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Additional file 1:. Fig.S1 Tau oligomer preparation and control dot-blots of brain lysates without primary antibody OC. a Tau oligomer preparation by size exclusion chromatography. b Oligomer native gel electrophoresis visualized by coomassie stain, followed by western blot using HT-7 antibody. c Control dot-blots of brain lysates were probed without primary antibody OC, or using β-actin as a control. The blots were stained for total protein with Ponceau S simultaneously. Fig.S2. The motor performance of mice in behavioral test. a The travelled distance in probe trial of MWM. b The swim speed in MWM. c The travelled distance in Y-maze. d The velocity of mice in Y-maze. e The travelled distance in NOR. f The total exploration time in NOR. Fig.S3 Rutin rescues synapse loss in Tau-P301S mice. PSD95 immunostaining and synaptophysin immunostaining in the brains of Tau-P301S mice and their WT littermates treated with rutin or vehicle. (Scale bar: 10 μm). Fig.S4 Rutin prevents microglial synapse engulfment in Tau-P301S mice. Representative images show the engulfed PSD95 (red) puncta within Iba-1+ (green) microglial cells in the brains of Tau-P301S mice and their WT littermates treated with rutin or vehicle. (Scale bar: cyan, 25 μm; white, 20 μm).