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10 results on '"Hou, Xun-Ya"'

2. A 36-year-old Chinese man with high fever, abdominal pain, watery diarrhea, and myalgia

Author

Ross, Allen G., Hou, Xun-ya, Chen, Si-xi, McManus, Donald P., and Li, Yuesheng

Subjects
Abdominal pain -- Case studies, Fever -- Case studies, Hyperthermia -- Case studies, Diarrhea -- Case studies, Myalgia -- Case studies, Schistosomiasis -- Diagnosis, Schistosomiasis -- Care and treatment, Schistosomiasis -- Case studies, Health, Health care industry
Published
2010

4. Primary Establishment of LAMP Method for Cyprinid Herpesvirus 2 Detection

Author

Shan Zhen Peng, Ze Xiao Yang, Yin Wang, Kai Yu Wang, Hou Xun Ya, Xue Ping Yao, Bo Wang, Gui Li Li, and Ran Yang Ren

Subjects
biology, Chemistry, DNA polymerase, Loop-mediated isothermal amplification, General Medicine, biology.organism_classification, Molecular biology, Aeromonas hydrophila, chemistry.chemical_compound, GenBank, Agarose gel electrophoresis, biology.protein, Overlap extension polymerase chain reaction, DNA, Aeromonas veronii
Abstract

A new loop-mediated isothermal amplification (LAMP) method for the detection of Cyprinid herpesvirus 2 (CyHV-2) was developed. Following the synthesis in vitro of a 413 bp DNA fragment of CyHV2 DNA-dependent DNA polymerase gene by overlap extension PCR using 11 overlapping oligo primers designed according to the gensome sequences information of CyHV2 published in GenBank, a set of 6 LAMP primers were designed based on the synthesized DNA fragment, and the LAMP assay was primarily established after preparation of target gene fragments, optimization of reaction conditions, products identification, sensitivity and specificity tests. Results showed the developed LAMP assay was a rapid, specific and sensitive method for CyHV2 detection, with a ladder-like pattern of amplification bands from about 177 bp incubation at 64°C for 30 min by using agarose gel electrophoresis. The detection limit could reach about 15 copies of cloned viral genomic fragments, and no cross reaction with Aeromonas veronii, Aeromonas hydrophila, Pseudomonas fluorescens and Streptococcus common pathogens isolated from fish detection by this approach.

Published
2014

5. A randomized, double-blind, placebo-controlled trial of safety and efficacy of combined praziquantel and artemether treatment for acute schistosomiasis japonica in China

Author

Hou, Xun-Ya, McManus, Donald P, Gray, Darren, Balen, Julie, Luo, Xin-Song, He, Yongkang, Ellis, Magda, Williams, Gail, Li, Yue-Sheng, Hou, Xun-Ya, McManus, Donald P, Gray, Darren, Balen, Julie, Luo, Xin-Song, He, Yongkang, Ellis, Magda, Williams, Gail, and Li, Yue-Sheng

Abstract

Objective: To evaluate the safety and efficacy of combining artemether (AM) and praziquantel (PZQ) in different regimens for treating acute schistosomiasis japonica. Methods: We undertook a randomized, double-blind, placebo-controlled trial within four specialized schistosomiasis hospitals in the Dongting Lake region, Hunan province, China, between May 2003 and December 2005. Study participants were randomized into one of four treatment regimes: group A received 60 mg/kg PZQ + 6 mg/kg AM; group B received 60 mg/kg PZQ + AM placebo; group C received 120 mg/kg PZQ + 6 mg/kg AM; and group D received 120 mg/kg PZQ + AM placebo. All participants were followed up over a 45-day period. The primary endpoint of the trial was human infection status (determined by positive stool examination). Secondary endpoints involved clinical observations and blood biochemistry, including monitoring haemoglobin and alanine aminotransferase levels over time. Findings: Treatment efficacies of the four different treatment regimens were 98.0%, 96.4%, 97.7% and 95.7% for group A, B, C, and D respectively (P > 0.05). The group B had a greater treatment efficacy (96.4%) than the group D (95.7%) (P > 0.05). Group A treatment was better for clearance of fever (P < 0.05) and resulted in a shorter hospitalization time (P < 0.05). Conclusion: This is the first report of a randomized, double-blind, placebo-controlled trial for evaluating combined chemotherapy with AM and two different dosages (60 mg/kg and 120 mg/kg) of PZQ in the treatment of acute schistosomiasis japonica in China. The combination of AM and PZQ chemotherapy did not improve treatment efficacy compared with PZQ alone. PZQ given as a dosage of 60 mg/kg (1 day, 3 x 20 mg/kg doses at 4-5 hour intervals) may be as effective as a dosage of 120 mg/kg (6 days, 20 mg/kg for each day split into 3 doses at 4-5 hour intervals).

Published
2008

8. [Role of socio-economy and management in sustainable transmission control of schistosomiasis in Taoyuan County, Hunan Province, China].

Author

Luo ZH, Li SM, Zhou XN, Yi P, Ren GH, Franziska B, Zhao ZY, Hou XY, Ren MY, Li YY, Dong RL, Zeng JY, and She SP

Subjects
Adolescent, Animals, Cattle, Child, China, Endemic Diseases prevention & control, Health Knowledge, Attitudes, Practice, Humans, Rural Population statistics & numerical data, Schistosomiasis epidemiology, Communicable Disease Control methods, Economic Development statistics & numerical data, Public Health Administration statistics & numerical data, Schistosomiasis prevention & control, Schistosomiasis transmission
Abstract

Objective: To clarify the role of socio-economy and management in the sustainable schistosomiasis-control in Taoyuan County, an endemic area in hilly region, Hunan Province, China., Methods: From 1996 to 2011, the data of socio-economy, the management of schistosomiasis control organizations, environment, and the changes in schistosomiasis prevalence were collected in Taoyuan County where schistosomiasis transmission had been controlled since 2008. A sampling survey of schistosomiasis prevalence of human and bovine was performed in 2011 to verify the current status of schistosomiasis transmission. All the data were analyzed statistically to evaluate the role of socio-economy and management in the sustainable schistosomiasis control., Results: During the period from 1998 to 2012, the socio-economy including the residents' productive mode and daily life in Taoyuan County improved dramatically, but the recurrence risk of schistosomiasis endemic still existed due to the retuning of out-going workers and the migrating population. Moreover, the introduction of exotic species of plants and animals may increase the risk. The low running cost of schistosomiasis control organization as well as the efficient and adequate resource allocation in the county was in line with the national requirement to strengthen the rural grass-roots public health system., Conclusion: The harmonious development of socio-economy and the scientific and efficient health system in Taoyuan County are the key factors for the sustainable transmission control of schistosomiasis.

Published
2013

9. [Study on the relationship between the degree of periportal fibrosis, hepatic parenchymatous fibrosis and diameter of portal vein in Schistosoma japonicum infection].

Author

Fu X, Luo XS, Hou XY, He HB, Zhou J, Wang YY, He YK, Alain D, and Li YS

Subjects
Adult, China epidemiology, Female, Humans, Male, Schistosomiasis epidemiology, Ultrasonography, Liver Cirrhosis diagnostic imaging, Liver Cirrhosis pathology, Portal Vein diagnostic imaging, Schistosomiasis diagnostic imaging
Abstract

The degree of periportal fibrosis, hepatic parenchymatous fibrosis and the diameter of portal vein in fishermen from highly endemic area of schistosomiasis japonica in Dongting Lake region were measured. The results showed a significant correlation between the degree of periportal fibrosis and parenchymatous fibrosis and the portal venous diameter with a correlation coefficient of 0.375 and 0.332 respectively. The authors consider that the diameter of the portal vein can be used to assess the hepatic morbidity of patients.

Published
2006

10. [Preliminary observation on specific antibody level and reduction of ovulation induced by recombinant Schistosoma japonicum 26 kDa GST antigen in water buffaloes].

Author

He YK, Liu SX, Yu XL, Song GC, Xu YX, Cao JP, Yang RQ, Hou XY, and Zhang XY

Subjects
Animals, Buffaloes, Feces parasitology, Fertility, Parasite Egg Count veterinary, Random Allocation, Recombinant Proteins immunology, Schistosomiasis japonica immunology, Schistosomiasis japonica parasitology, Vaccination veterinary, Vaccines, Synthetic immunology, Antibodies, Helminth blood, Antigens, Helminth immunology, Glutathione Transferase immunology, Schistosoma japonicum immunology, Schistosomiasis japonica veterinary
Abstract

Objective: To observe the specific antibody level and reduction of egg-laying induced by a recombinant Schistosoma japonicum 26 kDa GST antigen (rSjc26 GST) in water buffaloes., Methods: 20 water buffaloes were randomly divided into two groups, the vaccination group and control group, with 10 buffaloes each. The subjects in vaccination group were immunized with rSjc26 GST antigen while the control received adjuvant only. After challenged with S. japonicum cercariae, the anti-rSjc26 GST antibody level and the numbers of eggs and miracidia in stool were detected., Results: The anti-rSjc26 GST antibody appeared 1 month after immunization with rSjc26 GST antigen and maintained a high level for 12 months. Numbers of eggs (EPG) and miracidia (MPG) in vaccination group were significantly lower than those in control group during the period of day 50 to day 90 post-challenge. However, EPG and MPG tended to decrease starting from day 100 post challenge in both groups. The difference of EPG and MPG between the two groups diminished progressively, and both groups showed zero egg count from day 330 on post challenge., Conclusion: The specific anti-rSjc26 GST antibody was detected in vaccinated water buffaloes and maintained a high level for 12 months. The vaccination showed a significant effect to the reduction of ovulation in the first three months after S. japonicum cercariae challenge.

Published
2002

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