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8 results on '"Hou, Wei-Na"'

4. Satellite RNA reduces expression of the 2b suppressor protein resulting in the attenuation of symptoms caused by Cucumber mosaic virus infection

Author

HOU, WEI‐NA, DUAN, CHENG‐GUO, FANG, RONG‐XIANG, ZHOU, XIAO‐YANG, and GUO, HUI‐SHAN

Subjects
Viral Proteins, DNA, Complementary, Tobacco, Arabidopsis, Agrobacterium, RNA, Satellite, Original Articles, Genome, Viral, RNA, Small Interfering, Genes, Suppressor, Cucumovirus, Plant Diseases
Abstract

Satellite RNAs (satRNAs) depend on cognate helper viruses for replication, encapsidation, movement and transmission. Many satRNAs with different symptom modulation effects have been reported. The pathogenicity of satRNAs is thought to be the result of a direct interaction among the satRNA, helper viruses and host factors by unknown mechanisms. To understand the effect of satRNA of Cucumber mosaic virus (a severe field ShanDong strain, SD-CMV) on pathogenicity, and the possible involvement of host RNA silencing pathways in pathogenicity, we constructed biologically active CMV cDNA clones and a CMV-Δ2b mutant lacking the open reading frame of 2b, a silencing suppressor protein, in order to infect Nicotiana benthamiana and Arabidopsis with or without SD-satRNA. We found that SD-satRNA reduced the accumulation of the 2b protein and its coding RNA4A and attenuated the yellowing caused by SD-CMV infection. Small RNA analysis indicated that the 2b protein interfered with RNA silencing, specifically in the synthesis of CMV RNA3-derived small interfering RNAs (R3-siRNAs). The accumulation of R3-siRNAs in CMV-Δ2b infection was reduced in the presence of satRNA, for which greater accumulation of satRNA-derived siRNAs (satsiRNAs) was detected. Our results suggest that abundant SD-satRNA serving as target for RNA silencing may play a role in protecting helper CMV RNA, especially, subgenomic RNA4, from being targeted by RNA silencing. This compensates for the increase in RNA silencing resulting from the reduction in expression of the 2b suppressor in the presence of satRNA. Our data provide evidence that a plant silencing mechanism is involved in the pathogenicity of satRNA.

Published
2011

8. [Prognostic significance of coagulation disorders in children with hemophagocytic syndrome].

Author

Guo J, Wang L, Hou WN, Bai ST, Sun SK, and Sheng GY

Subjects
Adolescent, Child, Child, Preschool, Etoposide therapeutic use, Female, Humans, Infant, Lymphohistiocytosis, Hemophagocytic blood, Lymphohistiocytosis, Hemophagocytic complications, Lymphohistiocytosis, Hemophagocytic drug therapy, Male, Prognosis, Retrospective Studies, Disseminated Intravascular Coagulation mortality, Lymphohistiocytosis, Hemophagocytic mortality
Abstract

Objective: To study the prognostic significance of coagulation disorders in children with hemophagocytic syndrome (HPS)., Methods: Thirty-five children with HPS were retrospectively studied to analyze the etiology, clinical characteristics, laboratory results and treatment outcomes., Results: After treatment, 27 of the 35 HPS patients survived, and the other 8 cases died. All cases were treated according to the HLH-2004 protocol, but etoposide (VP-16) was not used in 10 of them. The response rate in patients who received VP-16 (22/25, 88%) was significantly higher than that in those not receiving VP-16 (5/10, 50%) (P<0.05). Compared with the survival group, the dead group had significantly lower platelet count, fibrinogen level, and VP-16 utilization rate (P<0.05) but significantly longer activated partial thromboplastin time and prothrombin time (P<0.05)., Conclusions: Coagulation function can be used as an indicator of disease outcome. It is essential for improving the clinical outcome of HPS to monitor the coagulation function during treatment, detect and correct abnormalities in time, and provide treatment strictly according to the HLH-2004 protocol.

Published
2014

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