21 results on '"HouJun Jia"'
Search Results
2. Preoperative inflammation and nutrition-based comprehensive biomarker for predicting prognosis in resectable colorectal cancer
- Author
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Hao Cai, Jiancheng Li, Yu Chen, Qiao Zhang, Yang Liu, and Houjun Jia
- Subjects
colorectal cancer ,Onodera’ s prognostic nutritional index ,prognosis ,systemic inflammation response index ,hematological markers ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundColorectal cancer (CRC) remains a major global health concern, with significant morbidity and mortality rates. In this study, we aimed to develop a comprehensive blood indicator based on systemic inflammation and nutritional condition to predict the prognosis of resectable CRC patients.MethodsA retrospective cohort of 210 CRC patients who underwent radical resection at the First Affiliated Hospital of Chongqing Medical University, China, between January 2015 and December 2017, was included in the analysis. Baseline characteristics, preoperative blood markers, including neutrophil count, monocyte count, lymphocyte count, platelets, albumin, and CEA were retrospectively reviewed. Various blood indicators, such as NLR, PLR, MLR, SIRI and OPNI were calculated. The least absolute shrinkage and selection operator (LASSO) method was employed to select indicators to establish a novel comprehensive biomarker (named PSI). Kaplan-Meier survival curves and log-rank tests were used to evaluate the prognostic impact of preoperative OPNI, SIRI, and PSI. Univariate and multivariate Cox regression model were conducted to identify independent prognostic factors for CRC. The receiver operating characteristic (ROC) method assessed the predictive ability of PSI, stage, OPNI, and SIRI.ResultsPatients with higher preoperative OPNI and lower SIRI values had significantly better overall survival (OS). PSI was identified as an independent prognostic factor for OS in both univariate and multivariate analysis. Patients with medium (28.3-43.4) and high (>43.4) PSI scores exhibited superior OS compared to those with low (≤ 28.3) PSI scores. PSI showed higher predictive ability (AUC: 0.734) than individual indicators alone (OPNI: 0.721, SIRI: 0.645, stage: 0.635).ConclusionThe novel indicator, PSI, based on preoperative SIRI and OPNI, demonstrated significant prognostic value for resectable CRC patients. PSI outperformed individual indicators and could serve as a reliable tool for prognostic evaluation in CRC patients.
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- 2023
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- View/download PDF
3. Telomere Length as a Prognostic Factor for Overall Survival in Colorectal Cancer Patients
- Author
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Houjun Jia and Ziwei Wang
- Subjects
Colorectal cancer ,Telomere length ,Prognosis ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: The stabilization of telomere length has important roles in the carcinogenesis of colorectal cancer. A systemic review and meta-analysis of published studies was performed to assess the prognostic role of telomere length in colorectal cancer. Methods: Pubmed and Embase were searched for eligible studies on the association between telomere length and overall survival in colorectal cancer patients. The pooled hazard ratio (HR) and corresponding 95% confidence intervals (95%CI) was calculated using fixed-effects or random-effects model according to the magnitude of between-study heterogeneity. Results: Seven individual studies with a total of 956 colorectal cancer patients were included. Long telomere length in cancer tissues was marginally associated with poorer overall survival (Random-effects HR = 1.85, 95% 0.90 to 3.83, P = 0.09). When using studies with adjusted estimates, long telomere length in cancer tissues was independently and significantly associated with poorer overall survival (Fixed-effects HR = 2.70, 95% 1.51 to 4.84, P = 0.001). However, short telomere length in peripheral blood leukocytes was independently and significantly associated with poorer overall survival (Fixed-effects HR = 2.01, 95% 1.46 to 2.77, P Conclusions: There is some evidence for telomere length as a prognostic factor for overall survival in colorectal cancer patients. More studies with large number of participants are needed to further assess the prognostic significance of telomere length in colorectal cancer patients.
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- 2016
- Full Text
- View/download PDF
4. Selection of Therapeutic Strategies after Preoperative Neoadjuvant Chemoradiotherapy for Rectal Cancer
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Youzhong Ke, Yuan Liu, Hao Zhang, and Houjun Jia
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Automotive Engineering - Abstract
Rectal cancer is one of the most common malignant tumors in China, which is mainly middle and low rectal cancer. Due to the particularity of the physiological and anatomical location of the rectum and the neglect of the relevant clinical symptoms, patients with rectal cancer in real life often have the local progression stage. A large number of studies have shown that neoadjuvant chemoradiotherapy should be performed in such patients, to achieve tumor downstaging before rectal cancer surgery. In this study, different treatment measures for rectal cancer patients after neoadjuvant chemoradiotherapy are presented.
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- 2023
5. The lncRNA BCYRN1 Functions as an Oncogene in Human Glioma by Downregulating miR-125a-5p in vitro
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Qian Liu, Shujuan Zhu, Jie Sheng, Dulei Xiang, Yuxiang Long, Wei Yu, Xin He, Houjun Jia, and Kejian Wang
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0301 basic medicine ,Gene knockdown ,Oncogene ,Competing endogenous RNA ,Endogeny ,Biology ,medicine.disease ,In vitro ,law.invention ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,Downregulation and upregulation ,law ,030220 oncology & carcinogenesis ,Glioma ,medicine ,Cancer research ,Suppressor - Abstract
Introduction Numerous studies have demonstrated that long noncoding RNAs (lncRNAs) are deregulated in many cancers and exert their functions through multiple cancer-related biological processes. Glioma is the most common primary malignant central nervous system tumor and has a high fatality rate in adults. In current study, we aimed to determine the role and functional mechanism of the lncRNA BCYRN1 in glioma. Methods Gain-of-function and loss-of function approaches were used to investigate the function of BCYRN1. The effects of BCYRN1 on glioma cell proliferation, migration and invasion were evaluated using MTS, Transwell and wound-healing assays. The correlation between the expression of BCYRN1 and miR-125a-5p was verified by quantitative real-time PCR. Results The upregulation of BCYRN1 promoted the proliferation, migration and invasion of glioma cells. Meanwhile, the knockdown of BCYRN1 had the opposite effects. BCYRN1 was negatively correlated with miR-125a-5p. Additionally, TAZ, the endogenous target of miR-125a-5p, could be regulated by BCYRN1 in RNA and protein levels. A miR-125a-5p inhibitor restored BCYRN1 siRNA function in glioma. Conclusion The present study indicates that BCYRN1 promotes glioma cell proliferation, invasion and migration in vitro. Mechanistically, upregulated expression of BCYRN1 in glioma acts as a sponge to sequester the endogenous tumor suppressor miR-125a-5p and to further increase the expression TAZ. Our findings suggest that BCYRN1 is a novel oncogene and a new therapeutic target for glioma.
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- 2020
6. Wang's Forceps-Assisted Percutaneous Insertion and Fixation of Peritoneal Dialysis Catheter
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Xinghua Chen, Cuizhi Xiang, Jili Zhu, Houjun Jia, Guohua Ding, Yujuan Wang, Huiming Wang, and Cheng Chen
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medicine.medical_specialty ,Percutaneous ,Catheter insertion ,business.industry ,medicine.medical_treatment ,Forceps ,030232 urology & nephrology ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,General Medicine ,030204 cardiovascular system & hematology ,Peritoneal dialysis ,Surgery ,End stage renal disease ,Biomaterials ,03 medical and health sciences ,Fixation (surgical) ,Catheter ,0302 clinical medicine ,medicine ,Peritoneal dialysis catheter ,business - Abstract
Percutaneous insertion of peritoneal dialysis catheters is theoretically most preferred by nephrologists because of the advantages of bedside performing, surgery independence, and minimal injury over other procedures of catheter placement such as open surgical dissection or laparoscopic operation. However, blindly placing catheters in the percutaneous procedure brings the risk of catheter malposition or bowel perforation; this largely retarded it's implementation. We had previously developed a novel technique termed "Wang's forceps-assisted catheter insertion and fixation," which had been successfully applied in the open surgical catheter insertion and displaced catheter reposition in our center. In this study, we further explored the possibility of applying the Wang's forceps in the procedure of percutaneous catheter insertion both in porcine model and patients with end stage renal disease (ESRD). A total of three miniature pigs successfully received percutaneous catheter insertion using Seldinger's technique with Wang's forceps assistance. The catheters were all placed in the right position and functioning well in dialysate drainage. This novel method of percutaneous catheter insertion was then performed on 20 ESRD patients. The procedure showed effective time-saving with the average operating time of 29.2 ± 3.53 min and was well tolerated by patients with minimal pain and injury. During a follow-up time of 6 months, no complications of catheter displacement, leakage, or blockade occurred. Our preliminary observation demonstrates that utilization of Wang's forceps in a percutaneous procedure conferred benefits of accurately placing and fixing catheters while preserving the merits of minimal invasion and simple performance.
- Published
- 2018
7. Identification of the appropriate fixation site to avoid peritoneal catheter migration based on a mechanical analysis
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Cuizhi Xiang, Jili Zhu, Xinghua Chen, Houjun Jia, Yao Zou, Huiming Wang, Yujuan Wang, and Guohua Ding
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Models, Anatomic ,medicine.medical_specialty ,Peritoneal dialysis ,finite element method ,0206 medical engineering ,030232 urology & nephrology ,Prosthesis Retention ,02 engineering and technology ,Critical Care and Intensive Care Medicine ,Abdominal wall ,03 medical and health sciences ,Fixation (surgical) ,Catheters, Indwelling ,0302 clinical medicine ,Peritoneal Dialysis, Continuous Ambulatory ,Laboratory Study ,Materials Testing ,medicine ,Perpendicular ,Humans ,Computer Simulation ,Displacement (orthopedic surgery) ,business.industry ,General Medicine ,020601 biomedical engineering ,Finite element method ,abdominal wall fixation ,Prosthesis Failure ,Surgery ,Catheter ,medicine.anatomical_structure ,Nephrology ,Cuff ,Dynamic pressure ,business ,Biomedical engineering - Abstract
Aim: To conduct mechanical analysis on the relationship between abdominal wall fixation point and the displacement of catheter top, and establish the finite element model for the complex forces and conditions that the catheter wears in human abdominal cavity, in order to provide the scientific basis for optimizing the catheter position in abdominal wall fixation method. Methods: Using the PIPE59 finite elements to divide units, and taking the lower part of catheter, that is, below interior polyester cuff to simulate and compute the displacement formula. Results: The whole model includes a total of 1701 units. Periodic load was used to simulate the dynamic pressure that peritoneal dialysis catheter gets in abdominal cavity. The load direction was perpendicular to the catheter axis. We used pressure amplitude, duration and frequency as the boundary conditions, and adjusted the fixation point of the catheter lower part at the same time, thus calculating the extreme displacement value of the catheter top end with changing parameter conditions. We also did fitted regression on the results and obtained the displacement formula: y = 0.2 × 0.87x (y: the end displacement of peritoneal dialysis catheter, x: the distance between fixation point and the interior polyester cuff), R2: .982. Simulation the catheter maximal displacement on flat surface demonstrated that additional catheter fixation at the site of 9 cm or more below the internal cuff significantly restricted the catheter migration. Conclusions: The optimal position of fixation point in peritoneal dialysis is about 9 cm away from the interior polyester cuff.
- Published
- 2017
8. The lncRNA BCYRN1 Functions as an Oncogene in Human Glioma by Downregulating miR-125a-5p in vitro
- Author
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Wei, Yu, Dulei, Xiang, Houjun, Jia, Xin, He, Jie, Sheng, Yuxiang, Long, Shujuan, Zhu, Kejian, Wang, and Qian, Liu
- Subjects
TAZ ,glioma ,BCYRN1 ,miR-125a-5p ,ceRNA ,Original Research - Abstract
Introduction Numerous studies have demonstrated that long noncoding RNAs (lncRNAs) are deregulated in many cancers and exert their functions through multiple cancer-related biological processes. Glioma is the most common primary malignant central nervous system tumor and has a high fatality rate in adults. In current study, we aimed to determine the role and functional mechanism of the lncRNA BCYRN1 in glioma. Methods Gain-of-function and loss-of function approaches were used to investigate the function of BCYRN1. The effects of BCYRN1 on glioma cell proliferation, migration and invasion were evaluated using MTS, Transwell and wound-healing assays. The correlation between the expression of BCYRN1 and miR-125a-5p was verified by quantitative real-time PCR. Results The upregulation of BCYRN1 promoted the proliferation, migration and invasion of glioma cells. Meanwhile, the knockdown of BCYRN1 had the opposite effects. BCYRN1 was negatively correlated with miR-125a-5p. Additionally, TAZ, the endogenous target of miR-125a-5p, could be regulated by BCYRN1 in RNA and protein levels. A miR-125a-5p inhibitor restored BCYRN1 siRNA function in glioma. Conclusion The present study indicates that BCYRN1 promotes glioma cell proliferation, invasion and migration in vitro. Mechanistically, upregulated expression of BCYRN1 in glioma acts as a sponge to sequester the endogenous tumor suppressor miR-125a-5p and to further increase the expression TAZ. Our findings suggest that BCYRN1 is a novel oncogene and a new therapeutic target for glioma.
- Published
- 2019
9. Telomere Length as a Prognostic Factor for Overall Survival in Colorectal Cancer Patients
- Author
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Ziwei Wang and Houjun Jia
- Subjects
0301 basic medicine ,Oncology ,medicine.medical_specialty ,Databases, Factual ,Physiology ,Colorectal cancer ,medicine.disease_cause ,Bioinformatics ,lcsh:Physiology ,lcsh:Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Leukocytes ,medicine ,Humans ,lcsh:QD415-436 ,Telomere Shortening ,Survival analysis ,Telomere length ,lcsh:QP1-981 ,business.industry ,Hazard ratio ,Cancer ,Telomere ,Prognosis ,medicine.disease ,Survival Analysis ,Confidence interval ,030104 developmental biology ,030220 oncology & carcinogenesis ,Meta-analysis ,Colorectal Neoplasms ,Carcinogenesis ,business - Abstract
Background/Aims: The stabilization of telomere length has important roles in the carcinogenesis of colorectal cancer. A systemic review and meta-analysis of published studies was performed to assess the prognostic role of telomere length in colorectal cancer. Methods: Pubmed and Embase were searched for eligible studies on the association between telomere length and overall survival in colorectal cancer patients. The pooled hazard ratio (HR) and corresponding 95% confidence intervals (95%CI) was calculated using fixed-effects or random-effects model according to the magnitude of between-study heterogeneity. Results: Seven individual studies with a total of 956 colorectal cancer patients were included. Long telomere length in cancer tissues was marginally associated with poorer overall survival (Random-effects HR = 1.85, 95% 0.90 to 3.83, P = 0.09). When using studies with adjusted estimates, long telomere length in cancer tissues was independently and significantly associated with poorer overall survival (Fixed-effects HR = 2.70, 95% 1.51 to 4.84, P = 0.001). However, short telomere length in peripheral blood leukocytes was independently and significantly associated with poorer overall survival (Fixed-effects HR = 2.01, 95% 1.46 to 2.77, P < 0.001). Conclusions: There is some evidence for telomere length as a prognostic factor for overall survival in colorectal cancer patients. More studies with large number of participants are needed to further assess the prognostic significance of telomere length in colorectal cancer patients.
- Published
- 2016
10. Wang's Forceps-Assisted Percutaneous Insertion and Fixation of Peritoneal Dialysis Catheter
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Huiming, Wang, Yujuan, Wang, Jili, Zhu, Xinghua, Chen, Cheng, Chen, Cuizhi, Xiang, Houjun, Jia, and Guohua, Ding
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Adult ,Male ,Swine ,Equipment Design ,Middle Aged ,Surgical Instruments ,Catheterization ,Disease Models, Animal ,Catheters, Indwelling ,Animals ,Humans ,Kidney Failure, Chronic ,Female ,Peritoneal Dialysis ,Aged - Abstract
Percutaneous insertion of peritoneal dialysis catheters is theoretically most preferred by nephrologists because of the advantages of bedside performing, surgery independence, and minimal injury over other procedures of catheter placement such as open surgical dissection or laparoscopic operation. However, blindly placing catheters in the percutaneous procedure brings the risk of catheter malposition or bowel perforation; this largely retarded it's implementation. We had previously developed a novel technique termed "Wang's forceps-assisted catheter insertion and fixation," which had been successfully applied in the open surgical catheter insertion and displaced catheter reposition in our center. In this study, we further explored the possibility of applying the Wang's forceps in the procedure of percutaneous catheter insertion both in porcine model and patients with end stage renal disease (ESRD). A total of three miniature pigs successfully received percutaneous catheter insertion using Seldinger's technique with Wang's forceps assistance. The catheters were all placed in the right position and functioning well in dialysate drainage. This novel method of percutaneous catheter insertion was then performed on 20 ESRD patients. The procedure showed effective time-saving with the average operating time of 29.2 ± 3.53 min and was well tolerated by patients with minimal pain and injury. During a follow-up time of 6 months, no complications of catheter displacement, leakage, or blockade occurred. Our preliminary observation demonstrates that utilization of Wang's forceps in a percutaneous procedure conferred benefits of accurately placing and fixing catheters while preserving the merits of minimal invasion and simple performance.
- Published
- 2017
11. A Study on the Mechanism of Low-Expressed Cancer Stem Cell Marker Lgr5 in Inhibition of the Proliferation and Invasion of Colorectal Carcinoma
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Ziwei Wang, Qipeng Zhou, Lin Xiang, and Houjun Jia
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Male ,Biopsy ,Biophysics ,Biology ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Receptors, G-Protein-Coupled ,Cell Movement ,Cancer stem cell ,Cell Line, Tumor ,medicine ,Carcinoma ,Humans ,RNA, Messenger ,RNA, Small Interfering ,Aged ,Cell Proliferation ,medicine.diagnostic_test ,Cell growth ,LGR5 ,Colonoscopy ,Cell Biology ,General Medicine ,Transfection ,Middle Aged ,Cadherins ,medicine.disease ,Molecular biology ,Cell culture ,Lymphatic Metastasis ,Neoplastic Stem Cells ,Female ,RNA Interference ,Stem cell ,Colorectal Neoplasms - Abstract
The present study intends to explore the influence of Lgr5 as a marker of tumor stem cells after siRNA interference on the proliferation and invasion of colorectal carcinoma (CRC) and its mechanism. The tissue samples were taken for biopsy from 32 cases of patients and 32 cases of normal subjects by colonoscopy. Real-time quantitative PCR was used to detect the differential expression of Lgr5. After siRNA interference of Lgr5 in CRC cell line CT-26 cells, RT-PCR method was used to detect the mRNA expression level of Lgr5 after interference of CT-26 cells. CCK8 method was used to observe the influence of Lgr5 interference on the proliferation, colony formation, and invasion of CT-26 cells. RT-PCR and Western blot were used to detect the E-cadherin mRNA and protein levels in CT-26 cells. Lgr5 expression level in CRC tissue was significantly higher than that in the corresponding para-carcinoma tissue and the control group, and the differences were statistically significant (P
- Published
- 2015
12. Inferring decelerated land subsidence and groundwater storage dynamics in Tianjin–Langfang using Sentinel-1 InSAR
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Xuguo Shi, Tongtong Zhu, Wei Tang, Mi Jiang, Houjun Jiang, Chen Yang, Wei Zhan, Zutao Ming, and Shaocheng Zhang
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north china plain ,aquifer parameter ,south-to-north water transfer project ,groundwater storage ,subsidence ,Mathematical geography. Cartography ,GA1-1776 - Abstract
To meet the growing demand for socioeconomic development, a large amount of groundwater is extracted from confined aquifers worldwide. The North China Plain has experienced considerable groundwater depletion and subsidence during the past six decades. In this study, we use Sentinel-1A/B SAR images from 2015 to 2020 to map the ground subsidence of the Tianjin–Langfang area. Three subsiding zones centered at Guangyang, Wuqing–Bazhou, and Jinghai are identified with maximum subsidence rates of 98.1, 121.8, and 104.7 mm/yr. Seasonal and long-term signals are separated from time series subsidence and hydraulic measurements using continuous wavelet transform to retrieve aquifer parameters. The long-term subsidence, which fits well with an exponential decaying model, remarkably slows down in our study area. The elastic skeletal storage coefficients range between 0.52×10−3 and 9.66×10−3. We then retrieve the spatial–temporal variations of total groundwater storage, recoverable groundwater storage, and irreversible groundwater storage. Groundwater storage depletion rates are apparently reducing, which benefits from the operation of the South-to-North Water Transfer Project and local groundwater management practices.
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- 2022
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13. Abstract 3453: PAK6 localizes to centrosome and modulates centrosome homeostasis
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Jianning Wei, Vijaya Iragavarapu, John Mallow, Houjun Jia, Michael D. Perttunen, Michael L. Lu, and Danda Chapagai
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Genome instability ,Cancer Research ,Oncology ,Centrosome ,Microtubule ,Cancer cell ,LNCaP ,Biology ,Aster (cell biology) ,Cytokinesis ,Cell biology ,Centrosome localization - Abstract
Centrosome abnormalities are linked to genomic instability and are considered one possible cause of cancer progression. In prostate and breast cancer, centrosome over-duplication is correlated with lymph node and distant metastasis. In prostate cancers, centrosome amplification is a useful predictor of tumor recurrence, highlighting its potential role in promoting advanced disease. PAK6 was identified as an androgen receptor (AR) interacting protein. It has been determined that activated PAK6 plays an active role in promoting hormone-regulated prostate cancer metastatic phenotypes such as upregulating cell motility and invasiveness. Several lines of evidence indicate that PAK6 is overexpressed in advanced cancers including prostate, colon and liver cancers. The up-regulated expression of PAK6 in advanced diseases underscores its potential role in disease progression. Using a novel anti-PAK6 antibody readily recognizes the native PAK6 protein, we determined that PAK6 localizes at centrosome and in some cell types also at plasma membrane. This observation revised our previous view of PAK6 as a soluble cytoplasmic protein. Ectopically expression of PAK6 leads to hyperploidy in LNCap cells. We hypothesized that aberrant PAK6 expression plays a critical role in the development of hyperploidy. In current study, we characterized the role of PAK6 in the dysregulation of centrosome-triggered hyperploidy in cancer cells. PAK6 centrosome localization was determined by co-staining of PAK6 with centrosome marker γ-tubulin using immunofluorescence microscopy of a pair of mouse embryo fibroblasts (MEF) derived either from WT mice (MEF-WT) or from PAK6-null mice (MEF-KO). PAK6-centrosome association was also confirmed biochemically by co-fractionation of PAK6 with γ-tubulin in centrosomal subcellular-fractions using a sucrose gradient. Down-regulation of PAK6 expression by lentivirus mediated shRNA promotes centrosome amplification with increased centrosome size in LNCap and MCF7 cells. Accelerated microtubule aster regrowth following nocodazole-induced depolarization was observed in MEF KO cells in as compared to MEF WT cells. Reciprocally, the inhibitory effects of PAK6 overexpression on aster growth was demonstrated in tetracycline inducible U2OS cells. Our results indicate that PAK6 on one hand promotes aneuploidy, but on the other hand its expression suppresses centrosome amplification and microtubule dynamics. These apparent incompatible results suggest that PAK6 functions at multiple levels that in one hand it regulates centrosome amplification and microtubule dynamics, and on the other hand it may be involved in regulating the chromosome segregation and cytokinesis. The net effects of PAK6 expression may be context dependent. Citation Format: Houjun Jia, Danda Chapagai, John Mallow, Michael D. Perttunen, Vijaya Iragavarapu, Jianning Wei, Michael L. Lu. PAK6 localizes to centrosome and modulates centrosome homeostasis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 3453.
- Published
- 2018
14. Exogenous stem cell factor improves interstitial cells of Cajal restoration after blockade of c-kit signaling pathway
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Chunxue Li, Weidong Tong, Baohua Liu, Houjun Jia, Lin Zhang, and Timothy J. Ridolfi
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Mice, Inbred BALB C ,Stem Cell Factor ,Pathology ,medicine.medical_specialty ,Cellular differentiation ,Gastroenterology ,Cell Differentiation ,Stem cell factor ,Biology ,Interstitial Cells of Cajal ,Interstitial cell ,Interstitial cell of Cajal ,Cell biology ,Blockade ,Mice ,Proto-Oncogene Proteins c-kit ,symbols.namesake ,Kit signaling pathway ,symbols ,medicine ,Animals ,Immunohistochemistry ,Signal transduction ,Gastrointestinal Motility ,Signal Transduction - Abstract
Interstitial cells of Cajal (ICC) have been endowed with considerable intrinsic plasticity. Blockade of the c-kit signaling pathway results in the shift of ICC towards a smooth muscle-like phenotype. Little is known about stem cell factor (SCF), the ligand of c-kit, and the role it plays in the process of restoration. The aim of this study was to determine whether exogenous SCF can promote ICC replenishment following the blockade of c-kit signaling.Neutralizing anti-c-kit monoclonal antibody (ACK2) was administered to mice for 8 days after birth. Jejunal muscle strips were cultured up to 7 days. Electrical rhythmic changes were monitored and ICC were examined by immunohistochemistry. Expression of c-kit mRNA was detected by reverse transcriptase-polymerase chain reaction, and expression of Kit protein was detected by Western blot.When c-kit receptors were blocked, ICC nearly disappeared from the jejunum accompanied by the loss of electrical slow waves. By day 7, after in vitro culture with SCF (100 ng/ml), the amplitude of muscle strip slow waves was restored to 0.19 +/- 0.07 mV (p0.05), whereas the frequency recovered to 13.7 +/- 3.32/min (p0.01). Furthermore, labeling for c-kit(+) cells in the myenteric plexus increased and c-kit mRNA and protein expression were up-regulated compared to that of non-treatment with SCF.The c-kit signaling pathway, activated by SCF, is the critical pathway associated with the control of ICC survival and proliferation. The restoration of ICC number and jejunal electrical rhythm, resulting from blockade of the c-kit signaling pathway, could be facilitated by local SCF administration.
- Published
- 2010
15. Peritoneal catheter fixation to the abdominal wall in surgical catheter implantation to prevent malfunction
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Guohua Ding, Huiming Wang, Xifeng Lv, and Houjun Jia
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Catheter Obstruction ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Pain ,Peritoneal dialysis ,Catheterization ,Abdominal wall ,Fixation (surgical) ,Catheters, Indwelling ,Postoperative Complications ,Peritoneal Dialysis, Continuous Ambulatory ,Medicine ,Humans ,Renal Insufficiency, Chronic ,Peritoneal Cavity ,Retrospective Studies ,business.industry ,Abdominal Wall ,Hematology ,General Medicine ,Middle Aged ,Surgery ,Catheter ,medicine.anatomical_structure ,Nephrology ,Catheter-Related Infections ,Chronic renal failure ,Female ,business ,Peritoneal catheter - Abstract
Background: Catheter failure, especially catheter displacement and obstruction remain the major barriers for peritoneal dialysis. We have developed a new surgery technique of catheter fixation on the lower abdominal wall in the catheter planting to avert the catheter mechanical complications. Method: A retrospective study was performed on 93 patients; among them, 52 patients underwent the traditional method of surgery for catheter insertion and 41 patients received additional catheter fixation. Comparisons of complications including infection, leak, infusion pains, catheter displacement, and obstruction occurred during a follow-up period of 6 months, were made between the fixed and non-fixed groups. Results: Catheter fixation cost more time than the conventional operating procedure (94.2 w 14.6 min vs. 83 w 13.3 min, p = 0.043). Complications of infection, leak, and infusion pain that occurred in the fixed and unfixed groups are comparable. Catheter fixation reduced the complications of catheter displacement or obstruction to 0 episode in the fixed group, whereas those complications were encountered by 7 patients in the unfixed group (0/41 vs. 7/52, p = 0.022). All these 7 patients received re-exploration and catheter replacement with further catheter fixation. In the following time until now (ranging from 3 to 16 months), no catheter dysfunction was observed. Conclusions: These results suggest that catheter fixation is effective in preventing catheter displacement and obstruction in peritoneal dialysis. i 2014 S. Karger AG, Basel
- Published
- 2014
16. Geohazards Analysis of the Litang–Batang Section of Sichuan–Tibet Railway Using SAR Interferometry
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Xuguo Shi, Liming Jiang, Houjun Jiang, Xudong Wang, and Jinhu Xu
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Rainfall ,sand-sliding slopes ,seasonally frozen soil ,Sichuan–Tibet Railway (STR) ,the Luanshibao landslide ,Ocean engineering ,TC1501-1800 ,Geophysics. Cosmic physics ,QC801-809 - Abstract
The Sichuan–Tibet Railway (STR), as the second railway connecting the Tibet Autonomous Region with the rest of China, is recognized as a historical challenging project. There are widely distributed geohazards (e.g., landslides, sand-sliding slopes, collapses, ice avalanches, and debris flows) along the STR due to the significantly undulating terrains, unfavorable geological conditions, and intense tectonic movements. Therefore, it is necessary to identify the potential threats for the construction and regular operation of the STR. Interferometric synthetic aperture radar (InSAR) is a well-known tool for wide area geohazards detection with high precision. In this study, we employed small baseline subset analysis method to retrieve the ground displacement of the Litang–Batang section of the STR with C-band Sentinel-1 SAR images acquired from December 2015 to October 2019. The identified geohazards in this section are landslides, sand-sliding slopes, and seasonal frozen soils. High risk of geohazards is identified in the Maoyaba-Batang section through a hotspot and cluster analysis method. The head area of the ancient earthquake triggered Luanshibao landslide is identified active with displacement rate of −15 mm/yr, which might bring threat to the Sichuan–Tibet highway. Seasonal displacement due to freeze-thawing cycles of the seasonal frozen soils is detected in the alluvial area of the Litang River with maximum amplitude of 20 mm. Seasonal accelerations correlated with concentrated rainfall are identified on active slopes. Our study proved InSAR is an effective tool in detecting and monitoring mountainous unstable slopes.
- Published
- 2021
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17. A Parallel InSAR Phase Unwrapping Method Based on Separated Continuous Regions
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Jian Gao, Houjun Jiang, Zhongchang Sun, Ruisheng Wang, and Youmei Han
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phase unwrapping ,continuity distance ,separated continuous regions ,region residue pair ,heterogeneous residual diffusion ,parallel mode ,Science - Abstract
Phase unwrapping is an imperative step in interferometry processing that has a significant influence on the quality of subsequent products. Many existing phase unwrapping algorithms have been designed to solve for the unwrapped phase under the assumption that noisy areas with discontinuities are small or that reliable continuity can be recovered there. They attempt to restore the unwrapped phase by using continuity and data quality measures, such as residues. However, when the observing field is divided into separate zones of continuous phase due to a large range of noise, such as those caused by rivers or mountains, it is difficult to use traditional phase unwrapping techniques to recover global continuity in these noisy areas. To address this challenge, we present a two-dimensional parallel phase unwrapping method that is designed to handle cases where the continuity of the phase is separated by closed noisy loops. Based on continuity distances, this method aims to identify continuous regions that are free of hidden phase discontinuities and restore phase continuity between the separated regions. A heterogeneous residual diffusion scheme is used to restore the unwrapped phase outside continuous regions. The parallel algorithm for extracting continuous regions, restoring continuity between the regions, and diffusing residuals was implemented on a GPU device to increase the processing efficiency. We applied our method to typical TanDEM-X data covering rivers, islands, and mountains and demonstrated that it is a promising solution for large-scale, heavily noisy phase unwrapping problems.
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- 2023
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18. Effects of LPS, PLA(2) and OFR on proton translocation across inner mitochondrial membrane and H(+)-ATPase in the liver
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Songmin, Lu, Heming, Yang, Shuangming, Song, Jiancang, Liu, Ping, Li, Zhihong, Wan, and Houjun, Jia
- Abstract
OBJECTIVE: To elucidate the effects of lipopolysaccharide (LPS), phospholipase A(2) (PLA(2)) and oxygen free radical (OFR) on proton transmembrane translocation and H(+)-ATPase. METHODS: The normal rats were sacrificed for preparetion liver mitochondria and submitochondrial particles for experiments in vitro. Submitochondrial particles were incubated with LPS (100mgr;g/mL), PLA(2) (10 u/mL) and FeSO(4)/Vit C (30/90mgr;mol/L) at 30 degrees C for 30 min. The proton translocation of submitochondrial particles (SMPs) were assayed with the fluorescent probe ACMA (9-amino-6-chloro-2 methoxya cridine). The mitochondria were incubated with different concentration of LPS, PLA(2) and FeSO(4)/Vit C. The H(+)-ATPase, PLA(2) and malondialdehyde (MDA) were assayed. RESULTS: The fluorescent quenching of ACMA and H(+)-ATPase activity in high dose was significantly decreased after treatment with LPS, PLA(2), FeSO(4)/Vit C (P0.05). The mitochondrial PLA(2) activity and MDA content were significantly increased after treatment with LPS (P0.01). CONCLUSIONS: FeSO(4)/Vit C in low dose causes increases H(+)-ATPase activity. LPS, PLA(2), FeSO(4)/Vit C might be the important factors changing H(+)-ATPase and proton translocation across the membrane.
- Published
- 2002
19. An Efficient Maximum Likelihood Estimation Approach of Multi-Baseline SAR Interferometry for Refined Topographic Mapping in Mountainous Areas
- Author
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Yuting Dong, Houjun Jiang, Lu Zhang, and Mingsheng Liao
- Subjects
multi-baseline InSAR ,maximum likelihood (ML) ,DEM ,L-band ,ALOS/PALSAR ,Science - Abstract
For InSAR topographic mapping, multi-baseline InSAR height estimation is known to be an effective way to facilitate phase unwrapping by significantly increasing the ambiguity intervals and maintaining good height measurement sensitivity, especially in mountainous areas. In this paper, an efficient multi-baseline SAR interferometry approach based on maximum likelihood estimation is developed for refined topographic mapping in mountainous areas. In the algorithm, maximum likelihood (ML) height estimation is used to measure the topographic details and avoid the complicated phase unwrapping process. In order to be well-adapted to the mountainous terrain conditions, the prior height probability is re-defined to take the local terrain conditions and neighboring height constraint into consideration in the algorithm. In addition, three strategies are used to optimize the maximum likelihood height estimation process to obtain higher computational efficiency, so that this method is more suitable for spaceborne InSAR data. The strategies include substituting a rational function model into the complicated conversion process from candidate height to interferometric phase, discretizing the continuous height likelihood probability, and searching for the maximum likelihood height with a flexible step length. The experiment with simulated data is designed to verify the improvement of the ML height estimation accuracy with the re-defined prior height distribution. Then the optimized processing procedure is tested with the multi-baseline L-band ALOS/PALSAR data covering the Mount Tai area in China. The height accuracy of the generated multi-baseline InSAR DEM can meet both standards of American DTED-2 and Chinese national 1:50,000 DEM (mountain) Level 2.
- Published
- 2018
- Full Text
- View/download PDF
20. Landslide Displacement Monitoring with Split-Bandwidth Interferometry: A Case Study of the Shuping Landslide in the Three Gorges Area
- Author
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Xuguo Shi, Houjun Jiang, Lu Zhang, and Mingsheng Liao
- Subjects
landslide ,the Three Gorges ,corner reflector ,split-bandwidth interferometry ,Science - Abstract
Landslides constitute a major threat to people’s lives and property in mountainous regions such, as in the Three Gorges area in China. Synthetic Aperture Radar Interferometry (InSAR) with its wide coverage and unprecedented displacement measuring capabilities has been widely used in landslide monitoring. However, it is difficult to apply traditional InSAR techniques to investigate landslides having large deformation gradients or moving primarily in north-south direction. In this study, we propose a time series split-bandwidth interferometry (SBI) procedure to measure two dimensional (azimuth and range) displacements of the Shuping landslide in the Three Gorges area with 36 TerraSAR-X high resolution spotlight (HS) images acquired from February 2009 to April 2010. Since the phase based SBI procedure is sensitive to noise, we focused on extracting displacements of corner reflectors (CRs) installed on or surrounding the Shuping landslide. Our results agreed well with measurements obtained by the point-like targets offset tracking (PTOT) technique and in-situ GPS stations. Centimeter level accuracy could be achieved with SBI on CRs which shows great potential in futures studies on fast moving geohazards.
- Published
- 2017
- Full Text
- View/download PDF
21. Using Open-Source Components to Process Interferometric TerraSAR-X Spotlight Data
- Author
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Michael Jendryke, Timo Balz, Houjun Jiang, Mingsheng Liao, and Uwe Stilla
- Subjects
Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Cellular telephone services industry. Wireless telephone industry ,HE9713-9715 - Abstract
We address the processing of interferometric TerraSAR-X and TanDEM-X spotlight data. Processing steps necessary to derive interferograms at high spatial resolution from bi- and monostatic satellite images will be explained. The spotlight image mode is a beam steering technique focusing the antenna on a specific ground area. This results in a linear Doppler shift frequency in azimuth direction, which has to be matched to the master image. While shifting the interpolation kernel in azimuth during resampling, the frequency spectrum of the slave image is aligned to the master image. We show how to process bistatic TanDEM-X images and propose an integrated processing option for monostatic TerraSAR-X data in the Delft Object-oriented Radar Interferometric Software (DORIS). The paper focuses on the implementation of this algorithm for high-resolution spotlight InSAR in a public domain tool; hence, it becomes available to a larger research community. The results are presented for three test areas: Uluru in Australia, Las Vegas in the USA, and Lüneburg in Germany.
- Published
- 2013
- Full Text
- View/download PDF
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