Search

The bovine leukemia virus (BLV) is an important infectious agent transmitted from cattle to humans. It is considered one of the oncogenic viruses in breast cancer, so an accurate detection of this virus is important. The study aimed to design a specific and sensitive method based on TaqMan® real-time polymerase chain reaction (RT-PCR) for BLV detection. Probes and primers were designed using bioinformatics software for a 108 pairs region of the BLV tax gene. Criteria employed for determining analytical sensitivity were prepared using in-vitro RNA transcriptions. The National Center for Biotechnology Information (NCBI), basic local alignment search tool (BLAST) databases various viral panels and genomic samples from healthy individuals (Qom Province, Iran in 2023) were used to verify analytical specificity and clinical specificity, respectively. This method can measure a minimum of 10 copies of DNA and RNA mL-1. Moreover, the assay is linear in the range of 100 - 109 copies mL-1. By testing negative specimens, the method specificity was 100%. The reproducibility results of the reaction were examined at the intra- and inter-assay comparison. In fact, 10 technical replicates of each concentration of the control sample were analyzed in each working reaction. Due to the locally made kit, exact sensitivity and specificity, rapid analysis, and relatively low cost, as compared to commercial kits of other countries, the method introduced in the present study could be suitable for accurate detection of the BLV. Also, the TaqMan® real-time PCR method could be detected in cattle and human and before malignant changes of breast cancer which could reduce infection and breast cancer. [ABSTRACT FROM AUTHOR]

Polyomaviruses are a group of small, double-stranded DNA viruses that are known to be associated with the development of certain human diseases, but there is evidence that these viruses might be associated with gastrointestinal (GI) cancers. Several polyomaviruses have been identified, such as JC polyomavirus (JCPyV), BK polyomavirus (BKPyV) and recently Merkel cell polyomavirus (MCPyV). Although the direct effects of polyomaviruses on transformation of human cells and cancer development are not clearly recognized, their association with certain human diseases including GI cancers has been proposed through several molecular and epidemiological studies. For example, JCPyV and BKPyV have been linked to colorectal cancer, as there is growing evidence of finding viral genomes in cancerous tissues. Nevertheless, the major role of JCPyV, BKPyV and MCPyV in colorectal cancer progression is still under extensive investigation, and further surveys is required to establish a conclusive cause-and-effect relationship. Understanding the role of these viruses in cancer development has significant implications for diagnosis, treatment, and prevention strategies. It seems that proving a causal link between polyomaviruses and GI cancers might provide a novel path for targeted therapies or design and development of specific therapeutic vaccines. In addition, performing research on the possible link can provide insights into the underlying molecular mechanisms of carcinogenesis, potentially leading to the identification of novel biomarkers. This review focuses on polyomaviruses, in particular a recently discovered polyomavirus, MCPyV, and their possible link with human gastrointestinal disorders. [ABSTRACT FROM AUTHOR]

Background and Objectives: Viral clearance studies are an essential part of a manufacturer's plan to ensure the safety of an injectable biologic product. In this way, viral safety is a critical quality attribute for biologics such as monoclonal antibodies (Mabs). Evaluation of virus purification by downstream processes is a key component of risk mitigation. In this study, the capability of continuous monoclonal antibody purification steps was evaluated in the process of instant monoclonal antibody purification in different stages of purification, and the amount of reduction or inactivation of each step was determined. Materials and Methods: Four enveloped and non-enveloped viral models VSV, Reovirus, EMCV, and HSV1 were used for spiking in selected samples in the designated tests, to have a comprehensive examination of the ability to clear the virus such as the type of genetic material, chemical resistance, and particle size. A TCID50 and qPCR methods were used to measure viral reduction. Two cell lines, Vero (African green monkey kidney) and L929 (Mouse fibroblast) were used for 4 model viruses propagation. The steps that were evaluated included 4 steps monoclonal antibody purification; cation exchange chromatography, acidic pH treatment, affinity chromatography, and nanofiltration. Results: The nano-filter stage showed the highest viral reduction and cation exchange chromatography showed the lowest reduction. The cumulative decrease using TCID50 is equal to 19.27 [log10] for all steps and for the qPCR method is equal to 12.47 [log10] in three steps of nano-filter, affinity chromatography, and ion exchange chromatography. Conclusion: The overall average reduction coefficient for all four model viruses is significantly high, which indicates the high capacity of the monoclonal antibody production process in inactivating and removing viruses leads to reducing the load of all four model viruses. [ABSTRACT FROM AUTHOR]

This paper scrutinizes the dialogical character of knowledge from the perspectives of Hegel's and Davidson's philosophies. First, it outlines their analogous trains of thought, particularly their "anti-representational" and "intersubjective" accounts of knowledge. Second, it draws a parallel between the two by discussing their contrasting views of the structure and goal of knowledge, showing that while Davidson advocates an open-ended, scheme-less empirical knowledge, Hegel maintains the notion of a (universal-rational) scheme and a goal-oriented dialectical process in which "the true is the whole." This section then critically traces their underlying disagreement to their divergent views on the nature of meaning, language, and thought. Finally, it argues that their views can be seen as complementary to two versions of dialectic, showing that while Hegel's approach, akin to Platonic dialectic, focuses on the self-contained and "Truth"-oriented "negotiational" movement of ideas, Davidson's, reminiscent of Socratic elenchus, emphasizes the truth-oriented 'conversational' interaction of subjects exchanging concepts. [ABSTRACT FROM AUTHOR]

In aesthetics/philosophy of art, Fichte did not produce works as great as Kant's Critique of the Power of Judgment or Hegel's Lectures on Aesthetics. As a result, it was long believed that he had no role to play in the aesthetics of German idealism. Nevertheless, there are a few works in which we can identify the materials for developing an innovative philosophy of art. In this article, it is argued that Fichte takes two fundamental steps in aesthetics: 1) by transferring the weight of the discussion of aesthetics to the philosophy of artistic creation, he makes, as it were, a Copernican revolution in aesthetics and thus transforms the aesthetics of taste into a philosophy of art based on the creative spirit; 2) he raises the status of aesthetics (in fact, the aesthetic sense) to the level of "a (or the) condition for the possibility of philosophy." [ABSTRACT FROM AUTHOR]

Aim: We investigated mutations in the BCP/PC regions and liver stiffness in three generations of hepatitis B (HBV) patients. Methods: In this study, viral load, liver function tests and liver stiffness (LSM) were examined in 90 chronic HBV patients, and BCP/PC regions were amplified by PCR. Results: There was no statistically significant difference between G1896 mutation and DNA level in any of the groups. The results indicated that G1896A and G1764A were the most frequent mutations in three generations (mothers; 33.4%), and in children (two generations; 45%). Conclusion: The three- (four cases) and two-generation (six patients) groups had similar mutations. Together with LSM outcomes, this may play a crucial part in the natural course of HBV infection. [ABSTRACT FROM AUTHOR]

Introduction: Hypoxia context is highly specific for tumors and represents a unique niche which is not found elsewhere in the body. Clostridium novyi is an obligate anaerobic bacterium. It has a potential to treat tumors. The aim of this study was to produce the C. novyi nontoxic spores and to investigate its oncolytic effect on breast cancer in mice model. Methods: Primarily, the lethal toxin gene in C. novy i type B was removed. Colonies were isolated using PCR testing. To assure the removal of alpha-toxin, plasmid extraction and in vivo assay were conducted. Next, to treat breast cancer model in different sizes of tumors, a single dose of spores of C. novyi nontoxic was tested. Results: The results denoted that C. novyi nontoxic lost lethal toxin and a--ppeared to be safe. For smaller than 1000 mm3 tumors, a single dose of C. novyi nontoxic was able to cure 100% of mice bearing breast tumors. Hence the mice remained free of tumor relapse. Tumors larger than 1000 mm3 were not cured by a single dose- of C. novyi nontoxic treatment. Conclusion: The experiment concluded that the C. novyi nontoxic might be a suitable and safe candidate, a novel therapeutic approach to encounter such hypoxic regions in the center of tumors. Research also showed that bacteriolytic therapy by C. novyi nontoxic could lead to regression in small tumor. [ABSTRACT FROM AUTHOR]