Hosoda, R., primary, Saido, T.C., additional, Otvos, L., additional, Arai, T., additional, and Iwatsubo, T., additional
- Published
- 1996
- Full Text
- View/download PDF
15. Favorable effects of epidural analgesia on hemodynamics, oxygenation and metabolic variables in the immediate post-anesthetic period
- Author
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HOSODA, R., primary, HATTORI, M., additional, and SHIMADA, Y., additional
- Published
- 1993
- Full Text
- View/download PDF
16. A809 REGIONAL INTRAVENOUS INJECTION OF Ca-ANTAGONIST IN THE TREATMENT OF RAYNAUD'S SYNDROME AND REFLEX SYMPATHETIC DYSTROPHY
- Author
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Kawanishi, M., primary, Hosoda, R., additional, Kurokawa, Y., additional, Enomoto, A., additional, and Shimada, Y., additional
- Published
- 1990
- Full Text
- View/download PDF
17. Rearing of a coleopterous predator, Trogossita japonica (Col.: Trogossitidae) , on artificial diets
- Author
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Ogura, N. and Hosoda, R.
- Abstract
Abstract: Artificial diets for a coleopterous predator of pine bark and wood borers,Trogossita japonica Reitter (Col.: Trogossitidae) were studied. Diets composed of silkworm pupa-powder, dry yeasts, sucrose, peptone, squid liver oil, agar and distilled water supported larval development. The size of diet-reared adults was smaller than that in the control but they mated and oviposited normally when maintained on prey insects. An aseptic rearing method for the larvae of predator, that required a single diet change, was devised.
- Published
- 1995
- Full Text
- View/download PDF
18. Construction and testing of JT-60
- Author
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Kishimoto, H., primary, Aikawa, H., additional, Oikawa, A., additional, Miya, N., additional, Suzuki, K., additional, Ozeki, T., additional, Tokutake, T., additional, Kunieda, S., additional, Hiruta, K., additional, and Hosoda, R., additional
- Published
- 1987
- Full Text
- View/download PDF
19. Initial experiments in JT-60
- Author
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Abe, T, primary, Aikawa, H, additional, Akaoka, N, additional, Akasaka, H, additional, Akino, N, additional, Akiyama, T, additional, Ando, T, additional, Anno, K, additional, Aoyagi, T, additional, Arai, T, additional, Arakawa, K, additional, Azumi, M, additional, Fukuda, T, additional, Furukawa, H, additional, Hammamatsu, K, additional, Haraguchi, T, additional, Hayashi, K, additional, Hiratsuka, H, additional, Hirayama, T, additional, Hiroki, S, additional, Hiruta, K, additional, Hitomi, N, additional, Hosoda, R, additional, Hosogane, N, additional, Ichige, H, additional, Iida, S, additional, Iijima, T, additional, Ikeda, Y, additional, Isaji, N, additional, Isaka, M, additional, Ishihara, M, additional, Itoh, Y, additional, Kaneko, M, additional, Kawamata, Y, additional, Kawasaki, K, additional, Kikuchi, M, additional, Kimura, T, additional, Kishimoto, H, additional, Kitahara, K, additional, Kitsunezaki, A, additional, Kodama, K, additional, Koide, Y, additional, Koike, T, additional, Kondo, I, additional, Konoshima, S, additional, Kubo, H, additional, Kunieda, S, additional, Kurihara, K, additional, Kuroda, T, additional, Maeda, H, additional, Maeno, M, additional, Matsukawa, M, additional, Matsukawa, T, additional, Matsuo, M, additional, Miya, N, additional, Miyachi, K, additional, Miyo, Y, additional, Mizuno, M, additional, Murakami, Y, additional, Mutoh, M, additional, Nagami, M, additional, Nagashima, A, additional, Nagashima, K, additional, Nagaya, S, additional, Nakamura, H, additional, Nakamura, Y, additional, Nemoto, M, additional, Neyatani, Y, additional, Niikura, S, additional, Ninomiya, H, additional, Nishitani, T, additional, Nishiyama, T, additional, Nomata, H, additional, Noshiroya, S, additional, Ogiwara, N, additional, Ohasa, K, additional, Ohkubo, M, additional, Ohmori, K, additional, Ohmori, S, additional, Ohmori, Y, additional, Ohsato, Y, additional, Ohshima, T, additional, Ohta, M, additional, Otsu, K, additional, Oikawa, A, additional, Ozeki, T, additional, Sakasai, A, additional, Sakata, S, additional, Sato, M, additional, Seimiya, M, additional, Seki, S, additional, Shiho, M, additional, Shitomi, M, additional, Shimada, R, additional, Shimizu, K, additional, Shimizu, M, additional, Shimomura, Y, additional, Shinozaki, S, additional, Shirai, H, additional, Sugie, T, additional, Sunaoshi, H, additional, Suzuki, K, additional, Suzuki, M, additional, Suzuki, S, additional, Suzuki, Y, additional, Tahira, S, additional, Takahashi, M, additional, Takahashi, S, additional, Takahashi, T, additional, Takatsu, H, additional, Takayasu, Y, additional, Takeda, S, additional, Takeuchi, H, additional, Takizuka, T, additional, Tamura, S, additional, Tanaka, E, additional, Tanaka, T, additional, Tani, K, additional, Terakado, T, additional, Tobita, K, additional, Tokutake, T, additional, Totsuka, T, additional, Toyoshima, N, additional, Tsugita, T, additional, Tsuji, S, additional, Tsukahara, Y, additional, Tsuneoka, M, additional, Ujiie, K, additional, Urakawa, H, additional, Uramoto, Y, additional, Ushigusa, K, additional, Yagyu, J, additional, Yamada, K, additional, Yamamoto, M, additional, Yamashita, O, additional, Yamashita, Y, additional, Yano, K, additional, Yokomizo, H, additional, Yonekawa, I, additional, Yoshida, H, additional, Yoshikawa, M, additional, and Yoshino, R, additional
- Published
- 1986
- Full Text
- View/download PDF
20. Characteristics of the JT-60 divertor and limiter plasmas with high power auxiliary heating
- Author
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Aikawa, H, primary, Akaoka, N, additional, Akasaka, H, additional, Akino, N, additional, Akiyama, T, additional, Ando, T, additional, Annoh, K, additional, Aoyagi, T, additional, Arai, T, additional, Arakawa, K, additional, Araki, M, additional, Azumi, M, additional, Chiba, S, additional, Dairaku, M, additional, Ebisawa, N, additional, Fujii, T, additional, Fukuda, T, additional, Funahashi, A, additional, Furukawa, H, additional, Hamamatsu, K, additional, Hanada, M, additional, Hara, M, additional, Haraguchi, K, additional, Hiratsuka, H, additional, Hirayama, T, additional, Hiroki, S, additional, Hiruta, K, additional, Honda, M, additional, Horiike, H, additional, Hosoda, R, additional, Hosogane, N, additional, Iijima, T, additional, Ikeda, K, additional, Ikeda, Y, additional, Imai, T, additional, Inoue, T, additional, Isaji, N, additional, Isaka, M, additional, Ishida, S, additional, Itami, K, additional, Ichige, N, additional, Itoh, T, additional, Kakizaki, T, additional, Kaminaga, A, additional, Katoh, T, additional, Kawai, M, additional, Kawabe, M, additional, Kawamata, Y, additional, Kawasaki, K, additional, Kikuchi, K, additional, Kikuchi, M, additional, Kimura, H, additional, Kimura, T, additional, Kishimoto, H, additional, Kitamura, S, additional, Kitsunezaki, A, additional, Kiyono, K, additional, Kobayashi, N, additional, Kodama, K, additional, Koide, S, additional, Koide, Y, additional, Koike, T, additional, Komata, M, additional, Kondo, I, additional, Konoshima, S, additional, Kubo, H, additional, Kunieda, S, additional, Kurihara, K, additional, Kuriyama, M, additional, Kuroda, T, additional, Kusaka, M, additional, Kusama, Y, additional, Mabuchi, Y, additional, Maehara, S, additional, Maeno, K, additional, Matoba, T, additional, Matsuda, S, additional, Matsukawa, M, additional, Matsukawa, T, additional, Matsuoka, M, additional, Miura, Y, additional, Miya, N, additional, Miyachi, K, additional, Miyo, Y, additional, Mizuno, M, additional, Mori, M, additional, Moriyama, S, additional, Mutoh, M, additional, Nagami, M, additional, Nagashima, A, additional, Nagashima, K, additional, Nagashima, T, additional, Nagaya, S, additional, Naito, O, additional, Nakamura, H, additional, Nakamura, Y, additional, Nemoto, M, additional, Neyatani, Y, additional, Ninomiya, H, additional, Nishino, N, additional, Nishitani, T, additional, Obara, K, additional, Obinata, H, additional, Ogawa, Y, additional, Ogiwara, N, additional, Ohga, T, additional, Ohara, Y, additional, Ohasa, K, additional, Ohara, H, additional, Ohshima, T, additional, Ohkubo, M, additional, Ohsawa, S, additional, Ohta, K, additional, Ohta, M, additional, Ohtaka, M, additional, Ohuchi, Y, additional, Oikawa, A, additional, Okumura, H, additional, Okumura, Y, additional, Omori, K, additional, Omori, S, additional, Omori, Y, additional, Ozeki, T, additional, Saegusa, M, additional, Saitoh, N, additional, Sakamoto, K, additional, Sakasai, A, additional, Sakata, S, additional, Sasajima, T, additional, Satou, K, additional, Satou, M, additional, Sakurai, A, additional, Sawahata, M, additional, Sebata, T, additional, Seimiya, M, additional, Seki, M, additional, Seki, S, additional, Shibanuma, K, additional, Shimada, R, additional, Shimada, T, additional, Shimizu, K, additional, Shimizu, M, additional, Shimomura, Y, additional, Shinozaki, S, additional, Shirai, H, additional, Shirakata, H, additional, Shitomi, M, additional, Suganuma, K, additional, Sugie, T, additional, Sugiyama, T, additional, Sunaoshi, H, additional, Suzuki, K, additional, Suzuki, M, additional, Suzuki, N, additional, Suzuki, S, additional, Suzuki, Y, additional, Takahashi, M, additional, Takahashi, S, additional, Takahashi, T, additional, Takasaki, M, additional, Takatsu, H, additional, Takeuchi, H, additional, Takeshita, A, additional, Takizuka, T, additional, Tamura, S, additional, Tanaka, S, additional, Tani, K, additional, Terakado, M, additional, Terakado, T, additional, Tobita, K, additional, Tokutake, T, additional, Totsuka, T, additional, Toyoshima, N, additional, Tsuda, F, additional, Tsugita, T, additional, Tsuji, S, additional, Tsukahara, Y, additional, Tsuneoka, M, additional, Uehara, K, additional, Umehara, M, additional, Uramoto, Y, additional, Usami, H, additional, Ushigusa, K, additional, Usui, K, additional, Yagyu, J, additional, Yamagiwa, M, additional, Yamamoto, M, additional, Yamamoto, T, additional, Yamashita, O, additional, Yamazaki, T, additional, Yasukawa, T, additional, Yokokura, K, additional, Yokomizo, H, additional, Yokoyama, K, additional, Yoshikawa, K, additional, Yoshikawa, M, additional, Yoshida, H, additional, Yoshino, R, additional, Yoshioka, Y, additional, Yonekawa, I, additional, Yoneda, T, additional, Watanabe, K, additional, Bell, M.G, additional, Bickerton, R.J, additional, Engelhardt, W, additional, Goldston, R.J, additional, Ilne, E.K, additional, Kaline, J, additional, Kugel, H.W, additional, Mondino, P.L, additional, Soldner, F.X, additional, Takase, Y, additional, Thomas, P.R, additional, and Wong, K.L, additional
- Published
- 1989
- Full Text
- View/download PDF
21. Responses of a flexible non-uniform circular beam in a bounded, viscous fluid
- Author
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Hosoda, R., primary, Price, W.G., additional, and Randall, R., additional
- Published
- 1989
- Full Text
- View/download PDF
22. High power heating results on JT-60
- Author
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Akiba, M, primary, Aikawa, H, additional, Akaoka, N, additional, Akasaka, H, additional, Akino, N, additional, Akiyama, T, additional, Ando, T, additional, Annoh, K, additional, Aoyagi, T, additional, Arai, T, additional, Arakawa, K, additional, Araki, M, additional, Azumi, M, additional, Chiba, S, additional, Dairaku, M, additional, Ebisawa, N, additional, Fujii, T, additional, Fukuda, T, additional, Funahashi, A, additional, Furukawa, H, additional, Hamamatsu, K, additional, Hanada, M, additional, Hara, M, additional, Haraguchi, K, additional, Hiratsuka, H, additional, Hirayama, T, additional, Hiroki, S, additional, Hiruta, K, additional, Honda, M, additional, Horiike, H, additional, Hosoda, R, additional, Hosogane, N, additional, Iijima, T, additional, Ikeda, Y, additional, Ikeda, K, additional, Imai, T, additional, Inoue, T, additional, Isaji, N, additional, Isaka, M, additional, Ishida, S, additional, Itami, K, additional, Ichige, N, additional, Itoh, T, additional, Kakizaki, T, additional, Kaminaga, A, additional, Katoh, T, additional, Kawai, M, additional, Kawabe, M, additional, Kawamata, Y, additional, Kawasaki, K, additional, Kikuchi, K, additional, Kikuchi, M, additional, Kimura, H, additional, Kimura, T, additional, Kishimoto, H, additional, Kitamura, S, additional, Kitsunezaki, A, additional, Kiyono, K, additional, Kobayashi, N, additional, Kodama, K, additional, Koide, S, additional, Koide, Y, additional, Kioke, T, additional, Komata, M, additional, Kondo, I, additional, Konoshima, S, additional, Kubo, H, additional, Kunieda, S, additional, Kurihara, K, additional, Kuriyama, M, additional, Kuroda, T, additional, Kusaka, M, additional, Kusama, Y, additional, Mabuchi, Y, additional, Maehara, S, additional, Maeno, K, additional, Matoba, T, additional, Matsuda, S, additional, Matsukawa, M, additional, Matsukawa, T, additional, Matsuoka, M, additional, Miura, Y, additional, Miya, N, additional, Miyachi, K, additional, Mori, Y, additional, Moriyama, S, additional, Mutoh, M, additional, Nagami, M, additional, Nagashima, A, additional, Nagashima, K, additional, Nagashima, T, additional, Nagaya, S, additional, Naitoh, O, additional, Nakamura, H, additional, Nakamura, Y, additional, Nemoto, M, additional, Neyatani, Y, additional, Ninomiya, H, additional, Nishino, N, additional, Nishitani, T, additional, Obara, K, additional, Obinata, H, additional, Ogawa, Y, additional, Ogiwara, N, additional, Ohga, T, additional, Ohara, Y, additional, Ohasa, K, additional, Oohara, H, additional, Ohshima, T, additional, Ohkubo, M, additional, Ohsawa, S, additional, Ohta, K, additional, Ohta, M, additional, Ohtaka, M, additional, Ohuchi, Y, additional, Oikawa, A, additional, Okumura, H, additional, Okumura, Y, additional, Omori, K, additional, Omori, S, additional, Omori, Y, additional, Ozeki, T, additional, Saegusa, M, additional, Saitoh, N, additional, Sakamoto, K, additional, Sakasai, A, additional, Sakata, S, additional, Sasajima, T, additional, Satou, K, additional, Satou, M, additional, Sakurai, A, additional, Sawahata, M, additional, Sebata, T, additional, Seimiya, M, additional, Seki, M, additional, Seki, S, additional, Shibanuma, K, additional, Shimada, R, additional, Shimada, T, additional, Shimizu, K, additional, Shimizu, M, additional, Shimomura, Y, additional, Shinozaki, S, additional, Shirai, H, additional, Shirakata, H, additional, Shitomi, M, additional, Suganuma, K, additional, Sugie, T, additional, Sugiyama, T, additional, Sunaoshi, H, additional, Suzuki, K, additional, Suzuki, M, additional, Suzuki, N, additional, Suzuki, S, additional, Suzuki, Y, additional, Takahashi, M, additional, Takahashi, S, additional, Takahashi, T, additional, Takasaki, M, additional, Takatsu, H, additional, Takeuchi, H, additional, Takeshita, A, additional, Takizuka, T, additional, Tamura, S, additional, Tanaka, S, additional, Tanaka, T, additional, Tani, K, additional, Terakado, M, additional, Terakado, T, additional, Tobita, K, additional, Tokutake, T, additional, Totsuka, T, additional, Toyoshima, N, additional, Tusda, F, additional, Tsugita, T, additional, Tsuji, S, additional, Tsukahara, Y, additional, Tsuneoka, M, additional, Uehara, K, additional, Umechara, M, additional, Uramoto, Y, additional, Usami, H, additional, Ushigusa, K, additional, Usui, K, additional, Yagyu, J, additional, Yamagiwa, M, additional, Yamamoto, M, additional, Yamamoto, T, additional, Yamashita, O, additional, Yamazaki, T, additional, Yasukawa, T, additional, Yokokura, K, additional, Yokomizo, H, additional, Yokoyama, K, additional, Yoshikawa, K, additional, Yoshikawa, M, additional, Yoshida, H, additional, Yoshino, R, additional, Yoshioka, Y, additional, Yonekawa, I, additional, Yoneda, T, additional, Watanabe, K, additional, Bell, M G, additional, Bickerton, R J, additional, Englehardt, W, additional, Goldston, R J, additional, Kallne, E, additional, Kallne, J, additional, Jugel, H W, additional, Mondiono, P L, additional, Solnder, F X, additional, Takase, Y, additional, Thomas, P R, additional, and Wong, K L, additional
- Published
- 1988
- Full Text
- View/download PDF
23. JT-60 experiments
- Author
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Abe, T., primary, Aikawa, H., additional, Akaoka, N., additional, Akasaka, H., additional, Akiba, M., additional, akino, N., additional, Akiyama, T., additional, Ando, T., additional, Anno, K., additional, Aoyagi, T., additional, Arai, T., additional, Arakawa, K., additional, Araki, M., additional, Azumi, M., additional, Dairaku, M., additional, Ebisawa, N., additional, Fujii, T., additional, Fukuda, T., additional, Furukawa, H., additional, Hamamatsu, K., additional, Haraguchi, T., additional, Hayashi, K., additional, Hiratsuka, H., additional, Hirayama, T., additional, lida, K., additional, Hiroki, S., additional, Hiruta, K., additional, Hitomi, N., additional, Honda, M., additional, Horiike, H., additional, Hosoda, R., additional, Hosogane, N., additional, Ichige, H., additional, Iida, S., additional, Iijima, T., additional, Ikeda, Y., additional, Imai, T., additional, Inami, H., additional, Aisaji, N., additional, Isaka, M., additional, Ishihara, M., additional, Itoh, H., additional, Itoh, Y., additional, Kanai, T., additional, Katoh, T., additional, Kawai, M., additional, Kawamata, Y., additional, Kihara, Y., additional, Kawasaki, K., additional, Kikuchi, M., additional, Kimura, H., additional, Kimura, T., additional, Kishimoto, H., additional, Kitahara, K., additional, Kitamura, S., additional, Kutsunezaki, A., additional, Kiyono, K., additional, Kodama, K., additional, Koide, Y., additional, Koike, T., additional, Komata, M., additional, Kondo, I., additional, Konoshima, S., additional, Kubo, H., additional, Kunieda, S., additional, Kurakada, S., additional, Kurihara, K., additional, Kuriyama, M., additional, Kuroda, T., additional, Maeno, M., additional, Matsuda, S., additional, Matsukawa, M., additional, Matsukawa, T., additional, Matsuo, M., additional, Matsuoka, M., additional, Miya, N., additional, Miyachi, K., additional, Miyo, Y., additional, Mizuhashi, K., additional, Mizuno, M., additional, Murakami, Y., additional, Mutoh, M., additional, Nagami, M., additional, Nagashima, A., additional, Nagashima, K., additional, Nagaya, S., additional, Nakamura, H., additional, Nakamura, Y., additional, Nagashima, T., additional, Nemoto, M., additional, Neyatani, Y., additional, Niikura, S., additional, Ninomiya, H., additional, Nishitani, T., additional, Nishiyama, T., additional, Nomata, H., additional, Noshiroya, S., additional, Ogiwara, N., additional, Ohasa, K., additional, Ohga, T., additional, Ohhara, H., additional, Ohkubo, M., additional, Ohmori, K., additional, ohmori, S., additional, Ohmori, Y., additional, ohsato, Y., additional, Ohshima, T., additional, Ohta, M., additional, Ohara, Y., additional, Ohuchi, Y., additional, Okumura, Y., additional, Otsu, K., additional, Oikawa, A., additional, Ozeki, T., additional, Saigusa, M., additional, Sakamoto, K., additional, Sakasai, A., additional, Sakata, S., additional, Sato, M., additional, Sawahata, M., additional, Shibanuma, K., additional, Shibata, T., additional, Seimiya, M., additional, Seki, M., additional, Seki, S., additional, Shitomi, M., additional, Shimada, R., additional, Shimizu, K., additional, Shimizu, M., additional, Shimomura, Y., additional, Shinozaki, S., additional, Shirai, H., additional, Shirakata, H., additional, Suganuma, K., additional, Sugawara, T., additional, Sugie, T., additional, Sunaoshi, H., additional, Suzuki, K., additional, Suzuki, M., additional, Suzuki, N., additional, Suzuki, S., additional, Suzuki, Y., additional, Tahira, S., additional, Takahashi, M., additional, Takahashi, S., additional, Takahashi, T., additional, Takatsu, H., additional, Takayasu, Y., additional, Takeda, S., additional, Takeuchi, H., additional, Takizua, T., additional, Tamura, S., additional, Tanaka, E., additional, Tanaka, S., additional, Tanaka, T., additional, Tani, K., additional, Terakado, T., additional, Tobita, K., additional, Tokutake, T., additional, Totsuka, T., additional, Toyoshima, N., additional, Tsugita, T., additional, Tsuji, S., additional, Tsukahara, Y., additional, Tsuneoka, M., additional, Uehara, K., additional, Uehara, M., additional, Ujiie, K., additional, Urakawa, H., additional, Uramoto, Y., additional, Ushigusa, K., additional, Usui, K., additional, Watanabe, K., additional, Yagyu, J., additional, Yamada, K., additional, Yamamoto, M., additional, Yamashita, O., additional, Yamashita, Y., additional, Yano, K., additional, Yokokura, K., additional, Yokomizo, H., additional, Yonekawa, I., additional, Yoshida, H., additional, Yoshikawa, M., additional, and Yoshino, R., additional
- Published
- 1987
- Full Text
- View/download PDF
24. Application of marine radar for ocean wave monitoring
- Author
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Kojima, T., primary, Hosoda, R., additional, Susaki, H., additional, Hiraoka, Y., additional, Iwasaki, Y., additional, and Yamada, T., additional
- Full Text
- View/download PDF
25. Elimination of environmental impact loads from processed efflux
- Author
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Hosoda, R., primary, Murota, S., additional, Hamamiya, D., additional, Yao, M., additional, Fujinami, H., additional, and Hata, N., additional
- Full Text
- View/download PDF
26. Concept of Eco-Platform
- Author
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Hosoda, R., primary, Nomura, T., additional, Yamaguchi, K., additional, and Murata, B., additional
- Full Text
- View/download PDF
27. Analyses of facial expressions for the evaluation of seasickness
- Author
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Arima, M., primary, Ikeda, K., additional, and Hosoda, R., additional
- Full Text
- View/download PDF
28. Assmilation model of marine environmental data measured by different organizations in Osaka Bay
- Author
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Yamada, T., primary and Hosoda, R., additional
- Full Text
- View/download PDF
29. Analyses of facial expressions for the evaluation of seasickness.
- Author
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Arima, M., Ikeda, K., and Hosoda, R.
- Published
- 2004
- Full Text
- View/download PDF
30. Elimination of environmental impact loads from processed efflux.
- Author
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Hosoda, R., Murota, S., Hamamiya, D., Yao, M., Fujinami, H., and Hata, N.
- Published
- 2004
- Full Text
- View/download PDF
31. Assimilation model of marine environmental data measured by different organizations in Osaka Bay.
- Author
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Yamada, T. and Hosoda, R.
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- 2004
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32. Concept of Eco-Platform.
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Hosoda, R., Nomura, T., Yamaguchi, K., and Murata, B.
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- 2000
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33. Characteristics of the JT-60 divertor and limiter plasmas with high power auxiliary heating
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The JT-60 Team, Aikawa, H, Akaoka, N, Akasaka, H, Akino, N, Akiyama, T, Ando, T, Annoh, K, Aoyagi, T, Arai, T, Arakawa, K, Araki, M, Azumi, M, Chiba, S, Dairaku, M, Ebisawa, N, Fujii, T, Fukuda, T, Funahashi, A, Furukawa, H, Hamamatsu, K, Hanada, M, Hara, M, Haraguchi, K, Hiratsuka, H, Hirayama, T, Hiroki, S, Hiruta, K, Honda, M, Horiike, H, Hosoda, R, Hosogane, N, Iijima, T, Ikeda, K, Ikeda, Y, Imai, T, Inoue, T, Isaji, N, Isaka, M, Ishida, S, Itami, K, Ichige, N, Itoh, T, Kakizaki, T, Kaminaga, A, Katoh, T, Kawai, M, Kawabe, M, Kawamata, Y, Kawasaki, K, Kikuchi, K, Kikuchi, M, Kimura, H, Kimura, T, Kishimoto, H, Kitamura, S, Kitsunezaki, A, Kiyono, K, Kobayashi, N, Kodama, K, Koide, S, Koide, Y, Koike, T, Komata, M, Kondo, I, Konoshima, S, Kubo, H, Kunieda, S, Kurihara, K, Kuriyama, M, Kuroda, T, Kusaka, M, Kusama, Y, Mabuchi, Y, Maehara, S, Maeno, K, Matoba, T, Matsuda, S, Matsukawa, M, Matsukawa, T, Matsuoka, M, Miura, Y, Miya, N, Miyachi, K, Miyo, Y, Mizuno, M, Mori, M, Moriyama, S, Mutoh, M, Nagami, M, Nagashima, A, Nagashima, K, Nagashima, T, Nagaya, S, Naito, O, Nakamura, H, Nakamura, Y, Nemoto, M, Neyatani, Y, Ninomiya, H, Nishino, N, Nishitani, T, Obara, K, Obinata, H, Ogawa, Y, Ogiwara, N, Ohga, T, Ohara, Y, Ohasa, K, Ohara, H, Ohshima, T, Ohkubo, M, Ohsawa, S, Ohta, K, Ohta, M, Ohtaka, M, Ohuchi, Y, Oikawa, A, Okumura, H, Okumura, Y, Omori, K, Omori, S, Omori, Y, Ozeki, T, Saegusa, M, Saitoh, N, Sakamoto, K, Sakasai, A, Sakata, S, Sasajima, T, Satou, K, Satou, M, Sakurai, A, Sawahata, M, Sebata, T, Seimiya, M, Seki, M, Seki, S, Shibanuma, K, Shimada, R, Shimada, T, Shimizu, K, Shimizu, M, Shimomura, Y, Shinozaki, S, Shirai, H, Shirakata, H, Shitomi, M, Suganuma, K, Sugie, T, Sugiyama, T, Sunaoshi, H, Suzuki, K, Suzuki, M, Suzuki, N, Suzuki, S, Suzuki, Y, Takahashi, M, Takahashi, S, Takahashi, T, Takasaki, M, Takatsu, H, Takeuchi, H, Takeshita, A, Takizuka, T, Tamura, S, Tanaka, S, Tani, K, Terakado, M, Terakado, T, Tobita, K, Tokutake, T, Totsuka, T, Toyoshima, N, Tsuda, F, Tsugita, T, Tsuji, S, Tsukahara, Y, Tsuneoka, M, Uehara, K, Umehara, M, Uramoto, Y, Usami, H, Ushigusa, K, Usui, K, Yagyu, J, Yamagiwa, M, Yamamoto, M, Yamamoto, T, Yamashita, O, Yamazaki, T, Yasukawa, T, Yokokura, K, Yokomizo, H, Yokoyama, K, Yoshikawa, K, Yoshikawa, M, Yoshida, H, Yoshino, R, Yoshioka, Y, Yonekawa, I, Yoneda, T, Watanabe, K, Bell, M.G, Bickerton, R.J, Engelhardt, W, Goldston, R.J, Ilne, E.K, Kaline, J, Kugel, H.W, Mondino, P.L, Soldner, F.X, Takase, Y, Thomas, P.R, and Wong, K.L
- Published
- 1989
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34. Effect of (S)- α-fluoromethylhistidine and SMS 201-995 on growth of histamine-producing gastric carcinoid of Mastomys natalensis.
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Hosoda, S., Hosoda, R., and Konishi, T.
- Abstract
(S)- α-fluoromethylhistidine (FMH) administered by either i.p. injection or continuous s.c. infusion leads to marked depletion of histamine levels in urine of Mastomys bearing transplantable histamine-producing argyrophilic gastric carcinoid. The continuous s.c. injection of FMH appears to be superior to i.p. injection for suppressing HDC activity in carcinoid cells. FMH administered by either route, however, does not appear to retard the growth of this tumour strain. Among 10 Mastomys receiving daily s.c. injection of SMS (500 μg/kg), only 2 Mastomys's tumours regressed during treatment, but after termination of SMS, they grew again. In the s.c. infusion groups administered with 200 and 400 μg of SMS, a definite effect of SMS on the tumour growth could not be obtained. [ABSTRACT FROM AUTHOR]
- Published
- 1993
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35. Disappearance of a chromosomal abnormality in a young patient with polycythemia vera treated with ropeginterferon alfa-2b.
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Hosoda R, Hashimoto Y, Hara K, Maegaki M, Suzuki S, Hosoda Y, and Kawamura K
- Published
- 2024
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36. Transcriptional dysregulation of autophagy in the muscle of a mouse model of Duchenne muscular dystrophy.
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Nakashima R, Hosoda R, Tatekoshi Y, Iwahara N, Saga Y, and Kuno A
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- Humans, Animals, Mice, Dystrophin genetics, Mice, Inbred mdx, Muscle, Skeletal metabolism, Autophagy genetics, Disease Models, Animal, Muscular Dystrophy, Duchenne genetics, Muscular Dystrophy, Duchenne metabolism
- Abstract
It has been reported that autophagic activity is disturbed in the skeletal muscles of dystrophin-deficient mdx mice and patients with Duchenne muscular dystrophy (DMD). Transcriptional regulations of autophagy by FoxO transcription factors (FoxOs) and transcription factor EB (TFEB) play critical roles in adaptation to cellular stress conditions. Here, we investigated whether autophagic activity is dysregulated at the transcription level in dystrophin-deficient muscles. Expression levels of autophagy-related genes were globally decreased in tibialis anterior and soleus muscles of mdx mice compared with those of wild-type mice. DNA microarray data from the NCBI database also showed that genes related to autophagy were globally downregulated in muscles from patients with DMD. These downregulated genes are known as targets of FoxOs and TFEB. Immunostaining showed that nuclear localization of FoxO1 and FoxO3a was decreased in mdx mice. Western blot analyses demonstrated increases in phosphorylation levels of FoxO1 and FoxO3a in mdx mice. Nuclear localization of TFEB was also reduced in mdx mice, which was associated with elevated phosphorylation levels of TFEB. Collectively, the results suggest that autophagy is disturbed in dystrophin-deficient muscles via transcriptional downregulation due to phosphorylation-mediated suppression of FoxOs and TFEB., (© 2024. The Author(s).)
- Published
- 2024
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37. Resveratrol, a SIRT1 activator, attenuates aging-associated alterations in skeletal muscle and heart in mice.
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Hosoda R, Nakashima R, Yano M, Iwahara N, Asakura S, Nojima I, Saga Y, Kunimoto R, Horio Y, and Kuno A
- Subjects
- Mice, Animals, Resveratrol pharmacology, Sirtuin 1 metabolism, Muscle, Skeletal metabolism, Aging, Myocytes, Cardiac metabolism, Hypertrophy, Sarcopenia drug therapy, Sarcopenia metabolism, Stilbenes pharmacology, Stilbenes therapeutic use
- Abstract
Aging is associated with impairment of multiple organs, including skeletal muscle and heart. In this study, we investigated whether resveratrol, an activator of an NAD
+ -dependent protein deacetylase Sirtuin-1 (SIRT1), attenuates age-related sarcopenia and cardiomyocyte hypertrophy in mice. Treatment of mice with resveratrol (0.4 g/kg diet) from 28 weeks of age for 32 weeks prevented aging-associated shortening of rotarod riding time. In the tibialis anterior (TA) muscle, histogram analysis showed that the atrophic muscle was increased in 60-week-old (wo) mice compared with 20-wo mice, which was attenuated by resveratrol. In the heart, resveratrol attenuated an aging-associated increase in the cardiomyocyte diameter. Acetylated proteins were increased and autophagic activity was reduced in the TA muscle of 60-wo mice compared with those of 20-wo mice. Resveratrol treatment reduced levels of acetylated proteins and restored autophagic activity in the TA muscle. Aging-related reduction in myocardial autophagy was also suppressed by resveratrol. Skeletal muscle-specific SIRT1 knockout mice showed increases in acetylated proteins and atrophic muscle fibers and reduced autophagic activity in the TA muscle. These results suggest that activation of SIRT1 by treatment with resveratrol suppresses sarcopenia and cardiomyocyte hypertrophy by restoration of autophagy in mice., Competing Interests: Competing interests The authors declare no competing interests., (Copyright © 2023 The Authors. Production and hosting by Elsevier B.V. All rights reserved.)- Published
- 2023
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38. Development of a Prognostic Scoring System using MYC Expression and Soluble Interleukin Receptor -2 level for Diffuse Large B-cell Lymphoma.
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Suzuki S, Kuwamoto S, Kawamura K, Matsushita M, Motokura T, Hosoda Y, Maegaki M, Hosoda R, Hara K, Umekita Y, and Fukuda T
- Abstract
Background: Diffuse large B-cell lymphoma, not otherwise specified (DLBCL-NOS), is the most frequent type of lymphoid neoplasm., Methods: We investigated the relationships between clinical factors of DLBCL-NOS and MYC immunohistochemistry (IHC) staining., Results: A total of 110 patients diagnosed with DLBCL-NOS from 2012 to 2020 at Tottori University Hospital and treated with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisolone (R-CHOP) chemotherapy were included. IHC staining of MYC in formalin-fixed, paraffin-embedded tumor specimens was performed, and ROC-curve analysis revealed the cut-off value of the MYC positive rate as 55%. The 2-year overall survival (OS) rates of the MYC-negative and -positive groups were 84.7% vs 57.7% ( P = 0.0091), and the progression-free survival rates were 77.8% vs 54.7% ( P = 0.016), respectively. Multivariate analysis for OS showed prognostic significance of MYC positivity [hazards ratio (HR): 2.496; P = 0.032], and serum levels of soluble interleukin-2 receptor (sIL-2R) > 2000 U/mL (HR: 3.950; P = 0.0019), as well as age > 75 (HR: 2.356; P = 0.068). The original scoring system was developed based on these findings. By assigning one point to each item, age (> 75), MYC positivity, and sIL-2R level (> 2000), all patients were classified into three risk categories: group 1 (0 points), group 2 (1 point), and group 3 (2-3 points). The 2-year survival rates were 100%, 83.0%, and 47.1% for the groups 1, 2, and 3, respectively ( P < 0.0001)., Conclusion: We suggest that a prognostic scoring system using MYC expression and soluble interleukin receptor -2 level is useful for the prediction of prognosis, contributing to further stratification in DLBCL-NOS., Competing Interests: The authors declare no conflict of interest., (©2023 Tottori University Medical Press.)
- Published
- 2023
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39. SIRT1 in the cardiomyocyte counteracts doxorubicin-induced cardiotoxicity via regulating histone H2AX.
- Author
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Kuno A, Hosoda R, Tsukamoto M, Sato T, Sakuragi H, Ajima N, Saga Y, Tada K, Taniguchi Y, Iwahara N, and Horio Y
- Subjects
- Mice, Animals, Cardiotoxicity metabolism, Caspase 3 metabolism, Sirtuin 1 genetics, Sirtuin 1 metabolism, Doxorubicin toxicity, Apoptosis, Histones metabolism, Myocytes, Cardiac metabolism
- Abstract
Aims: Cardiotoxicity by doxorubicin predicts worse prognosis of patients. Accumulation of damaged DNA has been implicated in doxorubicin-induced cardiotoxicity. SIRT1, an NAD+-dependent histone/protein deacetylase, protects cells by deacetylating target proteins. We investigated whether SIRT1 counteracts doxorubicin-induced cardiotoxicity by mediating Ser139 phosphorylation of histone H2AX, a critical signal of the DNA damage response., Methods and Results: Doxorubicin (5 mg/kg per week, x4) was administered to mice with intact SIRT1 (Sirt1f/f) and mice that lack SIRT1 activity in cardiomyocytes (Sirt1f/f;MHCcre/+). Reductions in left ventricular fractional shortening and ejection fraction by doxorubicin treatment were more severe in Sirt1f/f;MHCcre/+ than in Sirt1f/f. Myocardial expression level of type-B natriuretic peptide was 2.5-fold higher in Sirt1f/f;MHCcre/+ than in Sirt1f/f after doxorubicin treatment. Sirt1f/f;MHCcre/+ showed larger fibrotic areas and higher nitrotyrosine levels in the heart after doxorubicin treatment. Although doxorubicin-induced DNA damage evaluated by TUNEL staining was enhanced in Sirt1f/f;MHCcre/+, the myocardium from Sirt1f/f;MHCcre/+ showed blunted Ser139 phosphorylation of H2AX by doxorubicin treatment. In H9c2 cardiomyocytes, SIRT1 knockdown attenuated Ser139 phosphorylation of H2AX, increased DNA damage, and enhanced caspase-3 activation under doxorubicin treatment. Immunostaining revealed that acetylation level of H2AX at Lys5 was higher in hearts from Sirt1f/f;MHCcre/+. In H9c2 cells, acetyl-Lys5-H2AX level was increased by SIRT1 knockdown and reduced by SIRT1 overexpression. Ser139 phosphorylation in response to doxorubicin treatment was blunted in a mutant H2AX with substitution of Lys5 to Gln (K5Q) that mimics acetylated lysine compared with that in wild-type H2AX. Expression of K5Q-H2AX as well as S139A-H2AX, which cannot be phosphorylated at Ser139, augmented doxorubicin-induced caspase-3 activation. Treatment of mice with resveratrol, a SIRT1 activator, attenuated doxorubicin-induced cardiac dysfunction, which was associated with a reduction in acetyl-Lys5-H2AX level and a preserved phospho-Ser139-H2AX level., Conclusion: These findings suggest that SIRT1 counteracts doxorubicin-induced cardiotoxicity by mediating H2AX phosphorylation through its deacetylation in cardiomyocytes., Competing Interests: Conflict of interest none declared., (Published on behalf of the European Society of Cardiology. All rights reserved. © The Author(s) 2022. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2023
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40. [Fatal invasive pneumococcal disease developed 14 years after allogeneic hematopoietic stem cell transplantation in a patient with myelodysplastic syndrome].
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Hosoda R, Kawamura K, Hara K, Maegaki M, Suzuki S, Hosoda Y, Morishita S, Chikumi H, Motokura T, and Fukuda T
- Subjects
- Humans, Female, Middle Aged, Transplantation, Homologous, Graft vs Host Disease prevention & control, Hematopoietic Stem Cell Transplantation adverse effects, Myelodysplastic Syndromes complications, Myelodysplastic Syndromes therapy, Pneumococcal Infections etiology
- Abstract
Invasive pneumococcal diseases (IPDs) after allogeneic hematopoietic stem cell transplantation have high fatality rates and often develop late after transplantation. The patient was a 58-year-old female. Fourteen years ago, she underwent bone marrow transplantation from a HLA-DR 1-antigen mismatched unrelated donor for myelodysplastic syndrome. She developed pneumonia, chronic graft-versus-host disease, and hypogammaglobulinemia. She received 23-valent pneumococcal capsular polysaccharide vaccine 11 and 6 years earlier. She was presented to our emergency room with fever. Her blood culture was positive for pneumococcus, and she was diagnosed with an IPD. The patient received antibiotic treatment but died on the third day of hospitalization. Because of its seriousness, pneumococcal infection should receive attention even 10 or more years after transplantation. Preventive approaches such as vaccination and early intervention at the time of diagnosis are important.
- Published
- 2023
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41. Safety and feasibility of autologous cord blood infusion for improving motor function in young children with cerebral palsy in Japan: A single-center study.
- Author
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Kikuchi H, Saitoh S, Tsuno T, Hosoda R, Baba N, Wang F, Mitsuda N, Tsuda M, Maeda N, Sagara Y, and Fujieda M
- Subjects
- Humans, Fetal Blood, Japan, Feasibility Studies, Pilot Projects, Motor Skills, Cerebral Palsy therapy
- Abstract
Introduction: Cerebral palsy (CP) is the most prevalent motor disorder of childhood. It typically results from in utero or perinatal brain injury. Recently, it has been reported that autologous cord blood (ACB) infusion for children with CP improved gross motor function and brain connectivity, but unfortunately, it has never been tried in Japan. We conducted a pilot study of the infusing of ACB, which was delivered from private bank, in the children with CP to assess the safety and feasibility to the procedure as well as any effect in improving neurological function., Methods: After demonstrating the induction of tissue regeneration in animal model studies conducted a single-arm pilot study of intravenous ACB infusion in 6 young Japanese children with CP (ages 1-6 years). Primary outcomes were safety assessed by vital signs, clinical symptoms, and blood and urinary examinations at baseline and 1 weeks, 1, 2 and 3 years after treatment. In addition, motor function evaluations, neurodevelopmental examinations, magnetic resonance imaging, and electroencephalography (EEG) were performed at the same time., Results: Infusion was generally well-tolerated, although one patient experienced microhematuria 1 year after treatment and another one patient experienced febrile convulsion once 9 months after treatment. These events were transient, no relapse was seen during observation study. All patients improved a median of 6.8 points on the 1-year Gross Motor Functional Measure-66 (GMFM-66) scores, greater than predicted by age and severity. Furthermore, the 2-year and 3-year GMFM-66 scores were also greater than expected (median 6.2 points and 5.5 points, respectively). Overall scales and language-social scales of the developmental quotient (DQ) improved in 3 of 6 patients, who had greater changes in their GMFM-66 scores than the other cases after treatment. There were no significant correlations among the GMFM-66 scores, DQ, and infusion cell counts., Conclusion: ACB infusion was safe and feasible for clinical use in patients with CP. However, much more clinical study with larger numbers of patients and in-depth studies of treatment mechanism of CP are needed., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Japanese Society of Child Neurology. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
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42. Successful HLA-haploidentical stem cell transplantation with post-transplant cyclophosphamide in an older patient with chronic active Epstein-Barr virus infection.
- Author
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Maegaki M, Kawamura K, Hara K, Hosoda R, Suzuki S, Hosoda Y, Imadome KI, Motokura T, and Fukuda T
- Subjects
- Cyclophosphamide therapeutic use, Etoposide therapeutic use, Female, HLA Antigens, Herpesvirus 4, Human, Humans, Melphalan therapeutic use, Middle Aged, Prednisolone therapeutic use, Transplantation Conditioning adverse effects, Cyclosporins therapeutic use, Epstein-Barr Virus Infections complications, Graft vs Host Disease etiology, Hematopoietic Stem Cell Transplantation adverse effects
- Abstract
Chronic active Epstein-Barr virus infection (CAEBV) is a subtype of EBV-associated T/NK cell lymphoproliferative disease and is only curable by allogeneic hematopoietic stem cell transplantation. However, finding a human leukocyte antigen (HLA)-matched donor at a suitable time can sometimes be difficult. We report the case of a 60-year-old woman who received prednisolone (PSL) after being diagnosed with autoimmune hepatitis 3 years earlier. She suddenly developed high fever and impaired liver function. Based on a high EBV DNA load in the peripheral blood, CAEBV was diagnosed. The patient was started on cooling therapy with PSL, cyclosporine, and etoposide, which reduced symptoms. Subsequently, she received HLA-haploidentical stem cell transplantation (haplo-SCT) with reduced-intensity conditioning (fludarabine 25 mg/m
2 for 5 days, melphalan 50 mg/m2 for 2 days, and total body irradiation at 2 Gy) and post-transplant cyclophosphamide (PTCy) because she lacked an HLA-matched donor. Liver function was restored, and EBV DNA load in peripheral white blood cells became undetectable. The patient is alive without relapse or severe complications over 1 year after transplantation. To our knowledge, this is the first report of successful haplo-SCT with PTCy for CAEBV. This approach may be an alternative therapeutic option for CAEBV patients lacking an HLA-matched donor., (© 2022. Japanese Society of Hematology.)- Published
- 2022
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43. Activation of SIRT1 promotes membrane resealing via cortactin.
- Author
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Iwahara N, Azekami K, Hosoda R, Nojima I, Hisahara S, and Kuno A
- Subjects
- Actins, Animals, Cortactin metabolism, Mice, Mice, Inbred mdx, Resveratrol pharmacology, Muscular Dystrophies, Sirtuin 1 genetics, Sirtuin 1 metabolism
- Abstract
Muscular dystrophies are inherited myopathic disorders characterized by progressive muscle weakness. Recently, several gene therapies have been developed; however, the treatment options are still limited. Resveratrol, an activator of SIRT1, ameliorates muscular function in muscular dystrophy patients and dystrophin-deficient mdx mice, although its mechanism is still not fully elucidated. Here, we investigated the effects of resveratrol on membrane resealing. We found that resveratrol promoted membrane repair in C2C12 cells via the activation of SIRT1. To elucidate the mechanism by which resveratrol promotes membrane resealing, we focused on the reorganization of the cytoskeleton, which occurs in the early phase of membrane repair. Treatment with resveratrol promoted actin accumulation at the injured site. We also examined the role of cortactin in membrane resealing. Cortactin accumulated at the injury site, and cortactin knockdown suppressed membrane resealing and reorganization of the cytoskeleton. Additionally, SIRT1 deacetylated cortactin and promoted the interaction between cortactin and F-actin, thus possibly enhancing the accumulation of cortactin at the injury site. Finally, we performed a membrane repair assay using single fiber myotubes from control and resveratrol-fed mice, where the oral treatment with resveratrol promoted membrane repair ex vivo. These findings suggest that resveratrol promotes membrane repair via the SIRT1/cortactin axis., (© 2022. The Author(s).)
- Published
- 2022
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44. Downregulation of IGFBP5 contributes to replicative senescence via ERK2 activation in mouse embryonic fibroblasts.
- Author
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Nojima I, Hosoda R, Toda Y, Saito Y, Ueda N, Horimoto K, Iwahara N, Horio Y, and Kuno A
- Subjects
- Animals, Cell Proliferation, Down-Regulation, Mice, Phosphorylation, Cellular Senescence genetics, Fibroblasts metabolism
- Abstract
Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are secretory proteins that regulate IGF signaling. In this study, we investigated the role of IGFBP5 in replicative senescence in embryonic mouse fibroblasts (MEFs). During passages according to the 3T3 method, MEFs underwent senescence after the 5th passage (P5) based on cell growth arrest, an increase in the number of cells positive for senescence-associated β-galactosidase (SA-β-GAL) staining, and upregulation of p16 and p19. In P8 MEFs, IGFBP5 mRNA level was markedly reduced compared with that in P2 MEFs. Downregulation of IGFBP5 via siRNA in P2 MEFs increased the number of SA-β-GAL-positive cells, upregulated p16 and p19, and inhibited cell growth. Incubation of MEFs with IGFBP5 during serial passage increased the cumulative population doubling and decreased SA-β-GAL positivity compared with those in vehicle-treated cells. IGFBP5 knockdown in P2 MEFs increased phosphorylation levels of ERK1 and ERK2. Silencing of ERK2, but not that of ERK1, blocked the increase in the number of SA-β-GAL-positive cells in IGFBP5-knockdown cells. The reduction in the cell number and upregulation of p16 and p21 in IGFBP5-knockdown cells were attenuated by ERK2 knockdown. Our results suggest that downregulation of IGFBP5 during serial passage contributes to replicative senescence via ERK2 in MEFs.
- Published
- 2022
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45. Ubiquitin-dependent rapid degradation conceals a cell-protective function of cytoplasmic SIRT3 against oxidative stress.
- Author
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Hayashi T, Matsushita T, Hisahara S, Iwahara N, Kuno A, Kunimoto R, Hosoda R, Tanno M, Shimohama S, and Horio Y
- Subjects
- Animals, Hydrogen Peroxide metabolism, Mice, Mitochondria metabolism, Oxidative Stress, PC12 Cells, Rats, Ubiquitin metabolism, Sirtuin 3 genetics, Sirtuin 3 metabolism
- Abstract
SIRT3 is an NAD+-dependent protein deacetylase localized in mitochondria. Several studies reported localization of SIRT3 in the cytoplasm or nucleus, but data of these studies were not consistent. We detected expression of mitochondrial (SIRT3mt) and cytoplasmic (SIRT3ct) Sirt3 mRNAs in the mouse brain, and we also found SIRT3 immunostaining of mitochondria and cytoplasm in the brain and cultured neural cells. However, expression levels of SIRT3ct in COS cells transfected with SIRT3ct cDNA were much lower than those of SIRT3mt. We found that SIRT3ct but not SIRT3mt was promptly degraded by ubiquitin-dependent degradation, in which SIRT3ct degradation was mediated mainly by ubiquitination of NH2-terminal methionine and partly by that of lysine residues of SIRT3ct. SIRT3ct expression level was significantly enhanced by the treatment of cells with staurosporine or H2O2. H2O2 treatment promoted nuclear translocation of SIRT3ct and induced histone H3 deacetylation and superoxide dismutase 2 expression. Overexpression of SIRT3ct decreased cell death caused by H2O2 at levels similar to those achieved by overexpression of SIRT3mt. Knockdown of Sirt3 mRNA increased cell death caused by amyloid-β (Aβ), and overexpression of SIRT3ct suppressed the toxic function of Aβ in PC12 cells. These results indicate that SIRT3ct promotes cell survival under physiological and pathological conditions., (© The Author(s) 2021. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2022
- Full Text
- View/download PDF
46. Catheter-related bloodstream infection associated with multiple insertions of the peripherally inserted central catheter in patients with hematological disorders.
- Author
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Hashimoto Y, Hosoda R, Omura H, and Tanaka T
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Antineoplastic Combined Chemotherapy Protocols adverse effects, Catheter-Related Infections etiology, Female, Follow-Up Studies, Hematologic Diseases pathology, Humans, Male, Middle Aged, Prognosis, Retrospective Studies, Sepsis etiology, Young Adult, Antineoplastic Combined Chemotherapy Protocols administration & dosage, Catheter-Related Infections pathology, Catheterization, Central Venous adverse effects, Catheterization, Peripheral adverse effects, Central Venous Catheters adverse effects, Hematologic Diseases drug therapy, Sepsis pathology
- Abstract
Patients with hematological disorders are treated with multiple cycles of chemotherapy. As a result, they often require multiple insertions of the peripherally inserted central catheter (PICC) for prolonged periods of time. Although PICCs have been widely used worldwide in various patients, the safety and feasibility of the multiple insertions of the PICC in this population have not been fully verified. We performed a retrospective analysis to clarify the relationship between complications and multiple PICC insertions in patients with hematological disorders who were treated with either chemotherapy or immunotherapy. A total of 651 PICCs were inserted in 261 patients with a median age of 66 years. Acute myeloid leukemia (AML) and non-Hodgkin's lymphoma were the most common diseases in our patient cohort. The total catheter days (CDs) was 29,485 days, with a median catheter duration of 30 days. The rate of catheter-related bloodstream infection (CRBSI) in our patient cohort at high rate of re-insertion was 2.0/1000 CDs. Although multiple PICC insertions were not a risk factor of CRBSI, our findings suggest that a prolonged catheter dwell time may be associated with CRBSI. AML was an important risk factor of CRBSI. While the PICC dwell time depends on the treatment cycle, our findings indicate that it should be limited to approximately 30 days and catheters may be removed and re-inserted as needed.
- Published
- 2021
- Full Text
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47. Successful Percutaneous Thrombectomy of the Left Pulmonary Artery in a Neonate After a Patent Ductus Arteriosus Clipping.
- Author
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Muraji S, Kobayashi T, Oka S, Kojima T, Yoshiba S, Kanai M, Ishido H, Masutani S, Hosoda R, Masuoka A, Suzuki T, and Sumitomo N
- Subjects
- Female, Humans, Infant, Newborn, Ductus Arteriosus, Patent surgery, Postoperative Complications surgery, Pulmonary Embolism surgery, Thrombectomy methods
- Abstract
We report a neonate with a successful percutaneous thrombectomy of a total thrombotic occlusion of the left pulmonary artery (LPA) after a surgical clipping for a patent ductus arteriosus (PDA). We suspected the compression of the LPA by the clipping and postoperative hemodynamic instability caused the LPA obstruction. After the surgical removal of the PDA clip and division of the PDA, we could safely retrieve the LPA thrombus with a non-hydrodynamic thrombectomy catheter for coronary arteries.
- Published
- 2021
- Full Text
- View/download PDF
48. Aging-associated inflammation and fibrosis in arachnoid membrane.
- Author
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Suzuki H, Mikami T, Iwahara N, Akiyama Y, Wanibuchi M, Komatsu K, Yokoyama R, Hirano T, Hosoda R, Horio Y, Kuno A, and Mikuni N
- Subjects
- Adult, Aged, Female, Fibrosis pathology, Humans, Male, Middle Aged, Aging pathology, Arachnoid pathology, Inflammation pathology
- Abstract
Background: The physiological and pathological significance of the arachnoid membrane (AM) is still unknown. In this study, we investigated various characteristics of the AM, focusing on the influence of inflammation and fibrosis., Methods: Small pieces of AM sample were obtained during neurosurgical procedures from 74 cases. The clinical and pathological characteristics of the hyperplastic AM group (≥ 50 μm) and the non-hyperplastic AM group (< 50 μm) were compared. Then, potential correlations between AM thickness and clinical characteristics were analyzed. Moreover, VEGFα, TGFβ, and TGFα levels were quantitated by real time PCR. Then, the potential correlations between AM thickness and these inflammatory or anti-inflammatory markers, and the influence of the original disease were calculated., Results: The median age of the patients in hyperplastic AM group was significantly older than that of the non-hyperplastic AM group. Moreover, the number of fibroblasts, CD68
+ cells, CD86+ cells, and CD206+ cells in the hyperplastic AM group was significantly higher than that in the non-hyperplastic AM group. The AM thickness was significantly correlated to age and number of fibroblasts, CD68+ cells, CD86+ cells, and CD206+ cells. The thickness of the AM was significantly correlated to the messenger RNA expression levels of VEGFα (ρ = 0.337), and the VEGFα expression levels were significantly correlated with TGFβ and TNFα., Conclusions: The AM hyperplasia was influenced by aging and could be a result of inflammation and fibrosis through cytokine secretion from the inflammatory cells and fibroblasts in the AM.- Published
- 2021
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49. Mucosa-associated lymphoid tissue lymphoma of the bladder: Case report.
- Author
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Yamamoto A, Nishikawa R, Hosoda R, Omura H, Tanaka T, and Muraoka K
- Abstract
We report the cases of patient with primary mucosa-associated lymphoid tissue (MALT) lymphoma of the bladder who successfully treated with local radiation therapy after transurethral resection of the bladder tumor. She maintains good activity of daily living without relapse. We recommend this strategy for localized MALT lymphoma of the bladder, because it provide better quality of life for elderly patients., Competing Interests: None declared., (© 2021 The Authors.)
- Published
- 2021
- Full Text
- View/download PDF
50. Different Antioxidative and Antiapoptotic Effects of Piceatannol and Resveratrol.
- Author
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Hosoda R, Hamada H, Uesugi D, Iwahara N, Nojima I, Horio Y, and Kuno A
- Subjects
- Cell Differentiation drug effects, Cell Line, Heme Oxygenase-1 metabolism, Humans, Mitochondria drug effects, Mitochondria metabolism, Myoblasts drug effects, Myoblasts metabolism, Oxidative Stress drug effects, Reactive Oxygen Species metabolism, Sirtuin 1 metabolism, Up-Regulation drug effects, Antioxidants pharmacology, Apoptosis drug effects, Resveratrol pharmacology, Stilbenes pharmacology
- Abstract
Resveratrol affords protection against reactive oxygen species (ROS)-related diseases via activation of SIRT1, an NAD
+ -dependent deacetylase. However, the low bioavailability of resveratrol limits its therapeutic applications. Since piceatannol is a hydroxyl analog of resveratrol with higher bioavailability, it could be an alternative to resveratrol. In this study, we compared the cytotoxicity, antioxidative activity, and mechanisms of cytoprotection of piceatannol with those of resveratrol. In C2C12 cells incubated with piceatannol, electrospray ionization mass spectrometry analysis showed that piceatannol was present in the intracellular fraction. A high concentration (50 μM) of piceatannol, but not resveratrol, induced mitochondrial depolarization and apoptosis. However, piceatannol at 10 μM inhibited the increase in mitochondrial ROS level induced by antimycin A, and this ROS reduction was greater than that by resveratrol. The reduction in hydrogen peroxide-induced ROS by piceatannol was also greater than that by resveratrol or vitamin C. Piceatannol reduced antimycin A-induced apoptosis more than did resveratrol. SIRT1 knockdown abolished the antiapoptotic activity of resveratrol, whereas it blocked only half of the antiapoptotic activity of piceatannol. Piceatannol, but not resveratrol, induced heme oxygenase-1 (HO1) expression, which was blocked by knockdown of the transcription factor NRF2, but not by SIRT1 knockdown. HO1 knockdown partially blocked the reduction of ROS by piceatannol. Furthermore, the antiapoptotic action of piceatannol was abolished by HO1 knockdown. Our results suggest that the therapeutic dose of piceatannol protects cells against mitochondrial ROS more than does resveratrol via SIRT1- and NRF2/HO1-dependent mechanisms. The activation of NRF2/HO1 could be an advantage of piceatannol compared with resveratrol for cytoprotection. SIGNIFICANCE STATEMENT: This study showed that piceatannol and resveratrol were different in cytotoxicity, oxidant-scavenging activities, and mechanisms of cytoprotection. Protection by piceatannol against apoptosis induced by reactive oxygen species was superior to that by resveratrol. In addition to the sirtuin 1-dependent pathway, piceatannol exerted nuclear factor erythroid 2-related factor 2/heme oxygenase-1-mediated antioxidative and antiapoptotic effects, which could be an advantage of piceatannol compared with resveratrol., Competing Interests: All authors have no conflicts of interest to declare., (Copyright © 2021 by The American Society for Pharmacology and Experimental Therapeutics.)- Published
- 2021
- Full Text
- View/download PDF
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